Therapeutic effects of modified Bushen Huoxue granules on ovariectomized osteoporosis rats     

HUI Hong-yan  DONG Yu-zhen 《园艺学报》2016,32(9):1683-1687

AIM: To observe the therapeutic effect of modified Bushen Huoxue granules (MBHG) on ovariectomized osteoporosis rats. METHODS: Female SD rats were randomly given sham operation (n=10) and ovariectomy, and then the model rats were further randomly divided into model group, MBHG treatment groups at doses of 200 mg/kg, 100 mg/kg and 50 mg/kg respectively, and positive control (estradiol valerate) group, with 10 rats in each group by intragastric administration for 12 weeks. The morphology, area, thickness, spacing and area percentage of trabecular bone in the rats were observed. The serum levels of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) were mea-sured by automatic analyzer. Bone mineral density (BMD) was analyzed. Serum estradiol (E₂), osteocalcin (BGP), osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANK) and receptor activator of nuclear factor κB ligand (RANKL) levels were detected by ELISA. RESULTS: Compared with model group, trabecular bone significantly widened in all treatment groups with large number, and net-like structure restored partially. The thickness, area and area percentages of trabecular bone in treatments groups were higher than those in model group,and trabecular spacing was less than that in model group (P<0.05). The serum Ca, P, E₂ and OPG, and femoral BMD were significantly higher in treatment groups than those in model group, and the levels of ALP, BGP, RANK and RANKL were significantly lower than those in model group (P<0.01).CONCLUSION: MBHG has a significant therapeutic effect on ovariectomized osteoporosis rats. The mechanism may be related to the regulation of OPG and inhibition of RANKL secretion.  相似文献   

Tumor necrosis factor-related apoptosis-inducing ligand participates in injury of vascular endothelial cells induced by rapamycin in vitro     

YE Sheng  ZHANG Huai-qin  LIN Yi-nuo  HUANG Wei-jian  ZHANG Yan-li  SHAO Xiao-lin 《园艺学报》2011,27(2):254-259

AIM: To investigate the effects of rapamycin on apoptosis, proliferation, migration ability and tumor related apoptosis inducing ligand(TRAIL) in cultured human umbilical vein endothelial cells(HUVECs). METHODS: Cultured HUVECs were treated with rapamycin at the concentrations of 0, 1, 10 and 100 μg/L for 24 h. The cell proliferation was measured by CCK-8 method. The cell migration ability was detected by Transwell chambers and wound healing test. The apoptotic index of HUVECs was quantitatively determined by measuring the activation of caspase-3. The morphological changes of the apoptotic cells were observed by DAPI staining. The expression of TRAIL was detected by Western blotting. RESULTS: A 24 h-incubation with rapamycin(1-100 μg/L) caused significant cell loss associated with the increase in apoptosis, as quantified by the determination of caspase-3 activity(P<0.01) in HUVECs. Obvious apoptotic morphology was observed by DAPI staining in HUVECs incubated with rapamycin. Rapamycin at the concentrations of 1-100 μg/L also impaired the migration ability of HUVECs(P<0.01). In addition, rapamycin(10-100 μg/L) inhibited the proliferation of HUVECs, whereas rapamycin at 1 μg/L had no such effect(P<0.01). Rapamycin(10-100 μg/L) also induced TRAIL expression in a dose-dependent manner(P<0.01). CONCLUSION: Rapamycin induces apoptosis, and inhibits the proliferation and migration of HUVECs. The up-regulation of TRAIL might be related to the injury of vascular endothelial cells caused by rapamycin.  相似文献   

Imperatorin enhances anti-tumor effect of TRAIL on breast cancer by upregulating expression of DR5 on cell surface     

