不同品系西方蜜蜂基因组DNA多态性图谱构建及杂交分析   总被引：2，自引：1，他引：1  

黄超群  蒋滢  沈伟华  蓝明扬  葛凤晨 《中国蜂业》2002,53(2):6-8

本研究应用随机引物K（5'-CGGCCCCTGT-3'）对不同品系西蜂的基因组DNA扩增，获得了能区分不同品系西蜂的多态性DNA图谱，从多态性图谱中选取一个共同片段K1680bp，标记成探针，探针K1680bp与不同品系西蜂的基因组DNA匀有杂交，从而证明片段K1680bp是西蜂蜂种的共性，为西蜂的鉴定提供了直接依据。  相似文献   

荧光原位杂交检测野生六出花(Alstroemeria aurea) 染色体命运*   总被引：4，自引：2，他引：2  

周树军 《园艺学报》2002,29(3):255-257

 利用从野生六出花(Alstroemeria aurea) 中分离的一个种特异性串联重复序列(A001-I) 作荧光原位杂交的探针, 检测野生六出花染色体在其一系列杂交后代中的命运。试验结果表明, 除最长的一对染色体外, 该方法能够检测到在其一系列杂种后代中其余7 对染色体是否存在。  相似文献   

兔防御素cDNA表达质粒的构建及其表达     

张艳宇  刘万臣  张玉静  谭芳  赵建军  柳凤琴 《中国兽医学报》2001,21(1):28-30

将兔防御素（MCP－1）cDNA插入真核表达载体pcDNA3的EcorRⅠ和XbaⅠ酶切位点之间，构建了兔MCP－1cDNA的真核表达质粒pcDEF。通过脂质体转染，使兔MCP－1 cDNA在COS－7细胞中表达，在转染60、84、108h后，提取总RNA。采用RT－PCR，在288bp的位置扩增出1条特异性带；RNA斑点印迹杂交表明，兔MCP－1 cDNA在60、84、108h均有表达。  相似文献   

寿光鸡杂交试验     

王宝维  朱新产  葛文华  张庭荣  赵德峰  韩月花  夏光顺 《中国家禽》2001,23(18):11-13

用寿光鸡与黑乌骨鸡、斗鸡和AA鸡进行杂交试验，结果表明：①寿光鸡与黑乌骨鸡杂交，其F2代体增重介于两品种之间，跖长比寿光鸡降低17．2％；羽型多表现为扁羽，约15％为丝状羽；皮肤为白色；均为5趾。②鲁西斗鸡与寿光鸡杂交F3代与寿光鸡相比，体增重降低6．3％，料重比增加5．9％，胸宽增加5．3％。③寿光鸡与AA鸡杂交，F1代增重速度比寿光鸡本身提高85．2％，料重比降低14．3％。④白乌骨鸡比黑乌骨鸡增长快，F1代比黑乌骨鸡100日龄增重提高24％，比白乌骨鸡本身增重快9．1％。  相似文献   

Meiotic and Isozymic Analyses of Tall Fescue × Giant Fescue Hybrids and Amphiploids1     

G. C. Eizenga  D. M. Burner  R. C. Buckner 《Plant Breeding》1990,104(3):202-211

The meiotic behavior of three tall fescue (Festuca arundinacea, 2n = 6x = 42) genotypes, giant fescue (F. gigantea, 2n = 6x = 42), and their reciprocal F₁ hybrids and C₁, amphiploids was evaluated to determine the parental genomic relationships. Isozyme banding patterns were used to confirm the parental identity of the hybrids and amphiploids. At meta-phase I, the parents had predominantly bivalent pairing. The hybrids had an average of 9.51 I, 16.02 II, 0.12 III, 0.02 IV, and the amphiploids had 2.17 I, 38.82 II, 0.60 III, 0.58 IV, 0.01 V—VIII. The prevalence of bivalent pairing in both hybrids and amphiploids suggested a homoeologous relationship between the six genomes, with four of the six being more closely related. Bivalent pairing in the amphiploids indicated genetic regulation of chromosome pairing. Zymograms were obtained for acid phosphatase (ACPH), alcohol dehydrogenase (ADH), glutamate oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6-PGD) and phosphoglucoisomerase (PGI). The three tall fescue and giant fescue parents had different zymograms for ACPH, MDH, 6-PGD and PGI; thus, the tall fescue parents of the hybrids and amphiploids could be determined based on the banding patterns of these four enzymes. Phenotypes were determined for ACPH-1, PGI-2 and 6-PGD-1. ACPH-1 may be used to follow the introgression of giant fescue chromatin into a certain tall fescue genotype.  相似文献   

陶赛特羊与青海半细毛羊杂交试验     

余忠祥 《青海畜牧兽医杂志》2003,33(2):8-9

试验在高寒牧区天然草地放牧条件下 ,测定陶×半细杂交一代羊出生到 4月龄和 6月龄的生长发育指标 ,结果表明 :在青海高原严酷的生态环境条件下 ,杂交一代羔羊生长发育快于青海半细毛羊 ,4月龄活重为 2 2 .73kg ,6月龄活重为 2 7.96kg ,分别比青海半细毛羔羊提高 3 .0 2kg、3 .64kg(P <0 .0 1)。体长、体高、胸围、胸宽、管围和尻宽显著高于青海半细毛羔羊 (P <0 .0 1)  相似文献   

