全文获取类型
收费全文 | 17101篇 |
免费 | 718篇 |
国内免费 | 1802篇 |
专业分类
林业 | 676篇 |
农学 | 2020篇 |
基础科学 | 1008篇 |
2045篇 | |
综合类 | 7622篇 |
农作物 | 1865篇 |
水产渔业 | 546篇 |
畜牧兽医 | 1640篇 |
园艺 | 888篇 |
植物保护 | 1311篇 |
出版年
2024年 | 82篇 |
2023年 | 285篇 |
2022年 | 468篇 |
2021年 | 591篇 |
2020年 | 654篇 |
2019年 | 792篇 |
2018年 | 631篇 |
2017年 | 748篇 |
2016年 | 941篇 |
2015年 | 759篇 |
2014年 | 926篇 |
2013年 | 980篇 |
2012年 | 1209篇 |
2011年 | 1159篇 |
2010年 | 999篇 |
2009年 | 903篇 |
2008年 | 791篇 |
2007年 | 734篇 |
2006年 | 678篇 |
2005年 | 512篇 |
2004年 | 423篇 |
2003年 | 364篇 |
2002年 | 306篇 |
2001年 | 302篇 |
2000年 | 282篇 |
1999年 | 262篇 |
1998年 | 228篇 |
1997年 | 303篇 |
1996年 | 276篇 |
1995年 | 260篇 |
1994年 | 260篇 |
1993年 | 256篇 |
1992年 | 313篇 |
1991年 | 300篇 |
1990年 | 246篇 |
1989年 | 112篇 |
1988年 | 66篇 |
1987年 | 42篇 |
1986年 | 21篇 |
1985年 | 19篇 |
1984年 | 17篇 |
1983年 | 7篇 |
1982年 | 20篇 |
1981年 | 16篇 |
1980年 | 9篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 18篇 |
1962年 | 12篇 |
1955年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 359 毫秒
81.
82.
AIM:To explore the changes of L-selectin expression on peripheral blood polymorphonuclear leukocytes (PMNs) and their significance in rats with acute lung injury (ALI). METHODS:ALI model in rat was established by intravonous injection of E. coli endotoxin (ET). The expression of L-selectin on peripheral blood PMNs was measured by immunofluorescence and flow cytometry.RESULTS:The contribution of L-selectin fluorescence signal was on the surface of PMNs membrane. The expression of L-selectin on poeripheral blood PMN was significantly lower at 5 min after injection of ET and the lowest during 15 min to 30 min, then gradually increased, but the expression of L-selectin on PMN was lower at 60 min after injection of ET than that of control animal.CONCLUSION:In physiological state, L-selectin were expressed on the surface of PMN membrane. The protein expression of L-selectin on PMNs reduced rapidly after injection of ET and the lowest at 15 min, then gradually increased. L-selectin may play a role in the development of ALI. 相似文献
83.
Detection of Colletotrichum coccodes and Helminthosporium solani in soils by bioassay 总被引:1,自引:0,他引:1
The sensitivity of a bioassay in detecting soil inoculum of Colletotrichum coccodes and Helminthosporium solani was examined using potato minitubers and microplants. Tests were conducted on soils which were collected from fields in which the interval after a previous potato crop differed, and which were also artificially infested with conidia or microsclerotia. For C. coccodes , determining plant infection based on the occurrence of infected roots after 9–12 weeks was a sensitive method for detecting and quantifying the amount of inoculum in soil. Infestations of less than 0·4 microsclerotia per g soil were detected in artificially infested soils. A semiselective medium, developed for isolating C. gloeosporioides from pepper, detected soil infestations by C. coccodes as low as nine conidia or one microsclerotium per g soil in artificially infested soil. For H. solani , infection on minitubers was a sensitive measure, with soil inoculum of fewer than 10 conidia per g soil being detected. Soil infestation could be quantified by assessing the percentage surface area of minitubers covered by sporulating lesions, which was strongly related to the amount of soil infestation. The results of these bioassay tests were compared with published results for real-time quantitative PCR assays on the same soils. The two methods were in good agreement in artificially infested soils, but the bioassay appeared to be more sensitive with naturally infested soils. 相似文献
84.
