西瓜维管束真菌区系研究     

于思勤  王守正 《河南农业大学学报》1992,26(4):380-387

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不同田间配置方式对水稻穗颈组织的影响   总被引：6，自引：0，他引：6  

荆彦辉  徐正进  王建林 《沈阳农业大学学报》2003,34(5):355-357

用3个水稻品种在两种行距和两种穴苗数的不同田间配置方式下，观察水稻穗颈组织性状。结果表明，颈横切面积、颈壁面积、总维管束面积3个性状。在不同品种间存在显差异，不同田间配置方式对穗颈组织影响较小。  相似文献   

Effect of concentrated decoction of Chinese herbal compound Buyanghuanwutang on cAMP-PKA-CREB signaling pathway in hippocampus of rats with vascular dementia     

ZHANG Hong-bo  GAO Wei-juan  QIAN Tao  TANG Jing-long  LI Jun 《园艺学报》2011,27(4):718-721

AIM: To evaluate the role of concentrated decoction of Chinese herbal compound Buyanghuanwutang (BYHWT) in cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)-cAMP response element-binding protein(CREB) signaling pathway in hippocampus of rats with vascular dementia (VD). METHODS: The rats were randomly divided into sham operation group (sham-operated rats treated with normal saline), VD model group (VD rats treated with normal saline), BYHWT treatment group (VD rats treated with BYHWT) and nimodipment treating group (VD rats treated with nimodipine). The rat model of VD was build by the method of four-vessel occlusion. The rats in all 4 groups were administered with the corresponding reagents for successive 30 days. The content of cAMP was measured by radioimmunoassay. The expression of PKA catalytic subunit (PKAc) was observed by Western blotting. The changes of DNA-binding activity of CREB in rat hippocampus were detected by electrophoretic mobility shift assay. RESULTS: The content of cAMP, the expression of PKAc and the DNA-bingding activity of CREB in the hippocampus of VD rats were lower than those in the hippocampus of sham-operated rats (P<0.01). The above indexes in both nimodipine treatment group and BYHWT treatment group were definitely higher than those in VD model group (P<0.01). CONCLUSION: BYHWT increases the content of cAMP, the expression of PKAc and the DNA-binding activity of CREB in VD rat hippocampus, thus strengthening the cAMP-PKA-CREB signaling pathway.  相似文献   

Inhibitory effect of 1,25-(OH)₂D₃ on proliferation of airway smooth muscle cells from passively-sensitized patients     

SONG Ying-fang  LAI Guo-xiang  QI Hao-wen  WU Chang-gui 《园艺学报》2011,27(9):1828-1831

AIM: To investigate the effects of 1,25-dihydroxyvitamin D₃ on the proliferation of passively-sensitized human airway smooth muscle cells (HASMCs), and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)₂D₃ was used as the interventor.The effect of 1,25-(OH)₂D₃ on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by flow cytometry.The expression of proliferating cell nuclear antigen (PCNA) was measured by the method of immunocytochemical staining.RESULTS: 1,25-(OH)₂D₃ at the concentrations of 10^-9-10^-7 mol/L markedly inhibited the cell proliferation and the maximum effect was observed at the concentration of 10^-7 mol/L.This concentration of 1,25-(OH)₂D₃ markedly suppressed the PCNA-positive rate and hampered the G₁/S transition in HASMCs passively-sensitized by asthmatic serum.CONCLUSION: 1,25-(OH)₂D₃ has direct inhibitory effects on the proliferation of passively-sensitized HASMCs in vitro, which may be concerned with the beneficial role of 1,25-(OH)₂D₃ on the prevention and therapy of asthmatic airway remodeling.  相似文献   

Contribution of hypoxia‐inducible factor‐1α to transcriptional regulation of vascular endothelial growth factor in bovine developing luteal cells     

Zhenghong ZHANG  Dingzhong YIN  Zhengchao WANG 《Animal Science Journal》2011,82(2):244-250

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K_(Ca)3.1通道与血管功能的研究进展     

杨柳 《畜牧与饲料科学》2011,32(5)

研究表明,在促进血管疾病发展的众多细胞类型中,如T细胞、B细胞、内皮细胞、成纤维细胞、巨噬细胞以及去分化的平滑肌细胞,KCa3.1都起到了一定的作用。生理上,KCa3.1已被证明在乙酰胆碱及内皮源性超极化因子(EDHF)介导下发生超极化,从而发挥控制血压的作用。病理上,KCa3.1异常则可导致血管平滑肌细胞增殖迁移。因此,从KCa3.1在内皮细胞及平滑肌细胞两方面对其作用作一总结,并对KCa3.1通道的研究进展进行综述。  相似文献   

Evaluation of a wild lentil collection for resistance to vascular wilt     

B. Bayaa  W. Erskine  A. Hamdi 《Genetic Resources and Crop Evolution》1995,42(3):231-235

Vascular wilt caused byFusarium oxysporum f. sp.lentis Vasud. & Srin. is the major disease of the cultivated lentil (Lens culinaris Medikus). Host plant resistance is the most practical method of disease management. Wild lentils represent an unexplored potential source for disease resistance and other characters. Screening 219 accessions of wildLens Miller and 2 accessions ofVicia montbretii Fisch. & Mey. (syn.Lens montbretii (Fisch et Mey) Davis et Plitm.) for resistance to a Syrian isolate of this fungus at the seedling stage was conducted under artificial inoculation in a plastic house. Resistance at the reproductive growth stage was confirmed in pots in a plastic house and in a wilt-sick plot. Three accessions each ofLens culinaris ssp.orientalis (Boiss.) Ponert andL. nigricans M.B. Godr. ssp.nigricans Godr. and 2 ofL. nigricans ssp.ervoides (Brign.) Lad. maintained their resistance at the reproductive growth stage in the plastic house. All accessions ofL. culinaris ssp.odemensis Lad. andV. montbretii were susceptible. However, in the sick-plot only three accessions (ILWL 79 & ILWL 113 ofL. culinaris ssp.orientalis and ILWL 138 ofL. nigricans ssp.ervoides) maintained a good level of resistance. Resistance at the seedling stage was often found in accessions collected from northern and western sites of the distribution of the genus at low elevations. The most resistant accessions in the field at the reproductive growth stage were from Syria and Turkey.  相似文献   

