水牛初乳粉对新生仔猪肠道黏膜和肝脏IGF-I及IGF-IR基因表达的影响     

陈瑞芳  耿丹  郭傲民  张海燕  王士长 《山西农业大学学报(自然科学版)》2013,(6):526-530,552

水牛初乳富含免疫球蛋白和生长因子等活性物质,对新生哺乳动物具有良好的营养作用.选用新生仔猪为试验对象,分别饲喂水牛初乳粉（SCR组）、水牛常乳粉（SC组）、葡萄糖盐水（PY组）,并以0日龄新生仔猪作为对照（XD组）.研究水牛初乳粉对新生仔猪肠道粘膜及肝脏IGF-I、IGF-IR表达量的影响.结果表明,水牛初乳粉使新生仔猪在最初发育的3d内,肠道粘膜和肝脏中IGF-I基因表达量大幅度增加,IGF-I mRNA/β-actin比值高于XD组且P＜0.01;通过IGF-IR基因表达的研究发现,水牛初乳粉能增加肠粘膜中IGF-IR的表达量,但增幅小于IGF-I表达量;在肝脏中,水牛初乳粉使IGF-IR基因表达量略有降低,但与其它两个试验组相比,降低值最小,PY组次之,SC组降低值最大.水牛初乳能显著促进新生仔猪肠道和肝脏IGF-I基因表达,而对IGF-IR基因表达的影响表现为肠粘膜中表达量增加,脏脏中表达量降低.  相似文献   

舍饲绵羊产后56 d内乳成分变化规律研究     

马友记  董琪利 《畜牧兽医杂志》2013,(6):19-22,25

随着人民生活水平提高和膳食结构调整,绵羊奶必将受到人们的青睐.选取甘肃肉用绵羊新品种群、无角陶赛特和特克塞尔3个品种各8只母羊,对产后1～56 d泌乳理化性质变化进行研究,结果表明：3个品种乳成分及物理性质在第1～3 d变化最为明显,其中乳蛋白含量、非脂固形物含量和酸度3项指标呈现出前期快速下降,中期下降减缓,后期缓慢上升;乳糖指标则呈现出缓慢的前期上升,后期下降的趋势,对选取的3个品种初乳中蛋白、乳脂和可溶性固形物含量等指标对比,依次下降,乳糖含量和密度特克塞尔最高.常乳中除密度外3个品种间差异不显著（P＞0.05）.这为绵羊早期断奶和补饲提供了基础数据.  相似文献   

A Low-molecular-weight Fraction of Bovine Colostrum and Milk Enhances the Oxidative Burst Activity of Polymorphonuclear Leukocytes     

Sugisawa H  Itou T  Saito M  Moritomo T  Miura Y  Sakai T 《Veterinary research communications》2003,27(6):453-461

Bovine colostrum and milk contain many immunomodulatory components. The low-molecular-weight fraction (<10 kDa) was separated from colostrum and milk by gel filtration chromatography, and its effect on the oxidative burst of bovine polymorphonuclear leukocytes (PMNL) was investigated in vitro. The oxidative burst activity induced by Staphylococcus aureus was considerably enhanced when PMNLs were incubated with this low-molecular-weight fraction. However, phorbol 12-myristate 13-acetate did not trigger a burst after priming with this fraction. The oxidative burst activity enhanced by this fraction was reduced after heating. These results confirmed that a low-molecular-weight substance(s) of less than 10 kDa, present in bovine milk and colostrum, enhances the oxidative burst activity of PMNL.  相似文献   

The effect of dietary spray-dried bovine colostrum and plasma on the response of pigs to enterotoxigenic E. coli challenge after weaning     

M.R. King  T.J. Wester  P.C.H. Morel 《Livestock Science》2007,108(1-3):292-294

Twenty four, 21-d-old female pigs were fed diets containing either skim milk powder (CON+), spray-dried bovine colostrum (7.5%, BC+) or spray-dried bovine plasma (7.5%, BP+) and were dosed orally with 1 × 10⁹ CFU of E. coli O149:K88. Another group of 8 unchallenged pigs was fed the skim milk powder diet (CON−). On d 19 of the experiment all piglets were euthanased. Adverse effects of the E. coli challenge were observed variously throughout the small intestine in pigs consuming either the BC+ or BP+ diets. In this experiment, similar responses to the E. coli challenge were observed in both plasma and colostrum fed pigs, which suggests that spray-dried bovine colostrum may be a potential alternative to spray-dried bovine plasma.  相似文献   

