隐性乳房炎乳汁中体细胞和病原菌及酶间的相关性     

黄利权  施明华  蔡荣湘 《浙江大学学报(农业与生命科学版)》1995,(3)

对奶牛隐性乳房炎乳汁中体细胞、乳酸脱氢酶（ＬＤＨ）、碱性磷酸酶（ＡＬＰ）、酸性磷酸酶（ＡＣＰ）、谷氨酸草酰乙酸转氨酶（ＧＯＴ）、谷氨酸丙酮酸转氨酶（ＧＦＴ）和血清白蛋白（ＳＡ）及病原微生物的相关性进行了研究。结果发现ＬＤＨ，ＡＣＰ，ＧＯＴ，ＧＰＴ的活性和ｓＡ的含量变化与白细胞数之间存在极显著的相关关系（Ｐ＜０．０１），尤以ＬＤＨ的相关性最强（ｒ＝０．８５８）；ＡＬＰ与体细胞数之间无相关关系（Ｐ＞０．０５）．不同的乳房炎病原微生物感染乳腺呈现不同的酶象变化，与阴性感染的乳腺相比，各酶的活性均出现增加，尤以金黄色葡萄球菌感染的乳腺有关酶的增加幅度最为显著（Ｐ＜０．０１）。  相似文献   

奶山羊实验性乳房炎病理模型   总被引：4，自引：0，他引：4  

刘涤洁  石伟  庄伟东  冯淇辉  陈杖榴 《华南农业大学学报》2002,23(4):75-77

通过在健康奶山羊一侧乳区内接种1mL(10^6cfu/mL)金黄色葡萄球菌悬液复制乳房炎病理模型，其模型指标有：临床症状，体温变化，乳中体细胞数测定，乳牛乳腺炎电子检测仪检测，病原菌分离培养主病理组织学变化。结果表明按上述方法乳房内接种金黄色葡萄球菌可复制出典型的奶山羊急性乳房炎病理模型。  相似文献   

两种抗菌素油剂防治奶牛干奶期乳房炎的疗效比较     

方维焕  裘志明 《浙江大学学报(农业与生命科学版)》1990,(3)

以杭州乳房炎诊断法(HMT)、细菌学检验和临床检查结果为依据,综合判断PC-805和TA-125两种抗菌素油剂对干奶期乳房炎的防治效果。66头受试奶牛随机分成两组,第一组35头136个乳区于停奶当天经由乳头管注入20ml PC-805;第二组31头121个乳区以同样方法注入20ml TA-125。第一组牛在干奶前有72个乳区为HMT强阳性(++、+++),至产后降为11个,转阴率为84.7％;第二组牛在干奶前HMT强阳性乳区为66个,产后降至6个,转阴率为90.9％。第一、二组HMT强阳性乳区的细菌转阴率分别为57.4％和68.4％,除去新感染后则分别为70.5％和75.4％。这两种制剂对链球菌感染的效果较好,而对葡萄球菌的作用较差,第一组牛在千奶期间的临床发病率为2.9％,第二组为1.7％。尽管TA-125的疗效较好,但统计结果表明这两种制剂对HMT和细菌转阴率以及临床发病率方面均无显著差异(P>0.05)。  相似文献   

奶牛乳腺炎分离细菌裂解苗免疫试验   总被引：1，自引：0，他引：1  

王赟  靳亚平  林炜明 《西北农林科技大学学报(自然科学版)》2004,32(6):32-34,40

应用自奶牛乳房炎检测为阳性的牛乳汁中分离的4种优势G+菌,分别与葡聚糖、油佐剂和FIA3种佐剂制成细菌裂解苗免疫家兔。于免疫后第7天开始,每隔7d采集心脏血,分离血清,以试管凝集试验检测血清中抗体效价,以此评价所分离细菌的免疫原性和所用佐剂的免疫促进作用,筛选适宜的佐剂。结果显示,所分离的优势G+菌刺激家兔机体后可产生高效价的抗体,免疫后第7天,抗体效价开始明显上升,第28天左右达到峰值,并一直持续到试验结束(63d)。比较3种佐剂的免疫促进效果,以FIA效果最好,但3种疫苗间的免疫效果差异不显著,而与对照组相比,差异均达极显著(P<0.01)。  相似文献   

宫炎净对奶牛乳房炎的治疗效果研究     

黄利权  蔡荣湘  施明华 《浙江大学学报(农业与生命科学版)》1997,(1)

应用宫炎净对奶牛临床型乳房炎、隐性乳房炎进行了治疗研究．结果表明，宫炎净治疗临床型乳房炎具有明显的效果，治愈率高达８４．６％，高于青、链霉素的治疗效果（７５．０％）．通过对病原菌的分离、鉴定表明，宫炎净对乳房炎病原菌有显著的抑杀作用，对链球菌类病原菌效果更佳．对宫炎净治疗后的隐性乳房炎乳汁在停药后１ｄ、３ｄ进行分析，发现宫炎净可明显降低乳汁内的体细胞数、乳酸脱氢酶、谷氨酸丙酮酸转氨酶的活性和血清白蛋白的含量，提示了宫炎净治疗后乳腺损伤组织的修复过程  相似文献   

