Canine renal failure syndrome in three dogs     

Won-Il Jeong  Sun Hee Do  Da-Hee Jeong  Jae-Yong Chung  Hai-Jie Yang  Dong-Wei Yuan  Il-Hwa Hong  Jin-Kyu Park  Moon-Jung Goo  Kyu-Shik Jeong 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(3):299-301

Three dead dogs were brought to the College of Veterinary Medicine, Kyungpook National University for study. Clinically, all the dogs showed emaciation, anorexia, depression, hemorrhagic vomiting and diarrhea for 7~10 days before death. All the clinical signs were first noted for about one month after feeding the dogs with commercial diets. At necropsy, all 3 dogs had severe renal damage with the same green-yellowish colored nephroliths in the renal pelvis. They also showed systemic hemorrhage and calcification of several organs, which might have been induced by uremia. Microscopically, necrosis, calcification and calculi were detected in the renal tubules, and especially in the proximal convoluted tubules and collecting ducts of the kidney. These findings were supportive of a mycotoxic effect, and especially on their kidneys. However, the precise cause of the toxic effect in these cases of canine renal failure could not be determined.  相似文献   

果实特异启动子调控薄皮甜瓜ACC合成酶cDNA反义表达载体的构建     

郭庆勋  秦智伟  李景鹏 《果树学报》2006,23(3):411-414

应用反义RNA技术抑制甜瓜成熟过程中内源乙烯的合成,从而培育耐贮运品种是解决甜瓜延熟保鲜难题的可行新方法。根据GenBank中甜瓜、黄瓜ACC合成酶基因氨基酸保守序列设计引物,从成熟的薄皮甜瓜(齐甜1号)果肉组织中提取总RNA,经RT-PCR扩增得到约0.7kb的ACC合成酶cDNA片段,将其克隆到质粒载体pGEM-TEasy中,测序表明,该基因为777bp,编码258个氨基酸;从番茄(东农706)叶片组织中提取总DNA,经PCR扩增得到约2.2kb的E8基因片段,将其克隆到质粒载体pGEM-TEasy中,测序表明,该基因为2192bp;以pCAM2301为起始植物表达载体,pCAM-GT为中间载体,成功构建了果实特异启动子(E8)调控薄皮甜瓜ACC合成酶cDNA反义表达载体,采用冻融法将其转入根癌农杆菌LBA4404,得到了完整的Ti质粒表达载体系统。  相似文献   

A Possible Correlation between the Blood Leukocyte Formula and the Use of Glucocorticoids as Growth Promoters in Beef Cattle     

Brambilla  G.  Fiori  M.  Pierdominici  E.  Antonucci  G.  Giorgi  P.  Ramazza  V.  Zucchi  M. 《Veterinary research communications》1998,22(7):457-465

Clinical analyses designed to set welfare parameters were performed on blood drawn from the caudal vein of 14 groups of cattle (young bulls and heifers) (n = 10) from 480 to 550 kg b.w., each group representative of a different farm. The leukocyte formula exhibited a lymphocytopenia in four groups compared with the values from a control group (n = 50). This finding was related to the possible illicit use of corticoids as growth promoters in meat production. The individual plasmas tested negative by two different ELISA kits for corticosteroids, but chemical analyses by LC-MS/APCI (detection limit 0.5 ng/ml) on the pooled plasma of each of the 14 groups revealed the presence of beclomethasone and fluocinolone acetonide in 3 of the 4 suspect farms. These corticosteroids are not always efficiently screened by commercially available immunoassays. The epidemiological reliability of blood analysis as a screening test for such drugs is discussed in the light of the need for quality certification of the whole meat production processes from farm to fork, and for enhanced animal welfare.  相似文献   

益生菌发酵乳无菌提取物对空肠弯曲杆菌flaA σ²⁸启动子的抑制作用（英文）     

丁武寇莉萍  王海风胡巅 《西北农林科技大学学报(自然科学版)》2007,35(10):173-177,182

为了研究益生菌发酵乳无菌提取物对食源性有害微生物(空肠弯曲杆菌)有毒基因表达的抑制作用,以利用空肠弯曲杆菌的有毒基因flaA σ28启动子和无启动子的质粒pRYluxCDABE联结构建的生物冷光转基因模型为试验材料,通过检测生物发光特性的方法,研究了空肠弯曲杆菌有毒基因flaA表达与益生菌(5种双岐杆菌,6种乳酸杆菌)发酵乳无菌提取物之间的关系。结果表明,益生菌发酵乳无菌提取物对空肠弯曲杆菌flaA σ28启动子的活性有显著的抑制作用(P<0.05),并可显著抑制空肠弯曲杆菌有害基因flaA的表达。  相似文献   

