Sexual and somatic hybridization in the genus Lactuca     

Brigitte Maisonneuve  Marie Christine Chupeau  Yannick Bellec  Yves Chupeau 《Euphytica》1955,85(1-3):281-285

Summary Various genes for disease resistance identified in wild Lactuca are difficult, even impossible to exploit in lettuce breeding, due to sexual incompatibility between L. sativa and wild Lactuca sp. We adapted two cellular biology techniques to overcome these interspecific barriers: in vitro embryo rescue and protoplast fusion. In vitro rescue of immature embryos was used successfully for sexual hybridization between L. sativa and L. virosa. Vigorous hybrid plants were produced between L. sativa and seven accessions of L. virosa. Protoplast fusion permitted the regeneration of somatic hybrids between L. sativa and either L. tatarica or L. perennis. Hybrids between L. sativa and L. tatarica were backcrossed to L. sativa.  相似文献   

体细胞杂交在油菜细胞质雄性不育创建和改良中的应用   总被引：11，自引：1，他引：11  

胡琼  李云昌 《作物学报》2006,32(1):138-143

油菜细胞质雄性不育作为生产油菜杂交种的主要授粉控制系统得到广泛利用，但由于油菜种内自然发生的有利用价值的雄性不育细胞质类型不多，所以种属间转移现有不育细胞质及发掘新型不育细胞质意义重大。体细胞杂交不仅避开了有性杂交亲和障碍的限制，还可实现两个杂交亲本细胞质基因组的重组，是一种快速有效、应用广泛的转移和诱导雄性不育细胞质的手段。本文综述了利用原生质体杂交技术在油菜种内、种间和属间转移雄性不育细胞质以及通过细胞质基因组重组改良和创建新型雄性不育细胞质的研究进展，并讨论了利用体细胞杂交方法创建新型雄性不育细胞质的应用前景。  相似文献   

The Extended Kalman Filter Data Fusion Algorithm Based on Discrete Stationary Wavelet Transformation     

LI Hong - lian  HUANG Ding - f  XIONG Yong - liang 《保鲜与加工》2006,(3):43

It is difficult to establish precise mathematical model in extended Kalman filter data fusion algorithm for GPS/ DR integrated vehicle navigation system, an improved extended Kalman filter data fusion algorithm is put forward based on Discrete stationary wavelet transformation. The simulation results show that the algorithm is better than the extended Kalman filter data fusion algorithm, and is available for GPS/DR integrated vehicle navigation system.  相似文献   

Cell lines and plants obtained after protoplast fusions of Rosa+Rosa,Rosa+Prunus and Rosa+Rubus     

Squirrell  J.  Mandegaran  Z.  Yokoya  K.  Roberts  A. V.  Mottley  J. 《Euphytica》2005,146(3):223-231

Summary Protoplasts of three Rosa cultivars were fused with each other, with protoplasts of Prunus `Colt' and with protoplasts of Rubus laciniatus, using polyethylene glycol 4000 as a fusogen. Protoplasts of Prunus were incapable of cell division and those of Rosa and Rubus were disabled by treatments with metabolic inhibitors, either iodoacetate (IOA) or rhodamine 6-G (R6G). Parental protoplasts were then fused in combinations that required complementation for their survival. RAPD analysis of 41 fusion-derived cell lines showed that two lines resulting from fusions of Rosa + Rosa and one from a fusion of Rosa + Prunus, contained some DNA markers from both fusion partners. The others contained markers of only one fusion parent. This showed that after protoplast fusion, the heterokaryons did not develop into cell lines with stable hybrid nuclei. Plants regenerated from cell lines derived from Rosa + Prunus and Rosa + Rubus fusions contained DNA markers of only Rosa and their DNA amounts were no greater than that of the Rosa parent. However, they differed morphologically from the Rosa parent to a remarkable degree, possibly because they inherited undetected genes of Prunus or Rubus, or because they were somaclonal variants of the Rosa parent. Alternative strategies for the production of somatic hybrids are discussed.  相似文献   

Further manipulation by centric misdivision of the 1RS.1BL translocation in wheat   总被引：4，自引：0，他引：4  

Adam J. Lukaszewski 《Euphytica》1997,94(3):257-261

Complete chromosomes 1R and 1B were reconstructed in wheat from the centric wheat-rye translocation 1RS.1BL. Three substitutions: 1R(1A), 1R(1B), 1R(1D), and three new centric translocations: 1RS.1AL, 1RS.1BL, 1RS.1DL were produced from the reconstructed chromosome 1R. Each one of these has the same rye chromosome arm 1RS which was present in the original translocation 1RS.1BL of ‘Kavkaz’ wheat. Reconstructed chromosome 1B and a normal chromosome 1R were used to produce a new 1RS.1BL translocation. This translocation has the long arm from the original 1RS.1BL translocation of ‘Kavkaz’, but a different 1RS arm. The third generation centric translocations were mitotically stable and were normally transmitted to progeny. Misdivision frequency of the reconstructed chromosomes 1R did not change relative to normal 1R, whereas the misdivision frequency of the two reconstructed chromosomes 1B tested was significantly higher relative to normal 1B. These experiments demonstrate that repeated cycles of centric breakage and fusion do not impair the function of centromeres in wheat and rye but may change chromosome's susceptibility to misdivision. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Fusion Expression and Purification of Marek’s disease virus Tegument Protein VP16 and Its Antibody Preparation     

