大豆黄酮对奶牛免疫功能和血清及乳中激素水平的影响   总被引：10，自引：0，他引：10  

杨建英  王艳玲  郭永国  张勇法  兰尊海 《中国畜牧杂志》2006,42(7):15-17

研究口服大豆黄酮对奶牛免疫功能和血清及乳中GH(生长激素)、PR(L催乳素)、S(S生长抑素)水平的影响。结果表明:①大豆黄酮能明显提高血清及乳中特异性抗体水平,表明奶牛整体和乳腺器官的体液免疫功能明显增强;②奶牛血清及乳中GH、PRL含量明显高于对照组,而血清SS含量显著低于对照组。实验结果提示:垂体GH、PRL、和体内SS可能参与了大豆黄酮对奶牛免疫功能的调节过程。  相似文献   

大豆凝集素与大鼠和小鼠小肠黏膜上皮细胞结合规律的研究   总被引：1，自引：1，他引：1  

汤树生  谯仕彦  臧建军 《中国畜牧杂志》2006,42(17):10-13

试验分别选择怀孕的6只SD大鼠和ICR小鼠,单笼饲养。仔鼠于分娩后19日龄断奶,在仔鼠第1、7、14、21、28日龄和第35日龄时分别从6窝仔鼠中每窝随机取1只,每个日龄6只,屠宰后取十二指肠、空肠和回肠制作石蜡切片,免疫组化染色后用图像分析仪检测其阳性染色物的光密度值。结果表明:大鼠和小鼠呈现相似的结合规律,大豆凝集素在十二指肠、空肠和回肠的结合没有显著差异,但不同日龄之间大豆凝集素的结合程度差异极显著(P<0.01)。在大鼠和小鼠出生时结合程度很低,然后逐渐增加,28日龄时达到最高。  相似文献   

Mohs micrographic surgery: a technique for total margin assessment in veterinary cutaneous oncologic surgery     

J. A. Bernstein  E. C. Hodgin  H. W. Holloway  C. S. Hedlund  E. S. Storey  J. D. Hubert 《Veterinary and comparative oncology》2006,4(3):151-160

Mohs micrographic surgery (MMS) is the gold standard for the excision of locally invasive cutaneous malignancies in human dermatological surgery. Using a unique horizontal sectioning technique, MMS enables 100% surgical margin assessment and provides the lowest recurrence rates for locally invasive tumours. The purposes of this preliminary study were to explore the feasibility of application of MMS in the veterinary setting and to establish practical advantages and limitations of its use in a pilot programme. It was hypothesized that MMS technique could provide 100% tumour margin assessment using frozen and/or formalin‐fixed horizontal histopathologic sections. Tumour excision and colour‐coded mapping were performed, and specimen tissue was fixed using either frozen sections or formalin‐fixed sections. Horizontal sections were assessed for quality and presence and location of neoplastic cells based on the mapped orientation. The MMS technique was used in the excision of six squamous cell carcinomas and five mast cell tumours. In all cases, the MMS permitted 100% tumour margins examination.  相似文献   

Veterinary practitioners in rural Australia: a national survey     

Heath TJ  Niethe GE 《Australian veterinary journal》2001,79(7):464-469

OBJECTIVES: To assess the attitudes of Australian veterinarians to issues related to rural veterinary practice. METHODS: A questionnaire was completed by 1367 AVA members, and the data were analysed using the SAS System for Windows 7.0. RESULTS: The country lifestyle and the type of work were seen as major advantages for rural practitioners, and long working hours and after hours demands coupled with sexist attitudes in the case of women, were seen as the main disadvantages. Remuneration, the tiring and often dangerous nature of the work, and social and professional isolation were also seen as major disadvantages. Personal qualities, including the ability to relate to rural people and to fit into rural communities were seen as the most important qualities for a veterinarian in rural practice. The future of rural practitioners was seen to be closely linked to their ability to contribute to the financial viability of the whole animal enterprise and to the fortunes of the rural industries. Companion animals were also seen as a key part of the future work of rural practitioners. CONCLUSIONS: Rural practitioners are characterised by a strong commitment to rural life and work, and they will increasingly be required to contribute to the financial viability of the whole animal enterprise. The full range of professional skills required are likely to be beyond the scope of the undergraduate veterinary course, but the internet and other modern forms of distance education should help practitioners to acquire relevant skills.  相似文献   

An outbreak of equine infectious anaemia in Ireland during 2006: The modes of transmission and spread in the Kildare cluster     

