Genome‐Wide Identification of MicroRNAs Responsive to High Temperature in Rice (Oryza sativa) by High‐Throughput Deep Sequencing          下载免费PDF全文

J. Li  L.‐Q. Wu  W.‐Y. Zheng  R.‐F. Wang  L.‐X. Yang 《Journal of Agronomy and Crop Science》2015,201(5):379-388

MicroRNAs (miRNAs) are known to play important roles in plant growth and stress response. Heat stress is a severe abiotic stresses by adversely affecting plant growth and yield. To identify heat‐responsive miRNAs at the genome‐wide level in rice (Oryza sativa), we constructed two small RNA libraries from young panicles treated or not with heat conditions. Ion torrent sequencing of the two libraries identified 294 known miRNAs and 539 novel miRNAs. Differential expression analysis showed that 26 miRNAs were downregulated and 21 miRNAs were upregulated in response to heat stress. Among them, five heat‐responsive miRNAs, including miR162b, miR529a‐p5, PC‐5P‐62245‐9, miR171b and miR169n, were validated by quantitative real‐time polymerase chain reaction. A total of 44 target genes of the differentially expressed miRNAs were predicted. These target genes are most significantly overrepresented in the cell growth process. The results demonstrated that rice miRNAs play critical roles in the heat stress response. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response in rice.  相似文献   

锅炉烟气余热用于木材干燥的节能改造     

何志兴  罗武生  谢洁飞  尹点 《林产工业》2016,(10):34-36

针对广东某胶合板企业2台蒸汽锅炉排烟温度高,不能根据工序用热需求实现能源梯级利用的问题,提出了锅炉烟气余热利用方案。根据干燥窑干燥木材的特点,将蒸汽锅炉的烟气引入干燥窑内的换热器用于干燥木材,每年通过回收利用的锅炉烟气余热折合标煤达439t。  相似文献   

利用蒸发冷却空调开展控温储粮试验     

曹景华  杨海涛  赖新华  郭谊  汤广宁 《粮食储藏》2016,(4):28-30

在拱板式高大平房仓的拱板间安装蒸发冷却空调开展控温储粮技术,解决拱板间的积热,以达到延缓仓内温度和上层粮温上升速度的目的。蒸发冷却空调主要利用水泵抽取水槽内的水,通过分水器均匀分布到特殊纤维制成的湿帘组件上,而大流量轴流风机的转动令仓外空气以极高速度穿过湿帘组件将湿气散发到空间,以增加空气中的湿度,通过正压促使空气流动,从而达到换气降温的效果。试验结果表明,利用低功率蒸发冷却空调,可以有效排除拱板间的积热,实现换气降温、节能、环保、绿色储粮的目的。  相似文献   

粮食烘干塔换热器智能折边设备研发     

刘军华  刘宇齐  贾富国  潘铭  孙健伦  谢帅涛 《农机化研究》2017,(8)

结合螺旋板式换热器的应用领域及其板材折边生产现状,分析了其生产弊端。针对板材折边工艺要求,研发了粮食烘干塔换热器智能折边设备,并剖析其工作机理。根据典型产品结构参数,设计智能折边装备总体装配图并计算主要零件参数,为智能折边装备制作提供理论依据,从而实现换热器板材折边智能加工,提高换热器卷板折边质量及精度。  相似文献   

电站余热淡化海水研究     

鲁海军  温高 《绿色科技》2011,(12)

提出了一种直接利用燃煤电站余热淡化海水装置，从理论上研究了最高海水温度对系统性能的影响，分析了最高盐水温度对装置的造水比和冷却海水流量的影响以及该系统的经济性。结果表明：该系统能耗小，淡化成本低，同时利用了大量余热，证明该装置具有较大的应用前景。  相似文献   

Changes in the properties of rice varieties with different amylose content on dry heat parboiling     

《Journal of Cereal Science》2015

Dry heat parboiling is a unique paddy processing technique that has been scarcely exploited. Dry heat parboiling at high temperature for short time and low temperature for long time on physical and physicochemical properties of three rice varieties differing in amylose content were studied. Hardness of the kernels increased from 66.4 N, 68.8 N and 59.8 N in raw samples to 89.1 N, 86.9 N and 59.8 N in parboiled high amylose, low amylose and waxy rice samples respectively. Rapid migration and evaporation of water from severely heated kernels caused cavity formation at the centre. Irreversible damage of amylopectin structures to leachable fractions caused continuous rise of the pasting curve. Crystallinity was thereby reduced. Parboiled high amylose samples gave X-ray diffraction patterns with peaks characteristic of A, B and V-type starch crystallinity. Crystalline starch-lipid complexes were observed in low amylose and waxy rices. The significant increase in the amount of rapidly digestible starch from 56.7%, 61.7% and 66.6% in raw samples to 92.1%, 90.8% and 94.8% respectively in severely processed rice samples and subsequent reduction in resistant starch from 24.5%, 21.2% and 18.4% to 0.4%, 1.9% and 0.1% indicated possibilities for targeted food use of the dry heat parboiled samples.  相似文献   

