Application of vincristine-loaded platelet therapy in three dogs with refractory immune-mediated thrombocytopenia     

Hyung-Jin Park  Ja-Won Kim  Kun-Ho Song  Kyoung-Won Seo 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):127-130

Three dogs presented with refractory immune-mediated thrombocytopenia (IMT). All patients failed to respond to prednisone, which is considered a mainstay of immunosuppressive therapy. Vincristine-loaded platelets (VLPs), which act selectively on mononuclear phagocytes,were introduced. After the VLPs were transfused, two dogs responded quickly withimproved clinical signs while the third dogwith recurrent IMT was euthanized due to its deteriorating condition. This case report describesthe efficacy of VLP therapy in refractory IMT patients.  相似文献   

Effects of High Dietary Levels of Fresh or Oxidised Fish Oil on Performance and Blood Parameters in Female Mink (Mustela vison) During the Winter,Reproduction, Lactation and Early Growth Periods     

Birthe M. Damgaard  Christian F. B?rsting  Ricarda M. Engberg  S?ren K. Jensen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3):136-146

The effects of high dietary levels of fresh or moderately oxidised fish oil on performance and blood parameters in mink females were investigated during the winter, reproduction, and lactation periods. Furthermore, the effects of the diets on kit performance were investigated during the lactation and early growth periods. The investigation was carried out with a total of 292 females distributed in five experimental groups fed fresh fish oil stored frozen, fresh fish oil ensiled, oxidised fish oil stored frozen, oxidised fish oil ensiled, and soya oil, respectively. The females were weighed three times during the winter period, and the females and the kits were weighed at parturition and 2, 4, and 7 weeks post partum. Blood samples were collected from the females and the kits 6 and 8 weeks post partum, respectively. The results show that high dietary levels of fresh or moderately oxidised fish oil could be used for mink females during the winter and reproduction periods without any negative effects on performance, health and reproduction results. However, high levels of fish oil resulted in lower kit weights at weaning. These negative effects on kit growth were related to the dietary composition fed during the lactation and early growth periods and not to the diet used during the preceding winter and pregnancy periods. A high intake of polyunsaturated fatty acids resulted in a decreased number of blood platelets for both mink females and their kits.  相似文献   

Bioactive Lipids of Marine Microalga Chlorococcum sp. SABC 012504 with Anti-Inflammatory and Anti-Thrombotic Activities     

Katie Shiels  Alexandros Tsoupras  Ronan Lordan  Constantina Nasopoulou  Ioannis Zabetakis  Patrick Murray  Sushanta Kumar Saha 《Marine drugs》2021,19(1)

Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga Chlorococcum sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC₅₀) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of Chlorococcum sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga.  相似文献   

Marine Benthic Diatoms Contain Compounds Able to Induce Leukemia Cell Death and Modulate Blood Platelet Activity     

Siv Kristin Prestegard  Linn Oftedal  Rosie Theresa Coyne  Gyrid Nygaard  Kaja Helvik Skj?rven  Gjert Knutsen  Stein Ove D?skeland  Lars Herfindal 《Marine drugs》2009,7(4):605-623

In spite of the high abundance and species diversity of diatoms, only a few bioactive compounds from them have been described. The present study reveals a high number of mammalian cell death inducing substances in biofilm-associated diatoms sampled from the intertidal zone. Extracts from the genera Melosira, Amphora, Phaeodactylum and Nitzschia were all found to induce leukemia cell death, with either classical apoptotic or autophagic features. Several extracts also contained inhibitors of thrombin-induced blood platelet activation. Some of this activity was caused by a high content of adenosine in the diatoms, ranging from 0.07 to 0.31 μg/mg dry weight. However, most of the bioactivity was adenosine deaminase-resistant. An adenosine deaminase-resistant active fraction from one of the extracts was partially purified and shown to induce apoptosis with a distinct phenotype. The results show that benthic diatoms typically found in the intertidal zone may represent a richer source of interesting bioactive compounds than hitherto recognized.  相似文献   

