可可毛色二孢菌侵染后的烟草酵母双杂交cDNA文库构建     

邢启凯  燕继晔  张玮  刘梅  富春元  李兴红 《植物病理学报》2017,47(5):716-720

正葡萄是世界上最重要的经济果树之一,据联合国粮食及农业组织统计,截止到2013年,中国葡萄栽培面积已达715.5万hm2,葡萄总产量超2 300万kg。长期以来,葡萄病害是影响葡萄产业健康发展的重要因素。近年来研究发现由葡萄座腔菌科真菌(Botryosphaeriaceae)引起的葡萄溃疡病(Botryosphaeria dieback)是葡萄上的主要枝干病  相似文献   

肉桂枝枯原因研究     

文新  宋力 《热带作物学报》1995,16(2):98-102

肉桂泡盾盲蝽吸食肉桂枝条的汁液，造成微小伤口，并携带可可毛色二孢菌，使肉桂枝条容易被该菌侵染，造成皮层坏死，引发极严重的枝枯病。在某些林分，其它枝梢害虫如肉桂双瓣卷蛾、肉桂小峰、肉桂瘿蚊、肉桂木蛾、肉桂天牛，它们的为害也能不同程度地引起肉桂的枝梢枯死。  相似文献   

芒果可可球二孢蒂腐病菌生物学培养特性   总被引：3，自引：1，他引：2  

李敏  胡美姣  岳建军  高兆银  杨凤珍 《热带作物学报》2009,30(11):1660-1664

对芒果可可球二孢蒂腐病菌(Botryodiplodia theobromae Pat.)的生物学培养特性进行了研究.结果表明:菌丝生长最适温度30℃;孢子萌发最适温度30℃;菌丝生长最适pH值5～6,孢子萌发最适pH值7～10;菌丝生长的最佳碳源是棉子糖、葡萄糖,最佳氮源是牛肉浸膏、蛋白胨:光照及振荡培养对菌丝生长无显著影响;菌丝的致死温度为60℃,30 min或65℃,10 min,孢子的致死温度50℃,15min.  相似文献   

高良姜叶枯病病原菌的分离和鉴定     

陈旭玉  甘炳春  冯锦东 《安徽农业科学》2014,(16):5031-5032

[目的]明确高良姜叶枯病病原菌.[方法]病原菌从高良姜感病组织上分离并依据柯赫氏法则进行致病性测定,病原菌的鉴定主要通过形态鉴定和ITS序列分析.[结果]高良姜叶枯病病原菌确定为可可毛色二孢（Lasiodiplodia theobromae）,属子囊菌门（Ascomycota）,座囊菌纲（Dothideomycetes）,葡萄座腔菌目（Botryosphaeriales）,葡萄座腔菌科（Botryosphaeriaceae）,毛色二孢属（Lasiodiplodia）.[结论]首次报道高良姜叶枯病病原菌为可可毛色二孢.  相似文献   

桉树枯枝病病害研究   总被引：1，自引：0，他引：1  

刘月廉 《中国森林病虫》2000,19(1):6-8

按树枯枝病 Botryodiplodia theobromae Pat主要为害 2～ 3龄雷林 1号桉 3 3 ,造成枯枝落叶和死亡。病原菌周年为害 ,以当年 1 2月份至翌年 3月份表现最严重。  相似文献   

粗毛褐孔菌化学成分研究     

杨修镇 《中国兽药杂志》2012,46(12):40-42

从粗毛褐孔菌乙醇提取物中分离鉴定了5个单体化合物,经过分析鉴定,分别为羊毛甾二烯二醇（I）、麦角甾醇（Ⅱ）、齿孔酸（Ⅲ）、D－阿拉伯糖醇（Ⅳ）、十八烷酸（V）。首次从该药材中获得各化合物,为药材的综合开发应用提供了科学依据。  相似文献   

Relationship between incidence and severity of cashew gummosis in semiarid north-eastern Brazil     