家蚕促产卵剂的再探讨   总被引：1，自引：0，他引：1  

陆雪芳  朱根生 《蚕桑通报》1997,(4):31-33

本文就促排三号、医用催产素对促进家蚕蛾产卵的效果及试用方法进行探讨。试验证明，前者可提高产出卵经约５％，但因品种而有差异。后者对临产蛾有加速速度的作用。  相似文献   

不同野生稻居群DREB1B启动子的克隆与进化分析     

徐国云  陈荣军  徐孟亮  崔延春 《湖北农业科学》2008,47(3):245-248

根据Genbank所公布OsDREBIB的启动子序列设计引物,从12个不同普通野生稻居群和2个主要栽培稻亚种(籼、粳)克隆DREBIB启动子序列.结果表明,该启动子绝大部分区域都非常保守,存在1个AGC重复序列,若含有5个AGC重复,重复序列后第16个碱基为A,若含有6个或7个AGC重复,此位点碱基为T,并且在启动子中发现几个逆境相关的顺式作用元件.进化树分析表明,同一地域分布的居群存在相近的进化关系,但也受海拔等因素的影响.  相似文献   

绵羊CCL19基因启动子活性分析及其转录调控元件筛选     

翟哲  陈思  吴艳茹  王雪梅  李崇瑞  刘志勇  陈巧玲  王凤阳  杜丽  李昌  金宁一 《中国畜牧兽医》2022,49(4):1213-1222

【目的】 鉴定绵羊趋化因子C-C基序配体19(C-C motif chemokine ligand 19,CCL19)基因启动子的核心启动子区域和关键转录因子,探究该基因在转录调控方面的作用机制。【方法】 选取绵羊CCL19基因5'-侧翼序列1 000 bp,PCR扩增启动子的7个不同长度的截短片段,并连接至pGL3-Basic质粒;将重组质粒与pRL-TK质粒共转染到293T细胞中,结合双荧光素酶报告基因检测系统分析不同截短片段的相对荧光活性。利用在线预测软件分析和筛选CCL19基因核心启动子区域内的转录因子结合位点。采用定点突变技术构建转录因子结合位点缺失的荧光素酶报告载体,与pRL-TK质粒共转染到293T细胞,分析转录因子结合位点缺失质粒的相对荧光活性。【结果】 成功构建了7个不同长度(pGL3-P、pGL3-P1、pGL3-P2、pGL3-P3、pGL3-P4、pGL3-P5及pGL3-P6)的CCL19基因启动子片段的荧光素酶报告载体;采用双荧光素酶报告基因检测系统鉴定出转录起始位点上游－256/－186 bp为CCL19基因启动子核心启动子区域,表明该区域对CCL19基因转录调控有重要作用。生物信息学分析预测到该区域存在POU5F1(-201/-189 bp)、ZBTB26(-228/-217 bp)、FOXI1(-239/-228 bp)、GLI2(-255/-243 bp)和SP2(-219/-211 bp) 5个转录因子的结合位点,并成功构建了转录因子结合位点缺失的荧光素酶报告载体。双荧光素酶报告基因检测系统分析显示,POU5F1转录因子的结合位点缺失后绵羊CCL19基因转录活性极显著降低(P<0.01),FOXI1、ZBTB26、SP2转录因子结合位点缺失后绵羊CCL19基因转录活性均极显著升高(P<0.01)。【结论】 试验成功构建CCL19基因启动子荧光素酶报告载体,确定CCL19基因启动子的核心启动子区域为转录起始位点上游－256/－186 bp,并鉴定出转录因子POU5F1结合位点可能是CCL19基因转录的重要调控位点,为下一步研究绵羊CCL19基因在先天性免疫、适应性免疫和淋巴细胞迁移等方面的功能提供理论基础。  相似文献   

