乌龙茶水提取物对人大肠癌LOVO细胞增殖及凋亡的影响     

王丽丽  蔡淑娴  薛志慧  刘仲华 《福建农林大学学报(自然科学版)》2016,(6):638-643

以赛珍珠5800铁观音为材料,测定乌龙茶中水浸出物、茶多酚、游离氨基酸、可溶性糖、黄酮类物质、没食子酸、8种儿茶素和3种生物碱的含量;并将0、0.2、0.4、0.6、0.8和1.0 mg·m L~(-1)乌龙茶水提取物作用于体外培养的人大肠癌LOVO细胞中,分别处理24和48 h后,采用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率,并在倒置显微镜下观察细胞形态的变化;以0.6和0.8 mg·m L~(-1)乌龙茶水提取物处理LOVO细胞48 h,采用流式细胞仪检测细胞凋亡率,扫描电镜下观察细胞凋亡情况.结果表明,乌龙茶中各化学成分的含量均较高,水浸出物含量达40.8%以上,茶多酚含量14.2%,游离氨基酸含量1.8%,可溶性糖含量6.9%,黄酮类物质含量0.7%,儿茶素总量12.2%,咖啡碱含量1.7%,这些成分为其品质保证和功效发挥奠定了一定的物质基础.MTT法试验结果表明:0.2 mg·m L~(-1)乌龙茶水提取物对LOVO细胞生长无抑制作用;处理24和48 h后,0.4~1.0 mg·m L~(-1)乌龙茶水提取物对LOVO细胞生长的抑制率分别达13.9%~79.6%和16.4%~89.2%.0.6和0.8mg·m L~(-1)乌龙茶水提取物处理下LOVO细胞凋亡率分别为60.4%和62.3%,且低含量下细胞凋亡主要呈早期凋亡形式.扫描电镜观察显示,0.6和0.8 mg·m L~(-1)乌龙茶水提取物处理48 h后LOVO细胞出现明显的凋亡现象,细胞体积缩小,细胞间隙增大,表面微绒毛断裂、减少甚至消失,细胞膜表面出现塌陷,产生类似空洞的结构等.因此,乌龙茶水提取物能有效抑制人大肠癌LOVO细胞增殖,并可诱导其凋亡的发生.  相似文献   

3种血清肿瘤标志物在诊断大肠癌中的价值     

陈恺杰 《湛江医学院学报》2005,23(4):384-385

目的：探讨癌胚抗原（CEA）、糖链抗原（CA199．casbohydrate antigen19-9）和CA242（casbohydrate antigen 242）在大肠癌的临床价值。方法：采用微粒酶免疫法（MEIA）检测30例大肠良性疾病及45倒大肠癌患者血清CEA,CA199和CA242的水平。结果：大肠癌患者与大肠良性疾病患者的CEA,CA199和CA242水平比较差异有显著性（P〈0．01～0．05）。血清CEA,CA199和CA242的水平随大肠癌病程的进展而逐渐升高;3种肿瘤标志物中以CA242敏感性和特异性最高．分别为60．3％和73．3％．3种肿瘤标志物对诊断的敏感性与大肠癌的分期有关;联合检测可使诊断的敏感性提高至83．6％。结论：血清CEA,CA199和CA242水平测定可应用于大肠癌的诊断和疗效的观察,并可用于监测肿瘤的复发和转移。肿瘤标志物的联合检测有助于大肠癌的早期诊断,并增加诊断的敏感性和特异性。  相似文献   

The Early Effects of Clostridium perfringens Type D Epsilon Toxin in Ligated Intestinal Loops of Goats and Sheep   总被引：2，自引：0，他引：2  

Fernandez Miyakawa ME  Uzal FA 《Veterinary research communications》2003,27(3):231-241

