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71.
72.
采用自体器官移植的思路,探索用自体部分带蒂空肠代替尿道的手术方法,以解决实际中动物因尿道结石、尿道损伤等所致的自身尿道无法正常排泄尿液的情况。经过术后观察,犬的各项生理指标均趋于正常。结果表明,此段带蒂空肠可存活,并可代替尿道,保证机体正常的尿液排泄,为犬的尿道再造术提供了一种新的方法。 相似文献
73.
AIM: To investigate the effect of rosiglitazone, a peroxisome proliferators-activated receptor γ(PPARγ) agonist, on the expression of PPARγ, the activation of NF-κB and intestine injury in the rats undergoing orthotopic autologous liver transplantation(OALT).METHODS: Sprague-Dawley male rats were randomly divided into 4 groups:control group, sham group, OALT group and rosiglitazone(0.3 mg/kg, iv) pretreatment(ROS+OALT) group. The OALT model was established, and the intestinal tissues were collected 8 h after the liver reperfusion. The intestinal tissue sections were stained to visualize the damage. The expression of PPARγ and NF-κB in the tissues, the concentrations of diamine oxidase(DAO) and fatty acid-binding protein 2(FABP2) in the serum and the concentration of TNF-α and IL-6 in the tissues were measured.RESULTS: Compared with sham group, the intestinal mucosa of the rats showed obvious pathological injury after liver reperfusion in OALT group and ROS group, the Chiu,s scores of intestinal mucosa was significantly higher, and the serum concentrations of DAO and FABP2 increased(P<0.05). After rosiglitazone pretreatment, the injury of intestinal mucosa of the rats was alleviated, the Chiu,s scores was lower and the serum concentrations of DAO and FABP2 decreased(P<0.05), the PPARγ expression was obviously up-regulated in the intestinal tissues, the nuclear translocation of NF-κB was reduced and the concentrations of IL-6 and TNF-α were decreased.CONCLUSION: During perioperative period of OALT in rats, the inflammatory responses are obvious. Furthermore, obvious intestinal injury occurs. PPARγ agonist rosiglitazone obviously up-regulates PPARγ expression and inhibits the inflammation in the intestines, thus protecting against intestinal injury in rats undergoing OALT. 相似文献
74.
Summary The effect of direct vernalization of immature embryos on flowering was studied in six winter wheat genotypes. Fourteen-, 17-, and 20-day-old embryos were excised and vernalized for 0–6 weeks on synthetic medium during a conditioning period. Percent germination of embryos was high (overall 96.1%), and free from genotypic effects. Genotypes differed for flowering in response to cold treatment of excised embryos. Embryo vernalization was as effective as or more than conventional vernalization (control, seedling vernalization for 6 weeks). Seventeen-day-old embryos were the most responsive to vernalization. With a 5-week vernalization of 17-day-old embryos, the percentage of plants anthesed was higher than those from 14-and 20-day-old embryos. For 17-day-old embryos vernalized for 5 weeks, the mean number of days from culture to anthesis was less than that of 6 week vernalization, less than that of 14- and 20-day-old embryos, and less than controls.Purdue Univ., Agronomy Dept., W. Lafayette, IN 47907, USA. 相似文献
75.
Summary A method is described for selecting resistant transformed tomato genotypes in vitro at the stage of immature embryos. The utilization of HLH nutrient medium with the selective agent kanamycin is proposed. Normal development of seedlings from immature embryos, which do not form callus, is a good and true indicator for isolation of resistant genotypes. The immature embryos do not germinate and develop on MS selective medium. 相似文献
76.
研究了国产激素(FSH)在使用方法、时间、剂量、配制比例等方面对奶牛超数排卵效果的影响,并对供体牛的年龄、胎次、产后日期,重复超排及季节、气候等各种因素在超数排卵效果方面进行了探讨。研究结果还表明国产FSH超排效果比较稳定。 相似文献
77.
四种鸢尾属植物种子休眠和萌发研究 总被引:4,自引:1,他引:4
燕子花(Iris laeviga ta)、玉蝉花(Iris ensa ta)、溪荪(Iris sanguinea)、黄菖蒲(Iris pseudacorus)是鸢尾科4种喜水湿植物。经萌发实验证明,除溪荪种子没有休眠外,其它3种种子都有休眠。经去除种皮、低温沙藏、赤霉素处理和胚培养4种处理,证明燕子花、玉蝉花、黄菖蒲中抑制种子萌发的主要因素是胚乳。不同种打破休眠的有效方法不同,其中50 mg/L GA3处理可提高黄菖蒲种子的发芽率,达到84.4%,而低温沙藏30 d可使玉蝉花种子的发芽率达到94.4%,低温沙藏90 d则可提高燕子花的发芽率达到87.8%。 相似文献
78.
运用正交试验的方法研究炼苗时间、不同遮阳网遮光率以及不同基质对香樟试管苗移植效果的影响,结果表明:炼苗时间7 d,遮阳网遮光率60%,基质为木屑∶泥炭土(1∶1)对于香樟试管苗移植效果最佳。 相似文献
79.
本研究应用国产激素和器械对奶牛进行超数排卵,采用胚胎移植技术使黄牛繁殖奶牛。5a共超排供体牛185头,成功159头,成功率为81.95%;共获卵1201枚,头均7.55±6.37枚,其中可用胚胎880枚,头均5.68±4.96枚。同期发情处理受体牛236头,发情139头,发情率58.90%;鲜胚移植受体牛178头,受胎78头,受胎率为43,82%,其中移植受体黄牛145头,受胎61头,受胎率42.07%,移植受体奶牛33头,受胎17头,受胎率为51.52%;冷冻胚胎移植受体牛61头,受胎27头,受胎率为37.70%;53胚胎移植产犊85头。确立了应用国产激素垂体促卵泡素(FSH)和前列腺素(PGG_(22))诱发牛超数排卵和发情同期化处理的最佳程序和剂量,并总结出了采卵方法、胚胎鉴定、胚胎冷冻保存和移植等相应环节的技术规程。 相似文献
80.
新鲜羊膜移植术治疗眼表烧伤晚期并发症的临床研究 总被引:1,自引:0,他引:1
目的:研究新鲜羊膜移植术治疗眼表烧伤晚期并发症的可行性、手术方法和临床效果。方法:眼表烧伤晚期病例18例(18眼),其中角膜血管翳8例,角膜溃疡7例,睑球粘连3例,采用新鲜羊膜移植治疗。手术方法:单层与多层羊膜移植或联合眼睑成形,角膜缘干细胞移植术。术后随访6~12个月。结果:18例术后植片均未见急性排斥反应,术后4~6周稳定愈合;13眼视力有不同程度提高。18眼中有17眼治愈。结论:新鲜羊膜是治疗眼表烧伤晚期并发症的有效方法。 相似文献