Pesticide—induced reproductive dysfunction in Indian fishes     

B. Lal 《Fish physiology and biochemistry》2007,33(4):455-462

Numerous studies are available reporting the effects of pesticides on reproductive activity in Indian fishes. The majority of these reports deals with histopathological changes in gonads and endocrine glands involved in the regulation of reproduction following treatment with different pesticides. Pesticides are reported to cause degenerative changes in gonads and arrest gametogenic processes either by acting directly on the gonads or by interfering with the secretory activity of the hypothalamo-hypophyseal-gonadal/thyroid axis that regulates various reproductive events. Secretion of hormones such as gonadotropin-releasing hormone (GnRH), gonadotropin, growth hormone, adrenocorticotropic hormone (ACTH), testosterone, estrogens, 17,20β-dihydroxyprogesterone and thyroid hormones are in general lowered, leading to cessation of gametogenesis, vitellogenesis, oocyte maturation, ovulation, spermiation, etc. Adverse effects of pesticides have also been demonstrated on fecundity, fertilization, hatching, and postembryonic development. The effects are highly variable and depend on the nature, dose, and mode of application of the pesticides.  相似文献   

Genetic covariation in production traits of sub-adult black bream Acanthopagrus butcheri after grow-out     

Robert G Doupé  & Alan J Lymbery 《Aquaculture Research》2005,36(11):1128-1132

Predicting the suitability and reliability of traits associated with juvenile growth as indirect selection criteria for choosing future broodstock requires accurate and repeatable estimates of genetic (co)variation for growth traits at different ages. We compared juvenile wet weight of black bream Acanthopagrus butcheri (Munro) at 6 months of age with wet weight, dressed weight, fillet yield and gonad weight in tagged individuals at 18 months of age, following 12 months of farm grow‐out. Fish survival and tag retention was high, and there was significant among‐family variation for all traits. The phenotypic correlations among wet weight, dressed weight and fillet yield at 18 months of age were very high (0.93–0.97) and similar to their genetic correlations (0.96). Importantly, the phenotypic correlations between wet weight at 6 months and wet weight, dressed weight and fillet yield at 18 months were high (0.63–0.65), and so too were their genetic correlations (0.66–0.73), indicating the potential for using wet weight in the hatchery as a selection criterion for improved weight and meat yield of fish at harvest. Gonad weight shared little or no phenotypic or genetic correlation with these other traits, suggesting that selection for faster growing fish will not affect fecundity or sexual maturation rate. It appears, however, that cultured black bream do become sexually mature more rapidly than wild fish, as 78% of all fish harvested in this study had developing or mature gonads, whereas less than 50% of fish in wild populations are reproductively mature by the same age. Precocious sexual development may lead to uncontrolled spawning in grow‐out ponds and a potential loss of selection gains.  相似文献   

Induced spawning of hatchery-raised Brazilian catfish, cachara Pseudoplatystoma fasciatum (Linnaeus, 1766)     

Antnio Fernando Gervsio Leonardo  Elizabeth Romagosa  Maria Inês Borella  Sergio Ricardo Batlouni 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):451-461

In the months of January 2001 and 2002, female cachara Pseudoplatystoma fasciatum were selected during their first and second gonadal maturation (2 years and 7 months old and 3 years and 7 months old, respectively) with an of oocyte diameter of 937.5 μm (82.5% with central nuclei and 17.5% with peripheral nuclei). Nine females in first maturation received two doses of carp pituitary extract (CPE), 0.5 mg/kg and 5.0 mg/kg; seven received two doses of human chorionic gonadotropin (hCG), 5 and 10 IU/g; five received doses of 0.5 CPE mg/kg and 5 hCG IU/g (CPE+hCG); and four received 0.9% saline (saline). Nine females from CPE and seven from hCG presented oocytes with the same diameter at the moment of oocyte release (100% with germinal vesicle breakdown and fertilization rate of 53.44±18.3 and 54.81±11.8%; larvae number of 165,330±94.1 and 158,570±20.6, respectively). The five females from CPE+hCG did not respond to the hormonal treatment. The four females from the saline group did not ovulate. In January 2002, 6 of 15 selected females that were going through the second reproductive cycle received CPE (five received hCG and four received saline), showing oocyte diameters similar to the ones in the first maturation. At stripping, CPE females had an oocyte diameter of 1062.5 μm (the hCG females had oocyte diameters ranging from 937.5 to 1125.0 μm; fertilization rates of 56.08±30.9 and 81.90±17.3%; 364,547±244 and 633,129±190, larvae, respectively). The fertilization rates and larvae number were higher in the second gonad maturation, both for CPE and hCG.  相似文献   

Reproductive parameters of the chub mackerel <Emphasis Type="Italic">Scomber japonicus</Emphasis> estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity     

Tetsuro?Shiraishi  Kohei?Ohta  Akihiko?Yamaguchi  Mari?Yoda  Hisashi?Chuda  Michiya?MatsuyamaEmail author 《Fisheries Science》2005,71(3):531-542

ABSTRACT:   Final oocyte maturation and ovulation of captive chub mackerel Scomber japonicus with fully yolk-accumulated oocytes were induced by a single injection of human chorionic gonadotropin. Reproductive parameters, including spawning frequency and batch fecundity, which are required to estimate spawning biomass in pelagic fish by the daily egg production method, were analyzed. Germinal vesicle migration (GVM) occurred at 18–24 h post-injection, and the hydration and ovulation of oocytes were completed at 30 and 36 h post-injection, respectively. The results of the maturation process suggest that fish with GVM-stage ovaries captured in the daytime from the field are capable of spawning on the night following their capture. The oocytes used in the oocyte size-frequency distribution method for batch fecundity estimates should be at late GVM and more advanced stages. The results of sequential artificial insemination showed that the quality of ovulated eggs held in the ovarian lumen rapidly deteriorated as time progressed after ovulation. This indicates that the fertilization window for the ovulated eggs of chub mackerel lasts only a few hours, and spawning behavior should be performed within a few hours after ovulation in the wild population.  相似文献   

