不同木薯品种块根成分的累积变化规律     

张树河  李海明  李瑞美  李和平  潘世明 《福建省农科院学报》2014,(2):139-142

以F15、F901、G15、瑞士N4和GR911等木薯品种为材料,在块根膨大期对其干物质、蛋白质、淀粉、可溶性总糖含量以及收获期对产量进行测定。结果表明：木薯块根干物质和淀粉的积累与生长期呈一定的正相关,随着时间的延长而升高;蛋白质含量随着时间的延长均呈先升后降的趋势,在11月份达到最高值;依据淀粉含量变化和块根产量表现, F15和F901淀粉含量连续保持上升趋势,建议采收期在1月份, G15和瑞士N4淀粉含量在12月份增长缓慢或不增长,建议采收期在12月份。  相似文献   

木薯叶多酚氧化酶的提取及其酶学性质研究     

侯杰严东  马万良杨洋 《广东农业科学》2014,41(2):111-115

以木薯叶为原料,研究木薯叶多酚氧化酶PPO的最佳提取条件与酶学性质。结果表明,木薯叶PPO 的 最佳提取条件为磷酸缓冲液PBS的pH 值6.8料液比4 mL/g提取时间6 h;木薯叶PPO 酶促反应产物在399 nm 处有最大吸收峰,以邻苯二酚为底物的酶促反应的米氏常数Km=0.8 mol/L动力学方程为V=2000[S]/0.8+[S]该酶最 适温度为37益,最适pH 值为7.0。  相似文献   

蚯蚓处理热带农业固体废弃物 木薯渣的适宜条件初探     

陈键  陈淼  刁晓平  俞花美  葛成军 《广东农业科学》2014,41(12):183-188

采用室内模拟培养的方法,利用蚯蚓处理热带农业固体废弃物木薯渣,研究处理过程中蚯蚓生长繁殖 状况及处理前后混合废弃物的理化性质,探讨蚯蚓处理热带农业废弃物的适宜条件。结果表明,热带农业废弃物木 薯渣和砖红壤的不同配比基质中赤子爱胜蚓的生长繁殖良好,各试验组蚯蚓的生长繁殖与基质性质及环境条件之 间存在明显关系,其在30%砖红壤+70%木薯渣的处理中繁殖情况最好,产茧数量最多。蚯蚓在温度25益、含水率 70%、pH 7.9、接种密度10 条/60g 干重基质时具有最好的生长繁殖效率,同时赤子爱胜蚓对此类热带农业废弃物也 具有较好的处理效果,不同配比基质经蚯蚓处理后速效氮和速效磷含量明显增加。  相似文献   

广西食用木薯淀粉卫生质量检测结果分析     

李健  ;古碧  ;龙罡  ;黄一帆 《广西热带农业》2014,(3):41-46

目的：检测分析广西武鸣、隆安、北海、崇左、桂林和梧州2011～2012、2012～2013年度食用木薯淀粉卫生质量.方法：根据GB/T 5009测定食用木薯淀粉中砷、铅、铬、氢氰酸和黄曲霉素B1含量,根据GB/T 22427.13测定二氧化硫残留量,根据GB 4789测定食用木薯淀粉中菌落总数、霉菌数、致病菌.结果：60份食用木薯淀粉样品中,铅含量达标样品合格率为93.3％,二氧化硫残留量合格率为56.7％,砷、铬、氢氰酸和黄曲霉素B1合格率为100％,微生物指标合格率为100％.结论：淀粉企业需继续加强食品安全生产知识宣传,改善工艺,以求生产符合国家标准的食用木薯淀粉.  相似文献   

木薯MePP2CAa基因克隆、表达及蛋白互作分析          下载免费PDF全文

曾坚  李丽珍  沈梓欣  林墁  刘博婷  吴春来  李冰  胡伟  曾力旺 《华中农业大学学报》2024,43(1):141-148

为探究2C型蛋白磷酸酶（protein phosphatase 2C， PP2C）在木薯响应非生物胁迫过程中的作用，利用木薯Arg7叶片cDNA扩增MePP2CAa基因，分析该基因序列、启动子活性、不同逆境和激素处理下的表达模式以及与ABA受体PYLs之间的互作关系。序列分析结果显示，MePP2CAa基因全长1 311 bp，编码436个氨基酸，具有PP2C家族的结构域特征，与橡胶树和麻风树的PP2C序列同源性最高，分别为78.95%和74.09%，在C端保守；qRT-PCR分析结果显示，MePP2CAa基因在木薯储藏根中的表达显著高于茎、叶中的表达量；不同逆境和激素处理结果显示，甘露醇、NaCl、ABA、MeJA、低温和SA处理可以显著诱导MePP2CAa基因的表达；MePP2CAa基因启动子序列分析显示，启动子包含ABA应答元件（abscisic acid responsive element，ABRE）、MeJA响应元件、干旱诱导元件等；酵母双杂交结果显示MePP2CAa能够与MePYL1互作。以上结果表明，MePP2CAa基因可能响应木薯的非生物胁迫。  相似文献   

