中华卵索线虫人工室内培养技术     

黎彩霞  徐慧敏  王国秀 《植物保护学报》2023,50(4):1082-1088

为室内大量繁殖中华卵索线虫Ovomermis sinensis,采用个体由大到小的斜纹夜蛾Spodoptera litura、棉铃虫Helicoverpa armigera和黏虫Mythimna separata作为宿主,测定被中华卵索线虫寄生后3种宿主的体长和体重,研究宿主种类对中华卵索线虫有效率、雌雄比和体长的影响以及寄生强度对线虫雌雄比的影响。结果显示,被中华卵索线虫寄生后,斜纹夜蛾、棉铃虫和黏虫生长减缓。在相同感染比例下（除宿主与线虫比为1∶15外）,3种宿主的线虫有效率之间差异不显著。当寄生强度为5时,3种宿主体内脱出的中华卵索线虫均为雌线虫;当寄生强度从10增到40时,斜纹夜蛾、棉铃虫和黏虫所脱出的雌性比分别由100.0%、90.0%、83.9%降为0;相同寄生强度下,从斜纹夜蛾体内脱出的雌性比显著高于从棉铃虫与黏虫体内脱出的雌性比。斜纹夜蛾、棉铃虫和黏虫体内脱出雌线虫和雄线虫平均体长分别为14.8、13.8、12.8 cm和8.0、7.5和7.5 cm。当中华卵索线虫与宿主按照比例20∶1～25∶1侵染斜纹夜蛾、按照15∶1侵染棉铃虫和黏虫时,能获得线虫最适雌雄比（1∶1...  相似文献   

昆虫病原线虫生物防控草地贪夜蛾的室内及田间效果和影响因素     

刘奇志  蔡佳仪  张小涵 《植物保护学报》2023,50(1):50-58

联合国粮食及农业组织2018年向全球发布草地贪夜蛾Spodoptera frugiperda是重要的预警害虫。据报道,2019年我国已有26个省（区、市）的作物受到草地贪夜蛾为害。目前已经发现草地贪夜蛾幼虫对某些化学农药产生抗性的现象,为缓解其抗药性产生,具有主动寻找寄主害虫的昆虫病原线虫为草地贪夜蛾的生物防治提供了应用潜能。该文从昆虫病原线虫高效侵染种（品系）及其浓度、共生菌、草地贪夜蛾幼虫易感龄期、田间致死力及影响防控效果的因素等方面进行综述,以期为田间有效利用昆虫病原线虫防治草地贪夜蛾提供参考。  相似文献   

Atypical antibiotic-responsive Staphylococcus pseudintermedius-associated tumor-like meibomitis in the upper eyelid: A case report of two dogs     

Sol Kim  Yeseul Yang  Su An Kim  SeungJun Lee  Yongbaek Kim  Kangmoon Seo  Seonmi Kang 《Veterinary ophthalmology》2023,26(5):458-463

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Chronic tuberculosis caused by Mycobacterium bovis in a domestic donkey in Central Europe     

Natalia Siwinska  Agnieszka Zak  Malwina Slowikowska  Artur Niedzwiedz  Rafal Ciaputa  Izabela Janus  Marek Lipiec  Lukasz Radulski  Adam Szymerowski  Marcin Nowak 《Equine Veterinary Education》2023,35(2):e61-e65

Bovine tuberculosis is a contagious and zoonotic disease of animals and humans. In Europe, the number of reported cases of tuberculosis has decreased. Equidae are relatively rarely infected even in endemic areas. The presented report describes a case of chronic Mycobacterium bovis tuberculosis in a 30-year-old female donkey. The donkey initially presented with persistent lymphadenopathy; however, as the disease progressed, weight loss became apparent. To the authors' knowledge, this is the second confirmed case of tuberculosis in a donkey in Europe.  相似文献   

Characterisation of faecal microbiota in horses medicated with oral doxycycline hyclate     

Ronan J. J. Chapuis  Anne A. M. J. Becker  Patricia M. Dowling  J. Scott Weese 《Equine veterinary journal》2023,55(1):129-141

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影响粉碎机工作性能因素的分析   总被引：2，自引：0，他引：2  

毕晓伟  杨昕宇  阚志新  高立柱 《农机化研究》2005,(6):74-75

锤片式粉碎机是一种利用高速旋转的锤片来击碎饲料的机器。它具有通用性广,对饲料的温度敏感性小,调节粉碎方便,粉碎质量好,使用维修方便和生产率高等优点。但是,由于用户对影响饲料粉碎机的工作性能的主要因素分析掌握不够,致使生产率不高、消耗的动力较大,产品质量等指标无法保证。为此,列举了影响粉碎机工作性能的诸因素,为广大用户高效、优质、低耗、安全地使用粉碎机提供了理论基础。  相似文献   

A role for Toll-like receptor 4 in the host response to the lung infection of Yersinia pseudotuberculosis in mice     

