Equine platelet lysate as an alternative to fetal bovine serum in equine mesenchymal stromal cell culture – too much of a good thing?          下载免费PDF全文

K. A. Russell  T. G. Koch 《Equine veterinary journal》2016,48(2):261-264

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中华卵索线虫人工室内培养技术     

黎彩霞  徐慧敏  王国秀 《植物保护学报》2023,50(4):1082-1088

为室内大量繁殖中华卵索线虫Ovomermis sinensis,采用个体由大到小的斜纹夜蛾Spodoptera litura、棉铃虫Helicoverpa armigera和黏虫Mythimna separata作为宿主,测定被中华卵索线虫寄生后3种宿主的体长和体重,研究宿主种类对中华卵索线虫有效率、雌雄比和体长的影响以及寄生强度对线虫雌雄比的影响。结果显示,被中华卵索线虫寄生后,斜纹夜蛾、棉铃虫和黏虫生长减缓。在相同感染比例下（除宿主与线虫比为1∶15外）,3种宿主的线虫有效率之间差异不显著。当寄生强度为5时,3种宿主体内脱出的中华卵索线虫均为雌线虫;当寄生强度从10增到40时,斜纹夜蛾、棉铃虫和黏虫所脱出的雌性比分别由100.0%、90.0%、83.9%降为0;相同寄生强度下,从斜纹夜蛾体内脱出的雌性比显著高于从棉铃虫与黏虫体内脱出的雌性比。斜纹夜蛾、棉铃虫和黏虫体内脱出雌线虫和雄线虫平均体长分别为14.8、13.8、12.8 cm和8.0、7.5和7.5 cm。当中华卵索线虫与宿主按照比例20∶1～25∶1侵染斜纹夜蛾、按照15∶1侵染棉铃虫和黏虫时,能获得线虫最适雌雄比（1∶1...  相似文献   

凝结芽孢杆菌对热应激肉鸡生长性能及肠道消化与吸收功能的影响     

肖明霞  白华毅  袁丽红  赵平  李琦华  PHOUTHAPANE Vanhnaseng  程志斌 《动物营养学报》2023,(4):2220-2229

本试验旨在研究凝结芽孢杆菌对热应激肉鸡生长性能及肠道消化与吸收功能的影响。选择320只28日龄健康爱拔益加（AA）肉公鸡,随机分为5个组,每组8个重复,每个重复8只。常温对照组（TC组）肉鸡饲养于（22±1）℃的常温环境,饲喂基础饲粮;热应激组（HS组）肉鸡饲养于（34±1）℃的高温环境,饲喂基础饲粮;热应激试验组肉鸡饲养于（34±1）℃的高温环境,分别饲喂在基础饲粮中添加0.2×107(HS-BC0.2组)、1.0×107(HS-BC1.0组)、5.0×107CFU/g(HS-BC5.0组)凝结芽孢杆菌的试验饲粮。试验期14 d。结果表明：与TC组相比,HS组的末重、平均日增重（ADG）、平均日采食量（ADFI）,干物质和粗蛋白质表观代谢率,空肠食糜总淀粉酶和总蛋白酶酶活,空肠绒毛高度、绒毛高度/隐窝深度以及空肠钠/葡萄糖协同转运蛋白1(SGLT1)、基础氨基酸转运载体（r-BAT）、y+L氨基酸转运载体-1(y+LAT1) mRNA表达量均显著降低（P<0.05）,料重比（F/G）显著增加（P<0.05）。与HS组相比,HS-BC1.0和HS-BC5.0组的ADG、粗蛋...  相似文献   

Sintering of Magnetite Concentrates Without Carbon     

REN Wei  BAI Chen-guang  QIU Gui-bao 《保鲜与加工》2006,(8):115-118

With the goal of the sintering of magnetite concentrates without carbon,the effect of quality,inert atmosphere and Fe_3O_4 addition on microwave sintering of magnetite concentrates is studied.It is shown that: With the same basicity(CaO/SiO_2=2.5) and microwave heating condition(1000W,2.45GHz,20min),the centre temperature decreaseds with a increasing sample mass;There is no great difference between with and without inert atmosphere in microwave sintering of magnetite in a same microwave heating condition.When 100g sample(CaO/SiO_2=2.5) is heated by microwave irradiation(1000W,2.45GHz,20min),the more Fe_3O_4 is added,the less liquid phase is produced,which is bad for microwave sintering of magnetite concentrate.  相似文献   

Reuolution and Research of Green Automobile     

RAN Zheng -y  TIAN Long  NI Ling  YANG Chao  CAO Wen -ming 《保鲜与加工》2002,(7):15-19

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.  相似文献   

Research of the Coefficient of Wind-induced Vibration About Complicated Tall Buildings     

ZHANG Liang-liang  LUO Sheng  YU Yang  WU Yun-fang  LIN Guan-ming  LIN Rong-sheng 《保鲜与加工》2006,(5):96-98

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THE APPLICATION OF SAND-GRAVEL-CUSHION TECHNIOUE IN THE FOUNDATION OF MOUNTAINOUS AREA     

Hu Daiuen  Huang Qiushun 《保鲜与加工》1995,(3):126-130

This paper presents the application of sand-gravel-cushion technique in thefoundation of mountainous area inhomogeneity and analyges non-uniformity of the foundation inmountainous,It is different from the traditional theory of sand-cushion,This is a new developmentand able to make a full use of the rock debris fill and the combinational soil-rock foundation in the mountainous areas.  相似文献   

A Muti-Objective Network Planning Model for Project     

Zen Fanqi Gu Yuasheng Zen Xianglin 《保鲜与加工》1993,(4):136-143

Based on the network planning model developed by the author for an overhaul project of a rolling mill production line in a heavy steel plant, an new multiobjective network planning model is proposed in this paper. The formulations of network time parameters are derived. The optimization problem of multiobjective network planning is also discussed. And it has been found out that the fractional single-objctive network is only one of the cases in this model.  相似文献   

高产优质商品玉米生产模式：—三种遗传效应集成利用（TEU）   总被引：3，自引：0，他引：3  

段民孝  宋同明等 《种子》2001,(2):3-6

通过大量详实资料，对玉米杂种优势、雄性不育，油分基因花粉直感等3种遗传效应的研究现状作了详细概述，根据相关的遗传理论与实践，提出了将三种遗传效应进行技术集成的可能性，从而形成一种高产高油玉米生产模式，有可能是未来玉米生产的发展方向。  相似文献   

Study on Genetic Fingerprinting Technique of Microbial Communities     

YE Jiang yu  LUO Gu yuan 《保鲜与加工》2003,(3):147-153

The 99% of all microorganisms in naturecannot be isolated by standard culture methods without conspicuous external characters to classify them morphologically. In addition, classification based on physiological or biochemical features are often fuzzy. Molecular techniques are used for uncultured microorganisms to explore the diversity of microbial communities and novel resources without the need of cultivation. DNA dynamics and cloning of PCR products obtained from environmental DNA is now routinely used to identify microbial populations in microbial ecology. For practical reasons the approach is not suited to study the complex dynamics of microbial communities in environment. Genetic fingerprinting techniques are better tools, which give complement for the traditional microbiological methods. The methods are rapid and relatively easy to perform, and they allow the simultaneous analysis of multiple samples, which makes it possible to compare the genetic diversity of microbial communities from different habitats, or to study the behaviour of individual communities over time.  相似文献