Comparison of cholesterol levels among lipoprotein fractions separated by anion-exchange high-performance liquid chromatography in periparturient Holstein–Friesian dairy cows     

Tomoya TAKAHASHI  Akihiro MORI  Hitomi ODA  Isao MURAYAMA  Mitsuhiko KOUNO  Toshinori SAKO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):260

Changes in lipoprotein profiles occur in dairy cows during the periparturient period and in cows with transition cow disease. Here, the lipoprotein profiles of Holstein–Friesian dairy cows during the periparturient period were obtained by anion-exchange, high-performance liquid chromatography to evaluate the usefulness of lipoprotein profile evaluation during the periparturient period and in cows with fatty liver and milk fever. Lipoprotein levels (including total and high- (HDL-C) and low-density lipoprotein (LDL-C) cholesterol) in 10 healthy cows were low 4 weeks prepartum, with the lowest values at calving or within 1 week of calving; the values increased at 8 weeks postpartum. The lipoprotein levels were measured in 16 cows diagnosed with fatty liver (n=10) or milk fever (n=6) and compared to 10 healthy dairy cows. A significant difference was observed in HDL-C between healthy cows (at calving and 1 week postpartum), and the fatty liver and milk fever cows. Cows with fatty liver and milk fever had a lower mean HDL-C than the 10 healthy dairy cows at calving and 1 week postpartum. HDL-C might be a good indicator of energy balance for differentiating healthy cows from those with transition cow disease.  相似文献   

大型贯流式泵站快速门加速启动过渡过程     

许哲  郑源  阚阚  陈会向  陆洋  戴景 《排灌机械工程学报》2020,38(10):1016-1022

为研究大型贯流式泵站机组闸门加速启动过程中的外特性参数变化及流态变化,对灯泡贯流泵机组进行三维建模,采用Fluent软件中的UDF来控制闸门的启动过程,使用铺层法动网格技术来控制闸门处网格生成与消灭,并设定S-A模型作为湍流模型,对灯泡贯流泵机组启动过程进行瞬态数值模拟.计算结果表明:当贯流泵机组快速门以10倍设计速度启动时,机组的转速与流量将以更快的速度达到额定转速与额定流量,同时瞬间最大倒灌流量相比未加速启动时增加了30%.当贯流泵机组处于倒流状态时,靠近轮毂处叶轮叶道压力面的压强呈现出从叶轮进水边向出水边逐渐递增的分布规律.当机组处于零流量的临界状态时,混乱的流态导致压力集中及脱流现象加重,叶轮进水边负压区范围扩大且程度加深.  相似文献   

Dopamine Antagonist Affects Luteal Function But Not Cyclicity During the Autumn Transition   总被引：1，自引：1，他引：0  

S.S. King PhD  J.F. Roser PhD  D.L. Cross PhD  K.L. Jones PhD 《Journal of Equine Veterinary Science》2008,28(6):345-350

The autumn transition is characterized by a number of anomalies affecting the luteal phase of the estrous cycle, such as declining progesterone secretion from the corpus luteum and increased rate of failure of luteolysis. Prolactin also decreases. We theorized that these events may be linked, possibly through dopamine. We administered domperidone, a specific dopamine D2 receptor antagonist from September 12 to anestrus or January 15, either daily (n = 8), or once per day for the first 7 days of each month (n = 7). Controls (n = 7) received no treatment. Mean progesterone and prolactin concentrations were compared with summer cycles. Time to entry into anestrus and incidence of spontaneously prolonged luteal activity was compared between groups during the autumn transition. Prolactin declined from June through October equally in all groups. There was no difference between groups in time to anestrus. Progesterone decline was prevented (daily treatment) or delayed for a prolonged period (weekly group) in autumn. The incidence of spontaneously prolonged corpus luteum activity was reduced to summer levels in both domperidone groups compared with the control. We concluded that autumn prolactin decline was not driven through dopaminergic D2 receptor inhibition of pituitary lactotrophs. Sustained progesterone synthesis through the autumn may be the result of action of the D2 receptor antagonist with dopamine receptors on the corpus luteum. Autumnal luteolytic function appears to have a dopamine-influenced component. There does not appear to be a causative relationship between autumnal progesterone and prolactin secretion.  相似文献   

Calreticulin promotes migration and invasion of nasopharyngeal carcinoma cells by inducing cell EMT     

