转基因玉米转化体特异性PCR检测技术研究   总被引：1，自引：3，他引：1  

武海斌  路兴波  孙红炜  杨崇良  李天玉  李宝笃 《玉米科学》2009,17(1):65-70

根据不同转基因玉米中重组DNA结构,分别对转基因玉米Bt11、Bt176、Mon810、Mon863、TC1507、GA21和NK603设计转化体特异性引物进行PCR检测。在此基础上分别以玉米内参照基因(ZSSIIb)作对照,对Bt11、Mon810、TC1507和GA21、NK603、Bt176分别作两对四重PCR反应,实现在同一个PCR反应管中同时对3种转基因玉米及内参照基因的特异性检测。  相似文献   

甜高粱育种思路探讨   总被引：6，自引：4，他引：2  

马涌  张福耀  赵威军  郑娟 《山西农业科学》2011,(6):619-621

甜高粱生物产量高,含糖量高,乙醇转化率高,抗逆性强,是能源作物,对其合理开发利用,对我国粮食安全、能源安全具有重大意义。我国甜高粱育种水平落后,现有品种不能适应生产发展的需要。创新育种思路,可从选育粮糖兼用型品种和自交不结实的品种方面进行甜高粱品种选育。  相似文献   

88%异丙甲草胺乳油防除高粱田杂草田间药效评价     

马长山  王胜华  张浩然  石继岭 《黑龙江农业科学》2014,(7):70-72

为寻找高粱田适宜除草剂,2013年在林甸县农业科学技术推广中心试验园区试验地进行了高粱播后苗前土壤封闭处理化学除草试验。结果表明:在高粱播后苗前土壤封闭喷施88%异丙甲草胺乳油1 350~1 650g·hm-2,对一年生禾本科杂草稗草、狗尾草和部分阔叶杂草藜、反枝苋等具有较好的防除效果,控草时间长达40d以上,对高粱生长安全。  相似文献   

水稻籼粳杂种一代生育期的表现   总被引：3，自引：1，他引：3  

董世钧  李春寿 《中国水稻科学》1995,9(2):77-81

 采用生育期不同的籼型野败恢复系和保持系与典型粳稻和籼粳中间型亲本配制成80份籼粳杂种一代组合，用亚种肉品种间杂交为对照，研究了籼粳稻杂种一代生育期的表现。结果表明，以籼粳亚种间杂种一代生育期优势最强，一般都超过双亲平均值；品种间杂种一代生育期优势最弱，接近中亲值；籼或使型亲本配籼粳中间型亲本，杂种一代生育期优势介于上述两类型组合之间。籼粳杂交由于双亲的不同，组合间F₁生育期优势差异很大，有的组合甚至出现负向优势．这种生育期优势差异既受双亲本身生育期的迟早所支配，如杂种一代生育期长短排列顺序为中籼／晚粳＞中籼／早或中粳＞早籼或早熟中籼／晚粳＞早籼或早熟中籼／早或中粳；又受籼型亲本所含的野败恢、保遗传基因所制约，凡用籼型野败恢复系配制的籼粳杂种一代其生育期优势明显减弱，并与双亲籼、粳遗传距离的大小有关，杂种一代生育期优势随双亲籼、便遗传距离的扩大而增强。  相似文献   

水稻除草剂敏感基因导入恢复系的研究   总被引：11，自引：2，他引：11  

朱启升  王安东  杨前进 《杂交水稻》2000,15(3):5-6

以农林８号ｍ为主体亲本与优良恢复系杂交,成功地将除草剂（苯达松）敏感基因转育到恢复系中,携带该基因的恢复系不但对苯达松敏感（抽穗期用１８００￣２７００ｍｌ／ｈｍ＾２苯达松喷施可使整穗或整株死亡）,而且所配组合具有较强的杂种优势。  相似文献   

百日草自交系花粉萌发条件及花粉活力研究   总被引：1，自引：0，他引：1  

叶要妹  张佳祺  张双凤  包满珠 《华中农业大学学报》2007,26(5):693-696

研究了不同培养基、采集时间、贮藏温度和湿度等因素对百日草花粉离体培养萌发效果的影响。结果表明:花粉萌发较适宜的培养基为ME3 16%PEG4000 12%蔗糖;上午10:00采集花粉萌发率要显著地高于下午3:00采集的;在4℃湿润条件下贮藏18 h,J6花粉生活力下降较小,而在室温干燥条件下几乎失去活力;相对湿度在66%以下时极不利于花粉保存;11个百日草自交系花粉离体萌发率差异显著。  相似文献   

