豚草条纹叶(虫甲)(鞘翅目:叶(虫甲)科)的寄主专一性测定     

万方浩  王韧  邱式邦 《中国生物防治》1989,(1)

1987年从苏联、加拿大引进原产北美的豚草天敌—豚草条纹叶(虫甲),在室内用具有代表性的23科72种主要经济作物、粮食作物、观赏植物及与豚草近缘或形态上相似的植物,进行了食性测定。结果表明,豚草条纹叶(虫甲)食性专一,仅取食豚草,不取食其它任何供试植物,在我国可以安全利用。在饥饿条件下,一龄幼虫的耐饥能力为3.1天,雌、雄成虫的耐饥能力分别为14和10.2天。  相似文献   

豚草条纹叶(虫甲)的生物学特性     

万方浩  王韧 《中国生物防治》1989,(2)

豚草条纹叶(虫甲)在北京地区年发生3代,以成虫入土越冬。越冬成虫于4月底至5月上出土活动。1、2、3代成虫分别于6月中下、7月下至8月初和9上中旬羽化出土。在26±1℃恒温条件下,雌雄成虫的寿命为82.5和67.8天。产卵前期16.5天,产卵期49.1天,一生产卵394.5粒。成虫一般仅在产卵后第30天前后出现一次明显的产卵高峰。卵、幼虫、蛹的发育历期分别为5.0、13.9和12.9天,在保温条件下,卵的孵化率最高,达92％。幼虫和蛹的存活率在16±1℃、26±1℃恒温及20～27℃室温条件下,差异不明显,最高78％,最低66.7％。  相似文献   

Growth of the mycoparasitic fungus Verticillium biguttatum from different geographical origins at near-minimum temperatures     

P. H. J. F. van den Boogert  T. A. W. M. Saat 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(2):115-124

Sclerotia ofRhizoctonia solani collected from potato tubers from different countries were assayed for the presence of mycoparasites. Among the mycoparasites observedVerticillium biguttatum predominated. Its geographical distribution was not restricted to certain latitudes or soil types;V. biguttatum occurred worldwide in potato fields.The minimum growth temperature of 57V. biguttatum isolates was found to be in the narrow range from 10 to 13°C, irrespective of their geographical origin. A non-linear logistic growth model was used to describe the radial growth onRhizoctonia mycelium and nutrient agar plates. At near-minimum temperature the maximum colony radii varied considerably; they were up to 3.8 times that of the reference isolate M73. Based on parameter values for logistic growth, fast-and slow-growing isolates could be distinguished. Although the growth properties ofV. biguttatum isolates from different locations varied, the presence of fast- and slow-growing isolates was not restricted to particular areas and both types could be found in the same field. However, bioassays with selected fast- and slow-growing isolates do not support the assumption that growth at near-minimum temperatures is a relevant criterion for screening isolates ofV. biguttatum in terms of effectiveness for biological control ofR. solani.  相似文献   

柑桔“以螨治螨”生物防治技术研究   总被引：2，自引：0，他引：2  

李惠陵李国强  李华梁铭生  李子元李艳红 《昆虫天敌》2005,27(3):134-139

柑桔病虫害发生种类多，为害重，生产上喷药次数多，果品存在农药残留高，为了使我市柑桔达到无公害生产水平，减少化学农药使用量，提高柑桔品质，笔者通过释放捕食螨Amblyseius spp．建立捕食螨种群，同时结合使用生物农药和电子灭虫灯，以及白花草Ageratum conyzoides L．等栽培措施，达到了长期持续控制柑桔病虫发生的目的，而且减少我县柑桔生产的农药残留问题，降低生产成本，减少环境污染。  相似文献   

Random amplified polymorphic DNA markers reveal low genetic variation and a single dominant genotype in Eichhornia crassipes populations throughout China   总被引：8，自引：0，他引：8  