甘薯渣蕈菌发酵饲料的研究     

赵启美  何佳 《河南农业科学》2002,(5):28-30

采用固液结合，利用鸡腿菇（瑞10）对甘薯渣进行发酵。发酵10天，产物粗蛋白含量可达14.1%，比原料本身的粗蛋白含量提高了246.35，比所用培养基的粗蛋白含量提高58.4%。发酵产物的粗纤维含量由原料中的25.03%下降至14.2%，降解率为43.1%。且具有鸡腿菇特有的清香气味。  相似文献   

Nuclear DNA content and in vitro induced somatic polyploidization cassava (Manihot esculenta Crantz) breeding     

F. Awoleye  M. van Duren  J. Dolezel  F. J. Novak 《Euphytica》1994,76(3):195-202

Summary The diploid (2C) amount of DNA in cassava (Manihot esculenta Crantz) is 1.67 picograms (pg) per cell nucleus. This value corresponds to 772 mega-base pairs in the haploid genome. The size of the nuclear genome in cassava is very small in comparison with other Angiosperms. Flow cytometry techniques were used to screen ploidy levels in a large population of in vitro plantlets treated with colchicine and oryzalin (3,5-dinitro-N⁴,N-dipropylsulphate). Culture of axillary node cuttings for 48 hours in liquid medium supplemented with 2.5 to 5.0 mM colchicine in combination with 2% dimethyl sulfoxide (DMSO) resulted in a high frequency (23 to 42%) of non-chimeric tetraploids in the V₃ generation. Although mixoploidy may persist in as many as four cycles of vegetative propagation of node cuttings, solid (non-chimeric) tetraploids can be identified by flow cytometry among in vitro plantlets and then rapidly propagated for field testing. A somatic polyploidization system is proposed for implementation in cassava breeding programmes.Dedicated to the memory of the late Dr. Novak. Correspondence to M. van Duren  相似文献   

Isozyme analysis of an active cassava germplasm bank collection     

Angélica V. V. Montarroyos  Marco A. G. de Lima  Elton O. dos Santos  José G. E. de França 《Euphytica》2003,130(1):101-106

  相似文献   

Somatic embryogenesis from floral tissue of cassava (Manihot esculenta Crantz)   总被引：4，自引：0，他引：4  

Barbara Woodward  Johanna Puonti-Kaerlas 《Euphytica》2001,120(1):1-6

The aim of this study was to examine the embryogenic potential of floral material of the cassava cultivar MCOL 1505. Macerated immature inflorescences were found to be highly embryogenic, with almost 78% of the explants producing somatic embryos. Somatic embryos were also produced from whole male florets and half florets although at much lower rates. No regeneration was obtained from anther, microspore or floret wall tissue. Somatic embryos derived from immature inflorescences were regenerated via organogenesis and the plants derived from this process were assessed in terms of phenotype and ploidy level. If haploid plants could be produced by this method, this would have significant implications in assisting traditional cassava breeding, as this would allow homozygosity to be reached more rapidly. In a crop such as cassava, which is highly heterozygous in nature, the use of haploids in a breeding programme could considerably shorten the time taken to produce new desirable cultivars. This is the first report on plant regeneration through somatic embryogenesis from floral tissue of cassava. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Genomic and biological diversity of the African cassava geminiviruses     

J.S. Pita  V.N. Fondong  A. Sangaré  R.N.N. Kokora  C.M. Fauquet 《Euphytica》2001,120(1):115-125

The virological situation of cassava in Africa is increasing in complexity due to the number and types of viruses isolated from different locations within the continent. Here, we report the complete nucleotide sequences of both A and B components of two geminivirus species infecting cassava in the Ivory Coast and review the current knowledge of the molecular and biological diversity of the African cassava geminiviruses. As a whole, newly obtained sequences are compared with those of the African cassava mosaic geminiviruses identified to date. Results indicate that all isolates of African cassava mosaic virus (ACMV), irrespective of their geographical origin are clustered together with little or no variation in their genomic sequence. On the contrary, the genomes of the East African cassava mosaic virus (EACMV) are more genetically diverse due to the frequent occurrence of recombinations within their two components. Indeed, the EACMV-like viruses vary so much that their classification is becoming problematic. In addition, there is also a large range of phenotypic symptom variation for each of these virus species, irrespective of the location of isolation. Furthermore, it has been shown that ACMV and EACMV can be synergistic in cassava, resulting in a greater DNA accumulation and consequently inducing severe symptoms. For all these reasons, this paper initiates a discussion concerning the species demarcation for cassava geminivirus. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献