Evaluation of a percutaneously controlled hydraulic occluder in a rat model of gradual venous occlusion     

Sereda CW  Adin CA  Ginn PE  Farese JP 《Veterinary surgery : VS》2005,34(1):35-42

OBJECTIVE: To evaluate the efficacy of a percutaneously controlled hydraulic occluder (HO) in a rat model of gradual venous occlusion. STUDY DESIGN: Experimental study. ANIMALS: Ten male Sprague-Dawley rats. METHODS: HOs and perivascular transit time ultrasound flow probes were applied to the caudal vena cava (CVC). Occluders in group I rats (n=6) were gradually attenuated over 8 weeks, whereas occluders in group II rats (n=4) were not attenuated. Vena caval blood flow measurements were performed weekly in all rats for 8 weeks. After euthanasia, the CVC was evaluated grossly and by histopathology. RESULTS: Premature occlusion of the vena cava occurred during the 1st week in 3 rats (1 in group I, 2 in group II) because of kinking of the vena cava between the HO and the flow probe. For the remaining rats, mean blood flow in group I decreased significantly from 40.71 mL/min/kg at baseline to 4.68 mL/min/kg at 8 weeks (P=.0094, power=0.91). Group II rats maintained vena caval blood flow at all times during the study. Complete occlusion of the vena cava was confirmed at necropsy in all group I rats. CONCLUSIONS: Gradual occlusion of the CVC of rats was achieved with HOs over 8 weeks. This model is limited by the size of the experimental animals and comparatively large implants. CLINICAL RELEVANCE: HOs may provide a method for gradual occlusion of congenital portosystemic shunts.  相似文献   

籼粳稻维管束性状关系研究     

孙玉友 《黑龙江农业科学》2012,(1):4-6

选用5份粳稻和4份籼稻材料,对不同品种水稻维管束的数量进行比较研究,同时结合程氏指数调查,探讨了籼稻和粳稻之间的维管束差异。结果表明:籼稻穗颈维管束数明显高于粳稻,其大维管束效率较高;籼稻的穗颈维管束数与穗一次枝梗数之比(V/R)也显著高于粳稻,大小维管束比也较高。籼稻程氏打分均较低,而粳稻的较高。  相似文献   

心脉隆注射液对动脉粥样硬化大鼠血管内皮细胞的保护作用   总被引：1，自引：0，他引：1  

张文静  王琳琳  宋树英 《河北北方学院学报(自然科学版)》2012,28(2):79-81

目的探讨心脉隆注射液对动脉粥样硬化（AS）大鼠血管内皮细胞的保护作用.方法采用高脂饲料加维生素D3喂养8周的方法建立动脉粥样硬化大鼠的模型.将大鼠随机分为正常组、模型组、辛伐他汀组、心脉隆治疗组四组,高脂饲料喂养8周后取血测一氧化氮（NO）、内皮素（ET）、前列环素（PGI2）和血栓素（TXA2）的含量.结果心脉隆注射液给药8周后能明显的升高AS大鼠血清NO和血浆PGI2水平（P〈0.05）,显著降低血浆ET的含量（P〈0.05）.结论心脉隆注射液能保护AS大鼠血管内皮细胞,减轻动脉粥样硬化的进程,对动脉粥样硬化有治疗作用.  相似文献   

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its expected roles in the bovine endometrium during gestation     

Mishra B  Kizaki K  Koshi K  Ushizawa K  Takahashi T  Hosoe M  Sato T  Ito A  Hashizume K 《Domestic animal endocrinology》2012,42(2):63-73

Extracellular matrix metalloproteinase inducer (EMMPRIN) and its induced matrix metalloproteinases (MMPs) play a crucial role in tissue remodeling during the peri-implantation period. However, the role of EMMPRIN in the bovine placenta is still unclear. We have postulated that EMMPRIN might play a regulatory role in trophoblastic cell functions during gestation by itself or through the regulation of MMP expression. In this study, EMMPRIN mRNA was detected in the bovine placentome and interplacentome throughout gestation, and its expression was significantly higher in the cotyledon during late gestation. In situ hybridization showed that EMMPRIN mRNA was expressed in the caruncular epithelium and the cotyledonary epithelium, including binucleate cells. Western blot analysis detected a band representing a protein of approximately 65 kDa in the caruncular and cotyledonary tissues, and the intensity of its expression was increased in both of these tissues during late gestation. The expression levels of MMP-2 and MMP-14 in the bovine placenta were higher during late gestation, as was observed for EMMPRIN. Therefore, EMMPRIN might regulate trophoblastic cell functions, especially those of binucleate cells, through MMP expression in the bovine placenta.  相似文献   