ZHU Jun-hui  JIN Ming  QIU Hao  ZHANG He-liang  MA Wei  XIAO Xuan  LI Chen-song  LUO Zhao-yang  ZHANG Zhi-wei 《园艺学报》2018,34(5):925-929

AIM:To observe the expression of calreticulin (CRT) in nasopharyngeal carcinoma tissues, analyze the significance of clinical pathology and the influence on epithelial-mesencymal transition (EMT) of CNE2 cells. METHODS:The expression of calreticulin was detected by immunohistochemistry in 52 nasopharyngeal carcinoma and 57 nasopharyngeal benign tissues, and the significance of clinical pathology was evaluated. The calreticulin gene-specific small interfering RNA was constructed, and then was transfected into the NPC cell line CNE2 using the cationic liposome method. The effect of CRT on the morphological changes of the CNE2 cells was observed under light microscope. The effect of CRT on the cell migration and invasion abilities of the CNE2 cells was detected by Transwell migration and invasion assays. The expression of EMT-related proteins E-cadherin, vimentin, transforming growth factor (TGF)-β and matrix metalloproteinase (MMP)-9 in the CNE2 cells was determined by Western blot. RESULTS:The positive expression rate of CRT in the benign lesion tissues was 19.29% (11/57), which was significantly increased in the nasopharyngeal carcinoma tissues as 82.69% (43/52). The expression rate of CRT was positively correlated with the stage of nasopharyngeal carcinoma and lymph node metastasis (P<0.05). Knockdown of CRT expression made the CNE2 cells showing a spindle shape to a flat, cobblestone-like epithelial state change, arranged more compact, and the migration and invasion abilities were significantly decreased (P<0.05). Knockdown of CRT expression resulted in significant increase in the protein expression of E-cadhe-rin, and the decreases in the protein expression of vimentin, TGF-β and MMP-9 in the CNE2 cells (P<0.05). CONCLUSION:Calreticulin expression in nasopharyngeal carcinoma is significantly higher and positively correlated with nasopharyngeal carcinoma stage and lymph node metastasis. Calreticulin promotes cell migration and invasion of nasopharyngeal carcinoma CNE2 cells by inducing EMT.  相似文献   

IL-9 induces epithelial-mesenchymal transition of gastric cancer MKN-45 cells by TGF-β/Smad signaling pathway     

RUAN Shui-liang  HAN Chen-yang  GUAN Qiao-bin  YANG Yi 《园艺学报》2018,34(10):1748-1753

  相似文献   

Juglone inhibits epithelial-mesenchymal transition of prostate cancer cells by regulating Wnt/β-catenin/Snail signaling pathway     

ZHAO Liang-zhong  CUI Jia-bo  MA Jing  WANG Li-guo  FANG Fang 《园艺学报》2018,34(10):1910

AIM: To investigate the mechanism of juglone on epithelial-mesenchymal transition in prostate cancer cells. METHODS: Human prostate cancer LNCaP cells were divided into control group (without juglone), 12.5 μmol/L juglone group and 25 μmol/L juglone group. LNCaP cells in the latter 2 groups were treated with juglone for 24 h. The invasion ability of the LNCaP cells was detected by Transwell assay. The protein expression of E-cadherin, vimentin, Snail and β-catenin was determined by Western blot. The LNCaP cells were treated with LiCl and juglone in combination for 24 h, and the protein expression of Snail and E-cadherin was detected by Western blot.RESULTS: The results of Trans-well invasion assay showed that the invasion ability in juglone groups was significantly decreased (P<0.01). The protein expression of E-cadherin in the LNCaP cells treated with juglone was increased, and the expression levels of vimentin and β-catenin were reduced (P<0.01). Treatment with LiCl significantly attenuated the inhibitory effect of juglone on Snail expression and subsequent down-regulation of E-cadherin expression. CONCLUSION: Juglone inhibits the epithelial-mesenchymal transition by inhibiting the Wnt/β-catenin/Snail signaling pathway in the LNCaP cells.  相似文献   

Over-expression of SCUBE2 suppresses epithelial-mesenchymal transition through Wnt/β-catenin signaling pathway in colorectal cancer cells     

