小麦V-CMS和杂种F1代线粒体蛋白质组表达差异     

郭宝健  李鹏  牟秋焕  刘保申 《分子植物育种》2011,9(3):302-308

本研究以V型小麦恢复基因的近等基因系为基础材料,运用蛋白质组学技术对V-CMS及杂种F1黄化苗和幼穗时期线粒体蛋白质表达图谱进行了比较,分析了差异蛋白质点与CMS的关系.主要结果如下:(1)比较了黄化苗和幼穗线粒体蛋白质的表达图谱,鉴定了10个差异表达的蛋白质点,包括与能量代谢有关的F0-F1ATP酶复合物的亚基和一些...  相似文献   

Interference elimination of PCB and its ramification in determining trace PBBs/PBDEs by GC/MS     

HUA Li  ZHANG Hong-quan  ZHANG Lei  LIU Tao  HUANG Yuan-qiao 《保鲜与加工》2011,(7):137-146

The normal method to determine the trace brominated flame retardants such as polybrominated biphenyls (PBBs) and polybrominated diphenylethers (PBDEs) contained in the electronic and electrical products is gas chromatography/mass spectrometry (GC/MS). However, the method is based on the determinations of the persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs), and polychlorinated naphthalene (PCNs) etc. The interferences of those compounds are inevitable. Therefore, in order to overcome this problem, two methods e.g. 1) The combination use of gas chromatography negative ion chemical ionization mass spectrometry (GC-NICI-MS) and gas chromatography-electron impact-mass spectrometry (GC-EI-MS) is employed, 2) a matrix solid-phase dispersion (MSPD) method with YMC ODS-C18 as carrier is developed. The result show that, by the former method, the brominated isomers or ramifications are distinguished remarkably from other halogen compounds because that anion fragment retention peaks of ［Br］-, ［HBr2］- and molecule chain fragment retention peaks of ［M+2］+,［M+4］+,［M+6］+,［M+8］+ are observed simultaneously, and thus the selectivity to determine bromine-containing retardant flames is greatly improved. Using the latter method, the gas chromatographic peaks of multiple polychlorinated biphenyls and polybrominated diphenylethers can be efficiently separated. Thus provides a project to solve interferences of POPs in brominated flame retardants’ determinations. The standard addition experimental results of 10 kinds of BDEs/PCBs belonging to 8 sorts of electronic and electrical equipment show this method has a high precision and reliability due to 60%～98% recovery and <9.5% relative error, which meet the needs regulated by the IEC Commission. It provides a technical support for electronic and eletrical industries in China to further comply with RoHS directive.  相似文献   

火焰原子吸收光谱法测定播娘蒿中微量元素含量   总被引：1，自引：0，他引：1  

肖忠峰  韩岩君  党艳秋  李普明  赵西梅  于祥  刘京涛  田家怡 《安徽农业科学》2007,35(33):10575-10576

采用火焰原子吸收光谱法测定了播娘蒿中4种微量元素的含量。结果表明,播娘蒿中含有较丰富的铁、锌、锰、铜等微量元素,其含量分别为136.50、39.762、9.518、.11μg/g,具有较高的开发利用价值。  相似文献   

加热对牛奶中风味物质的影响   总被引：4，自引：0，他引：4  

杨楠  张兰威 《东北农业大学学报》2007,38(1):39-43

试验用有机溶剂乙醚萃取了原料乳和巴氏杀菌乳中的风味物质,并用气相色谱(GC)-质谱(MS)联用法结合内标法对其进行定性和相对定量分析。结果表明,在原料乳的风味物质中,共鉴定出46种物质,主要由C_8～C_(20)的酮类、烷烃、烷酸和酯类组成;在加热温度为65℃时间为30 min的巴氏杀菌乳中,共鉴定出69种物质,除上述几类物质外还含有醛类、酯类和烯类等物质。其中辛烷、环氧乙烷、己酸、苯乙酮、壬醛、辛酸、十二烷、2-癸醛、十三烷、2,4(E)(E)-十二烯醛、2-亚甲基-环戊醇、癸酸等具有较强的挥发性,是牛乳中风味物质中的重要成分。  相似文献   

A flexible approach to managing variability in grain yield and nitrate leaching at within-field to farm scales     

