蔬菜中残留毒死蜱的检测方法     

喻龙  李光义  邓晓  李勤奋 《勤云标准版测试》2007,(12)

通过对5种蔬菜中添加毒死蜱标样的回收率试验,研究蔬菜毒死蜱监测方法中震荡提取时间、淋洗液配比、层析硅胶用量、淋洗液选择时间等关键步骤的最佳定量组合。结果表明提取时间为30min,淋洗液用单一的丙酮,层析柱中填充1g硅胶,淋洗过程中取6min以后的过柱淋洗液做色谱分析,既可大量清除蔬菜中含有的对色谱峰的干扰因素,同时也保证回收率达到90%～120%之间,得到较好的效果。  相似文献   

A General Method for QTL Mapping in Multiple Related Populations Derived from Multiple Parents     

AO Yan  HU Zhi-qiu  TANG Zai-xiang  WANG Xue-feng  XU Chen-wu 《水稻科学》2009,16(1):45-50

It’s well known that incorporating some existing populations derived from multiple parents may improve QTL mapping and QTL-based breeding programs. However, no general maximum likelihood method has been available for this strategy. Based on the QTL mapping in multiple related populations derived from two parents, a maximum likelihood estimation method was proposed, which can incorporate several populations derived from three or more parents and also can be used to handle different mating designs. Taking a c...  相似文献   

高效液相色谱法检测玉米及玉米叶中乙草胺的残留量   总被引：1，自引：0，他引：1  

刘晓明  郭平毅  冯翠萍  王振 《山西农业大学学报(自然科学版)》2012,32(1):5-8

建立了高效液相色谱法测定玉米及玉米叶中除草剂乙草胺的分析方法。玉米用乙腈提取,玉米叶用乙酸乙酯提取,经过固相萃取小柱净化处理,用HPLC检测。当乙草胺的添加量在0.01～0.5mg·kg-1水平下,乙草胺在玉米和玉米叶中添加回收率分别为85.47%～90.67%和93.03%～98.24%,相对标准偏差为1.497%～2.177%和1.113%～1.629%。此方法灵敏、可靠,满足农药残留分析的要求。  相似文献   

活体生物在农药残留快速检测中的研究应用     

朱华  宋航  边阔 《安徽农学通报》2012,18(1):161-162,170

农药残留超标是影响农业生产中蔬菜品质提高的重大因素之一。农药残留检测技术朝着简单、快速、高效、准确的的方向发展,在实际应用中,已有的检测技术有着费用高、检测耗费时间大等缺陷。该文从来源广泛、检测灵敏、检测费用低、实用性强等方面着手,认为通过筛选敏感昆虫应用在农药残留快速检测中,有着更为实际的意义。  相似文献   

柑橘中13种农药残留量的检测     

杨群华  陈鹤玲  邓子尧  徐伟松  江燕玲 《农药科学与管理》2009,30(2):21-25

本文介绍了柑橘样品中农药残留监测分析的研究，结果表明利用乙腈对柑橘中氧乐果等13种农药进行提取、经固相萃取柱净化、气相色谱仪带FPD、ECD检测器定量检测，13种农药分离的效果良好，回收率为82．3％～95．0％，相对标准偏差〈5．0％。该方法是对NY／T761—2008的优化．指出了实际分析过程中应注意的问题。优化后的方法精密度高、重现性好，可以为广东省柑橘农药残留检测工作中推广应用。  相似文献   

Gas Chromatography–Mass Spectroscopy Analysis of Carbofuran and Its Metabolite 3-Hydroxy-carbofuran in Maize and Soil in Field     

《Communications in Soil Science and Plant Analysis》2012,43(11):1316-1323

In this article, a simple and efficient method using gas chromatography–mass spectrometry (GC-MS) was developed to determine carbofuran and 3-hydroxy carbofuran in maize and soil. Field experiments were carried out to investigate the dissipation of carbofuran and its metabolite 3-hydroxy carbofuran in soil and maize plants and their accumulation in maize. Results showed that the half-lives of carbofuran were 4.7 and 5.3 days in maize plants and 8.5 and 7.6 days in soil for Beijing and Jinan trial sites, respectively. The final residues of carbofuran and 3- hydroxy carbofuran in the soils and maize grain were not detected. This study suggests that carbofuran is acceptable to apply to maize in the recommended dosage and can be a good alternative to other highly toxic insecticides.  相似文献   

大米除虫脲残留量测定     

王功 《吉林粮食高等专科学校学报》2000,15(3):4-7

采用粮谷中残留分析样品前处理的微量化学法（ＭＡＣＣＭ）技术，以快速、简便的提取净化方法，应用ＨＰＬＣ对大米中除虫脲残留量的检测，达到了满意效果，重视性和回收率均好，检测低限达０．０２ｍｇ／ｋｇ。具备通用操作价值。  相似文献   

用初孵幼虫监测棉红铃虫对杀虫剂的抗性   总被引：3，自引：0，他引：3  

李显春  王荫长  韩召军  王沫  丁士银  董习华  骆寿山  陈彩霞 《植物保护学报》1995,22(1):85-90

本文通过试验提出了一种准确、简易、快速的棉红铃虫抗药性测定方法－初孵幼虫滤纸药膜法。并利用这种方法测定了棉红铃虫田间种群对甲基对硫磷和溴氰菊酯的抗性，结果发现安徽安庆、湖北江陵和天门３个种群对溴氰菊酯分别产生了２０３．８、３．７和４．２倍抗性，安庆种群还对甲基对硫磷产生４．４倍的低水平抗性，而浙江慈溪种群对甲基对硫磷的敏感性正在降低，ＬＤ５０值增高２．４倍。这些结果与当地的实际用药情况相符，说明初  相似文献   

Dissipation of cyproconazole and quinalphos on/in grapes     

Euphemia Papadopoulou-Mourkidou  Athena Kotopoulou  George Papadopoulos  Christos Hatziphanis 《Pest management science》1995,45(2):111-116

The persistence of cyproconazole and quinalphos on/in grapes was investigated when both compounds were applied to vines at the locally recommended application frequencies and rates and at double these rates, using commercially available formulations. Residues of cyproconazole applied at recommended and double the recommended rates of application in/on grapes immediately after the last application were 0-049 (±0.034) and 0.077 (±0.008) mg kg^?1, respectively, reduced to 0.011 (±0.003) and 0.018 (±0.010) mg kg^?1 respectively seven days after the last application. The corresponding residue levels of quinalphos immediately following the last application were 1.42 (±0.10) and 3.36 (±0.07) mg kg^?1, reduced to 0.043 (±0.002) and 0.072 (±0.028) mg kg^?1 respectively 21 days after the last application. Cyproconazole, being systemic, is rapidly absorbed by the grape tissues and its residues dissipate with a half-life of three to four days, while quinalphos, being non-systemic, dissipates faster with a half-life of two or three days. The residues of both pesticides were analysed by a GLC-NPD system.  相似文献   

桃中残留有机磷农药去除方法研究   总被引：4，自引：0，他引：4  

李鹏坤  李巧枝  刘芳  马丽娜  李华玮  邵彦来  游永  王青霞  郝晓磊 《农药科学与管理》2004,25(6):16-20

本文选用洗洁精、食用碱、洗洁精加食用碱水溶液和自来水。对喷施过有机磷杀虫剂甲基对硫磷、毒死蜱的桃子进行浸渍处理。结果证明，食用碱水溶液对农药残留去除效率可达34．26％～57．23％，对毒死蜱药后7d采摘的鲜桃处理后基本可达食用标准．而甲基对硫磷仍然超标，供试洗洁精无去除作用。  相似文献