The influence of oral simvastatin and vitamine-E on serum LDL oxidation and platelet activation in coronary artery disease patients     

YANG Zhi-ming  GAO Yu-zhen  XIAO Chuan-shi  BIAN Yun-fei  LIANG Shu-fen  WANG Feng-zi 《园艺学报》2000,16(6):495-497

AIM: Using simvastatin and vitamine E (Vit-E) treatment to coronary artery disease (CAD) patients with low HDL, to investigate the relationship between Ox-LDL, platelet activation and HDL. METHODS: 40 CAD patients with low HDL were divided into two groups (A and B): A group oral simvastatin, B group oral simvastatin and Vit-E. The level of serum Ox-LDL, TXB₂ and GMP-140 were measured before and after treatment. The relationship between Ox-LDL, TXB₂, GMP-140 and HDL were analysed. RESULTS:The level of serum HDL was significantly increased in A and B group after treatment and attained normal level. The level of serum Ox-LDL, TXB₂ and GMP-140 were decreased significantly after simvastatin and Vit-E treatment and neared normal. CONCLUSIONS:This study confirmed that HDL can effectively refrain LDL oxidation. It also revealed that Vit-E and simvastatin treatment were more effectively refrained platelet activation by increasement of HDL and decreasement of Ox-LDL.  相似文献   

Protective effects of WEB 2086 (PAF antagonist) and ketoprofen (NSAID) on PAF-induced changes in the morphological ultrastructure of blood platelets in calves     

Bastos da Silva  M.  Dessy  C.  Coghe  J.  David  J.-L.  Lekeux  P. 《Veterinary research communications》1998,22(4):273-291

The ultrastructure of bovine platelets was examined by transmission electron microscopy without any pretreatment (control), and after WEB 2086 (a triazolodiazepine) or ketoprofen (NSAID) pretreatment, followed by PAF infusion. The blood platelet count was also investigated. The group of calves that received WEB 2086 pretreatment before platelet-activating factor (PAF) infusion did not show a decreased number of platelets. However, in the other group, with ketoprofen pretreatment before PAF infusion, there was a rapid decrease from 1 to 3 min, while from 5 min the number of platelets recovered to the normal value. Electron microscopy revealed that pretreatment with WEB 2086 followed by PAF infusion did not alter the morphological ultrastructure of bovine platelets, except that the microtubules were briefly modified from 1 until 3 min after PAF challenge. After ketoprofen pretreatment, bovine platelets kept their regular shape, the number of dense bodies was not significantly altered, the number of mitochondria was maintained from 5 min after PAF infusion, giant platelets were not observed and the Golgi apparatus was rarely visible. Thus pretreatment with WEB 2086 and ketoprofen before PAF infusion had a protective activity on the ultrastructure of bovine platelets and, in cattle, pretreatment with WEB 2086 and ketoprofen before PAF challenge prevented the thrombocytopenia induced by PAF.  相似文献   

Effects of hydrocortisone and aminophylline on the aggregation of equine platelets in vitro     

Stefania Casella  Elisabetta Giudice  Claudia Giannetto  Simona Marafioti  Giuseppe Piccione 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(3):215-219

The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided into three different aliquots. In the first aliquot, platelet aggregation was measured after platelet activation with 1 µM and 0.5 µM ADP (Group A). In the other two aliquots, the effect of a 10 min preincubation with hydrocortisone (Group B) or aminophylline (Group C) on ADP-induced aggregation at final ADP concentrations of 1 µM and 0.5 µM was observed. Platelet aggregation, recorded by an aggregometer, was evaluated by measuring the maximum degree of platelet aggregation and the initial velocities of platelet aggregation were obtained. Our results demonstrated the inhibitory effect of hydrocortisone and the induction effect of aminophylline on equine platelet responses in vitro.  相似文献   

Effect of compound Salvia militorrhiza injection on LPS-induced disseminated intravascular coagulation in rabbits     