XU Zheng-yang  REN Rui-ping  WAN Peng  YUAN Zu-guo 《园艺学报》2018,34(1):81-86

AIM: To investigate the synergistic effect of imperatorin on enhancing the anti-tumor effect of TNF-related apoptosis-inducing ligand (TRAIL) on breast cancer and the mechanisms. METHODS: T-47D and MCF-7 breast cancer cells were divided into control group, imperatorin group, TRAIL group, imperatorin+TRAIL group and imperatorin+TRAIL+death receptor 5 (DR5) siRNA group. The viability of T-47D and MCF-7 cells was measured by MTT assay. The apoptosis and mitochondrial membrane potential in T-47D cells were analyzed by flow cytometry. Western blot and flow cytometry analysis were performed to evaluate the expression of DR5 on T-47D cell surface and the activation of caspase-8 and caspase-3. RESULTS: Imperatorin significantly enhanced the inhibition of cell viability induced by TRAIL of T-47D and MCF-7 cells, and significantly increased the apoptosis of T-47D cells induced by TRAIL. Imperatorin treatment ob-viously induced upregulation of DR5 expression and production of reactive oxygen species in the T-47D cells. In addition, imperatorin enhanced the TRAIL-induced damage of mitochondrial membrane potential and activation of caspase-8 and caspase-3. CONCLUSION: Imperatorin enhances the anti-tumor effect of TRAIL on breast cancer via upregulating the expression of DR5.  相似文献   

Regulation of granulosa cell apoptosis by death ligand–receptor signaling     

Fuko MATSUDA-MINEHATA  Akihisa MAEDA  Yuan CHENG  Takafumi SAI  Hiroshi GONDA  Yasufumi GOTO  Noboru MANABE 《Animal Science Journal》2008,79(1):1-10

Several hundred thousand primordial follicles are present in the mammalian ovary, however, only 1% develop to the preovulatory stage and finally ovulate. The remainder will be eliminated via a degenerative process called ‘atresia’. The endocrinological regulatory mechanisms involved in follicular development and atresia have largely been characterized but the precise temporal and molecular mechanisms involved in the regulation of these events remain unknown. Many recent studies suggest that apoptosis in ovarian granulosa cells plays a crucial role in follicular atresia. Notably, death ligand‐receptor interaction and subsequent intracellular signaling have been demonstrated to be the key mechanisms regulating granulosa cell apoptosis. In this review we provide an overview of granulosa cell apoptosis regulated by death ligand‐receptor signaling. The roles of death ligands and receptors [Fas ligand (FasL)]‐Fas, tumor necrosis factor α (TNFα)‐TNF receptor and TNFα‐related apoptosis‐inducing ligand (TRAIL)‐TRAIL receptor (TRAILR)] and intracellular death‐signal mediating molecules (Fas‐associated death domain protein), TNF receptor 1‐associated death domain protein, caspases, apoptotic protease‐activating factor 1, TNFR‐associated factor 2 and cellular FLICE‐like inhibitory protein in granulosa cells are discussed.  相似文献   

Effect of TAP-SSL5 fusion protein on binding of activated platelets to human lymphocytes     

PENG Song  BEI Jun-jie  HU Hou-yuan  CHEN Qiang 《园艺学报》2015,31(1):23-27

AIM: To study the effect of tick anticoagulant peptide-staphylococcal superantigen like protein 5 (TAP-SSL5), an anti-inflammatory and anticoagulant fusion protein, on the binding of activated platelets to human lymphocytes.METHODS: Human periphery lymphocytes were isolated by magnetic activated cell sorting (MACS). The toxicity of TAP-SSL5 on the viability of Jurkat cell was assessed by CCK-8 assay. Flow cytometry was applied to detect the expression of CD162 (PSGL-1) on the Jurkat cells (human peripheral blood leukemia T lymphocyte cell line) and the inhibitory effect of TAP-SSL5 on the binding of mouse anti-human CD162 monoclonal antibody (KPL-1) to Jurkat cells. Platelets were activated by ADP at concentration of 20 μmol/L, the binding rates of activated platelets to Jurkat cells or human lymphocytes were assayed by flow cytometry. RESULTS: The concentration of TAP-SSL5 below 30 mg/L didn't affect the viability of Jurkat cells. TAP-SSL5 at 10 mg/L competitively inhibited KPL-1 binding to Jurkat cells. The binding rates of activated platelets to Jurkat cells or lymphocytes were (11.86±4.49)% and (8.32±1.00)%, respectively, which decreased to (6.73±2.71)% and (5.51±0.70)% after the Jurkat cells and lymphocytes were pre-incubated with 10 mg/L TAP-SSL5 (P <0.05).CONCLUSION: TAP-SSL5 binds to PSGL-1 expressed on lymphocyte surface and directly inhibits the binding of activated platelets to human lymphocytes, which may be one of the anti-inflammatory mechanisms of TAP-SSL5.  相似文献   

