动物鲜味受体的研究进展及其基因表达调控     

王晶  张勇  黄铁军 《动物营养学报》2016,(9):2702-2708

动物的鲜味受体包括代谢型谷氨酸受体(m GluR)和味觉受体异源二聚体(T1R1/T1R3),是C型G蛋白偶联受体,N末端捕蝇草模块(VFT)区域可与鲜味配体结合,识别鲜味。本文主要论述了鲜味受体的研究进展、鲜味识别转导机制及鲜味受体基因的表达调控等,以期为相关研究提供参考。  相似文献   

山豆根多糖对鸡脾脏淋巴细胞信号转导相关分子水平的影响   总被引：2，自引：0，他引：2  

帅学宏  苏子杰  胡庭俊  曾芸  韦英益  李跃华 《动物医学进展》2010,31(1):36-41

为探讨山豆根多糖(SSP)对体外培养的鸡脾脏淋巴细胞信号分子的影响,采用终浓度为50、100、200、400μg/mL的山豆根多糖分4、8、12、24 h 4个时间段刺激培养鸡脾脏淋巴细胞,测定培养上清或细胞内环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、6-酮-前列腺素F1α(6-keto-PGF1α)、血栓素B2(TXB2)和一氧化氮(NO)信号转导分子水平。结果表明,山豆根多糖在终浓度为100μg/mL时与鸡脾脏淋巴细胞共同培养8 h后能显著升高cAMP(P0.01)、6-keto-PGF1α(P0.05)、TXB2(P0.01)水平,4种浓度的山豆根多糖对NO分子水平随时间的变化有不同程度的升高(P0.05),影响cAMP/cGMP和6-keto-PGF1α/TXB2信号体系。提示山豆根多糖可通过影响免疫细胞内的信号转导而影响机体的免疫功能。  相似文献   

小型猪特异性麻醉颉颃剂对大鼠脑NO/cGMP信号转导系统的动态影响     

卢德章  范宏刚  王洪斌  张栾松  马昆  姜胜  谭丽娟  于世明 《中国兽医杂志》2012,48(5):10-13

为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。  相似文献   

栀子花化学成分的GC/MS分析   总被引：2，自引：0，他引：2  

张国良  沈国军  凌国海  张王印 《中国园艺文摘》2012,(11):13-14,66

采用超声波提取栀子花提取液,然后对其化学成分进行GC/MS分析。从栀子花中分离确定出27种主要成分,其中萜类物质含量相对较高。  相似文献   

Characterization of mutations in the two-component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in the os-1 mutants of Neurospora crassa     

Ochiai N  Fujimura M  Motoyama T  Ichiishi A  Usami R  Horikoshi K  Yamaguchi I 《Pest management science》2001,57(5):437-442

Osmotic-sensitive (os-1) mutant alleles in Neurospora crassa exhibit resistance to dicarboximides, aromatic hydrocarbons and phenylpyrroles. We have previously reported that the os-1 mutants can be classified into two groups based on their resistance to fungicides and osmotic stress: type I, which are highly resistant to iprodione and fludioxonil but moderately sensitive to osmotic stress, and type II, which are highly sensitive to osmotic stress but moderately resistant to fungicides. To explain the mechanism of resistance to these fungicides, we cloned and sequenced the mutant os-1 genes that encode putative osmo-sensing histidine kinase. Within the os-1 gene product (Os1p), the type I strains, NM233t and Y256M209, carried a stop codon at amino acid position 308 and a frameshift at amino acid position 294, respectively. These mutation sites were located on the upstream of histidine kinase and the response regulator domains of Os1p, strongly suggesting that type I strains are null mutants. The null mutants, NM233t and Y256M209, were highly resistant to iprodione and fludioxonil; thus Os1p is essential for these fungicides to express their antifungal activity. The amino acid changes in Os1p, 625Pro from Leu, 578Val from Ala, and 580Arg from Gly were found in the type II strains, M16, M155-1 and P5990, respectively. Os1p is novel in having six tandem repeats of 90 amino acids in the N terminal. Each amino acid change of the type II strains was located on the fifth unit of six tandem repeats. Type II strains with single amino acid changes were more sensitive to osmotic stress than the null mutants (type I), indicating that the amino acid repeats of Os1p were responsible for an important function in osmo-regulation.  相似文献   

