Mitochondrial membrane potential and caspase-3 are involved in apoptosis of RD cells induced by TRAIL and cisplatin     

XU Yu-sheng  MIAO Jin-hong  WANG Li-min  WANG Yi-sheng  XU Jian-zhong  YANG Di-sheng 《园艺学报》2007,23(1):142-145

AIM: To study the synergistic induction of apoptosis by TRAIL and cisplatin in rhabdomyosarcoma cells and investigate the role of mitochondrial membrane potential and caspase-3 in this process.METHODS: Rhabdomyosarcoma cells were treated with TRAIL, cisplatin for 3 days, respectively or combination. The cytotoxicity was observed by MTT assay. The apoptotic rates and change of mitochondrial membrane potential and caspase-3 were determined by flow cytometry (FCM). The obvious morphological changes in rhabdomyosarcoma cells were confirmed by electron microscope. RESULTS: Rhabdomyosarcome cells were treated with TRAIL (1.0, 10.0 and 100.0 μg/L), the cytotoxicity indices were 18.9%, 20.8% and 43.5%, respectively. With cisplatin (1.0, 5.0 and 10.0 mg/L), the indices of cytotoxicity were 9.8%, 23.4% and 43.8%, respectively. When TRAIL and cisplatin treatment used simultaneously, the cytotoxicity index increased obviously. The activity of caspase-3 in rhabdomyosarcoma cells was upregulated and the mitochondrial membrane potential was downregulated with cisplatin, which were paralleled by the apoptotic rates. The obvious apotosis morphological changes in rhabdomyosarcoma cells were shown by electron microscope. CONCLUSION: TRAIL and cisplatin are able to kill rhabdomyosarcoma cells. TRAIL in combination with cisplatin shows synergistic effect on rhabdomyosarcoma cells by increasing the caspase-3 activity and suppressing mitochondrial membrane potential.  相似文献   

The Expression Pattern and Ligand Binding Ability of Hc-FAR-4 Protein of Haemonchus contortus     

WEI HaiDian  CHEN XueQiu  HUANG Yan  SHI HengZhi  ZHOU JingRu  WU Fei  DU AiFang  YANG Yi 《中国农业科学》2019,52(17):3059-3068

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Isolation,properties and induction of plaice liver cytosolic glutathione-S-transferases     

Stephen G. George  Gordon Buchanan 《Fish physiology and biochemistry》1990,8(6):437-449

Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to rat ligandin, however, bilirubin was only bound weakly and the enzymes did not display the inhibition characteristics of ligandin. They were extremely sensitive to inhibition by organotin compounds. Plaice transferase A was a homodimer of Mr 27 kDa subunits; it displayed strongest activity with CDNB and some activity with DCNB as substrates. Specific antibodies to it did not show any relationship with other plaice GST's or rat GST subunits 1,2,3,4,7,8 or 10. Plaice transferase B was a homodimer of Mr 25 kDa subunits, it displayed similar activities with CDNB and DCNB as substrates to transferase A and a low activity with ethacrynic acid or p-nitrobenzylchloride. It was immunologically related to the rat alpha class transferase subunits 1,2 and 8. A minor form, transferase AM, which was not separated from transferase A, appeared to be a heterodimer of Mr 25.5 and 27 kDa subunits. It displayed a greater activity with DCNB than the other plaice GST's and some activity with bromosulphalein indicative of a possible relationship with the Mu class transferases.  相似文献   

Plumbagin enhances TRAIL-induced apoptosis in Kasumi-1 cells     

XU Tong-peng  LIU Li-gen  JIANG Xue-wei  GAO Wu  XIE Ying-hua  ZHAO Li-min  HAN Xi-yao  LU Dao-pei 《园艺学报》2011,27(3):481-487