Evaluation of Dutch elm disease susceptibility and pathogenicity of Ophiostoma ulmi using micropropagated elm shoots     

N. Dorion  C. Bigot  P. Neumann 《Forest Pathology》1994,24(2):112-122

Elm (Ulmus sp.) shoots from in vitro micropropagated plants were tested with Ophiostoma ulmi culture filtrates, at different dilutions to determine the susceptibility of different host clones to Dutch elm disease (DED). ‘Commelin’, a susceptible Dutch hybrid, showed a high wilting index but that of American elm was higher. Although U. pumila × japonica (Sapporo gold 2) was the most tolerant to spore inoculation, its wilting index was as high as that of ‘Commelin’. The absorption capacity and wilting index of the elm shoots showed consistent correlation to isolate effectiveness (as determined by inoculation experiments), to production of toxin (cerato-ulmin) and to filtrate dilution. This method could be used to assess the effectiveness of the different O. ulmi isolates and for the detection of elm genotypes that are highly susceptible to Dutch elm disease.  相似文献   

Isolation and analysis of a TIR-specific promoter from poplar   总被引：2，自引：0，他引：2  

Zheng Hui-quan Lin Shan-zhi Zhang Qian Zhang Zhen-zhen Zhang Zhi-yi Lei Yang Hou Lu 《中国林学(英文版)》2007,9(2):95-106

  相似文献   

大豆根特异性GmPR1-9启动子的鉴定及其在根腐病抗性中的应用     

闫强  薛冬  胡亚群  周琰琰  韦雅雯  袁星星  陈新 《中国农业科学》2022,55(20):3885-3896

【目的】 鉴定大豆根特异性启动子及其最小调控片段,并利用启动子工程技术构建时空特异人工启动子并评价其在根腐病抗性中的应用价值,为大豆抗疫霉根腐病的遗传改良提供遗传元件。【方法】 通过分析大豆根、茎和叶片转录组数据,筛选在根中特异高水平表达的基因,克隆获得其启动子序列。根据顺式元件的分布位置构建截短载体,并驱动GUS报告基因在大豆发状根组织中超表达,筛选控制根特异性表达的核心片段。将获得的核心启动子片段与疫霉菌诱导启动子元件p4XD串联构建人工启动子驱动疫霉抗性相关基因GmNDR1在大豆发状根中超表达,分析转基因组织对疫霉菌抗性水平及目的基因在病原菌侵染过程中的表达水平。利用转基因本氏烟草从整株水平评价转基因材料对疫霉菌的抗性水平。【结果】 通过筛选发现6个大豆根特异性表达的PR1同源基因,其中,pGmPR1-9具有最高的启动子表达活性。PLACE在线预测发现其启动子区域含有大量的根特异表达相关顺式元件。对pGmPR1-9启动子进行截短试验,发现5′端截短片段L1、L2、L3、L4和L5均具有启动GUS表达活性,长度为166 bp的L5（-166—-1）片段具有全长启动子80%的活性,并可驱动GUS在转基因烟草根中特异表达;3个3′端截短片段R1、R2、R3和1个双端截短片段M1几乎检测不到GUS酶活性。p4XD-L5融合片段驱动GmNDR1在大豆发状根中超表达后可显著提高大豆发状根对疫霉菌的抗性,超表达发状根接种病原菌后发病程度和病斑长度显著低于对照,疫霉菌丝积累量在接种48 h时减少66.5%。GmNDR1在超表达组织中始终维持在高表达水平,在接种前,表达量是对照组织的39.2倍,接种后,表达量受疫霉菌侵染诱导进一步上调,并在36 h达到最高。GmNDR1在p4XD-L5::NDR1转基因本氏烟草根中的表达量显著高于茎和叶片,表现出明显的根部表达偏好性。超表达株系接种辣椒疫霉菌15 d后的株高、根长和鲜重显著高于对照,同时叶片萎蔫率和病斑长度显著低于对照植株。【结论】 鉴定获得一个大豆根特异性表达启动子及其核心序列,融合诱导性和组织特异性启动子核心元件的人工启动子p4XD-L5驱动抗性基因GmNDR1超表达,可显著增强转基因大豆发状根和本氏烟草对疫霉菌的抗病性。  相似文献