S. J. MORE  I. AZNAR  T. MYERS  D. P. LEADON  T. A. CLEGG 《Equine veterinary journal》2008,40(7):709-711

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ACQUISITION HARDWARE FOR DIGITAL IMAGING     

WILLIAM R. WIDMER 《Veterinary radiology & ultrasound》2008,49(Z1):S2-S8

Use of digital radiography is growing rapidly in veterinary medicine. Two basic digital imaging systems are available, computed radiography (CR) and direct digital radiography (DDR). Computed radiographic detectors use a two‐step process for image capture and processing. Image capture is by X‐ray sensitive phosphors in the image plate. The image plate reader transforms the latent phosphor image to light photons that are converted to an analog electrical signal. An analog to digital converter is used to digitize the electrical signal before computer analysis. Direct digital detectors provide digital data by direct readout after image capture—a reader unnecessary. Types of DDR detectors are flat panel detectors and charge coupled device (CCD) detectors. Flat panel detectors are composed of layers of semiconductors for image capture with transistor and microscopic circuitry embedded in a pixel array. Direct converting flat panel detectors convert incident X‐rays directly into electrical charges. Indirect detectors convert X‐rays to visible light, then to electrical charges. All flat panel detectors send a digitized electrical signal to a computer using a direct link. Charge coupled device detectors have a small chip similar to those used in digital cameras. A scintillator first converts X‐rays to a light signal that is minified by an optical system before reaching the chip. The chip sends a digital signal directly to a computer. Both CR and DDR provide quality diagnostic images. CR is a mature technology while DDR is an emerging technology.  相似文献   

兽用头孢菌素类抗生素研究进展   总被引：1，自引：0，他引：1  

李梅  孙灵灵  袁宗辉  黄玲利 《中国畜牧兽医》2018,45(7):1978-1989

头孢菌素类抗生素具有抗菌谱广、抗菌活性强等特点,在人类医学领域应用十分广泛。在兽医临床中,由于药物种类较少及不规范使用而易使细菌产生交叉耐药性,头孢菌素的应用受到一定程度的限制。文章围绕兽用头孢菌素的发展历程、作用机制、药物代谢动力学与安全性等方面,总结了畜禽专用头孢菌素主要种类、抗菌活性及其在动物体内的药动学参数,比较了兽用头孢菌素的抗菌活性,以及其对敏感菌感染疾病的治疗效果。同时概述了头孢菌素类抗生素耐药性产生机制与耐药现状,并分析比较了各国在此类药物耐药性控制中采取的举措。通过对兽用头孢菌素类抗生素的发展及临床应用进行综述,有利于促进头孢菌素在兽医临床中的科学规范使用。  相似文献   

高效液相色谱法测定6种兽用中药散剂中的利福平     

章安源  张志民  苏梅  张传津  冯涛  陈玲  刘道中 《中国兽药杂志》2018,52(5):42-48

为建立麻黄鱼腥草散、鸡痢灵散、定喘散、银翘散、金花平喘散、四黄止痢颗粒6种中兽药散剂中非法添加利福平高效液相色谱检查方法,研究使用C18(4.6 mm×250 mm,5μm)色谱柱,样品经前处理后用流动相稀释(乙腈∶甲醇∶磷酸盐缓冲液=35∶35∶30),采用二极管阵列检测器(HPLC-DAD),波长采集范围为200~400 nm,记录色谱图波长为237 nm,流速为1.0 mL/min,进样量为10μL。结果表明,利福平进样浓度在5~250μg/mL范围内与峰面积呈良好的线性关系(r2=0.9997),平均回收率在92.9%~93.7%之间,RSD在0.4%~1.13%之间(n=6)。试验方法简便、准确、可行,适用于兽用中药制剂中非法添加利福平的定性定量检测。  相似文献   

代谢组学在中兽药领域的发展解析     

董玲玲  汪霞 《中国兽药杂志》2018,52(4):73-77

代谢组学作为系统生物学的一部分,其整体性的研究思路与中医药整体观念相一致。介绍了代谢组学的概念和研究方法,根据其在中药领域的应用现状预测其在中兽药作用机制研究、毒理及安全评价研究、质量控制方面具有良好的应用前景,同时对其在未来发展中面临的挑战进行了探讨。  相似文献   

Equine chlamydiosis—An emerging infectious disease requiring a one health surveillance approach          下载免费PDF全文

K. A. Taylor  D. Durrheim  J. Heller  B. O'Rourke  K. Hope  T. Merritt  P. Freeman  C. Chicken  J. Carrick  J. Branley  P. Massey 《Zoonoses and public health》2018,65(1):218-221

Psittacosis is a rare but potentially fatal zoonosis caused by Chlamydia psittaci, an organism that is typically associated with bird contact. However C. psittaci is capable of infecting other non‐avian hosts, such as horses, sheep, cattle and goats. Stud staff and veterinarians have significant exposure to parturient animals and reproductive materials in their routine work. To investigate the zoonotic potential associated with the emergence of C. psittaci as an abortifacient agent in horses, we established a programme of joint human and animal surveillance in a sentinel horse‐breeding region in Australia. This programme comprised cross‐notification of equine cases to public health agencies, and active follow‐up of known human contacts, including stud workers, foaling staff, veterinarians and laboratory staff. We identified no confirmed cases of acute psittacosis despite intensive surveillance and testing of heavily exposed contacts; however, further work in the area is needed.  相似文献