Identification of marine algicidal Flavobacterium sp. 5 N-3 using multiple probes and whole-cell hybridization     

MASAO ADACHI  KIMIO FUKAMI  RYUJI KONDO  TOSHITAKA NISHIJIMA 《Fisheries Science》2002,68(4):713-720

ABSTRACT: We designed five 16S rRNA-targeted oligonucleotide probes (Sp probes) specific for Flavobacterium sp. 5 N-3, which inhibits the growth of red tide phytoplankton Gymnodinium mikimotoi (Dinophyceae). These probes were evaluated by whole-cell hybridization against 5 N-3 cells incubated under laboratory conditions. The fluorescence signal from the cell detected with Sp probe mix5, a mixture of the five probes, was 8.4-fold higher than that obtained with only one Sp probe (Sp01RF). The signal obtained by this method was strong enough to recognize 5 N-3 cells and count them under the epifluorescence microscope, while the signal was often undetectable when a single probe was used. Fluorescence intensities of cells at stationary phases and of 'starved' cells in sterile seawater using Sp probe mix5 were low but still sufficient for enumeration. These Sp probes did not hybridize with 11 strains from the Cytophaga/Flavobacteria/Bacteroides phylum and did react with strain 5 N-3 following whole-cell hybridization. These results show that 5 N-3 cells cultivated under our laboratory conditions can be detected by whole-cell hybridization with the five designed probes. These data also suggest that this technique may be useful for detection of an algicidal bacterium 5 N-3 in the natural environment.  相似文献   

栽培茄与水茄远缘杂交F_1植物学性状及不育性分析     

王益奎  王红  王艳娜  李文嘉  蒋雅琴  黎炎 《中国蔬菜》2015,1(1):32-36

以栽培茄116、117与野生水茄的杂交后代F1为材料,对其进行形态学分析和不育性研究。结果表明:F1多数形态特征偏向野生亲本水茄,并且表现为不育。通过对F1花粉形态、数量以及活性分析得出,其败育主要表现在花粉畸形且活力为零,花瓣不能正常开放。同时试验通过对水茄和栽培茄与杂交后代F1进行回交,然后对授粉后1~6d柱头进行荧光染色分析,发现父母本花粉与杂交后代F1柱头不亲和,不能完成正常的授粉受精。  相似文献   

Production of hybrids between Cajanus acutifolius and C. cajan     

Nalini Mallikarjuna  K.B. Saxena 《Euphytica》2002,124(1):107-110

There are many wild species of pigeonpea which are endemic to Australia. These wild species are cross incompatible with cultivated species of Indian origin. Cajanus acutifolius is one such species which does not easily cross with cultivated pigeonpea. Interspecific pollinations lead to hybrid seeds which were semi-shrivelled. Very few seeds germinated to give rise to F1 plants. Backcrossing the hybrid plants to C. cajan, the male parent, gave rise to aborting seeds which did not germinate in vivo hence BC1 plants are obtained after saving the aborting embryos in vitro. BC₁ plants showed normal meiotic pairing, but had low pollen fertility. The reasons for embryo abortion and low pollen fertility in spite of normal meiosis could be due to the effect of wild species cytoplasm. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

大豆种间杂交产量性状的遗传变异   总被引：1，自引：0，他引：1  

李莹  卫保国  任建华 《华北农学报》1986,(3)

1980年,我们用野生、半野生大豆与不同类型的栽培大豆杂交,共计10个组合.研究了杂种后代农艺性状的变异、遗传及其相互关系.结果表明:F_1;除百粒重外其它性状均有明显的杂种优势.F_2、F_3、F_4分离相当复杂,并出现许多超高亲单株和品系,其中最主要的产量性状为株高、主茎节数、每株荚数和粒数.这些性状变异幅度大,遗传力高,并与产量存在显著的正相关.有些组合百粒重与产量相关也比较明显.据我们研究,可以通过双亲百粒重预测其后代百粒重的大小,其公式为:(P_1P_2)~1/2(几何平均数)≤F_1(百粒重)<(1/2)(P_1+P_2)算术平均数.F_1与F_2,F_2与F_3百粒重之间关系密切,相关系数分别为O.9450、0.763l.这样,在F_l就可以选择百粒重.在高产×高产的组合中,后代分离高产单株和品系的机率大,实际产量也高,因此,可以早代淘汰组合.随着世代的增加,一些主要的农艺性状有明显的进化趋势,茎变粗,半直立、直立类型增多,F_2种皮分离出所有栽培大豆颜色(黄、黑、青、褐、双)及一系列的过渡色,还发现新的狸色,反映出大豆种皮色的进化历程.  相似文献