M-X REN  Q-G ZHANG  & D-Y ZHANG 《Weed Research》2005,45(3):236-244

Genetic variation within and between 34 populations of Eichhornia crassipes (water hyacinth) in China was surveyed using random amplified polymorphic DNA (RAPD) markers. A total of 1009 individuals were analysed, for which 12 RAPD primers amplified 69 reproducible bands, with 22 (32%) being polymorphic. The percentage of polymorphic loci (p) within a population ranged from 4.4% to 17.4%, and the mean Nei's gene diversity (H_e) was 0.046 ± 0.0145, indicating a low genetic diversity of E. crassipes in China. Each population contained at least four RAPD phenotypes (genotypes), and the same particular genotype was invariably dominant in all the populations sampled. The mean proportion of distinguishable genotypes was 0.29. Analysis of molecular variance revealed a large proportion of genetic variation (83.9%) residing within populations and a slightly larger proportion (88.1%) within localities, indicating a low genetic differentiation of E. crassipes populations, both locally and regionally. Human-mediated dispersal, vigorous clonal growth, and a generally low level of sexual reproduction were thought to be responsible for such a pattern of genetic structure.  相似文献   

Effectiveness of Coniothyrium minitans and Trichoderma atroviride in suppression of sclerotinia blossom blight of alfalfa   总被引：3，自引：0，他引：3  

G. Q. Li  H. C. Huang †  S. N. Acharya  R. S. Erickson 《Plant pathology》2005,54(2):204-211

The effects of the mycoparasites Coniothyrium minitans and Trichoderma atroviride on the suppression of alfalfa blossom blight caused by Sclerotinia sclerotiorum were evaluated under indoor and field conditions. When T. atroviride (9·0 × 10⁴ conidia/floret) + S. sclerotiorum (6·0 × 10³ ascospores/floret) or C. minitans (9·0 × 10⁴ conidia/floret) + S. sclerotiorum (6·0 × 10³ ascospores/floret) were applied to detached young alfalfa florets, T. atroviride effectively inhibited saprophytic growth of S. sclerotiorum, whereas C. minitans showed no inhibition under the same conditions. When T. atroviride (6·9 × 10⁴ conidia/floret) + S. sclerotiorum (6·0 × 10³ ascospores/floret) or C. minitans (6·9 × 10⁴ conidia/floret) + S. sclerotiorum (6·0 × 10³ ascospores/floret) was applied to young alfalfa petals in vivo just after pollination, the percentage of pod formation was higher for T. atroviride+S. sclerotiorum than that for C. minitans+S. sclerotiorum, and the percentage of pod rot was lower for T. atroviride+S. sclerotiorum than that for C. minitans+S. sclerotiorum. However, when they were applied to senescent petals attached to developing pods of alfalfa at 9·2 × 10⁴ conidia/floret together with S. sclerotiorum at 4·5 × 10³ ascospores/floret at 14 days after pollination, C. minitans was more effective than T. atroviride in suppressing sclerotinia pod rot and seed rot of alfalfa. Field experiments showed that three applications of C. minitans (5·4 × 10⁶ conidia mL⁻¹) or T. atroviride (5·4 × 10⁶ conidia mL⁻¹) at a 7-day interval to blossoms of alfalfa effectively suppressed sclerotinia pod rot in two out of three annual trials. Coniothyrium minitans effectively suppressed sclerotinia seed rot in all three years, whereas T. atroviride was not effective against seed rot in any of the trial years. The efficacy of C. minitans was not significantly different (P > 0·05) from benomyl (250 µg ai mL⁻¹). This study suggests that C. minitans has potential as a biocontrol agent to control blossom blight of alfalfa caused by S. sclerotiorum.  相似文献   

Control of Phytophthora cryptogea in the hydroponic forcing of witloof chicory with the rhamnolipid-based biosurfactant formulation PRO1     