Growth performance of piglets during the first two weeks of lactation affects the development of the intestinal microbiota          下载免费PDF全文

B. Morissette  G. Talbot  C. Beaulieu  M. Lessard 《Journal of animal physiology and animal nutrition》2018,102(2):525-532

The aim of this study was to evaluate the effect of newborn piglet weight gain during the first 2 weeks of lactation on the luminal and mucosal microbiota of the ileum and colon. The microbiota from high‐weight‐gain (HWG) and low‐weight‐gain (LWG) 2‐week‐old piglets was characterized by amplicon length heterogeneity PCR (LH‐PCR) and compared using diversity indices and multivariate statistical analyses. At birth, LWG piglets weighted in average 0.26 kg less than HWG piglets (p = .002). The weight difference between LWG and HWG piglets increased with time and reached 2.1 kg after 16 days of lactation (p < .0001). Based on these growth performance differences, estimated colostrum and milk intake was greater in HWG than in LWG piglets (p < .0001). Analysis of the LH‐PCR data of the microbiota using non‐metric multidimensional scaling (NMS) and blocked multiresponse permutation procedure (MRBP) revealed that the microbiota of the HWG and LWG piglets tended to differ in ileal mucosa (p = .097) and differed in colonic lumen (p = .024). The microbiota of HWG piglets had higher levels of Bacteroidetes, Bacteroides and Ruminoccocaceae, and lower proportions of Actinobacillus porcinus and Lactobacillus amylovorus when compared with those of LWG piglets. As the weight gain of nursing piglets is highly correlated with the amount of ingested colostrum and milk, the results strongly suggest that colostrum and milk intake in the first 2 weeks of life influenced the development of the gut microbiota.  相似文献   

Identification of sialyl oligosaccharides including an oligosaccharide nucleotide in colostrum of an addax (Addax nasomaculatus) (Subfamily Antelopinae)          下载免费PDF全文

Khuukhenbaatar Ganzorig  Takuya Asakawa  Masashi Sasaki  Tadao Saito  Isao Suzuki  Kenji Fukuda  Tadasu Urashima 《Animal Science Journal》2018,89(1):167-175

Mammalian milk/colostrum usually contains milk oligosaccharides along with the predominant lactose. Although milk oligosaccharides of a variety of Bovidae species including cow, sheep and goat have been characterized, those of the addax, an Antelopinae species of the Bovidae, have not as yet been clarified. In this study, several sialyl oligosaccharides were purified from a sample of addax colostrum and characterized as follows: Neu5Ac(α2‐8)Neu5Ac(α2‐3)Gal(β1‐4)Glc, Neu5Gc(α2‐8)Neu5Gc(α2‐3)Gal(β1‐4)Glc, Neu5Ac(α2‐3)Gal(β1‐4)Glc, Neu5Ac(α2‐6)Gal(β1‐4)GlcNAc, Neu5Gc(α2‐3)Gal(β1‐4)Glc, Neu5Gc(α2‐6)Gal(β1‐4)Glc, Neu5Gc(α2‐6)Gal(β1‐4)GlcNAc. In addition, an oligosaccharide nucleotide Neu5Gc(α2‐6)Gal(β1‐4)GlcNAcα1‐UDP was characterized. Molecular species of a variety of sialyl oligosaccharides found in milk and colostrum of these Bovidae were compared.  相似文献   

Oral ingestion of colostrum alters intestinal transforming growth factor-beta receptor intensity in newborn pigs   总被引：2，自引：0，他引：2  

Jie Mei  Yuanqiang Zhang  Tian Wang  Per T. Sangild  Ruo-Jun Xu   《Livestock Science》2006,105(1-3):214-222