奶牛酵母菌性乳腺炎的诊治试验   总被引：1，自引：0，他引：1  

曹思军  刘有旺  朱瑞良 《安徽农业科学》2008,36(21)

[目的]采用中药方剂治疗经多种抗生素治疗无效的奶牛乳腺炎。[方法]从牛奶中分离病原真菌,进行药敏试验,确诊后使用自制"乳消汤"开展临床治疗。[结果]20例奶牛中,19例确诊为酵母菌性乳腺炎,经治疗后除1例热带念珠菌病例外全部痊愈。[结论]该研究中自制中药方剂"乳消汤"可用于酵母菌性乳腺炎的治疗。  相似文献   

Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis     

Song Xue  Li De-jun  Feng Guo-feng  Li Bei  Liu Yun-feng 《东北农业大学学报(英文版)》2015,(1)

Subclinical endometritis is a physiological inflammation that serves to clear persistent contaminants from the uterus. To investigate the alteration of antioxidant, such as vitamin E(VE) and vitamin C(VC), total oxidant capacity(TOC) and nitric oxide(NO) in cows with normal and subclinical endometritis(SCE), we examined the concentrations of NO, VC and VE, TOC and polymorphonuclear neutrophils(PMN) percentage in uterine secretion. The cows were divided into two groups, normal(n=20) and subclinical endometritis(SCE, n=60), based on endometrial cytology(presence of PMN≥5%). Uterine secretion and blood were collected as described previously. Griess reaction was used to determine the concentration of NO. The concentrations of TOC, VC and VE were detected by a commercially available assay kit. The results showed that the concentrations of NO, TOC and PMN percentage were significantly higher(P0.01, P0.05, and P0.01, respectively) in uterine secretion with SCE compared to those from normal; however, the levels of VC and VE were significantly lesser(P0.01). In conclusion, the concentrations of NO, TOC, VC, VE and PMN percentage differed between normal and SCE cows. Meanwhile, the relationship between the concentration of NO and PMN percentage from uterine secretion in cows with subclinical endometritis were positively correlated. Consequently, these alterations in NO, TOC, VC, VE levels and PMN percentage contributed to as a diagnostic index of the uterine inflammation, with the aim to increase the reproduction of the cows and the decrease economic losses.  相似文献   

中药涂剂治疗波尔羊临床乳房炎的疗效     

钱立  丁建平  李志明  章孝荣  葛凯  马兆臣  章美林 《青海畜牧兽医杂志》2006,36(4):24-25

经确诊为临床型乳房炎的96只波尔羊作为研究对象,分为两组,每组48只,分别采用纯中药涂剂和青、链霉素治疗。试验结果显示:纯中药涂剂治疗临床型乳房炎波尔羊48例,治愈37例,治愈率77%,好转9例,无效2例,总有效率95.8%;用青、链霉素共治疗48例,治愈28例,治愈率58.3%,好转12例,无效8例,总有效率83.3%。经检验,纯中药涂剂对波尔羊临床乳房炎的治愈率、总有效率明显高于青、链霉素(P<0.05)。  相似文献   

Potential of differential somatic cell counts as indicators of mastitis in quarter milk samples from dairy cows     

U Emanuelson  P Wever 《Acta veterinaria Scandinavica》1989,30(4):475-481

Bacteriological status, somatic cell counts and proportions of lymphocytes, granulocytes and monocytes were determined in 1,659 quarter milk samples from 39 dairy cows. Discriminant analysis was performed in order to assess the ability of total and differential somatic cell counts and combinations of total somatic cell count and each of the differential cell counts, to discriminate between infected and pathogen-free quarters, as well as between quarters infected with minor pathogens and quarters infected with major pathogens. Total somatic cell count classified 82.9% of all quarters correctly with respect to bacteriological status. Differential somatic cell count was less effective than total somatic cell count in discriminating between infected and pathogen-free quarters, as well as between quarters infected with minor vs. major pathogens. Combination of total and differential somatic cell counts did not improve the rate of correctly classified quarters. Inclusion of demographic data into the discriminant function increased the number of quarters correctly classified, mainly through an increase in the proportion of correctly classified infected quarters.  相似文献   

奶牛乳房炎发泡药剂的研究Ⅱ.发泡药剂的研制与动物试验     

皮宝安  王铭杰等 《黑龙江畜牧兽医》2003,(1):10-11

研究了奶牛乳房炎发泡药剂的制备方法及其质量控制标准。该药剂是以碳酸氢钠和枸橼酸作发泡剂，研制药物的发泡量为原药液体积的8-12倍。经动物乳房给药试验证实，该药剂安全、无刺激性、在乳腺内分布效果明显优于常规剂型，室温保存有效期为2年。  相似文献