K. De Jonghe †  I. De Dobbelaere  R. Sarrazyn  M. Höfte 《Plant pathology》2005,54(2):219-226

Rhamnolipids, extracellular metabolites of Pseudomonas aeruginosa with surfactant properties, proved to be very effective in controlling the spread of brown root rot disease caused by Phytophthora cryptogea in the hydroponic forcing system of witloof chicory ( Cichorium intybus var. foliosum ). The biosurfactant was applied as the product PRO1, a formulation of 25% rhamnolipids in oil. Both an in vitro screening and in vivo experiments in a mini-hydroponic system demonstrated the ability of PRO1 to control brown root rot. A 25  µ g mL⁻¹ rhamnolipids nutrient solution was enough to obtain good control of an artificial infection with a zoospore suspension of P. cryptogea . The biosurfactant PRO1 performed well in a semicommercial system under growers' conditions. A treatment of 25  µ g mL⁻¹ rhamnolipids (100  µ g mL⁻¹ PRO1) reduced the disease incidence significantly in two independent experiments. However, PRO1 was not effective when a mycelial suspension was used as inoculum. Rhamnolipids have good potential to limit the spread of P. cryptogea in the hydroponic forcing system of witloof chicory, and can be used as a preventive measure against brown root rot.  相似文献   

N-｛5-[1-(2,4-二氯苯氧)乙基]-1,3,4-噻二唑-2-基｝ -N''-芳酰基硫脲的合成及其生物活性          下载免费PDF全文

龚银香  王子云  王胜  汪焱钢 《农药学学报》2005,7(4):361-363

通过2-氨基-5- -1,3,4-噻二唑与芳酰基异硫氰酸酯反应,合成了12 个新的芳酰基硫脲,采用核磁共振氢谱、红外光谱和元素分析确证了它们的结构。初步的生物活性测定试验表明,部分化合物具有良好的植物生长调节活性,其中化合物 2c、2j 具有较好的生长素活性,其促进率分别为26.6%与26.9%,但不及对照β-吲哚乙酸。  相似文献   

柽柳有效天敌昆虫筛选标准的建立   总被引：1，自引：1，他引：1  

孟玲  李保平 《中国生物防治》2005,21(3):192-195

参照前人对杂草天敌筛选标准、植物与昆虫互作理论和近年来对柽柳原产地主要天敌生物学特性的研究结果，制定出柽柳天敌遴选量化标准，共计10项特征，满分为47分。并以此对取食柽柳的12种天敌进行了筛选。柽柳条叶甲得分最高（43分），其次是斯氏伞锥象（41分），推荐这2种天敌昆虫作为首选天敌引入美国做进一步评价。  相似文献   

球孢白僵菌对马铃薯块茎蛾的毒力及其与常用农药的生物相容性测定   总被引：2，自引：1，他引：2       下载免费PDF全文

李正跃  ;张青文 《植物保护》2005,31(3):57-61

室内测定了从云南分离的7株球孢白僵菌对马铃薯块茎蛾幼虫的毒力,结果表明,在接菌孢子浓度为10⁵个/mL和10⁷个/mL下,接种后7d马铃薯块茎蛾幼虫累积死亡率分别为23.3%～70.0%和33.3%～86.7%,添加吐温80的清水对照死亡率仅为3.3%。筛选出毒力较高的Bb7001、Bb7004、Bb8001 3个菌株,其中菌株Bb8001对马铃薯块茎蛾幼虫的毒力最高,在孢子浓度1×10⁵、1×10⁷个/mL下,第7天的累计死亡率分别为70.0%和86.7%,其毒力回归方程Y=0.366X+3.409(R=0.956),剂量效应LC₅₀=2.24×10⁴个/mL,孢子浓度10⁷个/mL下的LT₅₀为4.3d。测定了毒力较高的菌株与12种农药的生物相容性,结果表明在供试农药推荐用量及其101、00倍稀释液下,球孢白僵菌Bb7001、Bb7004、Bb8001的3个菌株孢子在含所选农药的营养液中生物相容性良好的农药有:苏云金芽孢杆菌可湿性粉剂(Bt)、10%吡虫啉可湿性粉剂、2.5%菜喜悬浮剂及松毛虫病毒可湿粉剂,可配伍使用;而阿维菌素、农安、卡死克、抑太保对球孢白僵菌有很强的抑制作用,应避免配伍使用。  相似文献