Mammary gland secretion contains numerous bioactive compounds including transforming growth factor-beta (TGF-β). The concentrations of such bioactive compounds are usually much higher in colostrum compared with those in mature milk. To investigate possible effects of colostrum-borne TGF-β on the suckling animal, newborn piglets were naturally suckled or bottle-fed with porcine colostrum, bovine colostrum, porcine milk, infant formula or water for 24 h and intestinal TGF-β receptor intensity was assessed using an immunohistochemical staining technique in combination with computerized image analysis. The intestinal structure was also analyzed by morphometric analysis technique. It was observed that newborn pigs naturally suckled or bottle-fed with porcine or bovine colostrum had significantly greater intestinal villous height and crypt depth when compared with those fed with porcine milk, infant formula or water (p < 0.05). The immunostaining intensity for TGF-β receptors in the intestinal epithelium, particularly on the apical membrane of the villous epithelium, was significantly lower in naturally suckled or colostrum fed piglets compared with that in piglets fed with milk, infant formula or water (p < 0.05). Such decline in receptor intensity is likely the result of receptor internalization and degradation following exposure to colostrum-borne TGF-β. These findings suggest that colostrum-borne TGF-β can modulate intestinal TGF-β signalling pathways and may play a role in postnatal adaptation of the gut in newborn pigs.  相似文献   

Changes of vitamin E status of periparturient dairy cows and newborn calves     

Hajime KUMAGAI  Yot CHAIPAN 《Animal Science Journal》2004,75(6):541-547

Eleven primiparous Holstein Friesian and their crossbred calves (F1, Japanese Black cattle × Holstein Friesian) and 10 multiparous Holstein Friesian and their Holstein Friesian calves were used to evaluate vitamin E status in periparturient period. Plasma α‐tocopherol (α‐toc) concentrations of the multiparous cows were significantly higher than those of the primiparous cows from 60 days before expected calving to 90 days of lactation (P < 0.05). The multiparous cows had a further decrease in the concentrations of α‐toc and total lipid in plasma to the calving than the primiparous cows. Colostrum α‐toc concentrations in multiparous cows were significantly higher than those of the primiparous cows (P < 0.05). Plasma α‐toc concentrations of calves borne by the multiparous cows were significantly higher than those of the primiparous cows at 5 days of age (P < 0.05). Plasma α‐toc concentrations of calves were highest at 5 and 15 days of age in the calves borne by the multiparous and primiparous cows, respectively, and decreased thereafter till 90 days of age. The higher vitamin E status of multiparous cows over primiparous cows might have reflected nutritional composition in the rations. Their calves afforded higher plasma α‐toc levels after birth because of more α‐toc transfer via placenta and more α‐toc secretion in the colostrums thereafter. Plasma α‐toc concentrations of the calves might have decreased as the calves became dependent upon the solid feed of low vitamin E content.  相似文献   

牛初乳粉预防大鼠高血糖的实验研究   总被引：1，自引：0，他引：1  

丁红  张月明  陆东林  张丹凤  牛霞  王颢  吐尔逊江 《新疆畜牧业》2004,(2):11-14

目的:探讨牛初乳粉对大鼠糖尿病的预防作用。 方法:给大鼠灌胃3个不同剂量的牛初乳粉(BCP)溶液15d后腹腔注射链脲霉素(STZ)55mg/kg。注射后第7、14天测定各项指标(平均饮水量在注射后第6、13天测量)。血糖用Roche公司血糖仪测定,血脂用Beckman公司全自动生化分析仪测定,肝肾组织SOD、GSH-Px、NOS及MDA均用南京建成生物工程研究所相应试剂盒测定。结果:注射STZ后第7、14天中剂量BCP组的血糖均显著低于STZ组(P<0.05,P<0.01)。第13天中剂量组的平均饮水量的数值小于STZ组。第14天BCP各组的体重、胸腺指数与STZ组相比差异均无显著性,5个组之间的血脂、肝MDA、肝肾SOD、GSH-Px、NOS差异均无显著性。 结论:预防性给予BCP可预防STZ所致的高血糖,并使STZ大鼠平均饮水量减少(中剂量);但不能使STZ大鼠降低了的体重及胸腺指数恢复。  相似文献   

Effects of dexamethasone on mRNA abundance of nuclear receptors and hepatic nuclear receptor target genes in neonatal calves     

Greger DL  Blum JW 《Journal of animal physiology and animal nutrition》2007,91(1-2):62-67

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