Expression of TMEM16A as a calcium-activated chloride channel in Fischer rat thyroid follicular epithelial cells and its electrophysiologic pro-perties     

HAO Feng  BAI Xue-song  JU Xiao-hong  FANG Fang  ZANG Yu-xuan  ZHU Hang-fei  XIANG Guo-yan  ZHANG Yun-qiao  YUAN Zhong-hai 《园艺学报》2014,30(9):1633-1639

AIM：To investigate the expression of transmembrane protein 16A(TMEM16A) in Fischer rat thyroid follicular epithelial (FRT) cells and its electrophysiologic properties. METHODS：The eukaryotic expression vector of pUB6/V5-TMEM16A was constructed and transfected into FRT cells by liposome-mediated transfection. In order to obtain the high efficiency of gene transfection and expression, the quantity and ratio of lipid/DNA complexes were optimized. The FRT cells stably expressing TMEM16A were gained by the selection with blasticidin and confirmed by the techniques of RT-PCR and immunofluorescence. The expression and location of TMEM16A in the FRT cells were observed under an inverted fluorescence microscope. TMEM16A protein was associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent protein and patch-clamp technique. RESULTS：The results of double digestion and sequencing indicated that TMEM16A was cloned into pUB6/V5. The results of RT-PCR and immunofluorescence confirmed that TMEM16A was expressed in the FRT cells after transfection with TMEM16A. The classical calcium-activated chloride channel currents were recorded in the FRT cells stably expressing TMEM16A by the technique of patch-clamp and halide-sensitive fluorescent protein YFP-H148Q/I152L. CONCLUSION：The protein expression of TMEM16A in the FRT cells was observed. TMEM16A is the molecular identity of calcium-activated chloride channels.  相似文献   

外源性硫化氢对骨骼肌疲劳的影响简     

孙洪兆  赵术珍  鲁凯  侯丰田  韩佳秀  赵鹏飞  张兴梅  张丽  张雪  韩增晓  刘晴晴  刘雪联 《中国畜牧兽医》2014,41(1):240-242

试验旨在探讨硫化氢供体硫氢化钠改善疲劳状态下肌力减退的作用。采用脉冲式电流直接刺激腓肠肌作为疲劳模型,对比硫氢化钠和任氏液分别对蟾蜍腓肠肌疲劳的影响。结果表明,硫氢化钠在浓度为5×10^-4、1×10^-3、2×10^-3 mol/L时,离体腓肠肌达到最大收缩幅度90%、50%和10%的时间极显著长于在任氏液作用下的离体腓肠肌(P<0.01);5.6 mg/kg硫氢化钠也极显著延长了在体腓肠肌达到最大收缩幅度90%、50%和10%的时间(P <0.01)。表明外源性硫化氢具有延缓肌肉疲劳的作用。  相似文献   

The evaluation of secondary stress response of grass carp (Ctenopharyngodon idella, Val. 1844) after exposing to the saline water     

H. Yavuzcan-Yıldız  M. Kırkavgaç-Uzbilek 《Fish physiology and biochemistry》2001,25(4):287-290

The stress response of grass carp to salinity was evaluated assessing the levels of hematocrit, leucocrit, plasma glucose, sodium, potassium and calcium. Mean hematocrit values increased following exposure to salinity. Mean leucocrit values elevated after exposure to salinity for 24 h, then decreased. Mean plasma glucose increased during saline exposure for 48 h and then decreased to normal values. Plasma sodium and chloride levels were increased to their highest values after 48 h. While plasma potassium value increased with the exposure time, plasma calcium values were not affected by salinity exposure.  相似文献   

Induction of larval settlement and metamorphosis of the abalone, Haliotis discus hannai larvae using bromomethane and potassium chloride     

K. H. Kang  B. H. Kim  J. M. Kim 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):249-259

Larvae of Haliotis discus hannai were induced to settle and metamorphose by exposure to seawater supplemented with bromomethane. The settlement and metamorphosis of veliger larvae were observed with bromomethane concentrations of 300, 600, 900, 1200, 1500 ppm on the benthic diatom plates. The highest settlement and metamorphosis rates of larvae were observed in bromomethane concentrations of 600 ppm.

