全文获取类型
收费全文 | 7069篇 |
免费 | 328篇 |
国内免费 | 755篇 |
专业分类
林业 | 413篇 |
农学 | 1407篇 |
基础科学 | 89篇 |
821篇 | |
综合类 | 2764篇 |
农作物 | 1049篇 |
水产渔业 | 179篇 |
畜牧兽医 | 696篇 |
园艺 | 431篇 |
植物保护 | 303篇 |
出版年
2024年 | 33篇 |
2023年 | 105篇 |
2022年 | 218篇 |
2021年 | 196篇 |
2020年 | 269篇 |
2019年 | 266篇 |
2018年 | 210篇 |
2017年 | 326篇 |
2016年 | 337篇 |
2015年 | 305篇 |
2014年 | 373篇 |
2013年 | 513篇 |
2012年 | 507篇 |
2011年 | 549篇 |
2010年 | 487篇 |
2009年 | 430篇 |
2008年 | 426篇 |
2007年 | 460篇 |
2006年 | 306篇 |
2005年 | 309篇 |
2004年 | 209篇 |
2003年 | 173篇 |
2002年 | 120篇 |
2001年 | 107篇 |
2000年 | 103篇 |
1999年 | 132篇 |
1998年 | 98篇 |
1997年 | 94篇 |
1996年 | 79篇 |
1995年 | 52篇 |
1994年 | 68篇 |
1993年 | 58篇 |
1992年 | 56篇 |
1991年 | 40篇 |
1990年 | 36篇 |
1989年 | 28篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 11篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1955年 | 2篇 |
排序方式: 共有8152条查询结果,搜索用时 15 毫秒
81.
To facilitate weed suppression, oilseed rape or canola (Brassica napus) has been genetically modified (GM) over the last two decades to incorporate herbicide tolerance (HT). The introduction of oilseed rape in commercial agriculture raises concerns about potential adverse agronomic and environmental effects linked to both the genetic modification and altered agricultural practice. How will this new crop modify the existing cropping practice? What are the potential agro-environmental implications of its introduction? This paper analyses and schematises recorded and conceivable agricultural practice changes with GMHT oilseed rape introduction and its likely agro-environmental effects. It develops a conceptual systematics of influencing factors, subsequent practice changes and likely agro-environmental effects. The results can be used to design the mandatory monitoring of adverse GM crop effects. 相似文献
82.
《Communications in Soil Science and Plant Analysis》2012,43(1):63-69
Abstract The absorption mechanisms for Na, K, SO4 and Cl were tested in a salt tolerant (PVR 1) and a salt sensitive (GEB 24) rice varieties. The salt tolerant variety accumulated significantly larger amounts of Na than the salt sensitive variety. Further, PVR 1 absorbed SO4 from Na2SO4 in preference to that from K2SO4. The absorption patterns for K and Cl were similar in both the varieties. It is concluded that the capacity of plant species to accumulate greater amounts of Na is a reflection of their halophytic feature. 相似文献
83.
生物炭表面水溶活性分子可以有效提高水稻的耐旱性 总被引:1,自引:0,他引:1
生物炭是一种可以改良土壤、增强作物产量和提升作物品质的新型农林废弃物再利用材料。本研究通过振荡方式制备生物炭浸提液,利用水培系统培养水稻幼苗,以20%PEG6000、300 mmol/L和500 mmol/L甘露醇模拟干旱胁迫,研究生物炭表面水溶活性分子对水稻幼苗抗旱性的影响。研究结果发现:生物炭浸提液可有效缓解干旱胁迫造成的水稻种子萌发和幼苗生长抑制,缓解叶绿素含量、鲜重及存活率的降低,同时可以降低体内的活性氧积累等。实时定量RT-PCR检测表明生物炭浸提液促进干旱胁迫响应标志基因的表达量。研究结果说明生物炭浸提液可以提高干旱胁迫下水稻幼苗的抗氧化能力,进一步提高水稻幼苗对干旱胁迫的耐受性。 相似文献
84.