Effects of LXJD prescription,a Chinese medicine,on CCL20 and CCR6 expression in mouse skin with psoriasis     

WANG Ying  DI Ting-ting  RUAN Zhi-tong  ZHAO Jing-xia  LI Ping 《园艺学报》2015,31(2):331-336

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配位体交换在减慢由酸雨造成的可变电荷土壤酸化速度中的意义     

WANG Jing-Hu  YU Tian-Ren 《土壤圈》1996,6(1):11-22

For the purpose of evaluating the role of ligand exchange of sulfate ions in retarding the rate of acidification of variable charge soils,the changes in pH after the addition of different amounts of HNO3 or H2SO4 to representative soils of China were measured .A difference between pH changes caused by the two kinds of acids was observed only for variable charge soils and kaolinite,but not for constant charge soils and bentonite,The larger the proportion of H2SO4 in the HNO3-H2SO4 mixture,the lower the calculated H^ ion activities remained in the suspension.The difference in H^ ion activities between H2SO4 systems and HNO3 systems was larger for soils with a low base-saturation(BS) percentage than those with a high BS percentage.The removal of free iron oxides from the soil led to a decrease in the difference,while the coating of Fe2O3 on a bentonite resulted in a remarkable appearance of the difference.The effect of ligand exchange on the acidity status of the soil varied with the soil type.Surface soils with a high organic matter content showed a less pronounced effect of ligand exchange than subsoils did.It was estimated that when acid rain chiefly containing H2SO4 was deposited on variable charge soils the acidification rate might be slower by 20%-40% than that when the acid rain chiefly contained HNO3 for soils with a high organic matter content,and that the rate might be half of that caused by HNO3 for soils with a low organic matter content,especially for latosols.  相似文献   

砷硒胁迫对小麦毒性效应及预测模型研究   总被引：2，自引：1，他引：1  

李建秋  龚冰  季节  周子琛  仇浩 《农业环境科学学报》2020,39(2):239-246

为阐明含氧阴离子型类金属As-Se的联合毒性及其相互作用,以小麦(TriticumaestivumL.)作为模式植物,溶液体系为毒性测试介质,以小麦相对根伸长为毒性终点,系统考察了As、Se单独和复合情况下对小麦的毒性效应,并构建兼具机理性与普适性的模型来预测和评估As-Se的生态毒性效应和风险。结果表明:As、Se单独作用于小麦时,二者都具毒性,基于自由离子活度的EC50值分别为2.88μmol L-1和43.51μmol L-1,说明As的毒性远大于Se。在As-Se混合体系中,不论是何种剂量表达形式(溶解浓度和自由离子活度剂量),一种阴离子金属的存在都会影响另一种阴离子金属的植物毒性。利用传统浓度加和及独立作用(CA/IA)模型分析和预测As-Se联合毒性作用时,两种模型均高估了其联合毒性,表明As-Se联合作用于小麦时表现为强烈的拮抗作用。进一步将As-Se交互作用纳入在内,构建的阴离子型金属生物配体模型(BLM),可以很好地解释并预测As-Se混合物的交互作用及毒性,拟合度达到0.90,As、Se和生物配体的络合平衡常数分别为logKAsBL=3.28和logKSeBL=1.93。研究表明:BLM框架可拓展应用于含氧阴离子型类金属的毒性预测,为含氧阴离子型类金属联合毒性的精确预测与风险评估提供有效手段。  相似文献   

Molecular mechanism of BMSC intracerebral transplantation in improving learning and memory abilities of AD mice     

FAN Chong-zhu  LI An  HUANG Cui-qin  GAN Dan-hui  LI Qin  ZHAO Jia-yi  WANG Zhen  ZHU Li-hong  LU Da-xiang 《园艺学报》2017,33(11):1921-1931

