不同类型小肽产品对断奶仔猪生产性能及血液生化指标的影响     

孙占田  马秋刚  刘志敏 《中国饲料》2007,(20):15-16

试验研究了3种不同类型的小肽产品等蛋白取代喷雾干燥血浆蛋白粉对21d断奶仔猪的生产性能和血液生化指标的影响。选用体重近似的192头(21±1)d断奶的皮杜长大四元杂交仔猪,随机分为4个处理,每个处理6个重复,每个重复栏8头猪。4个处理日粮分别为:添加2%喷雾干燥血浆蛋白粉的对照组和分别添加2%小肽产品A、B和C等蛋白取代血浆蛋白粉的T1、T2和T3试验组。试验期为21d。试验结果表明,添加3种不同肽产品等蛋白取代血浆蛋白粉对断奶仔猪的生产性能和血液生化指标均无显著影响(P>0.05),小肽产品可以部分等蛋白取代断奶仔猪日粮中的血浆蛋白粉。  相似文献   

低能饲粮添加植酸酶对仔猪养分利用率、血清理化指标及生长性能的影响   总被引：7，自引：1，他引：7  

陈文  陈代文 《中国畜牧杂志》2006,42(17):24-27

选用24头长白×荣昌杂交仔猪进行2×2因子的代谢试验,试验设2个有效磷水平(0.36%和0.26%)、2个植酸酶添加水平(0、750FTU/kg),共4个处理组,每个处理组设6个重复仔猪起始均重16.9kg,饲粮DE12.8MJ/kg,试验期2周。结果表明:饲粮添加植酸酶,仔猪平均日增重(ADG)、增重/kgW0.75(GPW)、F/G、增重/代谢能(GPM)、蛋白质物质价值(BV)、净蛋白效率比(PER)、钙消化率(CD)、钙沉积率(CR)、磷消化率(PD)和磷沉积率(PR)分别提高10.6%、7.2%、7.9%、7.7%、11.6%、6.7%、4.3%、4.9%、19.6%(P<0.01)、21.3%(P<0.01),每千克增重的饲粮成本(CPG)降低6.6%;植酸酶对仔猪血清理化指标无显著影响,但血清磷浓度趋于升高,血清钙、血清尿素氮浓度趋于降低。降低饲粮有效磷,ADG、GPW、F/G、GPM、BV、PER、PD和PR分别降低3.8%、3.3%、3.2%、4.6%、9.6%、3.2%、7.7%、6.9%,CD、CR和CPG分别增加11.1%、11.6%、3.0%(P>0.05)。  相似文献   

金钱豹血液理化值的测定   总被引：2，自引：1，他引：2  

王兴金  李少基  李婉萍  谢高基  陈武  黄勉  陈足金  陈绚姣 《野生动物》2006,27(2):33-35

广州动物园科研中心于2003年10月至2005年3月间共采集11只金钱豹的34份血液样本，对40项临床常用的血液生理生化值进行了测定，结果用SPSS11．0统计软件进行统计分析。  相似文献   

Human serum albumin interaction with paraquat studied using spectroscopic methods     

Gencheng Zhang  Yanqing Wang  Hongmei Zhang  Shuhe Tang  Weihua Tao 《Pesticide biochemistry and physiology》2007,87(1):23-29

The interaction between herbicide paraquat and human serum albumin (HSA) was investigated by fluorescence and UV/Vis absorption spectroscopy. Paraquat can strongly quench the intrinsic fluorescence of HSA by static quenching and nonradiative energy transferring; The hydrophobic and electrostatic interactions play a major role in stabilizing the complex. The binding site number n and apparent binding constant K_A, corresponding thermodynamic parameters ΔG, ΔH, ΔS at different temperatures, were calculated. The distance r between donor (HSA) and acceptor (paraquat) was obtained according to fluorescence resonance energy transfer. The effect of paraquat on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy.  相似文献   

Binding of phenthoate to bovine serum albumin and reduced inhibition on acetylcholinesterase     

Chao Xu  Anping Zhang 《Pesticide biochemistry and physiology》2007,88(2):176-180

Organophosphorus pesticides (OPs) are of environmental significance due to their high toxicity to animals. Binding to plasma proteins may effective influence the toxicological properties of xenobiotics. In an attempt to evaluate the affinity of phenthoate (PTA) to bovine serum albumin (BSA) and inhibitory ability of bound PTA to acetylcholinesterase (AChE), we investigated the interactions between phenthoate (PTA) and bovine serum albumin (BSA) using tryptophan fluorescence quenching and subsequent inhibition on AChE activity by PTA. The results showed that PTA caused the fluorescence quenching of BSA because of the formation of a PTA-BSA complex. Quenching constants (K_sv), determined using the Sterns-Volmer equation to provide a measure of the binding affinity between PTA and BSA at 303, 306, 310 and 313 K were (3.4295 ± 0.0763) × 10⁻⁴, (3.2446 ± 0.0635) × 10⁻⁴, (3.0434 ± 0.0856) × 10⁻⁴ and (2.8262 ± 0.0569) × 10⁻⁴ M⁻¹, respectively. The thermodynamic parameters, ΔH and ΔS were −25.04 kJ mol⁻¹ and 168.94 J mol⁻¹ K⁻¹, respectively, which indicated that the electrostatic interactions played a major role in PTA-BSA association. The presence of BSA consistently reduced the inhibitory ability of PTA on AChE, with the relative activity being increased from 46.98 to 61.71% for the concentration range of BSA between 0 and 4.0 g L⁻¹.  相似文献   

