基于简化基因组测序技术的甘薯HRM分子标记开发及其应用     

冯俊彦  郎涛  张聪  李明  青莉芳  屈会娟  蒲志刚  康乐 《核农学报》2022,36(7):1350-1361

目前甘薯中针对SNP位点的分子标记开发及应用的报道较少。为开发甘薯特异SNP分子标记,本研究利用简化基因组测序技术对甘薯种质材料进行测序,筛选稳定的SNP位点,将其开发为基于HRM技术的分子标记,并在甘薯种质材料中进行验证。通过对23个甘薯种质材料简化基因组测序数据的分析,共发现835 756个SNP多态性位点,筛选其中的3 650个含有SNP多态性位点的高质量测序片段,成功设计了134对引物;初步验证发现,22对(16.42%)引物没有扩增产物,15对(11.19%)引物扩增产物2个以上,36对(26.87%)引物的扩增产物没有差异。61对(45.52%)引物在8个样本中的扩增特异性好,而且样本之间有差异。最后筛选34对扩增多态性丰富的引物对52份甘薯种质材料进行扫描,发现材料间多态性介于27.27%~90.91%之间,平均多态性达到59.35%。聚类分析表明,参试52份甘薯种质材料之间的差异较小,多数材料与骨干亲本南瑞苕、徐薯18之间关系较近,国外引进甘薯材料和地方品种差异较大。建议在今后甘薯育种中尽量选用地方品种和国外甘薯品种,从而更好地拓宽甘薯遗传背景。本研究初步建立了基于简化基因组技术和HRM技术开发甘薯SNP分子标记的新思路,为今后甘薯SNP分子标记开发提供了参考。同时本研究开发的甘薯分子标记,丰富了甘薯分子标记类型,为甘薯研究者开展快速的分子标记研究提供了支持。  相似文献   

气候变化与土壤湿度关系的研究进展   总被引：4，自引：0，他引：4  

林洁  陈效民  张勇 《土壤通报》2012,(5):1271-1276

在分析气候变化与土壤湿度关系研究现状的基础上,综述了该研究的进展。首先,描述了用数值模拟方法研究气候变化与土壤湿度之间关系的现状;论述了该研究取得的成果和需要进一步研究的问题;其次,简要总结了目前研究仍显薄弱的资料诊断分析工作及取得的成果。土壤湿度的监测与预报是制定合理的灌溉制度从而实现适时适量灌溉的基础,因此本文对气候变化条件下土壤湿度变化趋势的研究及节水灌溉(特别是干旱地区)具有重要的意义。  相似文献   

农作物空间格局变化模拟模型的MATLAB实现及应用   总被引：2，自引：4，他引：2  

余强毅  吴文斌  陈羊阳  杨鹏  孟超英  周清波  唐华俊 《农业工程学报》2014,30(12):105-114

Agent模型是研究农业土地系统复杂性与动态性的有效工具。在农作物空间格局变化模拟模型(CroPaDy,an agent-based model for simulating crop pattern dynamics)概念化设计的基础上,借助MATLAB平台开放性、矩阵运算能力强等特点,实现CroPaDy模型的数值模拟,并以黑龙江省宾县调查数据为依据,完成模型的区域实证研究。基于MATLAB的模型实现过程充分考虑了CroPaDy模型的多层次性(土地流转行为与作物选择行为)成功实现了3个子模块的动态嵌套模拟:1)Agent生成模块。基于已有的多源GIS数据、统计数据、典型调查数据、以及个体的通用规则,利用蒙特卡洛方法生成每一个个体Agent的属性信息;2)Agent分类模块。基于调查数据对受访农户进行态度聚类分析,然后借助人工神经网络方法确定所有生成的Agent所在的类型;3)Agent决策模块。利用概率方法,计算特定周期内每个Agent的决策行为。区域实证研究中,直接将空间耕地网格作为个体Agent,实现区域全覆盖(网格大小设置为114 m×114 m,约等于户均耕地面积),模拟结果表明,研究区2010年玉米、大豆、水稻、烤烟的模拟结果分别为2 6055.9、5 192.2、3 506.8、3 983.9 hm2,利用宾县统计年鉴(2010)进行验证,模型总体模拟精度达90%以上。CroPaDy模型的设计与实现科学合理,具有较强的理论性与可操作性,能够用以表达特定区域内的农作物空间格局及其动态变化过程。  相似文献   