Ultrasonographic identification of vascular invasion by adrenal tumors in dogs     

Davis MK  Schochet RA  Wrigley R 《Veterinary radiology & ultrasound》2012,53(4):442-445

Adrenalectomy is the treatment of choice for adrenal tumors that are producing adverse clinical signs. Surgical planning prior to adrenalectomy is aided by identifying tumors with invasion into adjacent vessels or the presence of a tumor thrombus extending into the caudal vena cava. In this paper, we evaluated the sensitivity and specificity of ultrasound in determining if vascular invasion or tumor thrombus is present. Thirty-four dogs with 36 adrenal tumors were reviewed retrospectively. Overall, 36% of tumors had vascular invasion. Abdominal ultrasound was 100% sensitive and 96% specific in identifying the presence of a tumor thrombus in the caudal vena cava. The sensitivity and specificity was 76% and 96%, respectively, when all forms of vascular invasion were evaluated and included patients with vascular wall invasion without concurrent thrombus. Abdominal ultrasound is a good screening tool for identifying vascular invasion or tumor thrombus associated with adrenal tumors in dogs.  相似文献   

高光谱荧光示踪无损检测瓜类作物嫁接苗愈合状态     

谭佐军  蔡霞  阿克拜尔江·卡德尔  余帆  雷红伟  高子艺  杨硕  黄远 《农业工程学报》2023,39(16):276-282

为了减少瓜类嫁接苗的愈合管理时间,实现快速准确判别嫁接苗早期愈合状态,促进嫁接苗规模化生产及育苗产业发展。该研究制备了氮硫掺杂碳点,以该碳点为荧光示踪材料,以西瓜嫁接苗为研究对象,利用高光谱荧光成像方法,探究了西瓜嫁接苗早期愈合状态无损检测的高光谱荧光示踪成像方法。高光谱荧光示踪图像及光谱分析结果表明,利用氮硫掺杂碳点进行荧光示踪,通过高光谱成像仪对瓜类作物早期愈合状态高通量表型的鉴定,能快速、自动、无损地获取嫁接苗愈合的情况。同时,通过氮硫掺杂碳点处理后第12天,处理组的西瓜嫁接苗相较于对照组,根系增长量提升了78.7%,叶面积增长量提升了61.4%。因此,该研究方法可以提早判别嫁接苗的愈合连通,促使瓜类嫁接苗提早移栽,并且氮硫掺杂碳点处理可以促进嫁接苗叶面积和根系的生长,达到种苗壮苗的作用。  相似文献   

Computed tomographic assessment of vascular invasion and resectability of mediastinal masses in dogs and a cat     

WE Scherrer  AE Kyles  VF Samii  EM Hardie  PH Kass  CR Gregory 《New Zealand veterinary journal》2013,61(6):330-333

AIMS: To assess the sensitivity of non-angiographic contrast-enhanced computed tomography (CT) to determine the presence of vascular invasion of cranial mediastinal masses in dogs and a cat, and to evaluate the association between vascular invasion and peri-operative mortality.

METHODS: A retrospective study was conducted on 25 dogs and one cat. CT scans were completed with slices ranging from 2 to 10 mm. CT images were evaluated by a board-certifi ed radiologist blinded to previous diagnoses and surgical fi ndings. Each CT study was evaluated for vascular invasion, defi ned as disruption of the vessel wall and extension of the mass into the vessel lumen. Data retrieved from the surgery reports included surgical approach, whether vascular invasion was present, the surgeon's decision on operability, and post-operative complications.

RESULTS: Computed tomographic evaluation revealed 25/26 masses had no evidence of vascular invasion. During surgical exploration, 10/26 masses were found to invade major regional vasculature; the cranial vena cava (CVC) was the vessel most commonly invaded (7/10 animals), and 4/7 (57%) patients with invasion of the CVC were euthanised or died in the perioperative period, from surgical or disease-related problems, which was signifi cantly higher than patients without vascular invasion (p=0.045).

CONCLUSIONS: Non-angiographic contrast-enhanced CT was signifi cantly less sensitive for detecting vascular invasion of cranial mediastinal masses when compared with surgical evaluation. If the CVC was invaded by a tumour there was a signifi cant risk of death peri-operatively when compared with non-invasive cases.

