Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat   总被引：1，自引：0，他引：1  

ZHANG Long-yu  ZHANG Gai-sheng  ZHAO Xin-liang  YANG Shu-ling 《中国农业科学(英文版)》2013,(6):941-950

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.  相似文献   

不同复合菌剂对甘薯营养成分的影响     

李昊坤  韩燕红  刘全兰  王家林 《现代农业科技》2023,(22)

本文以朝日8号 为材料进行田间小区试验，研究了9组不同复合菌剂对甘薯产量和品质的影响。与对照相比，苏云金芽孢杆菌Bt1、肠杆菌属Es和戊糖片球菌PP1形成的组合1的增产效果最高，与对照相比增产17.02%；复合菌剂处理均显著改变了甘薯的营养品质和DPPH自由基清除率，增加或减少了水分、灰分、粗蛋白、粗纤维、总糖、还原性糖、胡萝卜素、维生素C和多酚等营养成分的含量，增加或减少了DPPH自由基清除率。复合菌剂组合1较好的改善了甘薯。  相似文献   

洗涤效果与温度关系的理论探讨     

安秀林  王一芳  刘海平 《河北北方学院学报(自然科学版)》2000,(3)

表面活性剂是洗涤剂的必要组分。分析温度与不同表面活性剂的溶解度、表面张力和加溶作用的关系,结果表明,温度对洗涤效果的影响是多方面的。  相似文献   

聚乙烯醇共混改性膜作为农药种衣剂成膜剂的性能研究   总被引：5，自引：0，他引：5       下载免费PDF全文

潘立刚  周一万  叶海洋  郝双红  张兴 《农药学学报》2005,7(2):160-164

以水溶性聚合物聚乙烯醇(PVA)和水不溶性聚合物乙酸乙烯-乙烯共聚物(VAE)乳液为原料,采用溶液共混法制备了PVA-VAE共混膜,为农药成膜剂的制备开辟了新途径。红外光谱、透射电镜、激光粒度仪测试结果显示,PVA溶液中的胶束被打开,体系的比表面积急剧增大,两相间的界面消失,说明共混体系分子间具有良好的相容性;透水性与耐水性测定结果表明,PVA-VAE的质量比为80∶ 20、70∶ 30、60∶ 40时其共混膜的性能适用于农药种衣剂的成膜剂;种子发芽试验显示,与单独使用PVA膜相比,引入适当比例VAE后的共混膜用于种子包衣,其耐水性显著提高,渗水率适宜,对种子发芽无明显影响。  相似文献   

对悬浮离心法回收昆虫病原线虫方法的改进   总被引：3，自引：0，他引：3  

简恒  杨怀文 《中国生物防治》1995,11(1):13-16

本文对Ｓａｎｕｄｅｒｓ的悬浮离心回收线虫法进行了改进研究。保留第１次离心后上清液中含有的线虫，结果使小卷蛾线虫的平均回收率由３２．２％上升到６０．２％；离心处理对线虫的存活率及其对害虫的侵染率没有显著影响，但鞘的保持率有所降低。用浓度为２００ｇ／Ｌ盐液替代了浓度为４５４ｇ／Ｌ的糖液，对线虫回收率无影响，但成本可以降低。  相似文献   

氮肥对保水剂吸水保肥性能的影响   总被引：7，自引：0，他引：7  

苟春林  杜建军  曲东  崔英德  毋永龙 《干旱地区农业研究》2006,24(6):78-84

测定保水剂在不同氮肥(尿素、碳酸氢铵、硝酸铵、硫酸铵、氯化铵)溶液中的吸水倍率和对肥料养分的吸持量,分析不同氮肥对保水剂吸水、保肥性能的影响。结果表明:保水剂在各种氮肥溶液中吸水倍率显著下降,并随肥料浓度的增加下降幅度增加;受影响程度聚丙烯酸钠盐型保水剂大于聚丙烯酰胺-丙烯酸盐型保水剂;不同氮肥对保水剂吸水倍率的影响按尿素、碳酸氢铵、硝酸铵、硫酸铵、氯化铵顺序递增。保水剂在大量吸水的同时,也对溶液中的肥料分子或者离子有吸持作用,吸持量均随肥料浓度的增加而增加;保水剂和肥料品种不同,吸持量也不同;除尿素外,保水剂对肥料的吸持率随肥料浓度的增加而降低,最高浓度下,吸持率按硝酸铵、碳酸氢铵、氯化铵、硫酸铵、尿素次序依次增大。  相似文献   