AIM: To investigate the effect of plumbagin and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the apoptosis of leukemic Kasumi-1 cells. METHODS: Kasumi-1 cells were treated with plumbagin alone, recombinant soluble TRAIL(rsTRAIL) alone or the combination of plumbagin with rsTRAIL to induce apoptosis. The cell proliferation was analyzed by CCK-8 assay. Apoptosis was determined by flow cytometry with AnnexinⅤ/PI double staining and TUNEL staining. The expression of DR4 and DR5 at mRNA level was measured by real-time PCR. The expression of signal transduction proteins, such as DR5, caspase-3, caspase-8, caspasep-9, Bid, Bax and c-FLIP was detected by Western blotting. RESULTS: Both rsTRAIL and plumbagin induced the apoptosis in Kasumi-1 cells, and combination of plumbagin with rsTRAIL enhanced the apoptosis. The ratios of Annexin V-positive Kasumi-1 cells were (27.7±2.9)%, (25.6±3.1)% and (52.1±3.3)% in 100 μg/L rsTRAIL group, 2 μmol/L plumbagin group and the combination group, respectively, and the positive rate in combination group was significantly higher than those in other 2 groups. TUNEL assay demonstrated that the number of apoptotic cells in combination group was higher than that in the cells treated with rsTRAIL or plumbagin alone. Plumbagin up-regulated the expression of DR5 at mRNA level in Kasumi-1 cells, and up-regulation of DR5, activation of caspase-8 and down-regulation of c-FLIP at protein level were detected in the cells treated with plumbagin alone and the combination of plumbagin with rsTRAIL. CONCLUSION: Plumbagin enhances TRAIL-induced apoptosis in Kasumi-1 cells by up-regulating DR5, activating caspase-8 and down-regulating c-FLIP.  相似文献   

Change of serum CXC chemokine ligand 16 in gout patients and its clinical significance     

WU Fan  GONG Qi  YU Jia-wen  LU Ting-ting  LI Xiao-kun  LIN Zhuo-feng 《园艺学报》2011,27(10):1977-1981

AIM: To explore the relationship between the changes of serum CXC chemokine ligand 16 (CXCL16) and the loss of kidney functions in chronic gout patients and its clinical significance. METHODS: Twenty gout patients with chronic kidney disease (CKD), 22 gout patients without CKD and 22 CKD subjects were recruited into the present study, while 20 normal age-and sex-matched subjects were assigned into the control group. Serum level of CXCL16 and other relevant clinical and biochemical parameters in all subjects were obtained upon standard clinical examinations. Ceatinine clearance rate (CCR) and estimated glomerular filtration rate (eGFR) were calculated based on the clinical parameters. To analyze the clinical data, student's unpaired t-test was used for the comparison between 2 groups. One-way ANOVA assay and multiple stepwise regression were used for multiple groups.RESULTS: Serum level of CXCL16 was significantly increased in gout subjects compared with the healthy control and CKD subjects (P<0.05). Serum level of CXCL16 in gout patients with CKD was significantly higher than that in gout patients without CKD (P<0.05). Furthermore, the mRNA expression of CXCL16 in peripheral blood mononuclear cells (PBMC) of gout patients was significantly higher than that in healthy subjects. Multiple stepwise regression analysis indicated that serum CXCL16 was independently associated with 24 h urine protein, CCR and C-reactive protein (P<0.05). CONCLUSION: Serum CXCL16 level in gout patients is associated with the change of renal functions. Elucidating the pathophysiologcial mechanism of CXCL16 in gout patients requires further study.  相似文献   

Injury effect of recombinant soluble human CD40 ligand on human umbilical vein endothelial cells     

LIU Hong-li  CHEN Meng  WANG Hong-tao  WU Yi-ling 《园艺学报》2015,31(6):1111-1114

AIM: To investigate the damage in human umbilical vein endothelial cells (HUVECs) induced by recombinant soluble human CD40 ligand (rshCD40L). METHODS: The cultured HUVECs were treated with rshCD40L for 12 h. The survival activity of the HUVECs was observed by MTS assay. The expression of E-selectin, intercellular adhesion molecule (ICAM)-1, tissue factor (TF) and tissue factor pathway inhibitor (TFPI) was measured by ELISA. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were detected by the methods of thibabituric acid (TBA). RESULTS: Compared with normal group, different concentrations of rshCD40L (0.5, 1, 2, 3 mg/L) had no obvious effect on the survival activity of the HUVECs (P>0.05). rshCD40L at concentration of 0.5 mg/L promoted the secretion of E-selectin, sICAM-1, TF and TFPI in the HUVECs (P<0.01). rshCD40L at concentration of 0.5 mg/L also increased MDA content and reduced the activity of SOD in the HUVECs (P<0.05). CONCLUSION: 0.5~3mg/L rshCD40L has no obvious effect on endothelial cell survival, but already causes endothelial dysfunction by increasing endothelial inflammation and exogenous coagulation reaction, inducing lipid peroxides injury and reducing antioxidant capacity.  相似文献   