WANG Li  ZHANG Ning  ZHANG Long-jiang  XI Zuo-wu 《园艺学报》2016,32(12):2245-2250

AIM: To study the effect of SCUBE2 on epithelial-mesenchymal transition (EMT) in colorectal cancer cells and its mechanism. METHODS: The expression of SCUBE2 in human colorectal cancer cell line HCT116 and normal colonic cell line FHC was detected by real-time PCR and Western blot. HCT116 cells were transfected with GV144-SCUBE2 to over-express SCUBE2, and then the cell viability, migration, and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively. The expression of EMT markers (E-cadherin, vimentin, and Snail), β-catenin, c-Myc and cyclin D1 in the HCT116 cells was analyzed by real-time PCR or Western blot after transfection with GV144-SCUBE2 for 6 h, followed by the stimulation of 10 μg/L recombinant TGF-β1 protein for 48 h. Additionally, the EMT process of HCT116 cells, which were stimulated by TGF-β1, over-expressed SCUBE2, and treated with Wnt/β-catenin pathway activator lithium chloride (LiCl) or inhibitor XAV93920, was analyzed by Western blot. RESULTS: Compared with FHC cells, the expression of SCUBE2 in the HCT116 cells was significantly decreased. The viability and migration ability of the HCT116 cells were suppressed by SCUBE2 over-expression, but the apoptosis was not markedly changed. Elevated expression of SCUBE2 increased E-cadherin expression, and decreased the expression of vimentin, Snail, β-catenin, c-Myc and cyclin D1 induced by TGF-β1. Treatment with LiCl blocked but treatment with XAV93920 enhanced the effects of SCUBE2 on EMT. CONCLUSION: Over-expression of SCUBE2 may inhibit the cell growth and migration, and suppress EMT through Wnt/β-catenin signaling pathway.  相似文献   

玻璃化转变温度对层压板性能的影响     

刘涛 《浙江林学院学报》1999,16(3):297-302

探求层压板的玻璃化转变温度（ｔｇ）对层压板性能的影响,对研制开发层压板新产品和选择适用层压板制作印刷电路板有重要的理论意义和实际意义。对不同ｔｇ的层压板的耐热性、抗弯强度和热膨胀性等８项性能进行了比较。结果表明层压板的ｔｇ升高,层压板的机械和电气性能明显提高。  相似文献   

中国粮食主产区耕地绿色低碳利用转型指数构建及分区     

符海月  吴树东  姜朋辉 《农业工程学报》2023,39(23):238-246

探究耕地绿色低碳转型方法与对策对于解决耕地环境本底问题,助推农业可持续发展,确保国家粮食安全、生态安全以及“双碳”目标的实现具有重要意义。该研究建立耕地绿色低碳利用投入-产出分析模型,基于“现状-目标”对比分析,构建耕地绿色低碳利用转型指数,划分耕地绿色低碳利用转型管控区,提出相应管控路径,并以粮食主产区171个地级市为对象进行实证研究。研究结果表明：1)2000—2020年间,粮食主产区耕地绿色低碳利用水平趋升,呈北高南低态势;2）预测2030年耕地绿色低碳水平会有所提高,但区域间增幅差异明显;3）降污减碳严控区、管控区、防护区和防污固碳保护区各占粮食主产区的9.36%、39.77%、10.52%和40.35%,主要分布在南部、中南部、山东省烟台市及东部沿海、内蒙古以及四川省等市;结合各区社会经济条件,提出相应强化耕地生产要素高效配置、创新耕地固碳减排技术、加强落实生产绿色低碳实践及建立耕地碳汇系统的差别化绿色低碳转型导向建议。研究结果对于拓展耕地绿色低碳利用转型理论与方法研究具有一定借鉴意义,也可为探索耕地绿色低碳转型路径,提出差别化管控建议,推动耕地可持续利用和农业绿色转型提供决...  相似文献   

一种基于小波变换的图像过渡区提取及分割方法   总被引：8，自引：3，他引：5  

赵茂程  郑加强  凌小静 《农业工程学报》2005,21(11):103-107

具有复杂背景的树木图像的分割对于精确对靶施药及智能化植保机械的设计具有重要意义。为实现树木图像的精确分割,针对该类图像的特点,该文提出了一种基于小波变换的过渡区提取树木图像分割方法。通过对比小波变换系数、小波变换系数聚类以及小波包系数,最终选取了同时能够分解出更多高频、低频信息的小波包变换系数提取特征,根据小波包变换系数定义了小波能量比参数,将小波能量比参数值归一化为图像灰度值,采用自适应阈值和神经网络两种方法提取了过渡区,实现了具有复杂背景树木图像的分割。试验表明,该方法分割精度高,对于分割复杂背景的树木图像具有特别意义。  相似文献