环模制粒粉体旋转挤压成型扭矩模型构建及试验     

武凯  孙宇  彭斌彬  丁武学  王栓虎 《农业工程学报》2013,29(24):33-39

环模制粒成型技术以其高效率、高成型率、低污染等优点广泛应用于饲料机械、生物质能源机械、化工、制药等领域,而环模制粒过程扭矩模型的缺失已成为制约制粒技术发展的瓶颈之一。该文旨在通过理论分析、数值模拟与试验研究,建立粉体旋转挤压制粒成型过程精确扭矩模型,为粉体旋转挤压制粒成型装备的节能降耗及优化设计奠定理论基础。针对各向同性粉体物料,基于微单元受力分析及广义胡克定律,建立了旋转挤压制粒成型过程中模孔不同深度位置挤压压强的计算公式;基于DPC模型及实际辊轧工艺,建立了旋转挤压成型过程有限元分析模型,进行了压紧区应力分析;基于有限元模拟分析结果,设定变形压紧区的压强分布为二次曲线,建立了变形压紧区、挤压区的压强分布模型;最后建立了粉体旋转挤压制粒成型扭矩模型。以无线扭矩测试系统及环模制粒机为试验平台进行了鸡饲料的环模挤压制粒试验,求解得到了所有模型常量。设计了9组扭矩测试试验进行模型验证,测试结果与理论计算对比表明,试验值与计算值差距较小,最大计算误差仅为2.6%,这反映出建立的模型正确有效,对指导环模制粒机结构优化与节能降耗具有重要的意义。  相似文献   

基于语义驱动的日光温室图纸三维建模方法     

杜建军  郭新宇  陆声链  温维亮  肖伯祥 《农业工程学报》2013,29(25):190-196

为了从日光温室施工图纸中重建出温室结构三维模型,提出基于语义映射的施工图纸三维重建方法。首先解析和分类日光温室施工图纸DXF文件,从中识别和理解主要特征构件,建立日光温室构件语义模型并生成分层控制参数,进而基于参数化驱动日光温室三维建模,最后对日光温室三维模型进行真实感渲染。该方法整合CAD制图与三维建模技术,有利于提高日光温室施工图纸的读图效率,建立的三维温室模型具有保真性、交互性和真实感等特性,可用于工程量和造价计算、温室结构优化以及虚拟展示。  相似文献   

Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea     

Saiful Karim 《Soil Science and Plant Nutrition》2013,59(6):827-839

To separate soil humic acids (HAs) into their constituents and characterize them, polyacrylamide gel electrophoresis (PAGE) was carried out in the presence of 7 M urea using a preparative electrophoresis system. Two types of soil HAs were fractionated into nine fractions by PAGE. The dark-colored constituents were recovered from the electrophoretic fractions by precipitation on acidification, and the brown-colored constituents dissolved in the acidic solution of fast-moving fractions were recovered by adsorption onto DAX-8 resin. High-performance size-exclusion chromatography (HPSEC) confirmed that the constituents of the HAs were separated based on their molecular size by PAGE. The dark-colored constituents exhibited higher degrees of humification than did the corresponding unfractionated HAs, except for the constituents remaining in the electrophoretic gels at the end of electrophoresis. Diffuse reflectance infrared spectroscopy revealed that the chemical properties of the dark-colored constituents changed regularly: the content of carboxyl groups decreased and the proportions of proteinous, aliphatic and polysaccharide moieties increased with increasing molecular size. The humification degrees of the constituents adsorbed onto DAX-8 resin were considerably lower than those of the corresponding unfractionated HAs. The chemical properties of the DAX-8-adsorbed constituents were different from those of the dark-colored constituents. Observation of electrophoretic fractions under blue light (470 nm) and HPSEC with fluorescence detection at an excitation wavelength of 460 nm and an emission wavelength of 520 nm showed that green fluorescent substances were largely concentrated in the smallest molecular size fractions and were partitioned into both the dark-colored precipitates and DAX-8-adsorbed fractions. The proportion of organic carbon recovered by precipitation and adsorption onto DAX-8 resin was 45–63%, indicating that substantial parts of the HA constituents were missing. The unrecovered constituents were considered to be acid-soluble, nearly colorless substances. The dissociation of the acid-soluble constituents from the acid-insoluble dark-colored constituents during the preparative PAGE of soil HAs was ascribed to disruption of hydrogen bonding and hydrophobic interactions caused by concentrated urea.  相似文献   

