利用水葫芦象甲和农达综合控制水葫芦   总被引：17，自引：0，他引：17  

丁建清  王韧  付卫东  陈志群 《植物保护》1999,25(4):4-7

试验表明同时应用水葫芦象甲和农达控制水葫芦,可达到快速、持续的控制效果,但农达使用剂量应选择适当。药量４.５ｋｇ／ｈｍ^２的综防处理区中,由于农达的作用水葫芦植株在２０～５０ｄ内大部分死亡,水葫芦象甲亦很快死亡,象甲很难发挥有效的作用;药量为０.４５ｋｇ／ｈｍ^２的综防区中,水葫芦象甲和农达同时作用,对水葫芦的叶片数、繁殖量和生物量起到了明显的抑制作用,与单独施用同样药量的化防区和只释放象甲的生防区有显著差异,而且象甲保持了一定的种群密度;而药量为０.０４５ｋｇ／ｈｍ^２的综防区与只放象甲的生防区控制水葫芦的效果一样。  相似文献   

Improving hybrid seed production in corn with glyphosate‐mediated male sterility     

Paul C C Feng  Youlin Qi  Tommy Chiu  Martin A Stoecker  Christopher L Schuster  Scott C Johnson  Augustine E Fonseca  Jintai Huang 《Pest management science》2014,70(2):212-218

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南繁条件下转基因大豆对根际土壤可培养微生物的影响     

杜伟  黄启星  左娇  罗丰  王旭初  郭安平 《热带作物学报》2012,33(3):417-421

以常规大豆品种中黄13为对照,研究了抗草甘膦转基因大豆AG5601对根际土壤可培养微生物的数量影响。经过大豆2个生长周期的研究发现：在相同时段内,转基因大豆AG5601和对照对根际土壤细菌、真菌和放线菌的数量变化影响趋势基本一致;除在第2生长周期的第30～100天（20100618～20100908）时转基因大豆极显著地抑制了根际土壤真菌数量（p<0.01）外,转基因大豆对根际土壤微生物数量的影响呈不规律变化;转基因大豆对根际土壤微生物有一定的影响,但无明显规律可循。此研究结果为在我国南繁地区开展转基因大豆AG5601安全评价提供数据支持。  相似文献   

抗草甘膦转基因大豆及加工品LAMP检测研究   总被引：9，自引：0，他引：9  

刘彩霞  梁成珠  徐彪  高宏伟  林超  孙敏 《大豆科学》2009,28(2)

将环介导的等温核酸扩增技术应用于转基因大豆及加工品检测.针对抗草甘膦Roundup Ready转基因大豆及加工品外源基因EPSPS设计2对特异性引物进行扩增,成功建立起定性检测转基因大豆及加工品的LAMP检测方法.优化LAMP反应条件,反应温度为65℃,反应时间为1h.结果表明:该体系能快速、灵敏、有效地检测转基因大豆及加工品中整合的EPSPS基因,检测限为0.01%,低于国际现行最低检测量0.5%的要求.检测EPSPS基因操作简单,成本低、特异性强、灵敏度高.LAMP检测结果可信,稳定性好,可对目前批准的抗草甘膦RoundupReady转基因大豆及加工品进行定性检测.  相似文献   

Distribution of [C]glyphosate in mature glyphosate-resistant cotton from application to a single leaf or over-the-top spray     

Paul C.C. Feng  Tommy Chiu 《Pesticide biochemistry and physiology》2005,82(1):36-45

Distribution of [¹⁴C]glyphosate was examined in mature glyphosate-resistant cotton plants at the 13-node stage in the absence of phytotoxicity. Initial experiments employed manual application of glyphosate to individual leaves within a relatively immature (Node 9) or mature (Node 5) sympodium (i.e., fruiting node) in the plant. We measured glyphosate export out of the treated leaf to the fruiting structures and foliage in the sympodium as well as out of the sympodium into the plant. Application to the Stem leaf, Leaf 1 or Leaf 2 in Node 9 showed 30-37% glyphosate export by 14 days after treatment. While Stem leaf exported mainly to the plant, Leaves 1 and 2 exported equally between the plant and the sympodium. Within the sympodium, glyphosate was distributed almost entirely to the fruiting structures with Boll 1 containing the highest level irrespective of the treated leaf. In Node 5, application to the Stem leaf or Leaves 1-3 showed overall lower glyphosate export (20-27%) with Stem leaf and Leaf 3 exporting mainly to the plant, and Leaves 1 and 2 exporting mainly to the sympodium. Within Node 5, the subtending boll of the treated leaf was not the main distribution target, but instead the boll at the next higher sympodial position relative to the treated leaf. Subsequent studies were conducted using over-the-top spray application of [¹⁴C]glyphosate at field use-rates in mature plants at the 13-node stage. Analysis of open bolls at full maturity showed high residues in seeds and fiber of Bolls 1 and 2 in the mature sympodia. Our results suggest that glyphosate distribution in mature plants was affected by sympodial age, position of the treated leaf, and position and sink strength of the bolls.  相似文献