Estimation of genetic parameters,provenance performances,and genotype by environment interactions for growth and stiffness in lodgepole pine (Pinus contorta)     

Haleh Hayatgheibi  Anders Fries  Johan Kroon 《Scandinavian Journal of Forest Research》2019,34(1):1-11

  相似文献   

Numerical classification of related Peruvian highland maize races using internal ear traits     

Rodomiro Ortiz  Ricardo Sevilla  Gregorio Alvarado  José Crossa 《Genetic Resources and Crop Evolution》2008,55(7):1055-1064

  相似文献   

Longitudinal identification of Enterocytozoon bieneusi in dairy calves on a farm in Southern Xinjiang,China     

《Comparative immunology, microbiology and infectious diseases》2020

Enterocytozoon bieneusi is the most common species responsible for human and animals microsporidiasis. A total of 250 samples were collected weekly from 25 newborn dairy calves of a farm in Southern Xinjiang, China at one to ten weeks of age. Enterocytozoon bieneusi was identified and genotyped by nested PCR amplification and sequencing of internal transcribed spacer (ITS) region.The cumulative prevalence of E. bieneusi infection was 100% (25/25), and the average infection was 52.0% (130/250). The highest infection rate was recorded at six weeks of age (92.0%, 23/25), and no infection was observed at one and two weeks of age. Sequencing analysis showed nine E. bieneusi genotypes (J, EbpC, PigEBITS5, CHV4, CHC3, CS-9, KIN-1, CH5, and CAM5) were identified. The highest genetic polymorphism was observed at ten weeks of age. Genotype J was the predominant E. bieneusi genotype. Phylogenetic analysis clustered genotype J into Group 2 and other eight genotypes (EbpC, PigEBITS5, CHV4, CHC3, CS-9, KIN-1, CH5, and CAM5), detected in 22 (16.9%, 22/130) samples, into Group 1. Among the genotypes, EbpC, KIN-1, and J have been identified in humans. The highest E. bieneusi infection rate (57.9%, 124/214) was observed in fecal samples with formed feces with no diarrhea (p < 0.01), and high genetic polymorphism was observed in class I fecal samples. The presence of zoonotic E. bieneusi genotypes in dairy calves suggests the possibility of transmitting zoonotic infections to humans. It provides the basic data on dynamic change of E. bieneusi in calves.  相似文献   

G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015     

《Comparative immunology, microbiology and infectious diseases》2019

The aim of this retrospective study was to use RT-PCR and nucleotide sequencing analysis to determine the G (VP7 gene) and P (VP4 gene) genotypes of 155 Brazilian bovine rotavirus A (RVA) wild-type strains detected in diarrheic calves from all Brazilian geographical regions from 2006 to 2015. The RVA strains evaluated belonged to the G6, G10, P[5], and P[11] genotypes. The G6P[5] genotype was prevalent (65.5%; P < 0.05) in beef, and the G10P[11] (38.4%) and G6P[11] (30.8%) genotypes were more prevalent in dairy cattle herds. The Midwest was the region with the highest number of genotyped RVA strains, where the genotypes G6, P[5], and P[11] were identified. Genotype combination G6-IV/P[5]-IX, prevalent in beef herds, and G6-III/P[11]-III or G10-IV/P[11]-III, prevalent in dairy herds, were detected. In addition, for the first time in Brazil, we detected the P[5] and P[11] genotype RVA strains that belong to lineage II and VII, respectively.  相似文献   

Molecular characterization and phylogenetic analysis of new Newcastle disease virus isolates from the mainland of China   总被引：2，自引：0，他引：2  

Liu H  Wang Z  Wu Y  Wu Y  Sun C  Zheng D  Xu T  Li J 《Research in veterinary science》2008,85(3):612-616

Seventy-nine velogenic Newcastle disease virus (NDV) isolates were obtained from infected chicken flocks during the outbreaks of Newcastle disease (ND) in various regions of the mainland of China in 2006. The F gene fragment (535 bp, from nt 47 to 581 of the F gene) which codes the main functional region of the F protein was obtained by RT-PCR and sequenced. All sequences obtained in this study have been submitted to GenBank. All the isolates have the motif ¹¹²R-R-Q/R-K/R-R-F¹¹⁷ at the cleavage site of the fusion protein, which is typical of velogenic NDV isolates. For genotyping, a phylogenetic tree based on nucleotides 47–435 of the F gene was constructed, and the 79 isolates could be divided into two genotypes, namely VIId and III. Most of the isolates proved to be of genotype VIId; only two isolates were of genotype III. Genotype VIId NDV has been the predominant pathogen responsible for most Newcastle disease outbreaks in China. The proportion of isolates of genotype VIId NDV shows an increasing trend, according to studies on the molecular epidemiology of NDV in China from 2002 to 2006.  相似文献   

