Establishment and assessment of a method for acid elution of platelet HLA class I antigens     

LIU Xiang-fu  ZOU Yong  LIU Wen-da  LU Ying  QIN Xue-ling  LI Fang  LIAO Si-hong  YUAN Qing  LIU Gui-lian  LIN Zhe-sheng  LI Jie-fang  LIN Dong-jun 《园艺学报》2011,27(10):1995-1999

AIM: To establish an effective method for acid elution of platelet HLA class I antigens, and to evaluate the optimal condition and the feasibility of clinical application of the acid-elution technique. METHODS: Platelets were treated with citric acid buffer at different pH levels (pH=2, 3, 5, 7). Expression of HLA class I antigens and P-selectin (CD62P) on the platelet surface was analyzed by multicolor flow cytometry. The proportion of early apoptotic platelets was detected by Annexin V staining. The maximum platelet aggregation rate was determined by electrical impedance aggregometry.RESULTS: With the decrease in the pH levels of the citric acid buffer (from pH=7 to pH=2), the expression of HLA class I antigens on the platelet surface was remarkably decreased. However, the rates of platelets activation (CD62P expression) and early apoptosis (Annexin V expression) were both significantly increased. Compared with PBS, treatment of the platelets with citric acid buffer at pH 3.0 remarkably reduced the expression of platelet HLA-class I antigens (P<0.05). Although the rates of the platelet activation and apoptosis were also significantly increased (P<0.05), the aggregation of platelet was not remarkably reduced (P>0.05).CONCLUSION: Acid elution of platelet HLA-class I antigens with citric acid buffer at pH 3.0 at 0 ℃ can be use as an attempt to produce HLA-eluted platelets. This technique of acid-elution needs further improvement and standardization before clinical use.  相似文献   

毛冬青和复方丹参注射液对猪血小板细胞骨架蛋白的影响   总被引：2，自引：0，他引：2  

黄才  梁念慈 《湛江医学院学报》1993,(Z1)

本文用Triton沉淀法研究了毛冬青和复方丹参注射液对猪血小板细胞骨架蛋白的影响。结果表明:(1)毛冬青注射液对凝血酶诱导的肌动蛋白聚合和肌球蛋白与肌动蛋白微丝的结合都有强烈的抑制作用,IC_(50)分别为200μg/ml,120μg/ml;(2)复方丹参注射液(100mg/ml,含丹参生药量)可抑制凝血酶诱导的肌动蛋白聚合,抑制率为51.2％;并可完全消除凝血酶刺激的肌球蛋白与微丝的结合。本文从新的角度阐明了毛冬青和复方丹参注射液抗血小板作用的机制。  相似文献   

Presumptive immune‐mediated thrombocytopenia secondary to massive Africanized bee envenomation in a dog     

Renee K. Fenty DVM  DACVECC  Domenico Bianco DVM  PhD  DACVIM 《Journal of Veterinary Emergency and Critical Care》2013,23(6):652-656

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Effect of safflower injection on LPS-induced disseminated intravascular coagulation in rabbits     

ZHU Wei-wei  LI Meng-jia  MA Lian-shun  LIU Mei  LIN Xi  ZHU Lin-yan  XIAO fei 《园艺学报》2013,29(8):1481-1486

AIM：To study the effect of safflower injection on lipopolysaccharide (LPS)-induced disseminated intravascular coagulation (DIC) in rabbits. METHODS：The rabbit model of DIC was induced by continuous infusion of LPS at a dose of 100 μg·kg-1·h-1 for 6 h. The treatment with safflower injection and heparin were started simultaneously with LPS infusion through the contralateral marginal ear vein. The activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrin(ogen) concentration were measured. The plasma levels of fibrin(ogen) degradation products (FDP), alanine aminotransferase (ALT) and blood urine nitrogen (BUN) were detected. The activity of protein C and antithrombin III (AT III) and the concentration of tumor necrosis factor α (TNF-α) were also determined. RESULTS：The gradual injury of hemostatic parameters was induced by continuous infusion of LPS. APTT, PT, BUN, ALT and plasma TNF-α were increased obviously. The platelet count, fibrinogen, FDP, protein C and AT III were decreased significantly. The intravenous administration of safflower injection attenuated the increases in the levels of APTT, PT, BUN, ALT, FDP and plasma TNF-α induced by LPS infusion, and improved the plasma levels of fibrinogen, platelet, protein C and AT III. CONCLUSION: Safflower injection protects against LPS-induced DIC in rabbits．  相似文献   

Thrombocytosis: a retrospective study of 165 dogs     

Neel JA  Snyder L  Grindem CB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(2):216-222

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Complement, polymorphonuclear leukocytes and platelets in acute experimental respiratory reactions to Aspergillus     

S A Olenchock  M S Mentnech  J C Mull  M E Gladish  F H Green  P C Manor 《Comparative immunology, microbiology and infectious diseases》1979,2(1):113-124

An experimental complement-dependent model of an acute allergic response to inhalation of Aspergillus spores has been used to examine the roles of polymorphonuclear leukocytes (PMN) and platelets in mediating this reaction as measured by decreases in arterial oxygen tensions (Pa_O2). Normal rabbits selectively depleted of PMN by nitrogen mustard (HN₂) showed a decrease in Pa_O2 later than that observed in untreated animals. The HN₂-treated group also showed a marked decrease in platelets after challenge, not seen in the similarly challenged untreated group or in unchallenged HN₂-treated animals. These data demonstrate the importance of PMN in complement-mediated host responses to aerosols of fungal spores. In the absence of the PMN, platelets appear capable of initiating a similar, albeit delayed, reaction, thus further emphasizing the flexibility of pulmonary defense mechanisms against inhaled particles.  相似文献   

