基于白木香ESTs的沉香属SSR分子标记引物筛选     

杨云  张争  陈波等 《江西农业学报》2014,(5):76-79

通过对白木香样品的454GS FLX Titanium高通量测序,获取了大量白木香EST序列,从中搜索出956个SSR,根据引物设计标准,设计了44对EST-SSR引物。在合适的PCR反应体系下,以海南白木香样品的DNA为模板,对设计的EST-SSR引物进行筛选,发现有22对EST-SSR引物能较好地扩增出产物。在沉香属3个种9份样品中进一步对这些可扩增的引物进行多态性初步验证,所筛选引物均可在所有沉香属样品中使用,为后续白木香居群及沉香属植物遗传多样性分析奠定基础。  相似文献   

莲子壳粉袋料栽培茶树菇的研究     

胡志斌  李磊  张树  张玉才  谢远泰 《江西农业学报》2014,(3):98-100

以5种莲子壳粉不同配比配方栽培茶树菇,筛选出了莲子壳粉适合袋栽茶树菇的较优配方:莲子壳粉52%、棉籽壳26%、麦麸20%、石灰2%。采用该配方袋栽茶树菇,菌包菌丝生长较快,34 d菌丝长满菌包,出菇周期短,产量高,4批茶树菇每个菌包平均产量达到341.5 g干菇,生物转化率达68.30%,优于棉子壳袋栽茶树菇的产量。为莲子壳废弃物的利用开辟了新用途。  相似文献   

Identification of sources of resistance to damping-off and early root/hypocotyl damage from Rhizoctonia solani in common bean (Phaseolus vulgaris L.)     

《Crop Protection》2013

Rhizoctonia solani causes economically important root and hypocotyl diseases in common bean throughout the world. Root health is a vital factor in plant development and root diseases would negatively influence water and nutrient uptake as well as cause direct stand reduction and root rot damage to the crop. An efficient common bean screening method to evaluate damping-off and early root/hypocotyl damage from R. solani was developed and used to identify dry bean lines with levels of resistance to this disease. Two sets of 163 and 111 lines previously evaluated for drought tolerance in Nebraska and Puerto Rico were evaluated for damping-off resistance and early root/hypocotyl damage under greenhouse conditions. Disease severity on plants was identified based on above-ground symptoms, seedling survival and root lesions using a rating scale of 1 (resistant) to 9 (susceptible). In the first set of lines representing commonly grown dry bean cultivars, germplasm and sources of damping-off resistance, the Rhizoctonia mean rating ranged from 1.7 to 3.9; Phaseolus vulgaris lines PI 310668 and PI 533249 had the highest damping-off resistance. In the second set of the best lines from a drought tolerance shuttle breeding program the Rhizoctonia mean rating was between 2.6 and 5.7. The availability of drought tolerant dry bean lines allowed the testing of the hypothesis that there was a correlation between selecting for drought tolerance and R. solani damping-off resistance. No correlation between mean disease rating and drought tolerance was found, but adapted dry bean lines such as NE14-08-176 released as SB-DT1, and NE14-08-225 were identified with moderate damping-off resistance and drought tolerance. Lines with both traits and other attributes will facilitate development of resistant bean cultivars to manage damping-off caused by R. solani.  相似文献   

不同药剂预防白蚁蛀蚀木材的效果测定   总被引：2，自引：0，他引：2  

陈红梅  朱建华  张再福  张华庭  陈国顺 《福建林业科技》2000,27(4):45-48

室内采用河砂木块法研究了 15种药剂对白蚁的防治效果 ,48%毒死蜱、5 %锐劲特、48%毒死蜱 + 10 %安绿宝、2 5 %辛硫·灭扫利、5 %锐劲特 + 76 9%樟脑油、10 %安绿宝等 6种药剂 (或混配剂 )抗白蚁蛀蚀效果显著优于氯丹。野外试验了15种药剂 ,2 2个月后的结果表明 :48%毒死蜱单独使用或与 76 9%松节油等混配使用 ,可以保持木材近 2a仍不受白蚁蛀蚀 ;5 %锐劲特、2 5 %辛硫·灭扫利等药剂单独使用或与巴丹等混配使用 ,其效果也优于 70 %氯丹  相似文献   

产涩柿单宁酶菌株的筛选及鉴定     

栾丽杰  杨永涛 《安徽农业科学》2010,38(20):10499-10501

[目的]获得可产生涩柿单宁酶的微生物,并对其鉴定。[方法]微生物在以涩柿为培养基上自然生长,进行划线分离,获得纯种菌株。将菌株接种于溴酚蓝培养基上,选取变色圈较大的8个菌株,进行COD去除率和单宁耐受力试验,选取COD去除率高、单宁耐受力强的菌株,使用形态学观察法进行菌种鉴定。[结果]获得可产生涩柿单宁酶的菌株,初步鉴定为黑曲霉。[结论]利用该方法,可获得产生涩柿单宁酶的黑曲霉,其COD去除率高、单宁耐受力强,有成为工程菌株的潜力。  相似文献   