L. Song D.‐W. Zhang F.‐M. Li X.‐W. Fan Q. Ma N. C. Turner 《Journal of Agronomy and Crop Science》2010,196(5):323-335
Competition for water generates a classic aspect of the tragedy of the commons, the ‘race for fish’, where crops must allocate more resource to acquisition of the limiting resource than is optimal for crop yield allocation. A pot experiment using a simple additive (target–neighbour) design was conducted to examine the above‐ground and below‐ground growth of three spring wheat (Triticum aestivum L.) cultivars when grown alone and in mixtures at three levels of water availability. The effects of competition and water availability were compared by observing patterns of growth, biomass allocation and below‐ground outcomes. Competitive interactions were investigated among cultivars ‘HST’, ‘GY602’ and ‘LC8275’, target plant of each cultivar grown without neighbouring plants are referred to herein as control plant and one target plant of each cultivar sown surrounded either by same or another cultivar as intra‐ or inter‐cultivar competition. Competitive ability was assessed as the response ratio (lnRR) between the target plant surrounded by six other plants and the target plant in isolation. Our results showed that the cultivar ‘HST’, released over a century ago, produced a higher biomass and grain yield than the more recently released cultivars ‘LC8275’ and ‘GY602’ when grown as isolated plants with sufficient water supply. However, competition for resources from neighbours led to target plant biomass and grain yield being significantly reduced relative to controls in all three cultivars, particularly in ‘HST’. When subjected to intra‐cultivar competition, the two recently released cultivars ‘LC8275’ and ‘GY602’ had higher grain yields and water use efficiency for grain than ‘HST’ in all three water regimes. The landrace ‘HST’ had better and significantly linear relationships between biomass and biomass allocation, root length and specific root length, whereas the recent and modern cultivars had much more water‐related species‐specific changes in root morphology and allocation patterns. These results suggest that crop traits that influence competitive ability, such as biomass allocation to roots and root plasticity in response to drought have changed in modern wheat cultivars because of breeding and selection. 相似文献
85.
86.
Studies of biological responses in the terrestrial environment to rapid changes in climate have mostly been concerned with aboveground biota, whereas less is known of belowground organisms. The present study focuses on mites and springtails of heathland ecosystems and how the microarthropod community has responded to simulated climate change in a long-term field experiment. Increased temperature and repeated drought was applied for 13 years to field plots located in Wales, The Netherlands and Denmark representing sites of contrasting climatic conditions with respect to precipitation and temperature. This approach provided an opportunity to study biological responses on a local (within sites) and regional scale. Warming treatments increasing night time temperature (0.3–1 °C higher than ambient at 5 cm soil depth) had no detectable effects on the microarthropod communities. Increased intensity and frequency of drought had only weak persistent effects on springtail species composition, but practically no effect on major mite groups (Oribatida, Prostigmata or Mesostigmata) suggesting that ecosystem functions of microarthropods may only be transiently impacted by repeated spring or summer drought. 相似文献
87.
新牧一号苜蓿MvP5CS基因的克隆和功能分析 总被引:1,自引:0,他引:1
为研究苜蓿(Medicago sativa)耐盐分子机制,为抗逆分子育种提供依据,通过同源克隆和RT PCR技术克隆了新牧一号杂花苜蓿(M.varia Xinmu 1)的MvP5CS基因, MvP5CS全长2 148 bp,Genbank登录号为:EU371644。荧光定量PCR分析发现,在盐胁迫下MvP5CS基因表达明显上调,说明 MvP5CS与苜蓿的耐盐性相关。利用农杆菌介导的叶盘转化法,将MvP5CS基因成功转入烟草(Nicotiana tabacum),盐胁迫下转MvP5CS基因T1代烟草萌发率和根长均高于非转基因烟草。表明新牧一号苜蓿P5CS基因能够提高转基因烟草的耐盐性。 相似文献
88.
应用生物信息学方法在GenBank数据库查找到1条编码大豆LEA3蛋白的高赖氨酸蛋白基因PM8(登录号:Z22872),并利用RT-PCR方法从大豆干种子中克隆得到该基因,序列分析表明,PM8基因与GenBank上的序列存在5个碱基的差异,使编码蛋白的赖氨酸重量比由原来的19.65%增加为19.93%。构建了PM8基因的植物表达载体pEMT-PM8,用农杆菌介导法转化烟草。从转PM8基因的PCR阳性植株中选取5个既抗盐又抗旱的株系进行Real-timePCR检测。结果表明,PM8基因全部超量表达。研究为培育高营养品质且抗逆的转基因作物研究提供了一条新的途径,具有重要的研究价值。 相似文献
89.
[目的]分析盐藻DNA对水稻幼苗在低盐中生长的影响。[方法]将刚萌发的水稻幼苗根系浸入5 mg/L盐藻DNA溶液中培养2d,以蒸馏水培养为对照,处理后的水稻幼苗栽于含有氯化钠3 g/L的MS培养液中培养,分析盐藻DNA对水稻幼苗在低盐中生长的影响。[结果]水稻幼苗用5 mg/L盐藻DNA处理后,在含氯化钠3 g/L的MS培养液中培养15 d,其平均株重为150.7 mg,比对照增加了10.3%;平均株高为15.28 cm,比对照增加了6.1%;平均存活率为45.0%,比对照增加了125.0%;平均根数为7.15条,比对照减少了2.8%;平均根长为3.83 cm,比对照缩短了8.4%。说明盐藻DNA提高了水稻幼苗的耐盐适应性。[结论]盐藻DNA可提高水稻的耐盐适应性。 相似文献
90.