Follicular fluid stimulates capacitation and acrosome reaction in alpaca sperm (Vicugna pacos)          下载免费PDF全文

Z Bravo  M Valdivia 《Reproduction in domestic animals》2018,53(3):629-635

The follicular fluid exerts an effect on the sperm capacitation of several species; however, these effects vary according to species, both in the sperm motility and in the subsequent acrosome reaction. In this study, the effect of alpaca follicular fluid (aFF) on the motility and acrosome reaction of alpaca spermatozoa was observed, using follicular fluid of three follicle sizes: small (<3 mm), medium (3‐6 mm) and large (>6 mm), in a concentration of 30%. Sperm motility at the first hour of incubation with aFF of small follicles was 48.0%, with aFF of medium follicles it was 43.33% and with aFF of large follicles, it was 34.53%, while control averaged 26.00%. At the second hour, control achieved an average of 28.13%, treatment with aFF from small follicles showed an average of 46.53%, with aFF from medium follicles it was 40.00% and with aFF from large follicles it was 35.60%. The acrosome reaction after 4 hours of incubation was 30.06% for control, whereas for aFF of small follicles it was 66.3%, with aFF of medium follicles it was 58.86% and for aFF of large follicles, it was 67.63%. In the case of sperm motility, a significant difference is demonstrated for all treatments in relation to the control at the first hour, whereas only the treatments with aFF of small and medium follicles show a significant difference with respect to the control at the second hour. In the case of the acrosome reaction, all treatments with follicular fluid show a significant difference with respect to the control. It was concluded that alpaca follicular fluid favours sperm capacitation and the acrosome reaction in alpaca spermatozoa.  相似文献   

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm          下载免费PDF全文

A Pinyopummin  S Mahasawangkul  G Nunklang  K Kornkaewrat  S Laopiem  S Koonjaenak  P Wattananit 《Reproduction in domestic animals》2018,53(2):525-533

The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris‐glucose‐egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr. In experiment 1 (n = 18), semen was diluted to 1:1, 1:3, 1:7 and 1:15 with TE (volume per volume). There were no significant changes in sperm viability and sperm with normal acrosome integrity among dilutions, but sperm motility and motility velocities were greater (p < .05) in the 1:1 dilution than those of the 1:7 and 1:15 dilutions at 48 hr of storage. In experiment 2, supplemented SP was derived from elephants and stallions. In experiment 2.1, diluted semen (1:7 dilution) was restored with SP to obtain a 1:2 proportion (n = 8). Sperm motility, viability and sperm with normal acrosome integrity were similar among treatments, but motility velocities were greater (p < .05) with stallion SP at 48 hr of storage. In experiment 2.2, diluted semen (1:15 dilution) was restored with SP to obtain a 1:3 proportion (n = 10). Sperm viability and sperm with normal acrosome integrity were similar among treatments at 48 hr of storage. However, sperm motility and motility velocities were greater (p < .05) with stallion SP than those of others. In conclusion, elephant sperm motility was affected by a dilution effect and restoration of SP proportion with stallion SP, but not with elephant SP, could improve motility in chilled highly diluted sperm.  相似文献   

哺乳动物附睾特异GPx5功能及其调节机制的研究进展     

栾兆进  张家新 《中国畜牧兽医》2018,45(9):2500-2506

精子在附睾中的成熟过程是哺乳动物雄配子获得受精能力的关键。谷胱甘肽过氧化物酶-5（glutathione peroxidase-5,GPx5）作为附睾特异性表达的抗氧化酶具有强抗氧化作用,可调节附睾微环境中的活性氧浓度,保护精子免受脂质过氧化损伤,以维持精子DNA完整性。GPx5还可能对精子活力和顶体反应产生一定影响。GPx5基因的染色体定位及其外显子数存在一定的种间差异,其在不同物种、部位和发育期的表达具有特异性,受雄激素、PEA3因子和ETV4家族等调节。作者就哺乳动物附睾特异GPx5基因的结构与定位、表达特性、功能及其调节机制的研究进展进行综述,以期为进一步研究精子抗氧化机制和提高雄性动物繁殖力提供理论依据。  相似文献   

Effects of Two Different Cooling Devices for Testicles Transport on Stallion Epididymal Sperm Quality     

《Journal of Equine Veterinary Science》2016

This study evaluates the effects of two cooling devices and temperature for testicles storage on epididymal sperm quality after 24 hours; different levels of seminal plasma (0% and 10%) were evaluated on sperm after recovering. Testicles from six stallions were recovered immediately after castration (2) or at the slaughterhouse (4); of the same animal, one testicle was placed in Equitainer (+8°C), the other in a styrofoam box with ice (+3°C). After 24 hours, the temperature of parenchyma was measured, and testicles and epididymal were weighted. Sperm were flushed from the cauda epididymides with Kenney extender, total sperm number recorded and motility and viability evaluated immediately after flushing (T0) with or without 10% SP (G1 Eq 0%, G2 Eq 10%, G3 Ice 0%, G4 Ice 10%). Motility and viability were evaluated after 24 hours and 48 hours of storage at +4°C. Temperature of the parenchyma was lower in testicles stored in ice compared to Equitainer (3.2 ± 0.6°C and 8.6 ± 2.5°C, respectively; P < .05). Motility and viability at T0 were similar (P > .05) in G1 and G3, whereas addition of SP after recovery significantly improved motility only in samples stored in Equitainer (G2). Viability was higher (P < .05) in G2 than in G4. At T24 and T48, no differences (P > .05) in sperm quality were found between storage methods or samples with or without SP. In conclusion, equine testicles can be safely stored either at lower (+3°C) or higher (+8°C) temperature than +5°C. This can be useful, especially when testicles are shipped in a hot climate, where devices cannot guarantee optimal refrigeration conditions.  相似文献   

Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze‐dried sperm          下载免费PDF全文

Nguyen Thi Men  Kazuhiro Kikuchi  Tadashi Furusawa  Thanh Quang Dang‐Nguyen  Michiko Nakai  Atsunori Fukuda  Junko Noguchi  Hiroyuki Kaneko  Nguyen Viet Linh  Bui Xuan Nguyen  Atsushi Tajima 《Animal Science Journal》2016,87(11):1325-1333

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疫苗支原体检验中液体培养基保质期试验   总被引：1，自引：1，他引：0  

张瑞婷 《中国兽药杂志》2008,42(10):29-31

介绍了病毒性活疫苗支原体检验用液体培养基冷冻保存的方法,在有效的保质期内,可提高支原体检验的速度和效率,保证检验工作的均一性和准确性.  相似文献   

不同输精部位对奶牛X性控冻精情期受胎率的影响     

梁小军  宁小波  张俊丽  王天新  张秀陶  曹忠良 《动物医学进展》2008,29(9)

为了验证不同输精部位对情期受胎率的影响,在发情开始后的20h～24h内进行人工授精,分别对子宫颈内口、子宫体分岔处、滤泡发育侧的子宫角大弯处、滤泡发育侧的子宫角小弯处4个不同输精部位的情期受胎率结果进行统计,分别为43．339／6、50．00％、77．979／6、80．00％,各组间差异板显著（P〈0．01）。试验结果表明,距离精子和卵子结合部位（输卵管壶腹部）越近,情期受胎率越高。表明最佳的输精部位是在滤泡发育侧的子宫角大弯到小弯之间。  相似文献   

两种方法保存嗜酸乳杆菌效果的比较研究   总被引：1，自引：0，他引：1  

朱志宁  赵燕  黄惠  任晓明 《动物医学进展》2008,29(11)

在相同的温度和时间条件下,比较滤纸片法和甘油生理盐水冻存法保存嗜酸乳杆菌的效果。用滤纸片法和甘油生理盐水冻存法,分别进行不同温度的嗜酸乳杆菌保存试验;并于预定时间内,对各处理组分别进行复苏及预定检测。在室温和4℃条件下,用滤纸片法保存的嗜酸乳杆菌出现了生化特性和形态的异常状况。随着保存时间的延长,滤纸片保存法比甘油生理盐水冻存法的嗜酸乳杆菌存活量明显减少。两种方法保存的菌种,均表现为-80℃组的嗜酸乳杆菌存活量明显大于-20℃组。说明在-80℃温度条件下,甘油生理盐水冻存法比滤纸片法具有较大的嗜酸乳杆菌存活量。  相似文献   

不同微囊冷冻保存HK-2细胞效果比较   总被引：1，自引：0，他引：1  

潘广林  任宏涛  顾玉兰  孙志杰 《动物医学进展》2008,29(10)

观察不同类型微囊冻存细胞的效果,为微囊冻存选择合适的冷冻保护剂。应用高压脉冲静电液滴发生器制作液化的海藻酸钠-多聚赖氨酸-海藻酸钠（APA）微囊和海藻酸钠-钙胶珠包被人肾小管上皮细胞（HK-2）;用二甲基亚砜和甘油作为冷冻保护剂冷冻未包被的HK-2细胞、APA微囊包被的HK-2细胞、胶珠包被的HK-2细胞;解冻后用MTT法测定细胞活性、考马斯亮蓝法测定蛋白质含量、Hochest33258荧光染料测定DNA含量。结果表明,解冻后液化微囊内HK-2细胞的细胞活性、蛋白质含量、DNA含量均显著高于胶珠内细胞（P〈0．05）,但与未包被组差异不显著（P〉0．05）。DMSO作为冷冻保护剂,细胞活性及细胞蛋白质含量均高于甘油组（P〈O．05）,但两者在DNA含量上无差异（P〉0．05）。液化微囊与DM～SO合用时,细胞活性及细胞蛋白质含量、DNA含量均高于液化微囊与甘油合用时（P〈0．05）。液化微囊解冻后稳定性优于胶珠。液化微囊冷冻保存效果优于胶珠,DMSO适于微囊冷冻。  相似文献   

Effect of Semen Extenders and Storage Time on Sperm Morphology of Four Chicken Breeds     

《The Journal of Applied Poultry Research》2008,17(1):101-108

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