S-genotype identification based on allele-specific PCR in Japanese pear     

Kenji Nashima  Shingo Terakami  Sogo Nishio  Miyuki Kunihisa  Chikako Nishitani  Toshihiro Saito  Toshiya Yamamoto 《Breeding Science》2015,65(3):208-215

Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S¹–S⁹ and S^k) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S¹/S¹–S⁹/S⁹ and S^4sm/S^4sm) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs.  相似文献   

果树自交不亲和机理及应用研究进展   总被引：6，自引：0，他引：6  

陈超  李保国  齐国辉  钮力亚  苏智海 《河北林果研究》2005,20(1):86-90

综述了果树自交不亲和性机理的一些研究进展。主要包括自交不亲和性的表现、自交不亲和基因(S-等位基因)的表达与克隆、由S基因编码所决定的植物自交不亲和性的关键功能性蛋白(S-糖蛋白)的鉴定、S-糖蛋白的结构与功能、果树S基因型确定等以及应用自交不亲和机理的几种方法，并提出了该领域尚待解决的问题。  相似文献   

中国梨品种自交不亲和新基因的分离鉴定   总被引：4，自引：0，他引：4  

谭晓风  乌云塔娜  中西テッ  李秀根  曹玉芬  杨谷良 《中南林业科技大学学报(自然科学版)》2005,25(1):1-3

采用分子生物学技术、田间杂交试验和生物信息学方法研究了中国梨自交不亲和基因和品种的S基因型.从中国白梨和沙梨品种中分离鉴定了15个梨自交不亲和新基因;分析了新S基因的序列特征;确定了中国白梨中至少存在17个S等位基因,中国沙梨中至少存在10个S等位基因;鉴别了34个梨品种的S基因型.这些新S基因的发现和品种S基因型的确定为中国梨品种的优化配置、丰产栽培和遗传改良提供了科学依据.  相似文献   

Identification of Self-Incompatibility Genotypes in Some Sand Pears （Pyrus pyrifolia Nakai） by PCR-RFLP Analysis   总被引：1，自引：0，他引：1  

GU Qing-qing  ZHANG Qing-lin  HU Hong-jü  CHEN Qi-liang  LUO Zheng-rong 《中国农业科学(英文版)》2009,8(2):154-160

The identification of self-incompatibility genotype （S-genotype） will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-RNase sequencing. The Sgenotypes of these cultivars were determined as follows： Huali 1 S1S3, Shounan S1S3, Xizilti S1S4, Qingxiang S3S7, Sanhua S2S7, Huangmi （Imamuranatsu） S1S6, Huali 2 S3S4, Baozhuli S7S33, Cangxixueli S5S15. S-RNase alleles （S1 to S9） in sand pear could be identified effectively by PCR-RFLP analysis.  相似文献   

Identification of self-incompatibility alleles (S) by PCR-RFLP in radish (Raphanus sativus L.)   总被引：1，自引：0，他引：1  

S. Niikura  S. Matsuura 《Euphytica》1998,102(3):379-384

From 16 inbred lines of cultivated radishes (Raphanus sativus L.), 6 S-alleles tentatively named S²⁰¹ to S²⁰⁶ were identified, and their dominance relationships were examined. Among the S-alleles, S²⁰¹, S²⁰², S²⁰³ and S²⁰⁴ were found to be co-dominant. These 4 S-alleles showed dominance with S²⁰⁵ in pollen and with S²⁰⁶ in both pollen and stigma, while S²⁰⁵ and S²⁰⁶ were co-dominant. Polymerase chain reaction (PCR) was performed using the radish inbred lines randomly selected from the 6 S-allele groups. The primers were based on the highly conserved sequences of the S-locus specific glycoprotein (SLG) genes in Brassica oleracea. As a result of the PCR, a single DNA fragment of about 1.16kb was amplified as expected from the original sequence of B.oleracea. The S-allele specific pattern in the restriction fragments of the PCR products (PCR-RFLP) was confirmed for the first group of S-alleles (S²⁰¹, S²⁰², S²⁰³ and S²⁰⁴). However, for the second group of the S-alleles (S²⁰⁵ and S²⁰⁶), no PCR products were obtained. The usefulness of the PCR-RFLP in a radish breeding program is described. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Assessing inbreeding in perennial ryegrass (Lolium perenne) as a step towards F1 hybrid breeding     