Y. J. Huang C. Toscano-Underwood B. D. L. Fitt † X. J. Hu A. M. Hall 《Plant pathology》2003,52(2):245-255
Ascospores of both A-group and B-group Leptosphaeria maculans germinated at temperatures from 5 to 20°C on leaves of oilseed rape. Germination of ascospores of both groups started 2 h after inoculation and percentage germination reached its maximum about 14 h after inoculation at all temperatures. Both the percentage of A-/B-group ascospores that had germinated after 24 h incubation and germ tube length increased with increasing temperature from 5 to 20°C. Germ tubes from B-group ascospores were longer than those from A-group ascospores at all temperatures, with the greatest difference at 20°C. Hyphae from ascospores of both groups penetrated the leaves predominantly through stomata, at temperatures from 5 to 20°C. A-group ascospores produced highly branched hyphae that grew tortuously, whereas B-group ascospores produced long, straight hyphae. The percentage of germinated ascospores that penetrated stomata increased with increasing temperature from 5 to 20°C and was greater for A-group than for B-group L. maculans after 40 h incubation. 相似文献
85.
86.
Genetic variation among asexual progeny of Phytophthora infestans detected with RAPD and AFLP markers 总被引:6,自引:1,他引:6
Genotypic variation among 32 single-zoospore isolates (SZI) of Phytophthora infestans , derived asexually from two hyphal-tip parental isolates (PI-105 and PI-1) of the US-8 genotype, was assessed with 80 random amplified polymorphic DNA (RAPD) primers and 18 amplified fragment length polymorphic DNA (AFLP) primer pairs. In previous investigations, the SZIs from parental isolate PI-105 showed high levels of virulence variability and were differentiated into 14 races, whereas the SZIs from PI-1 showed identical virulence to the parent. The purpose of this investigation was to determine if phenotypic variation observed among SZIs of P. infestans could be detected at the DNA level in these isolates. Polymorphism was detected with 51 RAPD primers and with all 18 AFLP primer pairs in PI-105 SZIs. In SZIs from PI-1, polymorphism was also detected with 25 RAPD primers and 17 AFLP primer pairs. Cluster analysis using the unweighted pair-group method with arithmetic averages (UPGMA) separated the SZIs from parent PI-105 into six virulence groups, 11 RAPD groups and three AFLP groups. Cluster analysis of PI-1 SZIs, which all belong to the same virulence group, differentiated them into four RAPD groups and six AFLP groups. No close correlation among RAPD, AFLP and virulence groups could be established within the two progenies of SZIs. Results of this study suggest that there is a considerable level of inherent genetic variability among SZIs derived asexually from the same parental isolate. The possible mechanisms and implications of this genetic variation are discussed. 相似文献
87.
Marlene Cordero Pedro L. Ramos Lester Hernández Ana I. Fernández Ana L. Echemendía Rudy Peral Gloria González Daniel García Surey Valdés Ana Estévez Keren Hernández 《Phytoparasitica》2003,31(5):478-489
The presence of a begomovirus in potato plants with yellow mottle symptoms was determined for the first time in Cuba. The
incidence of typical begomovirus-like symptoms in potato plants in some regions of Havana province (Güira de Melena, San José
de las Lajas, Güines and Boyeros) during the growing seasons from 1992 to 1998 was in general low. However, in some cultivars
belonging to the National Program for Potato Genetic Improvement, the incidence reached 100%. Yield losses, determined in
1992 and 1994, ranged as high as 19% to 56.33% depending on the cultivar. Characterization of the causal agent was done by
light microscopy, host range (graft and mechanical transmission), DNA hybridizations, polymerase chain reaction, and restriction
fragment length polymorphism analysis. Nucleotide sequence of the amplified fragments revealed the presence ofTomato mottle Taino virus. The virus was transmittedvia tubers and has been detected in mixed infections withPotato virus X and withPotato leaf roll virus.
http://www.phytoparasitica.org posting Oct. 20, 2003. The first two authors contributed equally to this work. 相似文献
88.
G. DAHAL 《Integrated Pest Management Reviews》1997,2(3):139-159
Research on insect pests (including leafhoppers and planthoppers) in Nepal was initiated in 1956 by the Entomology Division and Plant Protection Units of the Department of Agriculture. More systematic work began after the establishment of a Rice Improvement Programme in 1972. Because of poor documentation and publication of research findings, it is difficult to assess the research efforts and achievements to date. The available research reports are mostly published in the proceedings of national seminars/workshops. They are used here to assess the current status of research on the occurrence and damage caused, distribution and composition, relative abundance, biology and management through chemicals. Much of past research has focused on insect surveys by light traps and sweep nets and the evaluation of insecticides and, since early 1990, some research on rice green leafhoppers (Nephotettix virescens and Nephotettix nigropictus) has begun to determine their relative abundance and population characteristics in relation to their potential as vectors of rice viruses. The challenges to pest management in general and some suggestions for future research needs are discussed, based on the present standing of agricultural research in the country 相似文献
89.
90.