Action Mechanism by Which SA Alleviates High Temperature-induced Inhibition to Spikelet Differentiation     

Guan-fu FU  Cai-xia ZHANG  Xue-qin YANG  Yong-jie YANG  Ting-ting CHEN  Xia ZHAO  Wei-meng FU  Bao-hua FENG  Xiu-fu ZHANG  Long-xing TAO  Qian-y JIN 《中国水稻科学》2015,29(6):637

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茶树小分子量热激蛋白基因CsHSP17.2的克隆与表达分析     

王明乐  朱旭君  王伟东  王炫清  林明露  黎星辉 《南京农业大学学报》2015,38(3):389-394

[目的]进一步了解茶树小分子量热激蛋白基因CsHSP17.2在逆境胁迫条件下的分子生物学功能.[方法]利用RT-PCR技术从茶树‘迎霜’中克隆得到CsHSP1 7.2基因,运用生物信息学软件分析其核苷酸和编码蛋白,通过Real-timePCR分析其表达模式.[结果]该基因开放阅读框长度为453 bp,编码150个氨基酸,蛋白质相对分子质量为17.2×10a,理论等电点5.56;无信号肽位点,属于非分泌型蛋白;被定位于细胞质中.系统发育树分析表明,茶树CsHSP1 7.2与水稻(GenBank登录号:P27777)和花生(ABC41131)的进化关系较近,属于小分子量热激蛋白基因家族第Ⅰ亚族.qRT-PCR分析发现,茶树CsHSP17.2属于组成型基因;高温(38℃)处理1h能显著提高CsHSP17.2 mRNA的相对表达量(P＜0.05);干旱(100 g·L-1 PEG 6000)、高盐(200 mmol· L-1 NaCl)和外源脱落酸(200 mg· L-1 ABA)处理条件下,该基因的转录水平均出现不同程度的上调.[结论]克隆得到茶树‘迎霜’小分子量热激蛋白基因CsHSP17.2,其在花中表达量最高,且响应高温、干旱、高盐和外源脱落酸胁迫.  相似文献   

Impact of urban park’s tree,grass and waterbody on microclimate in hot summer days: A case study of Olympic Park in Beijing,China     

《Urban Forestry & Urban Greening》2018

Urban heat island (UHI) often cause negative impact and has been linked to heart stroke rate, morality, human comfort, energy consumption and air pollution. Fortunately, urban green spaces have been considered as an effective element to mitigate UHI through their cooling effect. However, further empirical research is necessary in order to efficiently guide the design and planning of urban green space. We observed the impacts of urban park's tree, grass and waterbody on microclimate inside the Olympic park of Beijing during summer days. The results indicated that, on average, the park was 0.48–1.12 °C cooler during the day, as well as increased air humidity 2.39–3.74% and reduced human comfort index 1.02–2.43 to generate more comfortable thermal environment. Urban park's cluster trees with short ground vegetation generated higher cooling effect than single trees, grass and waterbodies; proper irrigation regime enhanced the cooling effect of grasses, even the irrigated grass have similar cooling effect with small waterbody on sunny, windless summer days. Therefore, we advise to increase undergrowth coverage and grass irrigation management in order to take advantage of cooling effect of urban parks.  相似文献   

The role of green roofs in mitigating Urban Heat Island effects in the metropolitan area of Adelaide,South Australia     

《Urban Forestry & Urban Greening》2016

Changing an urban environment and replacing vegetated surfaces with low albedo materials is one of the reasons for increasing temperatures in an urban environment and consequently also one of the key causes of urban heat island effects. In this study, an experimental investigation at the micro-scale and also a numerical simulation at the macro-scale of a typical urban environment in Adelaide were conducted to estimate the potential for mitigating the UHI effect. The results showed that existing low albedo materials such as asphalt, metal roofs and brick pavements contribute to the heat island potential. Also, urban development and a lack of natural vegetation contribute to increased temperatures in cities. The ability of two types of extensive and intensive green roofs to reduce the surrounding micro-climate temperature were monitored. The results showed that they have significant cooling effects in summer time and could behave as an insulation layer to keep buildings warmer in the winter. Furthermore, different scenarios of adding green roofs to the Adelaide urban environment were investigated using the Envi–MET model. The scenario modelling of adding green roofs in a typical urban area in Adelaide, Australia, supported the hypothesis that this can lead to reductions in energy consumption in the Adelaide urban environment. Also an increased use of other water sensitive urban design technologies such as green walls and street trees together with the adoption of high albedo materials is recommended for achieving the optimum efficiency in terms of reducing urban temperatures and mitigating urban heat island effects.  相似文献