Acute Escherichia coli Mastitis in Dairy Cattle: Diagnostic Parameters Associated with Poor Prognosis     

Seiichi HAGIWARA  Kouichiro MORI  Hiroyuki OKADA  Shin OIKAWA  Hajime NAGAHATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1431-1436

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伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立     

皮志媛  王一明  张海兰  雷程红 《安徽农业科学》2014,(2):480-482

[目的]建立一种快速检测李氏杆菌病的方法。[方法]通过对实验室保存的5株李氏杆菌分离株进行药敏试验,用甲醛灭活苗免疫试验动物,制备特异性抗体,将特异性抗体与抗原进行平板凝集试验。[结果]分离菌株对氨苄西林、庆大霉素、链霉素高度敏感,对头孢唑啉、左氧氟沙星和多粘菌素中度敏感,对头孢他啶、头孢呋辛钠耐药性较强。通过用甲醛灭活苗免疫试验动物来制备特异性抗体,将特异性抗体与抗原做平板凝集试验,建立一种快速准确检测李氏杆菌病的方法。[结论]成功建立了一种快速检测李氏杆菌病的方法。  相似文献   

猪附红细胞体病鉴别诊断研究进展   总被引：1，自引：0，他引：1  

詹海杰  侯志宏  王国艳  刘文俊 《山西农业科学》2014,(1):97-99

猪附红细胞体病是由立克次氏体目中的附红细胞体寄生于猪的血浆和红细胞中而引起的一种人畜共患的热性传染病。详细叙述了猪附红细胞体病的传播途径和鉴别诊断方法,旨在为控制该病的发生和流行提供一定参考。  相似文献   

Estimation of the repeatability and reproducibility of three diagnostic tests for infectious salmon anaemia virus     

Nérette P  Dohoo I  Hammell L  Gagné N  Barbash P  Maclean S  Yason C 《Journal of fish diseases》2005,28(2):101-110

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Assessment of enzyme linked immunosorbent assay based diagnostic kits (Toxokit-G and Toxokit-M) for the detection of IgG and IgM antibodies to Toxoplasma gondii in human serum     

G.M. Bhopale  S.R. Naik  G.G. Bhave  S.S. Naik  A. Gogate 《Comparative immunology, microbiology and infectious diseases》1997,20(4):309-314

An enzyme linked immunosorbent assay (ELISA) using penicillinase was developed in the form of diagnostic kits (Toxokit-G and Toxokit-M) for the detection of IgG and IgM antibodies to Toxoplasma gondii. The performance of both the kits was compared with commercially available diagnostic kits, i.e. Enzygnost-Toxoplasmosis/IgG (Behring Co., Germany), TOXOTEK-G (Flow Lab., U.K.) and Toxoplasma IgM Microassay (Diamedix Corp., U.S.A.) by testing toxoplasma-suspected human serum samples. The results indicate a good reliability between these diagnostic kits. Toxokit-G has 86.66 and 96.05% sensitivity and specificity respectively. The main advantage of Toxokit-G is that the end result can be assessed visually without using sophisticated instruments. Toxokit-M has 100% sensitivity and specificity and test results were not affected by the presence of antitoxoplasma IgG antibodies, rheumatoid factor or antinuclear antibodies.  相似文献   

DRIS norms and limiting nutrients in banana cultivation in the South of Ecuador     

Diego Villaseñor  Renato de Mello Prado  Gilmara Pereira da Silva  Manuel Carrillo  Wuellins Durango 《Journal of plant nutrition》2020,43(18):2785-2796

Abstract

Foliar analysis is an effective method to diagnose the nutritional status of plants. However, the mineral concentration in foliar tissue has traditionally been evaluated by assessing the activity of each element, without considering the interactions between them. To address this, dual interactions were calculated using the Diagnosis and Recommendation Integrated System (DRIS) to identify which crop nutrients are most influential in nutrient imbalances and which are the most limiting nutrients for the nutritional status of banana crops in Ecuador. To achieve this, a regional survey of the nutritional status and its productivity levels was conducted for 188 different sites during the crop season in 2017–2018, involving banana cultivars ‘Vallery’ and ‘Williams’, from the Cavendish subgroup. The DRIS calculation method was combined with Beaufils and Jones functions. From the initial 188 foliar samples, 83 samples (representing 44% of the population) were considered to represent the high-yield reference population, with yields of 38–60 t ha^?1. The DRIS method defined the mean nutritional balance index, which was not found to be statistically correlated (p?>?0.05) with productivity, revealing that there was no significant association with the nutritional status of the plants. Specific DRIS norms were obtained and indicated that deficiencies in K, N, Ca, and Fe, and excesses in Mn, B, Cl, Zn, S, Cu, and Mg were the most limiting nutrients for banana cultivars in the south of Ecuador.  相似文献   