Abalone larvae were induced to settle and metamorphose by exposure to seawater supplemented with potassium chloride. The settlement and metamorphosis of veliger larvae was observed in potassium chloride concentrations of 14, 19, 24 and 29 mM on the benthic diatom plates. The highest settlement and metamorphosis rates of larvae were observed in potassium chloride concentrations of 14–19 mM.  相似文献   

Extent of chum salmon egg softening caused by sodium hypochlorite as an antifungal agent     

Chutima Khomvilai  Masaaki Kashiwagi  Chanin Sangrungruang  Motoi Yoshioka 《Fisheries Science》2007,73(5):1205-1207

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Minimising aerobic respiratory demands could form the basis to sub-lethal copper tolerance by rainbow trout gill epithelial cells in vitro     

Richard W. Smith  Maria Jönsson  Dominic F. Houlihan  Peter Pärt 《Fish physiology and biochemistry》2001,24(2):157-169

Mechanisms of Cu tolerance were investigated in respiratory epithelial cell cultures, from rainbow trout gills, by studying O₂ consumption and protein synthesis rates, intracellular Na concentration and TER. The lowest concentration found to reduce O₂ consumption was 25 M Cu. This did not affect either protein synthesis rate or intracellular Na concentration and was interpreted in terms of copper tolerance; i.e., how these two energetically demanding processes are maintained despite a reduction in aerobic ATP supply. The relationship between protein synthesis rate and synthesis cost is exponential and the cost of protein synthesis in gill cells was found to be minimal (i.e., this cell occupies a position on the asymptotic section of the protein synthesis rate/synthesis cost model) and unaffected by 25 M Cu. Thus protein synthesis rates could be maintained since any reduction would represent an insignificant energy saving. Intracellular Na concentrations and O₂ consumption rates were linearly correlated suggesting reducing intracellular maintenance costs would have a greater significance in terms of overall energetic conservation. Intracellular Na maintenance costs, calculated from O₂ consumption rates and intracellular Na concentrations, were found to decline after exposure to 25 M Cu. Since TER was unaffected this implied the reduced costs arose from membrane `channel arrest'. Thus the Na/K ATPase energy demands, associated with maintaining intracellular Na concentration, could be reduced by decoupling metabolic demand and membrane function. Therefore this study may demonstrate how the flexibility of cellular energetics enables gill epithelial cells to tolerate sub-lethal Cu.  相似文献   

Improved survival of rohu, Labeo rohita (Hamilton-Buchanan) and silver carp, Hypophthalmichthys molitrix (Valenciennes) fingerlings using low-dose quinaldine and benzocaine during transport     

Mahmud Hasan  & Amrit N Bart 《Aquaculture Research》2007,38(1):50-58

The effects of fingerlings immersion in low‐dose benzocaine (15 and 30 mg L⁻¹, silver carp and rohu) and quinaldine (100 μL L⁻¹ silver crap and 250 μL L⁻¹ rohu) for 1, 3 and 6 h on stress responses and survival of rohu, Labeo rohita and silver carp, Hypophthalmichthys molitrix fingerlings were evaluated in a transport simulation experiment. Both quinaldine and benzocaine showed low mortalities (0–2%). The total mortality in control (with no anaesthesia) was 30% for rohu and 14% for silver carp. Quinaldine and benzocaine‐treated fingerlings had significantly higher plasma chloride levels than the control in both species. Benzocaine, quinaldine, as well as the control, had an initial elevation of plasma cortisol levels. Benzocaine lost its effectiveness after 3 h exposure while quinaldine persisted throughout the 6 h experimental period. Both sedatives reduced bacterial build‐up compared with the control. No post‐exposure mortality was observed for any of the transport methods assessed 48 h after the treatment. This study suggests that the use of low‐dose benzocaine or quinaldine during transport has positive effect on the survival and health of rohu and silver carp fingerlings.  相似文献   

