Population dynamics of Aspergillus flavus in the air of an intensively cultivated region of south-west Arizona     

C. H. Bock ‡  B. Mackey  P. J. Cotty † 《Plant pathology》2004,53(4):422-433

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香菇新菌株Cr-72的杂交选育及推广应用     

蔡衍山 《中国食用菌》2004,23(2):12-15

本文报道了香菇Cr - 72杂交优良菌株的选育过程和推广应用的结果。同时 ,也讨论了香菇品种间单孢杂交育种若干技术。  相似文献   

应用血球计数板检测灵芝破壁孢子粉破壁率的研究   总被引：9，自引：0，他引：9  

尚晓冬  李明容  王南  谭琦 《食用菌学报》2005,12(2):37-40

灵芝孢子粉称样量与孢子计数(血球计数板法)的回归分析结果表明,两者显著相关。在此基础上建立了灵芝破壁孢子粉破壁率的检测方法。破壁率48.9%的标准样品,日内相对标准偏差(RSD,n=14)为10.34%,回收率(mean±SD,n=14)为94.9%±9.81%;破壁率68.8%、80.0%和90.6%的标准样品,日内相对标准偏差(RSD,n=10)分别为4.14%、3.43%和2.22%,回收率分别为101.2%±4.2%、98.76%±3.39%和99.73%±2.21%(mean±SD,n=10)。  相似文献   

真姬菇风干子实体菌种分离与无性孢子形态特征   总被引：4，自引：0，他引：4  

韩绍英  姜彦祥 《中国食用菌》1998,17(5):36-37

从真姬菇风干子实体上分离到菌种,在普通培养基上（如ＰＤＡ）均能生长,优良菌株用于生产。在菌丝体上大量产生无性孢子,本文报道了分生孢子和体眠孢子的形态特征。  相似文献   

Field testing of honeybee-dispersed Trichoderma spp. to manage sunflower head rot (Sclerotinia sclerotiorum)     

A. R. Escande †  F. S. Laich  M. V. Pedraza 《Plant pathology》2002,51(3):346-351

Efficacy of Trichoderma spp. to reduce sunflower head rot caused by Sclerotinia sclerotiorum was evaluated in the field. A mixture of six isolates, including Trichoderma koningii , T. aureoviride and T. longibrachiatum , was tested in five field trials at Balcarce, Argentina. Trichoderma formulation (TF) included Trichoderma conidia and viable hyphal fragments, industrial talc and milled corn kernels. Honeybees ( Apis mellifera ) were used to disperse TF for six weeks from the onset of flowering. Two days after the first TF delivery, sunflower heads were inoculated with S. sclerotiorum ascospores. When 100 g TF was taken by honeybees in a 10-h per day period, head rot incidence was significantly reduced. This approach was successful in reducing disease incidence until physiological maturity of the crop, in environments highly conducive to head-rot development.  相似文献   

Quantitative relationships between inoculum of Melampsora larici-epitea and corresponding disease on Salix   总被引：1，自引：0，他引：1  

M. H. Pei†  C. Ruiz  T. Hunter  G. M. Arnold  C. Bayon 《Plant pathology》2002,51(4):443-453

Six Salix clones were inoculated with urediniospores of four isolates of Melampsora larici-epitea at five inoculum levels using a leaf-disc method. Disease reactions were recorded using a digital camera; the number and size of uredinia were examined using image analysis software; and spore yield per leaf disc was measured. In three Salix / Melampsora combinations, S.  ×  mollissima 'Q83'/Q1 (LET4); S. viminalis '78183'/V1 (LET1); and S.  ×  stipularis /V1, pustule numbers increased as inoculum density became higher. In the remainder, S. viminalis 'Mullatin'/V1; S.  ×  calodendron /DB (LET3); and S. burjatica 'Korso'/K (LR1), pustule numbers initially increased, then decreased as inoculum densities exceeded 140–360 spores per disc. Calculated infection efficiency ranged from 0·11 to 0·20 on the three willows inoculated with V1: 0·16–0·68 for S.  ×  calodendron /DB; 0·20–0·55 for 'Q83'/Q1; and 0·07–0·48 for Korso/K. In single-spore inoculations, up to 10% of spores produced single uredinia. Infection efficiency increased sharply between inoculum densities of 1–40 spores per leaf disc. Spore yield was more closely correlated to pustule area (accounting for 61·2% variance for the combined data) than to the number of pustules (42·7% variance). For spore yields in relation to pustule numbers, clone-specific individual lines having different intercepts and slopes fitted significantly better than either a single line for all the tested willows, or parallel lines fitted to each clone ( P  < 0·001). For spore yields in relation to pustule area, clone-specific individual parallel lines were significantly better than a single line ( P  < 0·001).  相似文献   