贝奥雄性不育灭鼠剂室内药效试验   总被引：4，自引：0，他引：4  

尤德康  董晓波  宋玉双  李继成  孙永吉  蒋永利 《中国森林病虫》2006,25(2):32-34

贝奥雄性不育灭鼠剂是一种新型的植物源灭鼠剂,来源于天然植物雷公藤。本试验通过在室内用该药剂喂饲金黄地鼠,检验其对雄鼠的不育作用,结果表明该植物源药剂对雄性金黄地鼠具有显著的不育效果。  相似文献   

不同基因型油菜锌效率机理Ⅰ ——耐低锌油菜基因型苗期筛选指标研究     

徐卫红  王正银  邓晓瑜 《中国农学通报》2006,22(11):164-164

采用营养液培养试验比较了低锌营养下芥菜、白菜、甘蓝型3个油菜(Brassica napus L.)品种幼苗的生物量，抗氧化酶活性，根系活力，叶绿素含量及对锌的吸收、利用差异。试验结果表明，3个油菜品种由于基因型不同，在耐低锌营养能力方面存在显著性差异。在低锌时(10-6mol/L Zn2+)，幼苗植株地上部干重以芥菜型>甘蓝型>白菜型，地上部干重分别较无锌对照处理增加17.8%(芥菜型)、9.9%(白菜型)、3.3%(甘蓝型)。芥菜型叶CAT、POD、SOD活性，叶绿素含量及根系活力也明显高于甘蓝型和白菜型油菜。锌积累量及锌利用率也以芥菜型远远大于其它2个品种，锌素利用效率分别为12.3%（芥菜型），8.0%(白菜型)，4.9% (甘蓝型)。苗期油菜叶CAT、POD、SOD等抗氧化酶活性、叶绿素含量及根系活力可以作为筛选耐低锌营养油菜基因型的评价指标之一。  相似文献   

甜（辣）椒耐低温弱光品种筛选方法初探   总被引：11，自引：0，他引：11  

姜亦巍  胡洽 《华北农学报》1996,11(4):39-42

通过在不同的低温弱光条件下，对８个代表性品种的冷害发生发展规律的研究，初步确定：１５℃／５℃（昼／夜）、光照强度４０００ｌｘ、光照时间８ｈ、处理１５天，是耐低温弱光品种的适宜选择压力。在此条件下，耐低温品种不出现伤害症状和落叶现象，叶片生长速度快；不耐低温品种生长缓慢，落叶严重，且生长点呈黑褐色，以至干枯。品种间差异显著。同时，耐低温品种叶片叶绿素含量增加，而不耐低温品种叶绿素含量下降。这一结果为进一步完善耐低温弱光品种的鉴定方法提供可靠的依据  相似文献   

哈密瓜内生拮抗细菌的筛选及其抑菌物质研究初报     

姜云琴  罗明  张祥林  陈金焕 《中国农学通报》2007,23(3):356-359

通过测定菌体及胞外分泌物抑菌活性,从378个哈密瓜内生细菌中筛选出了对瓜类细菌性叶斑病菌、疫霉病菌具有较强拮抗活性的菌株。对抗菌活性显著的197菌株的胞外抑菌活性物质进行了初步研究。研究显示:197菌株胞外分泌物的抗菌效价可达80倍;其抗菌活性物质为蛋白质类物质,可通过15%-90%饱和度的硫酸铵盐析,其中75%硫酸铵饱和度提取的粗蛋白的抗菌活性最强;该抗菌蛋白具有一定的耐热性,温度高于40℃后抑菌活性逐渐下降,100℃下仍能保持部分抑菌活性,120℃时全部丧失。  相似文献   

The screening toolbox of bioactive substances from natural products: a review     

Wang B  Deng J  Gao Y  Zhu L  He R  Xu Y 《Fitoterapia》2011,82(8):1141-1151

Hunting lead compounds from natural products plays a vital role in finding successful drug candidates. The efficiency of screening campaigns is mainly determined by the validity of selected screening models. To screen desired lead compounds, researchers have developed a plethora of experimental screening models. However, the considerable diversity of screening models from animal models, tissue models, to cell models and so on, may cause some trouble in choosing the suitable one. This review provides a toolbox of experimental screening models that have been used to discover new drug candidates from natural products. Two screening indexes are designed for different research directions in this screening toolbox. Index I is proposed from the direction of screening different objective substance populations, including plant extracts, active fractions and pure compounds; index Π is according to screening different drug properties, including pharmacological properties, pharmacokinetic properties and affinity binding properties. We hope that the abbreviated bibliographies will help readers to quickly retrieve useful information by two screening indexes and provide certain reference value for choosing more appropriate screening models. Finally, we discuss ways of improving model systems, as well as future directions.  相似文献