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71.
赤峰市松山区土地利用动态变化及其原因分析   总被引:8,自引:6,他引:8  
以TM遥感资料为信息源 ,利用 3S技术手段 ,对内蒙古自治区赤峰市松山区 1987,1993,2 0 0 0年的土地利用变化进行了分析。结果表明 ,在不同年度内 ,其主要的土地利用类型为耕地、草地和林地。土地利用类型的总量变化也以这 3种土地利用类型和建设用地变化最大。而从年变化速率看 ,1987- 2 0 0 0年 ,以建设用地的增加和未利用土地类型的减少最为快速。从土地利用类型转化的方向看 ,以耕地、草地和林地相互之间的转化最为频繁。从土地利用类型的空间稳定性看 ,耕地的稳定性最高 ,其次为草地 ;而最不稳定的土地利用类型为未利用土地。利用相关分析所作的土地利用变化动因显示 ,该地区土地利用变化的驱动因素主要是社会与经济因素 ,其中以总人口、农业总产值、粮食作物产量总计、农牧民人均纯收入、化肥使用面积和化肥实物量等因子的作用最大 ,而自然因素对该地区土地利用变化的影响极小。  相似文献   
72.
以包头市城郊区为例 ,在地理信息系统支持下 ,以乡镇 (苏木 )为研究单元 ,研究本地区土地利用时空动态变化过程 ,并分析其变化驱动因素。从微观角度分析了北方农牧交错区城郊区土地利用变化特征。农牧交错区是人类活动影响强烈之地带 ,土地利用变化与土地持续研究利用 ,对生态安全和地区持续发展具有十分重要的意义。  相似文献   
73.
不同覆盖方式对旱地玉米田土壤环境及玉米产量的影响   总被引:20,自引:0,他引:20  
分析普通地膜覆盖、小麦秸秆覆盖及露地栽培3种不同处理对旱地玉米田土壤环 境、玉米产量构成因素的影响,结果表明:覆盖栽培能改善土壤环境,降低土壤容重,提高土壤 水分利用率,调节土温、湿度,协调水热资源利用的同步性;秸秆覆盖能增加土壤养分含量,特 别是速效钾含量;在玉米生长后期,提高叶面积指数,延长叶片功能,提高玉米的光合能力从而 防止玉米早衰,增加穗重,提高玉米产量。  相似文献   
74.
以Taichung29为背景的小麦抗条锈病近等基因系转育进展   总被引:6,自引:0,他引:6  
自1988年起,系统开展了以Taichung29为背景的小麦抗条锈病近等基因系转育及其基础性研究。采用回交法和系谱法相结合的转育方法,以春性品种Taichung29为轮回亲本作母本,分别与25个抗性供体即中国小麦条锈病菌鉴别寄主和国际上重要的抗条锈基因载体品种杂交、回交和自交。通过基因推导分析、单体分析和SSR标记技术检测目的基因,选育抗条锈近等基因系,现已获得重要进展,成功选育出8个以Taichung29为背景的抗条锈病单基因近等基因系,即Taichung29*6/Yr1、Taichung29*6/Yr2、Taichung29*6/Yr5、Taichung29*6/Yr7、Taichung29*6/Yr9、Taichung29*6/Yr10、Taichung29*6/YrSpP、Taichung29*6/YrKy2。另有9个组合转育获得343个抗性稳定株系,正检测其目的基因,3个组合转至BC6F3,自交纯合筛选抗性稳定株系,5个组合转至BC5,继续回交转育。  相似文献   
75.
AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.  相似文献   
76.
AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-β1 in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM、 TGF-β1、 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-β1 and reducing excessive accumulat ion of laminin and type IV collagen.  相似文献   
77.
The present study examines the contribution of the nucleus to meiotic competence in mouse oocytes that were reconstructed using nuclear transfer. Three types of reconstructed oocytes were produced: MP‐GV, by transplanting the male pronucleus (MP) into germinal vesicle (GV) stage oocytes; 3T3‐GV, by transplanting the nucleus of a National Institute of Health (NIH) 3T3 cell into a GV stage oocyte; and 3T3‐MII, by transplanting the nucleus of an NIH 3T3 cell into a metaphase II (MII) stage oocyte. The fusion rates differed, but not significantly, in the MP‐GV, 3T3‐GV, and 3T3‐MII groups (77, 63, 56%, respectively). Then, meiotic competence was compared in MP‐GV, 3T3‐GV and non‐manipulated GV stage oocytes as a control. Nuclear envelope breakdown occurred in all the reconstructed oocytes, as well as the control ones. The percentage of first polar body extrusion differed between the MP‐GV (100%), 3T3‐GV (72%), and control (67%) groups. DNA staining with Hoechst 33342 revealed that in the MP‐GV‐group oocytes that had reached MII stage, the chromosomes were condensed and aligned in a regular array similar to the normal metaphase plate. By contrast, in 3T3‐GV group oocytes, the condensed chromosomes were irregularly scattered in the cytoplasm. These results suggest that the donor nucleus affects meiotic competence in reconstructed oocytes.  相似文献   
78.
The current paper describes aspects of local immunity in the ovary and oviduct, and the significance of immunity to reproductive functions in hens. The immunocompetent cell populations in the ovary and oviduct change with a positive correlation to sexual activity, and gonadal steroid is one of the key factors in the increase. Local immune responses mediated by major histocompatibility complex class II and T cell subsets occur in response to infection by Salmonella enteritidis, which may contaminate eggs. In the ovary, immunocompetent cells are also suggested to play roles in the regulation of ovarian functions. Macrophages and T cells are likely to enhance the regression of atretic follicles to maintain the ovarian tissue microenvironment. Autoantibodies to ovarian tissues appeared in the hens with low egg laying frequency, suggesting that the auto‐antibodies may be one of the factors in the decline of egg production. In the oviduct, local immunity possibly has a role in the selection of sperm, though the immunoreactions may also affect sperm survival leading to the decline in fertility. The concentration of yolk IgY, which plays a role in maternal immunity transmission, significantly decreases with the aging of birds, whereas it is significantly increased by estrogen. Therefore, the immune system plays significant roles not only in defense against infection, but also in the functions of reproductive organs. Investigations on the local immune system in the reproductive organs and factors affecting it are of importance for the production of sterile eggs and improvement of reproductive functions.  相似文献   
79.
选择9头母牛先行人工授精、7天后再移植冷冻胚胎以生产双犊。试验结果表明,母牛产双犊达44.44%(P<0.01)。提示:采用A(I人工授精)+ET(胚胎移植)是提高母牛双犊率的有效技术措施之一。  相似文献   
80.
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