农网配电线路损耗分析     

纪长波  夏顺忠 《农业科技与装备》2021,(1)

配电网起着输送和分配电能的作用,线路损耗给供电企业造成很大的成本投入,降低能源的利用率。分析配电网线路损耗的原因,提出降低线路损耗的具体措施,以期为配电网节能降耗、安全运行提供参考。  相似文献   

60个玉米自交系的SSR标记分析   总被引：5，自引：0，他引：5  

李凌雨  孟全业  王学雄  闫彩清  王圆荣  马玉光 《华北农学报》2007,22(3):25-29

利用SSR标记研究了60个山西省主要玉米(Zea maysL.)自交系的遗传变异。用23对扩增带型稳定的引物,从供试自交系中检测出91个等位基因变异,每对引物检测出等位基因2~7个,平均为3.96个,平均多态性信息含量为0.600。UPGMA聚类分析将60个自交系划分为唐四平头,旅大红骨,PN,BSSS,Lancaster,Suwan等6个类群,化群结果与其系谱关系和育种家经验基本一致。  相似文献   

SUSTAINABLE NUTRIENT MANAGEMENT PACKAGE FOR COST-EFFECTIVE BIOENERGY BIOMASS PRODUCTION     

Sampson Agyin-Birikorang  George A. O’Connor  John E. Erickson 《Journal of plant nutrition》2013,36(12):1881-1900

Commercial fertilizer (particularly nitrogen) costs account for a substantial portion of the total production costs of cellulosic biomass and can be a major obstacle to biofuel production. In a series of greenhouse studies, we evaluated the feasibility of co-applying Gibberellins (GA) and reduced nitrogen (N) rates to produce a bioenergy crop less expensively. In a preliminary study, we determined the minimum combined application rates of GA and N required for efficient biomass (sweet sorghum, Sorghum bicolor) production. Co-application of 75 kg ha^?1 (one-half of the recommended N rate for sorghum) and a modest GA rate of 3 g ha^?1 optimized dry matter yield (DMY) and N and phosphorus (P) uptake efficiencies, resulting in a reduction of N and P leaching. Organic nutrient sources such as manures and biosolids can be substituted for commercial N fertilizers (and incidentally supply P) to further reduce the cost of nutrient supply for biomass production. Based on the results of the preliminary study, we conducted a second greenhouse study using sweet sorghum as a test bioenergy crop. We co-applied organic sources of N (manure and biosolids) at 75 and 150 kg PAN ha^?1 (representing 50 and 100% N rate respectively) with 3 g GA ha^?1. In each batch of experiment, the crop was grown for 8 wk on Immokalee fine sand of minimal native fertility. After harvest, sufficient water was applied to soil in each pot to yield ～1.5 L (～0.75 pore volume) of leachate, and analyzed for total N and soluble reactive P (SRP). The reduced (50%) N application rate, together with GA, optimized biomass production. Application of GA at 3 g ha^?1, and the organic sources of N at 50% of the recommended N rate, decreased nutrient cost of producing the bioenergy biomass by ～$375 ha^?1 (>90% of total nutrient cost), and could reduce offsite N and P losses from vulnerable soils.  相似文献   

Changes in sorghum production,soil P forms and P use efficiency following long-term application of manure,compost and straw in a Ferric Lixisol     

Dohan Mariam Soma  Delwendé Innocent Kiba  Nana Ewusi-Mensah  Zacharia Gnankambary  François Lompo  Michel Papoaba Sedogo 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2018,68(5):401-411

Low phosphorus availability in cultivated soils limits sustainable crop production in sub‐Saharan Africa. This study aimed at evaluating the effect of long‐term application of different types of organic amendments on soil P forms, P use efficiency and sorghum yields. A long term experiment established in 1980 at Saria in Burkina Faso, comparing the effects of manure, compost and sorghum straw was used. Manure and compost significantly increased organic P and resin‐P by about 35% and 64%, respectively after 10 and 32 years of sorghum cultivation, and HCl‐P after 32 years of cultivation compared to the control. Manure significantly increased NaHCO₃-Pi and NaOH-Pi by 63% and 26%, respectively compared to the control. Sorghum straw had little effect on measured soil P forms. Manure and compost were the best in increasing sorghum grain yield, which effect were strongly correlated to soil pH, carbon and nitrogen. The partial factors productivities of P resulting from the application of studied organic amendments were similar and low, but significantly higher than that of the control treatment. Organic amendments with high P content, maintaining soil carbon and pH could be used to improve soil P availability, sorghum yield and reduce the demand for mineral phosphorus fertilizers.  相似文献