M. T. F. Wong  S. Asseng  H. Zhang 《Precision Agriculture》2006,7(6):405-417

We up-scaled the APSIM simulation model of crop growth, water and nitrogen dynamics to interpret and respond to spatial and temporal variations in soil, season and crop performance and improve yield and decrease nitrate leaching. Grain yields, drainage below the maximum root depth and nitrate leaching are strongly governed by interaction of plant available soil water storage capacity (PAWC), seasonal rainfall and nitrogen supply in the water-limited Mediterranean-type environment of Western Australia (WA). APSIM simulates the interaction of these key system parameters and the robustness of its simulations has been rigorously tested with the results of several field experiments covering a range of soil types and seasonal conditions in WA. We used yield maps, soil and weather data for farms at two locations in WA to determine spatial and temporal patterns of grain yield, drainage below the maximum root depth and nitrate leaching under a range of weather, soil and nitrogen management scenarios. On one farm, we up-scaled APSIM simulations across the whole farm using local weather and fertiliser use data and the average PAWC values of soil type polygons. On a 70 ha field on another farm, we used a linear regression of apparent soil electrical conductivity (EC_a) measured by EM38 against PAWC to transform an EC_a map of the field into a high resolution (5 m grid) PAWC map. We then used regressions of simulated yields, drainage below the maximum root depth and nitrate leaching on PAWC to upscale the APSIM simulations for a range of weather and fertiliser management scenarios. This continuous mapping approach overcame the weakness of the soil polygons approach, which assumed uniformity in soil properties and processes within soil type polygons. It identified areas at greatest financial and environmental risks across the field, which required focused management and simulated their response to management interventions. Splitting nitrogen applications increased simulated wheat yields at all sites across the field and decreased nitrate leaching particularly where the water storage capacity of the soil was small. Low water storage capacity resulted in both low wheat yields and large leaching loss. Another management option to decrease leaching may be to grow perennial vegetation that uses more water and loses less by drainage.Paper from the 5th European Conference on Precision Agriculture (5ECPA), Uppsala, Sweden, 2005  相似文献   

完熟金煌芒果实香气成分研究   总被引：1，自引：0，他引：1  

魏长宾  武红霞  马蔚红  王松标  孙光明 《广西农业科学》2007,38(4):443-445

采用顶空固相微萃取技术和气相色谱-质谱联用仪对完熟金煌芒果实的香气成分进行初步研究,结果表明,共检出22种香气成分,主要的香气成分有E-3-溴-2,6,10,15,19,23-六甲基-6,10,14,18,22-十一碳五烯-2-醇(相对含量20.57%),3-蒈烯(相对含量11.50%)等;主要的香气类型有醇(相对含量25.76%),酸(相对含量24.42%),萜烯类(相对含量18.18%)等。  相似文献   

近视眼患者头发中钙、镁、锌、铜含量检测分析   总被引：2，自引：0，他引：2  

臧凤艳  王瑞 《天津农学院学报》2006,13(1):20-21,26

用原子吸收法测定了45例近视眼患者及34例正视眼者头发中Ca、Mg、Zn、Cu元素的含量。结果表明:近视眼患者头发中Zn、Cu、Ca含量显著低于正视眼(P<0.05),Mg含量与正视眼者差异不显著。揭示人体内Zn、Cu、Ca元素含量与近视眼发病存在一定关系。  相似文献   

水浸提制备待测液火焰光度法测定牡丹叶中钾含量的方法研究     

成玉梅 《河南科技大学学报(农学版)》2001,21(2):115-116

烘干、磨碎、过40目筛的牡丹叶样品，用水浸提法、醋酸铵浸提法及硫酸消化法制备待测液，火焰光度法测定牡丹叶钾的百分含量分别为0．978，9．0825，0．9788，显著性检验结果表明三者相关不显著，说明在测定牡丹叶含量时，可以用水浸提法制备待测液。  相似文献   

天2井原油生物标记化合物与油源研究     

唐友军  文志刚  王明清  张超漠  李军 《长江大学学报》2008,5(2)

采用定量气相色谱质谱技术,剖析了天2井原油的生物标志物组合特征并追踪了其油源。研究结果表明,天2井原油中C31-C35藿烷丰度依次降低,含有较高丰度的伽马蜡烷,无重排藿烷和重排甾烷,C27-C29规则甾烷呈反“L”型分布,C28甾烷丰度较高。甾烷的异构化成熟度参数、甲基菲指数均暗示原油成熟度低。在对本区可能烃源岩生物标志物组合特征广泛调查的基础上,从生物标志物指纹和绝对浓度特征确认了天2井原油与下白垩统巴音戈壁组下段烃源岩有成因联系,指出巴音戈壁组下段烃源岩为天草凹陷主力烃源岩。  相似文献   

昌黎原产地域赤霞珠干红葡萄酒香气成分研究   总被引：6，自引：0，他引：6  

李华  李佳  王华 《西北农林科技大学学报(自然科学版)》2007,35(6):94-98

为了对我国葡萄酒实施原产地域保护提供理论依据。采用溶剂微萃取法提取赤霞珠干红葡萄酒的香气成分,用内标法对检测峰进行半定量分析,经气相色谱-质谱并结合计算机检索技术对分离化合物进行鉴定。结果表明,赤霞珠干红葡萄酒中共分离出43个峰,鉴定出35种香气物质,其中醇类、酸类、酯类分别为8,9,14种,占赤霞珠干红葡萄酒香气成分总量的52.12%,12.32%,33.52%;香气成分相对含量排在前10位的分别为:苯乙醇、琥珀酸单乙酯、异戊醇、琥珀酸二乙酯、辛酸、2-甲基-1-丁醇、五氧四氢呋喃-2-羟基乙酯、2-羟基-3-甲基-琥珀酸二乙酯、3-甲硫基-1-丙醇、香草酸乙酯。35种香气成分的相对标准偏差均在10以下。说明采用的溶剂微萃取法准确度高,分析结果可靠,能真实反映葡萄酒香气成分的组成,适用于葡萄酒香气成分分析。  相似文献