西瓜离体再生高效基因型材料的筛选   总被引：1，自引：0，他引：1  

孙治图  许勇  张海英  李名扬 《中国瓜菜》2008,21(3):5-9

为完善西瓜离体再生体系,以23种基因型材料的西瓜无菌苗子叶为试材,运用方差分析筛选高效基因型材料及芽诱导最适培养基。结果表明:基因型不同的材料,诱导效果有明显差异。筛选的再生高效基因型为鲁圆、三白、FW351、PM2和京母。鲁圆诱导效果最好,最高诱导率达到90.0%,与18种基因型材料的诱导差异达到了显著水平;三白、FW351、PM2和京母的诱导效果次之,诱导率分别为85.0%、82.5%、82.5%和80.0%。筛选的芽诱导最适培养基为MS+6-BA3.0mg/L。  相似文献   

棉花三种杂种色素腺体性状的研究     

万黎明  彭锁堂 《山西农业大学学报(自然科学版)》1998,18(2):102-104

对以（亚洲棉×比克氏棉）Ｆ１异源四倍体为母本,用海岛棉及陆地棉有色素腺体和无色素腺体不同类型做父本,杂交合成的种间三交种子叶色素腺体延缓发生性状进行了比较研究,结果表明：不同杂交组合在各不同组织器官上对比克氏棉子叶腺体延缓发生性状有明显差异,Ｇｌ２Ｇｌ２Ｇｌ３Ｇｌ３所产生的在三交种种子上有少量腺体,但包括花器在内的其它器官和组织均为有色素腺体类型;ｇｌ２ｇｌ２ｇｌ３ｇｌ３所产生的三交种种子和花器均无色素腺体,而植株也为少色素腺体类型。  相似文献   

晚粳稻籽粒中As、Cd、Cr、Ni、Pb等重金属含量的基因型与环境效应及其稳定性   总被引：13，自引：0，他引：13  

程旺大  张国平  姚海根  吴伟  汤美玲  朱祝军  徐民 《作物学报》2006,32(4):573-579

选择籽粒Cd、Cr、As、Ni和Pb等重金属含量差异较大的12个晚粳稻基因型，种植于浙江省晚粳稻主产区嘉兴、湖州、杭州、宁波、绍兴等市的6个试点，研究籽粒中5种重金属含量的基因型与环境变异及其稳定性、籽粒和土壤有效态（DTPA提取态）Cd、Cr、As、Ni和Pb等重金属含量之间及与Fe、Zn含量之间的关系，以及土壤pH对籽粒重金属积累的影响。结果表明，环境、基因型及其互作效应对籽粒重金属含量的效应均达极显著水平，表明筛选和选育籽粒重金属含量低的品种以及通过农艺措施减少籽粒重金属含量是可能的。同时，籽粒中这5种重金属含量的稳定性因环境、基因型而有较大差异，且亦因重金属种类而异。因此，为降低籽粒重金属含量，应针对特定重金属污染的环境进行基因型选择，并同时考虑基因型籽粒重金属含量的稳定性。土壤pH不仅影响土壤重金属有效性及水稻籽粒中重金属积累量，还影响籽粒重金属积累的基因型与环境互作效应（即积累稳定性）。此外，土壤中一些重金属常发生复合污染，如Cd和Cr、As或Ni，Cr和As，Pb和As间表现为协同消长，Cd含量较高的稻米往往As和Pb含量也高，Cr和Ni含量以及As和Pb含量之间也呈正相关。  相似文献   

不同基因型玉米氮素吸收利用效率研究进展   总被引：1，自引：0，他引：1  

申丽霞  王璞 《玉米科学》2016,24(1):50-55

玉米氮效率的评价方法与指标不尽一致,导致对玉米基因型不同的分类结果。玉米氮效率研究主要集中于根系对氮素的吸收、叶片对氮素的还原同化、氮素在植株体内的积累和分配以及地上部生长势与氮效率的关系方面。以子粒为主要收获对象的玉米,穗、子粒发育状况对玉米产量起着关键作用,有关穗、子粒发育对玉米氮效率贡献率的大小、不同氮效率基因型玉米穗、子粒发育的差异以及内在生理生化机制研究较少。对不同氮效率基因型玉米子粒发育与氮效率之间关系、子粒发育尤其是顶部子粒的早期发育(决定穗粒数的形成)对氮素的反应、氮高效品种顶部子粒的早期发育是否优于氮低效型、氮素对不同氮效率基因型玉米子粒发育的影响机制等问题有待进一步研究。  相似文献   

水牛乳β-酪蛋白基因多态性与消化性能及抗氧化性能的关联研究     

赵烜影  郭鸰  任大喜  李楠  李玲  刘振民 《中国奶牛》2022,(1):12-16

本研究以水牛乳β-酪蛋白(β-CN)基因多态性分析结果为依据,从水牛乳中分离并纯化出具有活性的β-酪蛋白亚型.采用体外消化模型分析其在胃肠道中的消化性能;根据水解液的抗氧化活性来分析生物活性肽的释放.高效液相色谱法(HPLC)结果显示,水牛乳中β-CN有A和B两种等位基因.消化性能结果显示A等位基因更利于β-酪蛋白在肠...  相似文献