十种藓类植物孢子萌发与原丝体发育的初步研究   总被引：23，自引：0，他引：23  

赵建成  李秀芹  张慧中 《干旱区研究》2002,19(1):32-38

对10种藓类植物（红蒴立碗藓、无疣墙藓、钟帽藓、短尖叶墙藓、中华细枝藓、柳叶藓、多褶青藓、狭叶绢藓、东亚绢藓）的成熟孢子在光照培养箱中进行人工培养，培养液为Knodson C培养液，对照组为自来水。在光学显微镜下描绘和照相记录了这10种藓类孢子萌发与原丝体发育的基本过程，并对孢子的萌发能力和时间，原丝体系统发育的类型、特点进行了分析和讨论。  相似文献   

酪酸梭状芽孢杆菌培养条件的研究   总被引：6，自引：0，他引：6  

匡群  孙梅  施大林 《饲料工业》2005,26(10):36-39

为使酪酸梭状芽孢杆菌的菌体浓度和芽孢转化率都达到最大,我们对其培养条件进行了研究。结果表明:葡萄糖1.0%、胰蛋白胨1.0%、酵母膏0.5%、牛肉浸膏0.3%,适量合适的无机盐类,接种前活化菌种,接种量10%,培养36h可获最大菌体浓度和芽孢转化率。  相似文献   

In Vitro Antifungal Activity of Dihydrochelerythrine and Proteomic Analysis in Ustilaginoidea virens     

WEI Qinghui  CUI Daizong  ZHENG Baojiang  ZHAO Min 《水稻科学》2023,30(3):257-273

Dihydrochelerythrine(DHCHE) is an isoquinoline compound, which has distinct antifungal activity and can induce apoptosis. The antifungal activity of DHCHE against five rice pathogenic fungi was studied in vitro. At the concentration of 7.5 mg/L, DHCHE exhibited the highest efficacy among tested compounds in inhibiting mycelium growth, with an inhibition rate of 68.8% in Ustilaginoidea virens, which was approximately 2.4 times of that of validamycin(28.7%). After exposure to DHCHE, transmission e...  相似文献   

国内葡萄白粉病菌对戊唑醇的抗药性研究     

高琪  刘梅  谭海芸  王琦  李兴红  张玮 《农学学报》2023,13(6):49-54

为明确国内葡萄白粉病菌(Erysiphe necator)对戊唑醇的抗药性,采用实时荧光定量PCR,基于对葡萄白粉病菌CYP51基因第495位点突变A495T的检测,分析国内5个省份分离获得的134株菌株对戊唑醇的抗药性,利用孢子萌发法测定供试菌株对戊唑醇的敏感性,分析2种方法结果的相关性。结果表明,实时荧光定量PCR方法检测到国内葡萄白粉病菌对戊唑醇已出现抗药性,抗性频率为35.07%,不同地区存在差异。孢子萌发法结果显示,供试菌株对戊唑醇的EC₅₀范围为0.085～280.917μg/mL,均值为24.208μg/mL,不同地区菌株对戊唑醇的敏感性差异较大,EC₅₀最大值和最小值之比为2.5～3304。孢子萌发法与实时荧光定量PCR方法间的检测结果趋势一致并显著相关。  相似文献