Studies on the maturing process of newly reclaimed soils under cultivation (Part 5) Change of the methoxyl content and the heat of combustion of soil humus under cultivation     

Kenzo KōBō 《Soil Science and Plant Nutrition》2013,59(1):91-93

It has been considered by investigators, no matter whether they stand by the lignin-protein complex theory or not, that lignin plays an important role in the humus formation of plant residues. When plant residues are added to the soil, though almost all the plant constituents are decomposed, only lignin accumulates in the soil as a component of humus, as it is resistant to the microbial decomposition. However, lignin does not remain unchanged in this case and is thought to be modified, either through chemical agencies or as a result of specific microbial action. Though it is not yet fully clarified what changes it undergoes in the humification process, at least it is indicated that the methoxyl, as a characteristic radical of lignin, is attacked during the process.  相似文献   

Sorption behavior of selenium and antimony in soils as a function of phosphate ion concentration     

《Soil Science and Plant Nutrition》2013,59(3):332-341

Abstract

Both selenium (Se) and antimony (Sb) are major soil and water pollutants. Their sorption behavior in a soil–plant system was studied. Soil–soil solution distribution coefficients (K _ds) for Se and Sb were measured, using a radiotracer, as an indicator of their sorption levels. Both Se and Sb behave as oxoanions (SeO^2? ₄, H₂PO^? ₄ and SO^2? ₄) in soil; thus, the effects of concentrations of two major oxoanions (SeO^2? ₄ and SeO^2? ₃) on Se and Sb sorption were also examined. The K _d values for Se for Japanese soils significantly correlated with the K _d values for Sb (n = 141). The K _ds of both Se and Sb similarly decreased with increasing SbO^? ₃ concentration. These results indicated that the sorption of Se and Sb was similarly controlled by a ligand-exchange mechanism such as phosphate sorption in soil. However, an increase in the concentration of SeO^2? ₃ did not decrease the K _ds of Se and Sb. Furthermore, the ligand-exchangeable fractions of stable Se and Sb in major Japanese soils were determined by extraction with 0.1 mol L^?1 Na₂HPO₄ solution. For both Se and Sb, the phosphate-extractable fractions were 10-fold higher for Se and fivefold higher for Sb than their water-soluble fractions. Although the total Se and Sb amounts in soils were the same, their ligand-exchangeable fractions were different. Approximately 0.9–12% of total Se and 0.2–1.3% of total Sb were extracted by the phosphate solution. These findings suggested that Se was more likely to be mobilized by the addition of phosphate than Sb. The effect of plant-available phosphate in the soil and the phosphate sorption capacity of soil on Se and Sb availabilities for plants were also examined using a pot experiment with soybean plants. The experimental results suggested that a high content of available phosphate and/or low phosphate sorption capacity of soil increased both Se and Sb availabilities to the plant. However, the results also suggested that the soil Se availability to the plant was higher than that of Sb even though the soil total Se and Sb amounts were the same.  相似文献   

体外TLR3配体增强鸭坦布苏病毒灭活苗效果的免疫机制研究     

提金凤  李志杰  王海燕  王莉莉 《中国动物传染病学报》2022,(1):20-29

为了阐明TLR3配体对鸭坦布苏病毒(DTMUV)灭活苗的免疫增强作用,以TLR3配体poly(I:C)和灭活DTMUV作用雏鸭外周血单个核细胞(PMBC),通过体外检测TLR3相关信号蛋白和细胞因子转录及表达水平,明确TLR3配体发挥免疫增强作用的主要信号通路.制备雏鸭PMBC,选择TLR3配体poly(I:C)与灭活...  相似文献   

2,2’-联吡啶基嘧啶桥连Cr配合物的电子结构及光谱性质的理论研究     

刘畅  姜松  邹宪芝  赵桂云 《安徽农业科学》2013,(25):10224-10225,10231

以密度泛函和含时密度泛函为理论基础,通过Gaussion03程序,应用密度泛函中的B3LYP方法,在6-31G（d）基组下,对2,2’-联吡啶基嘧啶桥连Cr配合物的电子结构及光谱性质进行了系统的量子化学理论研究.同时,比较了配合物的几何构型、红外光谱和电子吸收光谱.该研究对有关联吡啶基嘧啶桥连Cr配合物的开发与利用有一定的促进作用.  相似文献