Circulating levels and in vitro production of two maturation-inducing hormones in teleost: 17α,20β-dihydroxy-4-pregnen-3-one and 17α,20β,21-trihydroxy-4-pregnen-3-one, in a daily spawning wrasse, Pseudolabrus japonicus     

M. Matsuyama  K. Ohta  S. Morita  M.M. Hoque  H. Kagawa  A. Kambegawa 《Fish physiology and biochemistry》1998,19(1):1-11

The female bambooleaf wrasse, Pseudolabrus japonicus, spawns daily during the spawning season, and exhibits a diurnal rhythm of ovarian development. In the present study, we have investigated: (1) circulating levels of 17a, 20-dihydroxy-4-pregnen- 17,20-P) and 17,20,21-trihydroxy-4-pregnen-3-one (20-S) in females sampled at different times of the day during spawning season in captivity, and (2) in vitro production of 17,20-P and 20-S by follicle-enclosed oocytes at seven different develo tal stages. In addition, we developed a microtiter plate enzyme-linked immunosorbent assay (ELISA) for 17,20-P. Serum levels of 17,20-P and 20-S showed similar diurnal changes; substantial increases in these levels occurred around the time of germinal vesicle breakdown (GVBD). In vitro experiments showed that massive production of 17,20-P and 20-S occurred in follicles collected just before or during GVBD. Further, acute decreases in 17,20-P and 20-S production were found in the ovarian follicles just prior to ovulation, suggesting inactivation of the maturation-inducing hormone (MIH). These results, taken together with our previous data on the occurrence of GVBD in vitro, suggest a role for both 17,20-P and 20b-S as MIHs in the bambooleaf wrasse.  相似文献   

磷脂氢谷胱甘肽过氧化物酶在精子发育中的作用研究进展     

蔡丝丝  刘金  王林川  陈芳艳 《广东农业科学》2014,41(16):116-119

磷脂氢谷胱甘肽过氧化物酶(PHGPx)是广泛存在于哺乳动物细胞内唯一能够物接还原生物膜上脂类过氧化物的一类含硒酶.该酶在保护细胞膜完整、维持细胞的正常功能中发挥着重要作用;同时PHGPx还作为精子的结构物质,在精子的发育和成熟过程中发挥着重要作用;精液中PHGPx活性是评价精液品质的重要参数之一.对PHGPx的基因结构、体内分布以及与精子发育与成熟相关的生物学特性和功能等研究进展进行了总结.  相似文献   

Density effects on length at maturity of walleye pollock <Emphasis Type="Italic">Theragra chalcogramma</Emphasis> off the pacific coast of northern Japan in the 1990s     

Tomonori Hamatsu  Keizou Yabuki 《Fisheries Science》2007,73(1):87-97

To determine how stock abundance fluctuations of walleye pollock Theragra chalcogramma are related to variations in reproductive characteristics, the length at 50% maturity of the fish sampled off the Pacific coast of northern Japan from 1990 to 1999 was examined. In both sexes, the fish density increased, and the body length of age-3-5 fish decreased, but the condition factor and the age at 50% maturity showed no clear trend during this period. Male length at 50% maturity decreased, while value for females showed no clear trend. In both sexes, significant negative correlations were found between density index (combined age-3-5) and each body length at age-3-5. Significant positive correlations were seen between each body length at age-3-5 and length at 50% maturity in males. Positive correlation between body length at age-5 and length at 50% maturity was significant in females from 1990 to 1999, except for the samples in 1998. We suggest that the fish density might affect the length at maturity in males and possibly also in females.  相似文献   

Increase of otolith Sr:Ca ratio related to metamorphosis and maturation in anchovy <Emphasis Type="Italic">Engraulis japonicus</Emphasis>     

Hiromu Zenitani  Naoaki Kono  Youichi Tsukamoto  Noritaka Mochioka 《Fisheries Science》2007,73(6):1395-1397

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发情牛血清在猪卵母细胞成熟中的应用     

李秋艳  朱庆鸿  卫恒习  李燕  郭英  戴蕴平  李宁 《农业生物技术学报》2010,18(1):192-197

为了检验发情牛(Bos taurus)血清在猪卵母细胞成熟中的应用,实验分别在成熟培养液中加入不同的血清,观察卵母细胞的成熟效果,并通过孤雌激活胚胎和重构胚的体外发育能力来判断血清对卵母细胞质量的影响:研究还进行了用发情牛血清成熟的卵母细胞构建的核移植胚胎的体内发育试验.结果表明,发情牛血清,成年牛血清和胎牛血清在猪卵母细胞的成熟率方面没有显著差异(P>0.05);卵母细胞成熟时加入不同的血清,对卵母细胞体外发育能力的影响不显著(P>0.05);来自含发情牛血清成熟液培养的卵母细胞,与胎儿成纤维细胞构建的克隆胚胎能够完成全期发育.  相似文献   

Reproductive biology of the cardinalfish Apogon lineatus in Tokyo Bay, Japan     

Gen Kume  Atsuko Yamaguchi  Ichiro Aoki and  Toru Taniuchi 《Fisheries Science》2000,66(5):947-954

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