Caitlin Harris  Madison Hall  Ruby Arrowfield  Rowan Herridge  Colin Eady  Richard Macknight  Lynette Brownfield 《Plant Breeding》2023,142(4):518-526

Perennial ryegrass is an important turf and forage grass in temperate regions worldwide. Limited genetic gains have been made with current breeding strategies compared with other grass crops, such as rice and maize, which benefit from Filial 1 (F1) hybrid breeding. One of the largest constraints on hybrid breeding in ryegrass is self-incompatibility preventing inbreeding, as homozygous parental lines are required to develop hybrids with maximal hybrid vigour. Obligate outcrossing in ryegrass has resulted in cultivars with high levels of heterozygosity, lacking trait uniformity across the population. A naturally occurring self-fertile (SF) locus that overcomes the self-incompatibility system has been identified in a European perennial ryegrass population. This study crossed the SF locus into an elite cultivar, producing a self-compatible population that was inbred for several generations. Genotyping-by-sequencing (GBS) was used to assess the population structure and degree of inbreeding in the self-compatible population. Phenotypic analysis indicated that increased homozygosity did not necessarily affect growth and performance. This study concludes that self-compatible ryegrass is a promising tool for hybrid breeding and agronomic improvement of perennial ryegrass.  相似文献   

S-genotype characterisation of 10 Kernel-apricots native to China     

Mengpei Liu  Pei Hou  Lihua Zhang  Yu Dong 《The Journal of Horticultural Science and Biotechnology》2019,94(2):237-242

The reproductive mechanism of Kernel-apricot involves Gametophytic self-incompatibility (GSI) which is under S-allele control. It encodes a polymorphic pistil ribonuclease (S-RNase), which is multiallelic and ideal markers for S-genotypes characterisation. In this study, six S-RNase primers were used to identify theS-genotypes of 10 Kernel-apricot, including three self-design primers and three universal primers. Ten S-alleles were identified in 10 Kernel-apricot through sequencing and comparing in NCBI. Moreover, some Kernel-apricot S-genotypes were confirmed by experiment of fruit-setting ratio and pollen tube growth. In conclusion, S-genotypes information of Kernel-apricot will be helpful for breeders and growers to plan compatible hybridised combination for ensuring effective pollination and high fruit-setting ratio.  相似文献   

白菜新品种‘秋冠’     

李桂花  陈汉才  张艳  黎庭耀 《园艺学报》2017,44(Z2):2673-2674

 ‘秋冠’是利用自交不亲和系js11-2-1与自交系js39-23-1配制而成的中早熟优质白菜新品种。株高22.1 cm,开展度27.5 cm,单株质量294 g。叶柄长6.6 cm,宽5.1 cm,叶柄厚11.0 mm。可溶性固形物含量为4.0%,蛋白质为2.08%,还原糖为0.796%,维生素C 5.36 mg · kg^-1,粗纤维0.40%。春季产量27 930 kg · hm^-2,秋季产量34 185 kg · hm^-2。田间表现适应性强,抗逆性较强。  相似文献   

甘蓝型油菜自交不亲和系1010及其在杂种优势育种中的利用研究     

安彩泰  李学才 《甘肃农业大学学报》1990,25(1):51-56

本文报告了甘蓝型油莱(Brassica napus L.)自交不亲和系1010的选育经过和方法,分析和评价了该系的配合力、自交不亲和性的保持和繁殖体系等问题。  相似文献   

甘蓝自交不亲和细胞信号转导的功能基因研究进展     

牛义  王志敏  汤青林  宋明  朱利泉  王小佳 《西南农业学报》2006,19(5):956-961

自交不亲和性是开花植物防止近亲繁殖而广泛存在的一种遗传屏障,在育种工作中意义重大,它在遗传上受一个带有复等位基因的多态性S基因座所控制。本文综述了近年来在甘蓝自交不亲和信号转导途径中相关功能基因的研究进展。SRK基因和SP11/SCR基因是控制甘蓝自交不亲和性的两个关键因子。SRK基因是雌蕊柱头中自交不亲和反应的专一决定因子,编码SP11/SCR基因的蛋白在花药壁和花粉中特异表达。编码SLG基因的蛋白在自交不亲和反应中起增强SRK活性的作用。另外也对信号传导途径中下游蛋白质的作用模式作了展望。  相似文献