二氢杨梅素对肉仔鸡生长性能、免疫器官指数及血液生化指标的影响   总被引：2，自引：0，他引：2  

韩爱云  黄仁录  张国强  谢鹏  冯京海  张敏红 《中国饲料》2006,(10):20-22

1日龄AA肉公雏300只,随机分为5个组,每组6个重复,每个重复10只鸡。试验Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组在基础日粮中分别添加0%、0.025%、0.05%、0.1%和0.25%的二氢杨梅素(DMY),研究DMY对肉仔鸡生长性能、免疫器官指数及血液常规理化指标等影响。结果表明:1)日粮中添加不同剂量的DMY对肉仔鸡日增重有提高的趋势,0.05%的DMY显著提高肉仔鸡饲料转化率(P<0.01);试验期间,各组试验鸡生长发育正常,各组织器官未见异常。2)0.05%DMY显著提高21d肉仔鸡法氏囊指数(P<0.05);3)0.25%DMY组显著降低21d肉仔鸡血清总蛋白、白蛋白含量,对球蛋白无显著影响;4)0.025%、0.05%DMY显著降低21d肉鸡血清谷丙转氨酶(GPT)活性(P<0.05,P<0.01),0.025%～0.1%剂量的DMY能显著降低42d肉鸡血清GOT活性(P<0.05);5)各剂量的DMY对21、42d肉鸡血清谷草转氨酶(GOT)无显著影响。  相似文献   

牛、羊卵母细胞孤雌激活的研究     

彭礼繁  罗光彬 《中国奶牛》2007,(12):6-8

本试验主要比较了离子霉素、电脉冲两种方法激活牛、羊体外成熟卵母细胞的效率。两种激活方法中。牛胚胎卵裂率无显著差异（90．61％对94．40％，P〉0．05），而离子霉素激活胚胎的囊胚发育率极显著高于电激活方法（12．3％对2．4％，P〈0．01）。两种方法对羊胚胎的研究中，羊胚胎卵裂率无显著差异（72．4％对77．4％，P〉0．05）。但是离子霉素激活胚胎的囊胚发育率显著高于电激活方法（3．67％对10．40％，P〈0．05）。本试验中还比较了用化学激活法（离子霉素）激活牛体外成熟卵母细胞后，用SOFaa体系培养，换液与不换液对孤雌激活胚胎体外发育的影响。结果表明：在第4天不换液的胚胎卵裂率和囊胚率极显著高于换液的胚胎（11．64％对3．49％。P〈0．01）。  相似文献   

中草药和芦荟多糖对肉仔鸡肠道微生态、免疫功能及生产性能的影响   总被引：2，自引：1，他引：2  

戴必胜  蒋林  陈少雄 《中国家禽》2007,29(16):21-24

研究了中草药添加剂、芦荟多糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响.结果表明,肉仔鸡饲粮中添加中草药、芦荟多糖,能显著降低盲肠内容物大肠杆菌浓度,提高盲肠内双歧杆菌和乳酸菌杆菌浓度(P＜0.05);回肠微绒毛高度增加、密度加大(P＜0.05);免疫器官胸腺、脾脏、法氏囊相对质量均显著增加,新城疫抗体效价显著提高(P＜0.05);添加中草药、中草药 芦荟多糖显著提高了肉仔鸡的日增重和降低了料肉比(P＜0.05).  相似文献   

Study on Synergistic Effect of VA5 Immunopotentiator on Pigeon NDV Inactivated Vaccine     

XIONG Xiaoyan  QIN Zhenbin  MO Hongfang  HE Dongxian  YU Lintian  XU Jiarong 《中国畜牧兽医》2007,47(12):4032-4040