J. E. Cardoso†‡  A. A. Santos  A. G. Rossetti  J. C. Vidal 《Plant pathology》2004,53(3):363-367

The incidence–severity relationship for cashew gummosis, caused by Lasiodiplodia theobromae , was studied to determine the feasibility of using disease incidence to estimate indirectly disease severity in order to establish the potential damage caused by this disease in semiarid north-eastern Brazil. Epidemics were monitored in two cashew orchards, from 1995 to 1998 in an experimental field composed of 28 dwarf clones, and from 2000 to 2002 in a commercial orchard of a single clone. The two sites were located 10 km from each other. Logarithmic transformation achieved the best linear adjustment of incidence and severity data as determined by coefficients of determination for place, age and pooled data. A very high correlation between incidence and severity was found in both fields, with different disease pressures, different cashew genotypes, different ages and at several epidemic stages. Thus, the easily assessed gummosis incidence could be used to estimate gummosis severity levels.  相似文献   

Control of postharvest diseases of rambutan using controlled atmosphere storage and potassium metabisulphite or<Emphasis Type="Italic">Trichoderma harzianum</Emphasis>     

D.?SivakumarEmail author  R.?S.?Wilson Wijeratnam  R.?L.?C.?Wijesundera  M.?Abeyesekere 《Phytoparasitica》2002,30(4):403-409

Botryodiplodia theobromae, Colletotrichum gloeosporioides andGliocephalotrichum microchlamydosporum are the causal fungi of three rambutan postharvest diseases, stemend rot, anthracnose and brown spot, respectively. Two different treatments of rambutan fruits to control the diseases were investigated: application of potassium metabisulphite (250 ppm) or culture filtrate ofTrichoderma harzianum (TrH 40) followed by controlled atmosphere storage (CA) at 13.5°C and 95% r.h. Potassium metabisulphite at 250 ppm under CA effectively controlled the incidence and severity of the three postharvest diseases and maintained the eating quality and color of the fruit for 21 days. The greatest effect of this treatment was on brown spot disease, caused byG. microchlamydosporum. Application of TrH 40 was less effective than potassium metabisuphite. http://www.phytoparasitica.org posting July 8, 2002.  相似文献   

茶树病害病原菌Lasiodiplodia theobromae生物学特性研究     

包兴涛  李冬雪  任亚峰  王雪  尹桥秀  王德炉  王勇  武娴  陈卓 《中国植保导刊》2019,(4):5-12

采用皿内测定方法对贵州省惠水县茶树叶斑病的病原菌Lasiodiplodia theobromae进行了病原生物特性研究。结果表明,其病原菌可在SM、PDA、OA和MEA培养基上生长,菌株在MEA培养基上的生长速率显著快于SM培养基、PDA培养基和OA培养基。病原菌适宜在OA培养基上产孢。碳素和氮素营养可影响菌株的生长及菌丝色素的形成。在PDA培养基上,菌株在33℃条件下的生长速度最快,可适应酸性和碱性条件。但在弱碱条件下生长最为迅速。菌株可在PDB培养基中生长,接种菌碟后生长88 h进入稳定期。  相似文献   

可可毛色二孢β-1,4-葡聚糖内切酶LtEg1信号肽的鉴定及其酶活性分析     

彭军波  李兴红  张玮  刘梅  邢启凯  燕继晔 《植物保护学报》2020,47(1):119-126

为明确可可毛色二孢β-1,4-葡聚糖内切酶LtEg1是否能够分泌至胞外及其活性,采用酵母互补试验分析了内切酶LtEg1的信号肽活性,利用3,5-二硝基水杨酸法检测了内切酶LtEg1活性,通过实时荧光定量PCR测定了LtEg1基因在不同侵染阶段的表达量。内切酶LtEg1的信号肽和酵母蔗糖酶的融合表达能够引起酵母蔗糖酶的外泌,使得酵母转化子能够在以棉子糖为唯一碳源的培养基上生长。内切酶LtEg1能够以羧甲基纤维素钠为底物,发挥其酶活功能。与营养菌丝阶段相比,LtEg1基因在病原菌侵染阶段的表达水平显著升高;且在接种后48 h,LtEg1基因的表达量达到高峰,约为营养菌丝阶段的12倍,表明内切酶LtEg1作为重要的外泌蛋白酶,在可可毛色二孢侵染致病过程发挥了重要作用。  相似文献