邱亚峰  葛菲菲  冯秀丽  陈溥言 《农业生物技术学报》2006,14(4):530-534

马立克氏病病毒(Marek’s disease virus ,MDV)UL48基因编码的蛋白与单纯疱疹病毒1型(Herpes simplex virus ,HSV)衣壳蛋白VP16为同源物，将其克隆入pET-32a载体中，获得pET-VP16, 转化Escherichia coli BL21感受态细胞，经IPTG诱导表达，SDS-PAGE电泳显示:融合蛋白获得可溶性表达，表达蛋白的相对分子量约为67 kD；将表达的融合蛋白过His·Bind柱，获得纯化的蛋白，将该蛋白免疫家兔，制备多克隆抗体。通过间接ELISA和Western blot鉴定制备的多克隆抗体的效价和特异性，结果表明，该抗体具有较高的特异性，间接ELISA效价大于2×10－5。  相似文献   

多传感器信息融合技术及在农业工程中的应用前景   总被引：7，自引：8，他引：7       下载免费PDF全文

李小昱  王为  雷廷武  沈逸 《农业工程学报》2003,19(3):10-13

多传感器信息融合技术是20世纪80年代形成和发展起来的一种自动化信息综合处理技术，具有改善系统性能的巨大潜力。该文介绍了近年来多传感器信息技术在智能检测、智能制造、工业控制、工业机器人、智能交通、遥感以及农业工程等方面的应用研究与进展。特别指出了在农业工程领域中的应用前景广阔，目前可逐步应用于智能检测与控制系统、生产过程监视、精确农业技术、农业资源和农业环境的观测及监控方面，其将为农业生产的信息化和知识化提供技术上的支持。  相似文献   

豇豆胰蛋白酶抑制剂和硫氧还蛋白的融合表达和活性测定   总被引：2，自引：0，他引：2  

畅文军  王宇光  雷禄旺 《农业生物技术学报》2006,14(2):226-230

采用融合蛋白表达技术，通过1个人工设计合成的柔性接头，将豇豆胰蛋白酶抑制剂(cowpea trypsin inhibitor,CpTI)基因与表达载体上的硫氧还蛋白(thioredoxin )基因连接，构建了融合表达载体。将表达载体转入大肠杆菌(Escherichia coli )GI724，通过色氨酸诱导获得高效表达，产物以可溶状态存在。活性测定显示，融合蛋白具有抑制胰蛋白酶的生物学活性。以大豆胰蛋白酶抑制剂为参照，将融合蛋白热处理后进行活性测定，发现它具有较好的热稳定性。将肠激酶(enterokinase)与融合蛋白在37 ℃作用16 h，可获得切掉thioredoxin的CpTI蛋白。活性测定显示，切除thioredoxin后CpTI的胰蛋白酶抑制活性比同样处理条件下的thioredoxin-CpTI明显下降，比活力仅为原来的80%，说明融合蛋白具有更高的稳定性。研究结果为thioredoxin-CpTI作为一种生物农药的应用奠定了基础。  相似文献   

黑河流域ASTER与MODIS融合生成高分辨率地表温度的验证   总被引：2，自引：1，他引：2  

杨贵军  孙晨红  历华 《农业工程学报》2015,31(6):193-200

融合多源遥感数据生成高时空分辨率数据具有重要的应用价值。为了解决高空间分辨率数据重访周期长及云雨天气带来的数据短缺问题,该文基于增强自适应的遥感图像时空融合方法(enhanced spatial and temporal adaptive reflectance fusion model,ESTARFM),使用多时相MODIS数据提供地物时间变化信息,结合ASTER影像提供的空间细节信息,选择多波段数据(可见光近红外数据和地表温度数据)共同作为输入变量融合生成高时空地表温度。融合结果分别与地表红外辐射计观测温度和ASTER温度产品进行了验证。验证结果表明:基于ESTARFM方法降尺度地表温度影像清晰,融合结果与地表红外辐射计观测温度呈显著的线性正相关关系,相关系数均高于为0.71,预测得到的地表温度与真实测得的数据的平均绝对偏差均低于2.00 K,均方根误差均低于2.60 K。与ASTER地表温度产品的验证中,整体验证结果的R2均在0.95以上。此外,ESTARFM方法在各个地类中的融合效果较好,均表现出非植被区域的相关性高于植被和水体,尤其在2012年8月27日非植被的R2达到0.91。  相似文献   

基因融合技术及其应用   总被引：3，自引：0，他引：3  

刘岩  于涟 《农业生物技术学报》2006,14(2):273-278

基因融合技术以其快捷、高效生产多功能蛋白的优势，近年来在生物学领域中得到了愈来愈广泛的应用，结合本实验室的相关研究，简要综述基因融合技术、影响基因融合的主要因素以及融合基因的主要应用。  相似文献