L-精氨酸对肺动脉高压肉鸡肺小动脉平滑肌细胞凋亡的影响   总被引：5，自引：1，他引：4  

谭勋  刘艳娟  李锦春  潘家强  孙卫东  王小龙 《畜牧兽医学报》2005,36(9):937-941

将240羽14日龄艾维茵-2000商品代肉鸡于14d时随机等分为常温对照组（NT组），低温组（LT组）和低温加L-精氨酸组（LA），白14d起，LT和LA组舍内温度从28℃以1～2℃／d的速度下降，至21d降至12～14℃，并维持到试验结束。此外，LA组自14d起在饲料中按100mg／kg剂量添加L—Arg，直至试验结束。记录肺动脉高压综合征（PHS）发病率，并分别于24、32、39和45d测定右心室／全心室质量比（RV／TV）、血浆NO、肺小动脉管壁面积／管总面积（WA／TA）和平均中膜厚度（mMTPA），并用原位缺口末端标记法（TUNEL法）对肺小动脉凋亡平滑肌细胞进行染色和计数。结果：LT组肉鸡PHS发病率、RV／TV、mMTPA和WA／TA值较NT组升高（P〈0．05）；LA组肉鸡PHS发病率较LT组降低（P〈0．05），mMTPA和WA／TA值与LT组相比呈下降趋势，在32d时显著降低（P〈0．05）；LT组血浆N0浓度总体上与NT组差异不显著（P〉0．05），仅在39d时降低（P〈0．05），而LA组血浆N0浓度自32d时起较NT和LT组显著升高（P〈0．05）；LA组肉鸡肺小动脉平滑肌凋亡细胞百分率较NT组和LT组均显著升高（P〈0.05），但LT组与NT组之间相比无显著差异（P〉0．05）。上述结果表明，L-Arg／NO通过促进肺小动脉平滑肌细胞凋亡，从而在一定程度上抑制了肺血管重构的形成。  相似文献   

Effects of three kinds of calcium activator on the proliferation of cultured vascular smooth muscle cells in rats     

YANG Yong-jian  ZHANG Xin  ZHOU Xin-wen  ZHU Jun  ZHAO Long-sheng 《园艺学报》2001,17(12):1160-1162

AIM: To investigate the effects of intracellular free calcium ([Ca²⁺]i) from different resources on the proliferation mediated by mitogen activated protein kinase (MAPK) in vascular smooth muscle cells (VSMCs). METHODS: Cultured VSMCs were used in all experiments. Calcium influx was stimulated by angiotension Ⅱ(Ang Ⅱ). The release of intracellular calcium stores was induced by inositol trisphosphate (IP₃) and ryanodine (RY). MAPK activity was measured by [γ-³²P]-ATP incorporation MAPK protein expression by western blot, VSMCs proliferation by [³H]-Leucine ([³H]-Leu) and [³H]-Thymidine ([³H]-TdR) incorporation. RESULTS: Compared to the control VSMCs, Ang Ⅱ, IP₃ and RY significantly increased [Ca²⁺]i concentration activity of MAPK and its protein content in VSMCs. The promotion of [³H]-Leu and [³H]-TdR incorporation in VSMCs was also observed (P<0.01). CONCLUSION: The study indicated that calcium activator-induced increase in the activity and protein content of MAPK was involved in the proliferation of VSMCs, which was closely related to the [Ca²⁺]i concentration but independent to its origin.  相似文献   

An experimental exploration of focal inflammatory response in cerebral ischemic foci in the rat     

LEI Wan-long  LIU Yong  YUANG Qun-fang  YAO Zhi-bin  LING Feng-dong 《园艺学报》2001,17(3):271-274

AIM: To study the pathological relationship of vascular cell adhesion molecule-1 (VCAM-1) expression and monocyte/macrophage infiltration with focal brain ischemia. METHODS: Immunohistochemical technique and focal brain ischemia/reperfusion model were used in the study in order to explore profiles and time-course of VCAM-1 expression and monocyte macrophage (ED2 positive cell) infiltration in ischemic rat brain. RESULTS: VCAM-1 was up-regulated in microvascular endothelial cells in ischemic cortex at 1h postischemia, and continuously expressed during the time of reperfusion. ED2 positive cells infiltrated into ischemic cortex at 1h iscehmia/ 2h reperfusion and then ED2 positive cells increased gradually with the time of reperfusion, ED2 positive cell infiltration showed apparently relationship with VCAM-1 expression, and both of them exhibited the some changes of time-dependence. CONCLUSION: Cerebral ischemia induced VCAM-1 expression and ED2 positive cell infiltration and VCAM-1 may regulate the recruitment of ED2 positive cells in the ischemic brain region. The results suggested that VCAM-1 and ED2 positive cells may be participated in the pathogenesis of cerebral ischemic injury.  相似文献