AIM: To investigate the effect of bone marrow mesenchymal stem cell (BMSC) transplantation on learning and memory abilities and pathological changes of Alzheimer disease (AD) mice and the molecular mechanisms. METHODS: C57/BL6 wild-type (WT) and transgenic (Tg) mice were randomly divided into 4 groups:WT/PBS group, WT/BMSCs group, Tg/PBS group and Tg/BMSCs group. The mice were administered with PBS or BMSCs via intracerebroventricular injection. Spatial learning and memory abilities of the mice were evaluated by Morris water maze test on the 3rd day after surgery. Real-time PCR was applied to detect the mRNA expression of CX3C chemokine ligand 1 (CX3CL1), CX3C chemokine receptor 1 (CX3CR1), IL-1β, TNF-α, Nurr1, YM1, insulin-degrading enzyme (IDE) and matrix metalloproteinase 9 (MMP9). The protein levels of CX3CL1 and Aβ42 were measured by ELISA. Western blot was used to detect the protein expression of postsynaptic density protein 95 (PSD95) and synaptophysin (SYP). RESULTS: The transplanted BMSCs were observed near the hippocampus of APP/PS1 mice on the 10th postoperative day. The escape latency of the mice in Tg/PBS group was significantly longer than that in the WT/PBS mice (P<0.05). Compared with Tg/PBS group, the escape latency of Tg/BMSCs group was significantly shorter (P<0.05), and the mRNA and protein levels of CX3CL1 in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.01). The results of immunohistofluorescence staining showed that BMSC transplantation promoted the activation of microglia in the brain of WT and Tg mice. The mRNA expression of YM1 was up-regulated in WT/BMSCs group and Tg/BMSCs group (P<0.05). Compared with WT/PBS mice, the mRNA expression of TNF-α in the cortex and hippocampus of Tg/PBS group was significantly increased (P<0.05), and the mRNA expression of Nurr1 in the cortex was significantly decreased (P<0.01). Meanwhile, the mRNA expression of TNF-α in the cortex of Tg/BMSCs mice was decreased (P<0.01) and the mRNA expression of CX3CR1 and Nurr1 was up-regulated compared with Tg/PBS group (P<0.05). The results of Western blot showed that the protein levels of PSD95, p85, p110 and p-Akt in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.05). Finally, BMSC transplantation reduced the protein level of Aβ42 in APP/PS1 mice (P<0.05), and increased the mRNA expression of IDE and MMP9 in the hippocampus (P<0.05). CONCLUSION: BMSC transplantation modulates neuroinflammatory responses and promotes neuroprotective factor and synaptic protein expression, thus improving the learning and memory abilities in the APP/PS1 mice, which may be achieved by up-regulating the expression of CX3CL1.  相似文献   

Effects of soil organic matter on pH-dependent phosphate sorption by soils     

Syuntaro Hiradate  Natsuyo Uchida 《Soil Science and Plant Nutrition》2013,59(5):665-675

Effects of soil organic matter (80M) on P sorption of soils still remain to be clarified because contradictory results have been reported in the literature. In the present study, pH-dependent P sorption on an allophanic Andisol and an alluvial soil was compared with that on hydrogen peroxide (H₂0₂)-treated, acid-oxalate (OX)-treated, and dithionite-citrate- bicarbonate (DCB)-treated soils. Removal of 80M increased or decreased P sorption depending on the equilibrium pH values and soil types. In the H₂O₂ OX-, and DCB-treated soils, P sorption was pH-dependent, but this trend was not conspicuous in the untreated soils. It is likely that 80M affects P sorption of soils through three factors, competitive sorption, inhibition of polymerization and crystallization of metals such as AI and Fe, and flexible structure of metal-80M complexes. As a result, the number of available sites for P sorption would remain relatively constant in the wide range of equilibrium pH values in the presence of 80M. The P sorption characteristics were analyzed at constant equilibrium pH values (4.0 to 7.0) using the Langmuir equation as a local isotherm. The maximum number of available sites for P sorption (Q _max) was pH-dependent in the H₂0₂-, OX-, and DCBtreated soils, while this trend was not conspicuous in the untreated soils. Affinity constants related to binding strength (K) were less affected by the equilibrium pH values, soil types, and soil treatments, and were almost constant (log K ≈ 4.5). These findings support the hypothesis that 80M plays a role in keeping the number of available sites for P sorption relatively constant but does not affect the P sorption affinity. By estimating the Q _max and K values as a function of equilibrium pH values, pH-dependent P sorption was well simulated with four or two adjustable parameters. This empirical model could be useful and convenient for a rough estimation of the pH-dependent P sorption of soils.  相似文献