基于Modelica语言建模的柴油机稳动态性能仿真与试验     

李文辉  刘长铖  马修真  夏文  张子鉴 《农业工程学报》2016,32(21):87-94

针对传统柴油机建模效率不高、所建模型抽象不易理解、软件平台开放程度差、模型通用性低的问题,采用多领域统一物理建模规范Modelica语言和面向对象的建模思想建立了柴油机的仿真模型。以上柴D4114B型发电用柴油机为例,在解决上述问题的同时模拟了负荷特性下柴油机的稳态过程,最高压力、排温等稳态数据点的实测值与仿真值的最大相对误差为8.9%,证明了所建立的柴油机模型具有较好的稳态性能。在此基础上进行了动态仿真,模拟了转速、单缸循环油量等参数的动态变化规律,并将部分参数的仿真值与实测值进行比较,两者吻合得比较好,证明了所建立的柴油机模型能较好地预测其动态性能,可为后续的基于Modelica语言的柴油机建模提供参考。  相似文献   

The use of lysimeter data for the test of two soil–water balance models: A case study     

Martin Wegehenkel  Yongqian Zhang  Thomas Zenker  Heiko Diestel 《植物养料与土壤学杂志》2008,171(5):762-776

Two soil–water balance models were tested by a comparison of simulated with measured daily rates of actual evapotranspiration, soil water storage, groundwater recharge, and capillary rise. These rates were obtained from twelve weighable lysimeters with three different soils and two different lower boundary conditions for the time period from January 1, 1996 to December 31, 1998. In that period, grass vegetation was grown on all lysimeters. These lysimeters are located in Berlin‐Dahlem, Germany. One model calculated the soil water balance using the Richards equation. The other one used a capacitance approach. Both models used the same modified Penman formula for the estimation of potential evapotranspiration and the same simple empirical vegetation model for the calculation of transpiration, interception, and evaporation. The comparisons of simulated with measured model outputs were analyzed using the modeling‐efficiency index IA and the root mean squared error RMSE. At some lysimeters, the uncalibrated application of both models led to an underestimation of cumulative and annual rates of groundwater recharge and capillary rise, despite a good simulation quality in terms of IA and RMSE. A calibration of soil‐hydraulic and vegetation parameters such as maximum rooting depth resulted in a better fit between simulated and observed cumulative and annual rates of groundwater recharge and capillary rise, but in some cases also decreased the simulation quality of both models in terms of IA and RMSE. The results of this calibration indicated that, in addition to a precise determination of the soil water‐retention functions, vegetation parameters such as rooting depth should also be observed. Without such information, the rooting depth is a calibration parameter. However, in some cases, the uncalibrated application of both models also led to an acceptable fit between measured and simulated model outputs.  相似文献   

低分子有机酸对土壤中重金属的解吸及影响因素   总被引：9，自引：1，他引：9  

MAYun-long  ZENGQing-ru  HUHao  PANJie  LIUXiao-yan 《土壤通报》2008,(6)

研究了柠檬酸、草酸、酒石酸和苹果酸对矿区土壤中重金属Pb、Cd、Cu和Zn的解吸行为,并探讨了介质pH值对其解吸土中重金属的影响。振荡解吸试验结果表明四种低分子有机酸对供试污染土壤中Pb、Cd、Cu和Zn都具有一定的解吸能力。由于土壤中重金属有效态含量较低,各重金属的解吸率都不高。在对Pb和Cd的解吸中,各低分子有机酸能力大小顺序为柠檬酸>酒石酸≈苹果酸>草酸;Cu的解吸顺序为柠檬酸>草酸>酒石酸≈苹果酸;Zn的解吸顺序为酒石酸>柠檬酸≈苹果酸>草酸。低分子有机酸随浓度的增加,其解吸能力提高。低分子有机酸对重金属的解吸量随pH值的降低而增加。  相似文献   

DEM栅格分辨率对多元线性土壤—景观模型及其制图应用的影响   总被引：6，自引：1，他引：6  

孙孝林  赵玉国  秦承志  李德成  赵量  张甘霖 《土壤学报》2008,45(5):971-977

以亚热带丘陵地区为对象,以该区4 km×3 km的5 m、10 m、15 m、20 m、25 m、30 m数字高程模型(DEM)为基础,建立多元线性土壤景观模型,并应用该模型预测研究区内土壤表层有机质含量分布,进而比较不同分辨率DEM中土壤景观模型及其预测制图的精度。结果表明:在本研究区11 km2范围内,随着DEM栅格分辨率降低,坡度、曲率、比汇水面积(对数)频度均表现出了向其中值区集中的趋势;地形因子的这一变化规律对土壤景观模型的影响较小,例如模型的变量、变量系数及R2在不同分辨率DEM中的差异很小;但地形因子的这一变化规律对模型预测制图的精度具有较大影响,各项指标均说明,模型在10~25 m DEM中的制图精度较高,而在更高分辨率(5 m)或更低分辨率(30 m)DEM中较低。本研究结果对其他亚热带丘陵地区具有一定的指示意义。  相似文献