LIN Xi  QI Jie-zhen  QIU Peng-xin  CHEN Jia-shu 《园艺学报》2011,27(3):464-468

AIM: To evaluate the effect of compound Salvia militorrhiza(SM) injection on lipopolysaccharide (LPS)-induced experimental disseminated intravascular coagulation (DIC) in rabbits. METHODS: The LPS-induced DIC model in rabbits was performed by continuous infusion of LPS at 100 μg·kg^-1·h^-1 for 6 h. Treatment with compound SM injection or heparin was begun simultaneously with LPS infusion through the contralateral marginal ear vein.Activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were determined. The plasma levels of alanine aminotransferase (ALT) and blood urine nitrogen (BUN) were detected. The activity of protein C and antithrombi III (ATIII) was also measured.RESULTS: Continuous infusion of LPS induced gradual impairment of hemostatic parameters and damages in renal and liver functions. The intravenous administration of compound SM injection attenuated the increase in plasma levels of APTT and PT, and the decrease in plasma levels of fibrinogen and platelet induced by LPS infusion. Compound SM injection decreased the levels of ALT and BUN, reduced the damages in the liver and kidney, and also significantly inhibited the decreased plasma levels of protein C and ATIII in LPS- treated animals.CONCLUSION: Compound SM injection has a protective effect against DIC in rabbits.  相似文献   

Establishment and assessment of a method for acid elution of platelet HLA class I antigens     

LIU Xiang-fu  ZOU Yong  LIU Wen-da  LU Ying  QIN Xue-ling  LI Fang  LIAO Si-hong  YUAN Qing  LIU Gui-lian  LIN Zhe-sheng  LI Jie-fang  LIN Dong-jun 《园艺学报》2011,27(10):1995-1999

AIM: To establish an effective method for acid elution of platelet HLA class I antigens, and to evaluate the optimal condition and the feasibility of clinical application of the acid-elution technique. METHODS: Platelets were treated with citric acid buffer at different pH levels (pH=2, 3, 5, 7). Expression of HLA class I antigens and P-selectin (CD62P) on the platelet surface was analyzed by multicolor flow cytometry. The proportion of early apoptotic platelets was detected by Annexin V staining. The maximum platelet aggregation rate was determined by electrical impedance aggregometry.RESULTS: With the decrease in the pH levels of the citric acid buffer (from pH=7 to pH=2), the expression of HLA class I antigens on the platelet surface was remarkably decreased. However, the rates of platelets activation (CD62P expression) and early apoptosis (Annexin V expression) were both significantly increased. Compared with PBS, treatment of the platelets with citric acid buffer at pH 3.0 remarkably reduced the expression of platelet HLA-class I antigens (P<0.05). Although the rates of the platelet activation and apoptosis were also significantly increased (P<0.05), the aggregation of platelet was not remarkably reduced (P>0.05).CONCLUSION: Acid elution of platelet HLA-class I antigens with citric acid buffer at pH 3.0 at 0 ℃ can be use as an attempt to produce HLA-eluted platelets. This technique of acid-elution needs further improvement and standardization before clinical use.  相似文献   

毛冬青和复方丹参注射液对猪血小板细胞骨架蛋白的影响   总被引：2，自引：0，他引：2  

黄才  梁念慈 《湛江医学院学报》1993,(Z1)

本文用Triton沉淀法研究了毛冬青和复方丹参注射液对猪血小板细胞骨架蛋白的影响。结果表明:(1)毛冬青注射液对凝血酶诱导的肌动蛋白聚合和肌球蛋白与肌动蛋白微丝的结合都有强烈的抑制作用,IC_(50)分别为200μg/ml,120μg/ml;(2)复方丹参注射液(100mg/ml,含丹参生药量)可抑制凝血酶诱导的肌动蛋白聚合,抑制率为51.2％;并可完全消除凝血酶刺激的肌球蛋白与微丝的结合。本文从新的角度阐明了毛冬青和复方丹参注射液抗血小板作用的机制。  相似文献