CLINICAL RELEVANCE: Due to the signifi cantly higher mortality risk associated with invasion of the CVC, a more sensitive method than CT should be investigated to determine vascular invasion of mediastinal masses pre-operatively.  相似文献   

Autologous point‐of‐care cellular therapies variably induce equine mesenchymal stem cell migration,proliferation and cytokine expression     

A. KOL  N. J. WALKER  L. D. GALUPPO  K. C. CLARK  S. BUERCHLER  A. BERNANKE  D. L. BORJESSON 《Equine veterinary journal》2013,45(2):193-198

Reasons for performing study: Autologous cellular therapy products including adipose‐derived stromal vascular fraction (SVF), bone marrow mononuclear cells (BMMNs), cord blood mononuclear cells (CBMNs) and platelet rich plasma are options for treatment of acute orthopaedic lesions while mesenchymal stem cells (MSCs) are culture expanded. These products may contribute to healing by secreting matrix proteins or growth factors, but they may also act on endogenous MSCs to facilitate healing. Objectives: To determine the effects of cell therapy products on MSCs function in vitro. The hypothesis was that cell therapy products promote MSCs functions including proliferation, migration and mediator release. Methods: Fat, bone marrow (BM), cord blood and platelets were obtained from 6 Quarter Horses. The BM‐MSCs and their autologous cell therapy products were co‐incubated in transwells. Mesenchymal stem cells proliferation, migration, gene expression and cytokine concentrations were determined. Results: All cell therapy products increased MSCs proliferation, but SVF induced significantly more proliferation than any other product. Also SVF elicited more MSCs chemotaxis and, along with BMMNs, significantly more MSCs chemoinvasion. Cord blood mononuclear cells stimulated MSCs to produce high concentrations of interleukin‐6 (IL‐6), transforming growth factor‐β1 (TGF‐β1), and prostaglandin E₂ (PGE₂). Stromal vascular fraction and platelet lysate did not stimulate MSCs but SVF and platelet lysate themselves contained high concentrations of PGE₂ and IL‐6 (SVF) and TGF‐β1 (platelet lysate). Conclusions: Autologous cell products variably stimulate MSCs functions with 2 primary patterns apparent. Products either contained preformed mediators that may have intrinsic healing function, or products stimulated MSCs to secrete mediators. Potential relevance: The specific clinical indications for these products may differ to include administration as a sole treatment modality prior to MSCs injection for intrinsic cell and cytokine activity (i.e. SVF) or administration concurrently with MSCs to activate MSCs for treatment of chronic lesions (i.e. CBMNs).  相似文献   

高地钩叶藤节间与节部导管及维管束形态特征径向变异规律   总被引：2，自引：1，他引：1  

汪佑宏  张菲菲  薛夏  季必超  李担  张利萍 《安徽农业大学学报》2019,46(5):806-809

为了更好地了解棕榈藤材的性能、提高我国棕榈藤资源培育和高附加值加工利用水平,选用高地钩叶藤为研究对象,采用显微图像分析系统,对该藤2 m处的节间和节部的导管分子及维管束形态特征进行统计与分析。结果显示,节间与节部大导管分子长度、直径、密度的平均值分别为3 981.363和2 666.883 μm、198.235和202.402 μm、3.611和3.784个·mm^-2;维管束径向直径、弦向直径、密度的平均值分别为523.466和534.794 μm、373.624和379.823 μm、3.078和3.202个·mm^-2。经F检验,仅节间与节部的导管分子长度差异极显著(P＜0.05）。  相似文献   

Host range of Erwinia psidii and genetic resistance of Eucalyptus and Corymbia species to this pathogen     

Nilmara P. Caires  Pollyane S. Hermenegildo  Lúcio M. S. Guimares  Reginaldo G. Mafia  Edival . V. Zauza  Norton Borges Júnior  Jorge L. Badel  Acelino C. Alfenas 《Forest Pathology》2019,49(4)

Dieback caused by Erwinia psidii is currently one of the most important emerging diseases in eucalypt plantations in Brazil. However, little is known in terms of the host range of this pathogen or the potential sources of resistance against the disease it causes. In this study, we inoculated plants of species from nine families to gain insight into the host range of E. psidii. Plants of all inoculated species of Myrtaceae except Acca sellowiana exhibited disease symptoms and therefore represent potential hosts for the pathogen under natural conditions. In addition, the response of four Corymbia species, 29 Eucalyptus species and three interspecific Eucalyptus hybrids to inoculation with E. psidii was evaluated. All Corymbia henryi, Corymbia maculata, Eucalyptus thozetiana, Eucalyptus cloeziana, Eucalyptus viminalis, Eucalyptus dalrympleana and Eucalyptus pilularis plants were highly resistant to the pathogen, whereas differential disease resistance was observed in the other species. This study provides important information on sources of resistance to Erwinia psidii with potential use in the development of clones with enhanced resistance in eucalypt species of economic importance.  相似文献