Action of Clathrodin and Analogues on Voltage-Gated Sodium Channels     

Steve Peigneur  Ale? ?ula  Nace Zidar  Fiona Chan-Porter  Robert Kirby  David Madge  Janez Ila?  Danijel Kikelj  Jan Tytgat 《Marine drugs》2014,12(4):2132-2143

Clathrodin is a marine alkaloid and believed to be a modulator of voltage-gated sodium (Na_V ) channels. Since there is an urgent need for small molecule Na_V channel ligands as novel therapeutics, clathrodin could represent an interesting lead compound. Therefore, clathrodin was reinvestigated for its potency and Na_V channel subtype selectivity. Clathrodin and its synthetic analogues were subjected to screening on a broad range of Na_V channel isoforms, both in voltage clamp and patch clamp conditions. Even though clathrodin was not found to exert any activity, some analogues were capable of modulating the Na_V channels, hereby validating the pyrrole-2-aminoimidazole alkaloid structure as a core structure for future small molecule-based Na_V channel modulators.  相似文献   

复硝酚钠对低温下黄瓜种子萌发和幼苗耐寒性的影响     

黄斌  李文科  孙敏涛  闫妍  王君  贺超兴  于贤昌  李衍素 《核农学报》2022,36(4):845-855

为缓解低温胁迫对黄瓜(Cucumis sativus L.)幼苗的伤害,以黄瓜中农106为试验材料,研究不同浓度复硝酚钠(CSN)浸种对低温胁迫下黄瓜种子萌发及萌发后生长的影响,以及CSN灌根处理对黄瓜幼苗壮苗效果及幼苗耐寒性的影响。结果表明,适宜浓度的CSN浸种能显著提高低温胁迫下黄瓜种子的发芽率、发芽势、发芽指数、活力指数、侧根数和鲜重,以50 mg·L^-1浓度效果最佳。在黄瓜育苗过程中,100 mg·L^-1的CSN灌根处理能显著提高穴盘苗质量和壮苗指数,低温胁迫下CSN通过促进矿质元素吸收,提高幼苗叶绿素含量、净光合速率、根系活力、可溶性蛋白含量和抗氧化酶活性,提高生长素(IAA)和油菜素内酯(BR)含量,降低活性氧和丙二醛(MDA)含量以提高幼苗的耐寒性。本研究为培育黄瓜壮苗和提高黄瓜幼苗耐寒性提供了新方法,也为复硝酚钠在设施蔬菜上的应用提供了技术支持。  相似文献   

氮肥与复硝酚钠复配对南方大豆光合特性和产量及品质的影响     

郝青南  杨芳  汪嫒嫒  龙泽福  杨中路  陈海峰  单志慧  邓军波  周新安 《中国油料作物学报》2022,44(3):610

为确定南方大豆增产增效的栽培管理措施及光合特性与产量品质的相关性,本研究以5个南方主栽大豆品种为材料,于初花期（R1）和鼓粒期（R5）进行叶面喷施不同浓度的尿素和复硝酚钠,试验设置5个处理,3个种植区域,每个种植区3次重复,通过2年的数据,研究了叶面喷施氮肥以及氮肥与复硝酚钠复配对南方大豆品种产量、品质以及光合特性的影响。结果表明,增施氮肥可以提高南方大豆各品种的叶绿素SPAD值、净光合速率等光合特性,可提高单株有效荚数、单株粒重等产量构成因素进而提高大豆的产量;同时,氮肥与复硝酚钠复配与单独施用氮肥相比可使油6019、中豆44、中豆41和中豆63的产量增加,对脂肪和蛋白含量影响较小,对高蛋白品种皖豆28未起到增产的效果,但可使脂肪含量有所增加;经过氮素处理后,油6019的产量与品质呈现正相关性。综上所述,氮肥与复硝酚钠复配喷施具有进一步协同提高南方大豆品种产量和品质潜力,但由于对不同品种作用效果存在差异,针对不同基因型大豆品种需要制定最佳的处理方案。  相似文献