《Comparative immunology, microbiology and infectious diseases》2016

Although a Yersinia pseudotuberculosis (Yptb) lung infection model has been developed to study Y. pestis pathogenesis, it is still necessary to establish a new animal model to mimic the pathophysiological features induced by Y. pestis infection. Here, we provide a new lung infection model using the Yptb strain, IP2777, which displayed rapid spread of bacteria to the liver, spleen, and blood. In addition, we examined whether TLR4 is involved in Yptb-induced pathogenesis in the lung infection model of mice we generated. Following lung infection of WT and TLR4-deficient mice with the Yptb strain IP2777, the survival rate, bacterial colonization, histopathology, and level of cytokines and chemokines in the lung, spleen, liver, and blood were analyzed. TLR4-deficient mice had a lower survival rate than WT mice in response to Yptb lung infection. Although the bacterial colonization and pathology of the lung were comparable between WT and TLR4-deficient mice, those of the spleen and liver were more severe in TLR4-deficient mice. In addition, the levels of TNF-α and CXCL2 in the liver and IL-6 and CXCL2 in the blood were higher in TLR4-deficient mice than in WT mice. Our results demonstrate that TLR4 is necessary for optimal host protection against Yptb lung infection and TLR4-deficient mice may serve as a better genetic model of Yptb infection for mimicking Y. pestis infection.  相似文献   

Genetic diversity and mixed infections of begomoviruses infecting tomato, pepper and cucurbit crops in Nicaragua   总被引：1，自引：0，他引：1  

M. Ala-Poikela  E. Svensson ‡  A. Rojas  T. Horko  L. Paulin  J. P. T. Valkonen  A. Kvarnheden † 《Plant pathology》2005,54(4):448-459

Begomoviruses were detected in Nicaraguan fields of tomato ( Lycopersicon esculentum ) and adjacently growing plants of pepper ( Capsicum annuum ), chilli pepper ( C . baccatum ), cushaw ( Cucurbita argyrosperma ) and Mexican fireplant ( Euphorbia heterophylla ) using polymerase chain reaction (PCR) and universal begomovirus primers. All tomato and Mexican fireplant plants showing symptoms were infected with begomoviruses, while only 30–46% of the pepper, chilli pepper and cushaw plants showing symptoms tested virus-positive. No begomoviruses were found in potato. The virus species were provisionally identified by sequencing 533 bp of the viral coat protein gene ( AV1 ). Tomato severe leaf curl virus (ToSLCV), Tomato leaf curl Sinaloa virus (ToLCSinV) and Pepper golden mosaic virus (PepGMV) were found to infect both tomato and pepper. A new provisional species designated Tomato leaf curl Las Playitas virus (ToLCLPV) was detected in a tomato plant. Squash yellow mottle virus (SYMoV) and PepGMV were found in cucurbits, the latter for the first time in this host. Euphorbia mosaic virus (EuMV) was detected in Mexican fireplant. Sequencing of a larger number of PCR-amplified clones from selected plants revealed intraspecific viral sequence variability, and also multiple begomovirus infections which could represent up to three species in a single tomato or cushaw plant. Phylogenetic grouping of virus sequences did not correlate with the host of origin.  相似文献   

大豆疫霉菌对大豆下胚轴侵染过程的细胞学研究   总被引：3，自引：0，他引：3  

左豫虎  康振生  黄丽丽  韩青梅 《植物病理学报》2005,35(3):235-241

 接种后1.5~24h,用光镜和电镜研究了2个大豆品种与大豆疫霉菌Ps411的亲和性和非亲和性互作。观察结果表明,大豆疫霉菌对大豆下胚轴的侵染过程可分为侵入前、侵入、皮层组织中的扩展和进入维管束组织4个连续阶段。大豆下胚轴接种后在25℃保湿培养,1.5h后游动孢子即形成休止孢并萌发产生附着孢,3h后侵入表皮细胞,6h后进入皮层组织,24h后进入维管束组织。病原菌主要以侵染菌丝直接侵入表皮,表皮细胞间隙是主要侵入部位。皮层细胞是病原菌定殖和发展的主要场所,胞间菌丝侵入皮层细胞并形成吸器。在菌丝与寄主细胞接触部位的寄主细胞壁与质膜之间常有胞壁沉积物的形成。在抗病品种上病菌的侵染事件与感病品种基本一致,但不能形成正常的吸器,胞壁沉积物明显多于感病品种,菌丝在寄主组织内的扩展明显受到抑制。利用β-1,3-葡聚糖免疫金标记单克隆抗体进行的免疫细胞化学的研究表明,胞壁沉积物内含有大量的β-1,3-葡聚糖,在大豆疫霉菌菌丝壁中也存在β-1,3-葡聚糖。以上结果表明,病原菌的侵染可诱导抗病寄主细胞内β-1,3-葡聚糖迅速的合成与积累、并形成胞壁沉积物,以抵御病菌的侵染与扩展。  相似文献   

The role of fungal endophyte infection in the evolution of Lolium multiflorum resistance to diclofop-methyl     

M M Vila Aiub  & C M Ghersa 《Weed Research》2001,41(3):265-274

Diclofop-methyl resistance was evaluated in populations of Italian ryegrass ( Lolium multiflorum Lam.) infected and uninfected with fungal endophyte ( Neotyphodium ). Survival was tested in susceptible populations using herbicide screening. The results served as inputs to a model for investigating the role of endophyte infection in the evolution of L. multiflorum resistance to herbicide. The tolerance of infected plants varied depending on the origin of the population and the herbicide dose. Only in some populations and at some diclofop-methyl doses did plants infected with these endophytes have higher survivorship than endophyte-free plants. The model demonstrated that endophytes might indeed play an important role in the evolution of herbicide-resistant weeds, delaying the appearance of herbicide resistance.  相似文献