Alanine aminotransferase apoenzyme in dogs     

Mesher CI  Rej R  Stokol T 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1998,27(1):26-30

Unusually low serum activity of alanine aminotransferase (ALT) was detected in a Rottweiler dog with gastric dilatation-volvulus. Activity of ALT in the same sample was found to be much higher (estimated increase of 14,225%) when measured by methods adding the cofactor pyridoxal-5'-phosphate (P5P), indicating that nearly all serum ALT was in the apoenzyme form. An investigation was undertaken to determine the frequency of high serum aminotransferase apoenzyme levels in dogs. Eighty canine serum samples submitted to the Clinical Pathology Laboratory at Cornell University were assayed for ALT and aspartate aminotransferase (AST) in the presence or absence of exogenous P5P. In 79 dogs, inclusion of P5P in the LAT assay resulted in a median decrease in AST activity of -6.3% (range -33.3% to 25.0%) in all 80 dogs. One dog had an increase of 336% in ALT activity after inclusion of P5P in the assay, but lacked a similar increase in AST activity. The reason for the high levels of ALT apoenzyme in the 2 dogs was not determined.  相似文献   

Normal haematopoiesis,cellular components and stainable iron content in the bone marrow of camels (Camelus dromedarius)     

Nazifi  S.  Tadjalli  M.  Bedeltavana  A.R. 《Veterinary research communications》1998,22(1):11-20

Bone marrow samples were collected from the ribs of 20 healthy adult male Iranian camels (Camelus dromedarius). The bone marrow smears were stained using Wright's stain. Blood samples were collected at the same time for routine haematological examination. The development and morphology of the blood cells in the bone marrow of the camels were similar to those of other domestic species. The mean myeloid/erythroid ratio was 1.21, the mean erythroid percentage was 42.7% and the mean myeloid percentage was 52.0%. The mean percentage of other cells was 5.3%. The mean percentage of eosinophils in the myeloid series was higher than in other domestic species. Iron stores, estimated from Perl's stain, ranged from scant (1+) to moderate (3+) but most samples had 2+ iron content. All peripheral blood results were within reference ranges.  相似文献   

中草药饲料添加剂对河西肉牛血液生化指标影响的研究     

周学辉  杨世柱  李伟  郭兆斌 《中国草食动物》2012,(6):23-25

为了提升河西肉牛产品特别是血液制品的质量及产业效益,利用饲料中添加中草药添加剂的方法对河西肉牛架子牛进行了育肥、屠宰试验。采取低剂量中草药组、高剂量中草药组和对照组肉牛血样,用专门仪器和方法测定了血液中5种血清矿物质离子、7种血清代谢产物及5种血清酶等生化指标的含量。结果表明:高剂量中草药组肉牛血清中的Na、Mg离子及乳酸脱氢酶含量均极显著高于低剂量组和对照组(P<0.01),Cl离子含量极显著高于对照组(P<0.01),其葡萄糖、肌酐、甘油三酯含量均显著高于对照组(P<0.05),谷丙转氨酶显著低于其他两组肉牛(P<0.05),而谷草转氨酶(AST)却显著高于其他两组肉牛(P<0.05);低剂量组和高剂量组肉牛血清中的高密度脂蛋白极显著高于对照组(P<0.01),碱性磷酸酶极显著低于对照组肉牛(P<0.01),而谷丙/谷酸则显著高于对照组肉牛(P<0.05)。结论:试验配制的中草药饲料添加剂对肉牛血液生化指标有良好的影响,对高剂量组肉牛的影响更为明显;饲喂中草药饲料添加剂的肉牛生长发育、营养状况均显著好于对照组,但高剂量组更优;利用高剂量中草药饲料添加剂饲喂的肉牛,其血液在制造保健用品方面有广阔开发前景。  相似文献   

抓捕应激对鸡血清酶活性的影响研究   总被引：1，自引：0，他引：1  

邱伟海 《畜禽业》2007,(9):2-4

鸡受到抓捕应激后,开始血液中SOD活性降低,到第三天明显低于对照组;随着抓捕应激次数的增多,SOD活性又迅速升高,第十二天显著高于对照组,第九天、第十五天分别极显著高于对照组。CPK活性都高于对照组,而且随着应激次数的增加,CPK活性有逐渐上升的趋势。但抓捕应激对血清中GOT、GPT、APK、LDH、GSH-Px等酶活性的影响不显著。  相似文献   

枯草芽孢杆菌替代抗生素对平养和笼养肉鸡的影响研究          下载免费PDF全文

张红兵  李晓珍  苏子峰 《家畜生态学报》2012,33(6):50-54

 将240只1日龄罗斯308（ROSS 308）肉仔鸡，按照饲养方式（地面平养和笼养）以及添加枯草芽孢杆菌替代抗生素与否（替代和不替代）分为4个处理组（1组：抗生素地面平养组；2组：抗生素笼养组；3组：枯草芽孢杆菌地面平养组；4组：枯草芽孢杆菌笼养组），进行42 d的饲养试验。结果显示：试验全期，肉鸡末体重和饲料/增重比指标，4组显著优于（P<0.05）1组，2组和3组之间无差异（P>0.05）。21日龄和42日龄肉鸡血清生化指标分析显示：4组肉鸡血清白蛋白浓度显著大于1组；且血清尿酸浓度4组小于1组；而2组和3组之间所有血清测定指标均无差异。以上结果证明，无论是地面平养还是笼养方式，枯草芽孢杆菌替代抗生素对改善肉鸡生长性能均有良好效果，其作用机理主要是提高饲料蛋白的营养利用率.  相似文献