荔枝霜疫霉中双组分信号转导系统的鉴定与表达分析     

王荣波  陈姝樽  刘裴清  李本金  翁启勇  陈庆河 《植物病理学报》1955,50(1):49-59

 荔枝霜疫霉(Peronophythora litchii)隶属于茸鞭生物界卵菌门疫霉属,由其引起的荔枝霜疫病是目前荔枝生产上一种最重要的病害,严重影响荔枝产量和鲜果品质。双组分信号途径在微生物中参与多种生命活动,但是在卵菌中尚未有相关研究。本研究通过生物信息学分析在荔枝霜疫霉中鉴定到2个杂合型组氨酸激酶(PlHK1、PlHK2)和1个响应调控蛋白(PlRR1)。其在卵菌中是保守存在的,并且与真菌在进化上相对独立。功能域分析表明,PlHK1和PlHK2的C端额外的融合了1个磷酸转移功能域(Hpt),这与真菌和植物的存在显著差异。转录分析表明3个基因在荔枝霜疫霉侵染阶段上调表达,并且响应渗透胁迫和氧化胁迫。以上结果揭示了双组分信号系统可能在疫霉致病过程中发挥重要作用。  相似文献   

支链氨基酸对mRNA翻译起始的调控作用     

刘兆金  王彬  黄瑞林  印遇龙 《家畜生态》2007,(1)

综述了支链氨基酸(BCAAS)对mRNA翻译起始过程的调控及其在调控过程中信号转导机制方面的研究进展,包括翻译的起始过程,BCAAs对翻译起始过程的调节和BCAAs在调节起始过程中的信号转导机制,并就值得深入研究的问题进行了探讨。  相似文献   

Xaf1 regulates TNFR signal pathway and induces apoptosis     

XIA Yan  TAN Wei-ping  LIANG Li-yang  SU Hao-bin  GUO Hai-xia  FANG Jian-pei  HUANG Shao-liang 《园艺学报》2007,23(7):1347-1351

  相似文献   

不同脱水程序在乙型病毒性肝炎患者肝穿刺组织中应用的比较     

曾敏 《农业环境科学学报》2013,1(5):608-609

目的 探讨乙型病毒性肝炎患者肝穿刺组织最合适的快速石蜡脱水程序.方法 随机选取乙型病毒性肝炎肝穿刺标本50例,每一例穿刺标本随机取三份新鲜组织,取材固定后,分为三组,A组为快速石蜡处理3 min组、B组为快速石蜡处理5 min组、C组为快速石蜡处理10 min组.快速石蜡切片应用超声波技术结合水浴加温及应用BT生物组织透明剂代替二甲苯作为透明剂.三组所做切片同时进行HE染色.结果 快速石蜡处理3 min组所制的片优良率为0%,快速石蜡处理5 min组、10 min组所制的片优良率为100%,χ2=150,P〈0.001,三组的优良率差异有统计学意义.结论 应用JYCL-2 超声组织处理仪＋BT 生物组织透明剂处理肝组织5-10 min就能满足肝组织快速临床病理诊断的需要,缩短了发报告的时间,同时也符合实验室环保的要求.  相似文献   

Preliminary telemetry data on the movement patterns and habitat use of European catfish (Silurus glanis) in a reservoir of the River Ebro, Spain     

J. Carol  L. Zamora  E. García-Berthou 《Ecology of Freshwater Fish》2007,16(3):450-456

Abstract –  Knowledge of the movements and diel behaviour of the European catfish ( Silurus glanis ), the largest European freshwater fish, is limited to anecdotal information. In a preliminary telemetry study of European catfish, the spring diel movement patterns of five adult catfish were examined. After intraperitoneal insertion of the acoustic tags, the positions of the fish were recorded automatically in the Flix Reservoir (River Ebro, NE Spain). A marked nocturnal mobility pattern was observed throughout the study. During daytime, the catfish were consistently located in the littoral zone and spent extended periods of the day hidden in concealed habitats. Catfish movements were in a radial pattern, with upstream and downstream excursions followed by returns to a previously occupied location. Significant individual variations in movement pattern were observed among the tagged fish and within the 24 h cycle for each fish. Mean instantaneous swimming speed was 0.17 body lengths per second (BL·s⁻¹) at night but 0.09 BL·s⁻¹ during the daytime.  相似文献