Clostridium perfringens type D produces enterotoxaemia in goats, sheep and other animals. The disease is caused by C. perfringens epsilon toxin and, while enterotoxaemia in goats is usually characterized by enterocolitis, the disease in sheep is characterized by systemic lesions (such as lung and brain oedema) with minor and inconsistent changes observed in the intestine. A possible explanation for these differences is that epsilon toxin is more promptly absorbed by the ovine than by the caprine intestine. In an attempt to clarify this, we examined the early effects of epsilon toxin on caprine and ovine intestine. Intestinal loop assays were performed to analyse the physiological and morphological changes induced by epsilon toxin in the intestine of these species. Fluid accumulation was observed in caprine and ovine ileum and colon treated with epsilon toxin. Ileal loops from goats treated with epsilon toxin retained sodium and water earlier than ovine ileal loops treated with the same toxin. Histological analysis showed morphological alterations in the colon of both species as early as 2 h after the commencement of epsilon toxin treatment; these changes were more marked in goats than in sheep. No morphological changes were observed in the ileum of either species after 4 h incubation with epsilon toxin. These results suggest that epsilon toxin modifies ion and water transport in the small and the large intestine of goats and sheep through different mechanisms.  相似文献   

Operative factors associated with short‐term outcome in horses with large colon volvulus: 47 cases from 2006 to 2013          下载免费PDF全文

L. M. Gonzalez  C. A. Fogle  W. T. Baker  F. E. Hughes  J. M. Law  A. A. Motsinger‐Reif  A. T. Blikslager 《Equine veterinary journal》2015,47(3):279-284

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PREVALENCE AND SIGNIFICANCE OF AN ULTRASONOGRAPHIC COLONIC MUSCULARIS HYPERECHOIC BAND PARALLELING THE SEROSAL LAYER IN DOGS          下载免费PDF全文

Hock Gan Heng  Chee Kin Lim  Margaret A. Miller  Meaghan M. Broman 《Veterinary radiology & ultrasound》2015,56(6):666-669

The muscularis layer of the canine colon has been reported to appear homogeneously hypoechoic on ultrasonography. A hyperechoic band in the muscularis layer paralleling the serosal surface has been observed by authors in routine canine abdominal ultrasound examinations. The purpose of this prospective and retrospective cross‐sectional study was to determine the prevalence of this lesion, characterize its ultrasonographic and postmortem histologic features, and correlate its presence with clinical signs of gastrointestinal disease. In the prospective study, all dogs that underwent routine abdominal ultrasonography by one of two observers during a 4‐week period were included without any exclusion criteria. One observer reviewed ultrasound images and recorded the presence or absence of this lesion and its distribution, e.g. focal (< 2 cm long) or diffuse (> 2 cm long). In the retrospective study, all dogs that had both abdominal ultrasonography and necropsy from January 2011 to December 2013 were included without any exclusion criteria. Histologic examinations were performed by two observers and Masson's trichrome stain was used to identify fibrous collagen. Prevalence for the hyperechoic band was 32% in the prospective and 4.8% in the retrospective sample populations, respectively. The hyperechoic band appeared as diffuse, focal, or a combination of both. Histologic sections were available for six dogs. In a few cases, the lesion corresponded to the presence of fibrous tissue in the myenteric plexus or in the tunica muscularis. None of the dogs had a history of diarrhea. Findings supported the hypothesis that a colonic muscularis hyperechoic band paralleling the serosal layer in dogs could be a normal variant rather than a marker of disease.  相似文献   

Rupture of the mesocolon as a cause of recurrent colic in a showjumper mare     

F. Torre  J. Gasparin  M. B. Andreasi 《Equine Veterinary Education》2010,22(8):403-407

An 11‐year‐old Italian Saddlebred showjumper mare was referred for investigation of recurrent colic. The mare had undergone surgery for left dorsal displacement of the ascending colon 5 years previously and had subsequently experienced several episodes of colic that had responded to medical treatment. Due to deterioration of the mare's clinical condition in the last episode, characterised by unrelenting pain and worsening of the cardiovascular parameters, the mare underwent repeat surgery for suspected colonic displacement. Exploratory laparotomy revealed a complete rupture of the mesocolon of the ascending colon. The mesocolon was repaired and the mare recovered uneventfully. She returned to training and competition and only a single episode of mild transient colic was recorded in the follow‐up.  相似文献   

Volvulus of the colon in a Cat     

Kenneth J. Drobatz DVM  Dez Hughes BVSc  Chris Hill VMD  Lynn Walker VMD 《Journal of Veterinary Emergency and Critical Care》1996,6(2):99-102