土壤中施加锌对豌豆(Pisum sativum L.)中锌浓度以及其种子产量的影响     

E. RAFIQUE  M. YOUSRA  M. MAHMOOD-UL-HASSAN  S. SARWAR  T. TABASSAM  T. K. CHOUDHARY 《土壤圈》2015,25(2):275-281

A 2-year field experiment was conducted to assess the effect of applied zinc（Zn） on the seed yield of pea（Pisum sativum L.） and to determine the internal Zn requirement of pea with emphasis on the seed and leaves as index tissues.The experiment was carried out at two different locations（Talagang,Chakwal district and National Agricultural Research Centre（NARC）,Islamabad） in the Potohar Plateau,Pakistan by growing three pea cultivars（Green feast,Climax,and Meteor）.The soils were fertilized with 0,2,4,8,and 16 kg Zn ha^-1 along with recommended basal fertilization of nitrogen（N）,phosphorus（P）,potassium（K）,and boron（B）.Zinc application increased seed yield significantly for all the three cultivars.Maximum increase in the pea seed yield（2-year mean） was21%and 15%for Green feast,28%and 21%for Climax,and 34%and 26%for Meteor at Talagang and NARC,respectively.In all cultivars,Zn concentrations in leaves and seed increased to varying extents as a result of Zn application.Fertiliser Zn requirement for near-maximum seed yield varied from 3.2 to 5.3 kg ha^-1 for different cultivars.Zinc concentrations of leaves and seeds appeared to be a good indicator of soil Zn availability.The critical Zn concentration range sufficient for 95%maximum yield（internal Zn requirement）was 42-53 mg kg^-1 in the pea leaves and 45-60 mg kg^-1 in the seeds of the three pea cultivars studied.  相似文献   

大白菜氮素营养的反射仪法快速诊断及应用     

高艳明  曹云娥  孙权 《西北农业学报》2007,16(1):140-144

应用Merck RQflex 2型反射仪和硝酸根试纸条研究了氮肥施用数量和施用方式对宁夏银川平原复种大白菜产量和品质的影响,并探讨了大白菜硝酸盐与氮肥用量的相关性和速测指标。结果表明,大白菜的产量随施肥量的增加而增加,表现出线性——平台关系;施氮肥提高了大白菜产量和净菜率;关键需肥期进行追肥比传统的基肥加追肥具有显著的增产效果,且收获时体内硝酸盐水平降低;大白菜外叶叶柄硝酸盐含量随施氮量增加而显著增加,但随生育期延长而下降。复种大白菜最佳经济施氮量为572 kg/hm2,叶柄硝酸盐适宜含量为3 485.28 mg/kg。  相似文献   

Mg对乌龙岭龙眼苗期植株生长的影响及诊断指标     

李延  刘星辉  庄卫民 《福建农林大学学报(自然科学版)》2001,30(3):394-397

水培试验结果表明 ,Mg对龙眼苗期生长有明显的影响 .缺 Mg胁迫下 ,龙眼生物量、根干重、根体积、根总吸收面积和根系活力降低 ,根 /冠比值下降说明缺 Mg对龙眼根系生长的影响大于地上部 .苗期龙眼 Mg素营养诊断的适宜部位为复叶下部小叶 ,诊断指标 :临界值是 2 .5 g· kg-1,适宜范围为 2 .5 - 4 .0 g· kg-1,>4 .0g· kg-1为过量  相似文献   

湖北省地带性土壤发生学特性的探讨     

王庆云  徐凤琳  刘自清 《华中农业大学学报》1988,(4)

研究了湖北省不同气候带、不同母质上发育的棕红壤、黄棕壤和黄褐土的主要性状。结果表明,某些性状如土体的Sa、ba值和粘粒的活性等,其风化和淋溶强度虽都有自北而南增强的趋势,但有重叠现象,可作参考性分类依据;土壤饱和度、粘粒的交换量、酸度和粘粒的矿物学组成等在各土类之间有较好的分异性,可作为本地区土壤分类的诊断特性。  相似文献