This study was designed to evaluate the synergistic effect of VA5 immunopotentiator on pigeon ND4416 inactivated vaccine.160 healthy pigeons were randomly divided into four groups,including ND4416 strain inactivated vaccine group (NDV group),ND4416 strain inactivated vaccine and immunopotentiator (VA5) mixed group (NDV+VA5 group),La Sota inactivated vaccine group (La Sota group) and normal saline as the control group (C group),to assess their vaccine efficacy against virulent pigeon NDV by serological analysis and animal testing.Pigeons sera were collected at different time points after immunization and measured the HI antibody titer of each group.The results showed that VA5 immunopotentiator significantly improved the serum antibody level of pigeons immunized with pigeon Newcastle disease inactivated vaccine (P<0.05).In addition,comparative test of spleen lymphocyte transformation was conducted at various time points after immunization.The results indicated that VA5 effectively stimulated the lymphocyte transformation of immune pigeon.Pigeons in each groups were challenged with ND4416 strain at the 30th,90th and 180th d after immunization.The results presented that the NDV+VA5 group had 100% protection rate and higher than La Sota group.The duration of immunization test showed that the antibody titer of NDV+VA5 group reached peak 11.20log₂ at the 21st d,remained 7.50log₂ at 180th d,and the protection rate remained 100% at 180th d.It indicated that VA5 immunopotentiator sustained the immune duration of pigeon NDV vaccine up to 180 d.Moreover,the in vitro detoxification test results suggested that VA5 immunopotentiator reduced the in vitro detoxification cycle of pigeons after challenge.Overall,this study suggested VA5 immunopotentiator could significantly improve the immune efficacy of pigeon Newcastle disease inactivated vaccine,which provided a basis for further enhancing the efficacy of Newcastle disease vaccine,as well increased experimental data for the application of immunopotentiators.  相似文献   

Monitoring the Immune Status of Broilers Against Reoviruses Using Challenge and Serologic Data     

《The Journal of Applied Poultry Research》2007,16(2):187-191

Reoviruses are an important cause of suboptimum performance in commercial broilers worldwide. Integrators use the enzyme-linked immunosorbent assay against the S1133 antigen for monitoring serum of breeders for indicating pullet vaccine success. However, without correlating serology to reovirus challenge, it is difficult to determine whether titers reflect protective immunity. We developed a broiler challenge test against 2 common reovirus isolates (2408 and S1133) to evaluate the efficacy of reovirus pullet vaccine programs. Two reovirus serologic and challenge studies were undertaken using chicks from broiler integrators from the southeastern United States. Breeder flocks, from which the chicks were obtained, received at least 1 live and 2 inactivated reovirus vaccines during their pullet phase. One-day-old progeny were collected from 6 breeder flocks. At 1 d of age, 20 chicks from each broiler flock were bled, and serum was analyzed for antibodies. At 3 to 4 d of age, 20 progeny per flock were challenged with the 2408 reovirus by intratracheal route. At 10 to 14 d of age, another 20 birds per flock were challenged with the S1133 reovirus by footpad. Twenty birds per flock were used as nonchallenged controls. At 3 wk of age, all birds were killed and weighed. Percentage of protection was calculated for each flock based on the absence of gross lesions. Flocks with at least 50% protection were considered well protected. Most flocks were well protected against both viruses. The percentage of protection correlated with day-old enzyme-linked immunosorbent assay titers. Chicks from younger hens had higher titers and the best protection against challenge. Producers, whose hen flocks were monitored herein, were doing a good job of immunizing pullets against reovirus. They are now using reovirus progeny challenge studies along with breeder antibody titers to determine vaccination success of their pullets.  相似文献   

铬对肉仔鸡生长性能、免疫功能和胴体品质的影响   总被引：5，自引：2，他引：5  

张庆东  罗绪刚  王永军  吕林  刘彬 《中国畜牧杂志》2006,42(9):19-21

用21日龄Arbor Acress(AA)肉公鸡336只,研究饲粮中添加铬对肉仔鸡生长性能、免疫功能和胴体品质的影响。将试验鸡只共分为8个处理组,分别添加源于氯化铬的铬2．0、4．0、6．0、8.0、10．0、12．0和14．0mg/kg。结果表明:饲粮中添加2．0mg/kg铬显著提高了肉仔鸡胸腺重(P<0．10),降低了腹脂率(P<0．10),4．0、6．0mg/kg铬显著提高了全净膛率(P< 0．10),各试验组显著提高了T淋巴细胞转化率(P<0．10);饲粮中添加铬对其余指标则无显著影响(P>0．10)。  相似文献   