We report the first case of colonic volvulus in a cat. A 12-year old spayed, female domestic shorthair cat with chronic bowel disease presentated with one-day history of lethargy and anorexia. Physical examination abnormalities included cachexia, poor perfusion, and poor skin turgor. A large, non painful, gas-filled, tubular structure, occupying the majority of the abdominal cavity was noted by palpation. Abdominal radiographs revealed a gas-filled displaced colon. Exploratory laparotomy confirmed a volvulus at the root of the colonic mysetery, along with a partial torsion of the distal colon. The majority of the colon was determined to be necrotic and was surgically resected. Due to persistent hypotension and perceived poor prognosis, the cat was euthanized.  相似文献   

Surgical Removal of a Descending (Small) Colon Foreign Body through a Secondary Paramedian Approach     

Antonio Cezar de Oliveira Dearo  Roberta Garbelini GomesRafael Goulart Araújo  DVM  Peter ReichmannMariana Cosenza  DVM  Mariana Marcantonio Coneglian  DVM 《Journal of Equine Veterinary Science》2009

Intestinal obstruction due to foreign body ingestion is one cause of colic in horses. Although it occurs more frequently in juveniles, adult horses also may be affected. The small colon is a common location in which foreign bodies can be found. Depending on the location of the obstruction in the small colon, different techniques to dislodge the foreign body toward areas where it can be safely exteriorized and removed can be attempted. Alternatively, different approaches to the abdomen can be performed to facilitate exteriorization of the obstructed segment and removal of the foreign body. This paper describes a case of large and small colon obstruction in an adult horse caused by two distinct foreign bodies. The large colon obstruction was removed through a pelvic flexure enterotomy using the ventral midline approach, and the small colon obstruction was removed through a small colon enterotomy using a paramedian approach. Information regarding signalment, history, clinical signs, surgical treatment, and follow-up status of the horse are described.  相似文献   

Evaluation of the healing of surgically created small colon serosal lesions in horses, treated by homologous pericardium implantation: An experimental study     

Maria Clia Ramos Bellenzani PhD  Raquel Yvonne Arantes Baccarin PhD  Cludio Henrique Carrara  Roberto Martins Manzan 《Journal of Equine Veterinary Science》2004,24(12):535-539

The healing of serosal lesions created in the small colon of horses and treated by homologous pericardium implantation was evaluated. A standing left flank laparotomy was performed in 6 horses. The small colon was partially exposed, and a defect measuring 2 × 2 cm was created in the serosa between the mesenteric border and the anti-mesenteric taenia. A square piece of homologous pericardium preserved in a 98% glycerin solution was rehydrated, dried, and sutured over the defect. Recovery of the animals was uneventful, with no signs of abdominal discomfort and no significant changes in physiologic variables, intestinal motility, behavior, appetite, or defecation pattern. The same was true for the erythrogram (packed cell volume, red cell count, and hemoglobin concentration), leukogram, and plasma fibrinogen values. The animals were reoperated on through the ventral midline approach after 4, 5, 6, 7, 8, and 35 weeks, respectively, and the implants were inspected visually and collected for microscopic study. No adhesions were found. Initially, there was a rim of fibrotic tissue covering the suture line, which regressed over time and was no longer evident after 7 weeks. From that moment on, progressive integration of the implant to the intestinal serosa was observed. Histopathologic examination revealed a late healing process, with infiltration of mononuclear cells, fibroblasts, neovascularization, and mature collagen fibers, which increased in intensity up to 6 weeks and then progressively regressed. At 5 weeks, it was already difficult to distinguish between the implant and the intestinal serosa. Our conclusion was that homologous pericardium implants have good acceptance in intestinal serosal lesions in horses and offer a good perspective for clinical application.  相似文献   

综合放松训练对结肠癌围术期患者免疫功能的影响     

于淑坤 《吉林林学院学报》2014,(2):214-217

目的探讨综合放松训练对结肠癌围术期患者免疫功能的影响．方法依据纳入标准选取80例接受手术治疗的患者作为研究样本,采用随机抽样的方法,分成对照组和实验组进行研究．结果两组患者术前第2天、术后第15天CD3＋,CD4＋,CD8＋和NK细胞水平比较差异无统计学意义．结论综合放松训练对结肠癌围术期患者免疫功能无明显影响．  相似文献