营养添加剂对枯草芽孢杆菌G3 str在土壤中繁殖及其抑菌物质分泌能力的影响     

程洪斌  陈伟  顾真荣  陈红漫 《中国生物防治》2006,22(2):142-145

从生防菌G3菌株筛选到的抗链霉素自然突变株G3^str,保持G3原有的抑菌活性,产伊枯草菌素和生物表面活性素,能抑制多种植物病原真菌.25℃下,营养添加剂对G3^str菌在不灭菌土壤中前5d有一定的增殖作用;10d后G3^str菌在土壤中主要以芽孢的形态存活;60d后,G3^str菌在土壤中仍以106cfu/g水平存活.G3^str菌在土壤中增殖时分泌少量的伊枯草菌素,但被快速分解;同时分泌较多的生物表面活性素,营养添加剂的加入明显促进其分泌.  相似文献   

Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicide     

Thaís C.C. Fernandes 《Pesticide biochemistry and physiology》2007,88(3):252-259

The trifluralin is an agent that promotes a cellular damage due to its direct action on the microtubules. This action leads to a decontrol in the cellular division, bringing about polyploid cells. In this work, we show the evidences that the exceeding genetical material of theses polyploidizated cells tends to be eliminated from the nucleus in the form of micronucleus. Our analyses prove this fact, both by the presence of a number of cells carrying micronucleus, and by the evidences of the elimination of the exceeding material itself, after exposition of the Allium cepa root tips tested with several concentration of trifluralin herbicide. It was noticed that the residual concentration induced a number of polyploid cells, micronuclei and mini cells. Inferences about the implications of the elimination of genetic material from micronuclei, such as cell viability and apoptosis, are also presented.  相似文献   

Biocontrol efficacy of Colletotrichum truncatum for hemp sesbania (Sesbania exaltata) is enhanced with unrefined corn oil and surfactant     

C. DOUGLAS BOYETTE  ROBERT E. HOAGLAND  MARK A. WEAVER 《Weed Biology and Management》2007,7(1):70-76

In greenhouse and field experiments, an oil-in-water emulsion of unrefined corn oil and Silwet L-77 increased the biological weed control efficacy of Colletotrichum truncatum (Schw.) Andrus et Moore for control of the weed, hemp sesbania ( Sesbania exaltata [Raf.] Rydb. ex A.W. Hill). The surfactant – corn oil emulsion stimulated germination and appressoria formation in vivo and in vitro and delayed the need for dew. We hypothesize that the corn oil protected the conidia from desiccation during the dew-free period and the surfactant stimulated spore germination and appressoria formation. In field experiments conducted over 3 years, a single application of a 50% (v/v) unrefined corn oil tank mixture containing 0.2% (v/v) Silwet L-77 surfactant controlled hemp sesbania in soybeans an average of 95%. Aqueous fungal suspensions or adjuvants alone did not visually affect or control hemp sesbania. The soybean yields were significantly higher in the plots where weeds were effectively controlled. These results suggest that formulating C. truncatum in unrefined corn oil and surfactant greatly increases its infectivity and the biocontrol potential of this pathogen.  相似文献   

甲壳素等生物制剂对仙客来枯萎病的防治效果及生长促进作用研究     

黄建明  郑志兴  尉文彬  武玉环  曹丽霞 《中国植保导刊》2007,27(10):12-13

对比试验结果表明,甲壳素单独使用、甲壳素与放线菌混合使用、甲壳素与放线菌和枯草杆菌混合使用灌根,对仙客来枯萎病均具有明显的防治效果,仙客来成活率显著提高,其防效与常用化学农药相当。同时对仙客来的生长发育均有促进作用。处理后根的数量、根系的鲜重和干重均有显著增加,并使叶片数增多,冠径增大。此外,单独使用甲壳素还使土壤中放线菌数量明显增加。  相似文献