白术及白术多糖对SD大鼠生长性能和免疫功能的影响   总被引：20，自引：0，他引：20  

胡晓蕾  胡迎利  汪以真 《中国兽药杂志》2006,40(1):2-6

在SD大鼠基础日粮中添加不同浓度的白术及其多糖，研究其对SD大鼠生长性能和免疫功能的影响。结果表明，一定剂量白术及其多糖均能有效提高SD大鼠生长性能，并能增加SD大鼠外周血白细胞的含量、NBT阳性率和血清溶菌酶含量，提高血清免疫球蛋白和补体水平，促进外周血、脾脏T／B淋巴细胞转化率，增强机体免疫功能。  相似文献   

中药"增免散"对肉鸡免疫器官及生长发育的影响   总被引：2，自引：0，他引：2  

黄军  朱红旗  吕建存  林敏  田森 《中国兽药杂志》2006,40(10):23-26

1日龄艾维茵肉鸡健雏200只随机分4组，“增免散”按饲料量0．5％、1％、1．5％分别添加到Ⅰ、Ⅱ、Ⅲ组，对照组为Ⅳ组。Ⅰ、Ⅱ、Ⅲ组从第7天开始投药，各组于第7、21天用LaSota活苗免疫，然后于第7、14、28、49天测定血液中HI抗体水平，并检测“增免散”对免疫器官和生长发育的影响。结果表明，中药“增免散”按1％、1．5％添加能显著提高Lasota活苗接种雏鸡的HI抗体水平，延长其持续时间，能促进雏鸡免疫器官的生长发育，并能促进肉鸡的生长发育，提高饲料转化率。  相似文献   

Preliminary experiments on mechanical stretch-induced activation of skeletal muscle satellite cells in vivo     

Tomowa SAKATA  Ryuichi TATSUMI  Michiko YAMADA  Sei-Ichi SHIRATSUCHI  Shinpei OKAMOTO  Wataru MIZUNOYA  Akihito HATTORI  Yoshihide IKEUCHI 《Animal Science Journal》2006,77(5):518-525

We have shown in vitro that mechanical stretch triggers activation of quiescent satellite cells of skeletal muscle to enter the cell cycle through an intracellular cascade of events including nitric oxide (NO) synthesis that results in the release of hepatocyte growth factor (HGF) from its extracellular association and its subsequent presentation to signaling receptors. In order to explore the activation mechanism in vivo, stretch experiments were conducted in the living animal using our suspension model developed. This system used the weight of the hind portion of rats to stretch the inside muscles of the left hind limb suspended for a period of 0.5–2.0 h. At the end of the stretch period, the rats received an intraperitoneal injection of bromodeoxyuridine followed by immunocytochemistry for its incorporation as an index of satellite cell activation in vivo. Depending on the period of stretch, bromodeoxyuridine labeling was increased significantly over the contralateral unstretched leg or control muscle from untreated rats. A stretched muscle extract prepared from the 2 h stretched tissue by incubating it in PBS, showed the active form of HGF as revealed by immunoblotting and it could stimulate the activation of unstretched satellite cells. Also, administering NO synthase inhibitor L‐NAME prior to muscle stretch abolished the stretch activation of satellite cells. Therefore, the results from these experiments demonstrate that stretching muscle triggers NO synthesis and HGF release, which could activate satellite cells in vivo.  相似文献   

卵母细胞激活的信号机制     

刘燕杰  孙雪萍  汪劲能  丁家桐 《畜牧兽医杂志》2007,26(1):39-41

本文主要阐述精子与卵母细胞质膜相互作用的信号转变,特别是精子启动这些信号途径的机制,并讨论与精子诱导的Ca2 振荡发生反应并在卵母细胞激活中起重要作用的下游信号分子。  相似文献