Estimation of platelet counts on feline blood smears     

Séverine T  Cripps PJ  Mackin AJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(2):42-45

Blood samples were obtained from 50 cats admitted for hematologic evaluation at the Royal (Dick) School of Veterinary Studies. Manual platelet counts were done using a hemacytometer, and the average number of platelets per oil immersion field (1,000X magnification) was determined on stained blood smears. A hemacytometer count was not obtained for one sample because of a failure in erythrocyte lysing. In nine samples, obvious platelet clumps in the blood smear prevented accurate determination of the number of platelets per oil immersion field. Hemacytometer counts on these nine samples ranged from 260-587 X 10 (3) platelets/microliter, suggesting that platelet clumps on a blood smear were usually associated with adequate platelet numbers. Simple regression analysis of hemacytometer counts and the average umber of platelets per oil immersion field for the remaining 40 samples yielded correlation coefficients (r) of 0.776 on untransformed data, and 0.892 on log10-transformed data. Each platelet per oil immersion field represented a circulating platelet count of approximately 20 X 10(3)/microliter, similar to conversion factors reported for dogs and human beings. It was concluded that estimation of platelet number on stained blood smears is a simple and quick method that appears to be reliable over a wide range of platelet counts in cats.  相似文献   

Polysaccharide extracted from laminaria japonica aresch inhibits platelet activation and protects endothelial cells     

XIE Lu  LI Jing  LIU Ai-qun 《园艺学报》2007,23(4):674-677

AIM: To study the relationship between the effects of polysaccharide L01 extracted from laminaria japonica aresch on platelet activation and endothelial cells. METHODS: A rat model of endothelial injury was established via injecting adrenaline. The percentage of platelet adhesion was evaluated by filtration method, the activation of platelet aggregation was observed on a glass plate with collodion membrane, the content of vWF in rat plasma was measured by ELISA, the damaged degree of aortic vascular endothelial was evaluated by immunity histochemistry. RESULTS: The percentage of platelet adhesion and aggregation in model group were higher than those in NS group from the 3th and 4th day during the model made (P<0.05, P<0.01). The percentage in both L01 high-dose group (50 mg/kg) and low-dose group (10 mg/kg) at the 4th and 5th day was lower than that in model group (P<0.05, P<0.01). The content of vWF in rat plasma in model group was higher than that in NS group and in L01 high-dose group at 4th day (P<0.05). The same results were presented by the comparison among model group and NS group, both L01 high-dose group and low-dose group at the 5th (P<0.05). The measure of intact endodermis lengths (μm) stained by immunohistochemistry demonstrated that the length in model group was shorter than that in NS group (P<0.05), whereas the length in L01 high-dose group and low-dose group was obviously longer than that in model group (P<0.05) at the 4th and 5th day. CONCLUSION: The inhibitory effect of L01 on platelet activation may be related with its protective effect on vascular endothelial cells.  相似文献   

Platelet function in clinically healthy cats and cats with hypertrophic cardiomyopathy: analysis using the Platelet Function Analyzer‐100     

Karl E. Jandrey  Jeffrey W. Norris  Kristin A. MacDonald  Mark D. Kittleson  Fern Tablin 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(4):385-388

Background: There is currently no simple analytical tool for the evaluation of hypercoagulability in cats. The Platelet Function Analyzer‐100^® (PFA‐100; Dade Behring Inc., Deerfield, IL, USA) is a bench‐top machine that evaluates platelet function by measuring closure time (CT) in citrated whole blood under high shear conditions. We hypothesized that cats with hypertrophic cardiomyopathy (HCM) have up‐regulated platelet function, which shortens their CT and increases their risk for thromboembolic events. Objectives: The goals of this study were to: (1) establish a feline reference interval for CT using the PFA‐100, (2) measure CT in blood from cats with HCM, and (3) determine if there is a measurable difference between the CT of healthy cats compared with cats with HCM. Methods: Citrated blood samples from 42 clinically healthy cats and 30 cats with HCM were analyzed according to manufacturer's specifications. CT was measured in triplicate and the mean value was used for analysis. Transformed data were compared between clinically healthy cats and cats with HCM using a Student's t‐test, and among cats with mild, moderate, or severe HCM using ANOVA. Results: The median CT of clinically healthy cats was 64 seconds (range 43–176 seconds). The median CT of cats with HCM was 74 seconds (range 48–197 seconds). There was no significant difference in CT between cats with HCM and clinically healthy cats. There also were no significant differences in cats with mild, moderate, or severe HCM. Conclusions: A feline reference interval for PFA‐100 CT will be useful in future studies of platelet function in cats. Cats with HCM do not have shorter CTs when compared with clinically healthy cats.  相似文献   

A novel point mutation in the β1‐tubulin gene in asymptomatic macrothrombocytopenic Norfolk and Cairn Terriers     

Maria Elena Gelain  Walter Bertazzolo  Giuseppina Tutino  Elena Pogliani  Francesco Cian  Mary K. Boudreaux 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(3):317-321

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