Calcium Signaling is Involved in Negative Phototropism ofRice Seminal Roots简     

CHEN Juan  岛 Yi-wei  XU Hua-wei 《水稻科学》2014,21(1):39-46

Calcium ions(Ca2+) act as an intracellular second messenger and affect nearly all aspects of cellular life. They are functioned by interacting with polar auxin transport, and the negative phototropism of plant roots is caused by the transport of auxin from the irradiated side to the shaded side of the roots. To clarify the role of calcium signaling in the modulation of rice root negative phototropism, as well as the relationship between polar auxin transport and calcium signaling, calcium signaling reagents were used to treat rice seminal roots which were cultivated in hydroculture and unilaterally illuminated at an intensity of 100–200 μmol/(m2·s) for 24 h. Negative phototropism curvature and growth rate of rice roots were both promoted by exogenous CaCl2 lower than 100 μmol/L, but inhibited by calcium channel blockers(verapamil and LaCl3), calcineurin inhibitor(chlorpromazine, CPZ), and polar auxin transport inhibitor(N-1-naphthylphthalamic acid, NPA). Roots stopped growing and negative phototropism disappeared when the concentrations increased to 100 μmol/L verapamil, 12.500 μmol/L LaCl3, 60 μmol/L CPZ, and 6 μmol/L NPA. Moreover, 100 μmol/L CaCl2 could relieve the inhibition of LaCl3, verapamil and NPA. The enhanced negative phototropism curvature was caused by the transportation of more auxin from the irradiated side to the shaded side in the presence of exogenous Ca2+. Calcium signaling plays a key role as a second messenger in the process of light signal regulation of rice root growth and negative phototropism.  相似文献   

缺磷胁迫下番茄侧根形成与miR164及NAC1表达的关系     

曾后清  朱毅勇  包勇  沈其荣  郭凯  黄思齐  杨志敏 《植物营养与肥料学报》2010,16(1):166-171

为了研究番茄幼苗在缺磷胁迫下根系形态发育与生长素、生长素信号转导途径中的转录因子NAC1,以及调控NAC1 表达的上游miR164之间的关系。试验以5和500 μmol/L磷浓度作为缺磷胁迫和对照,检测了外源生长素NAA（1-naphthalene acetic acid）及生长素抑制剂NPA（N-1-naphthylphthalamic acid）对侧根形成的影响; 同时采用RT-PCR检测了NAC1和miR164在缺磷胁迫下的时序表达。结果表明,缺磷胁迫下侧根大量形成与生长素及其运输密切相关,在侧根迅速形成的24 h内,NAC1的表达在缺磷胁迫下增强; 而其上游的miR164表达降低,从而揭示了缺磷胁迫下侧根形成与miR164调节NAC1表达之间的关系。  相似文献   

Callus Induction of Aloe vera L var chinensis (Haw) Berg     

PAN Yu  HU Zong-li  CHEN Guo-ping  LIU Yang  WANG Xiao-yun  CHEN Xu-qing 《保鲜与加工》2007,(5):113-118

Studies were given to the induction and growth of callus of Aloe vera L.var.chinensis(Haw.) Berg when the young stem was applied as explant.MS was taken as basic medium,added with various concentrations of auxins(IAA,IBA,NAA and 2,4-D),cytokinins(6-BA,KT and Zeatin) and the different combinations of these plant growth regulators.Results showed that the callus is barely induced by cytokinin alone,and the callus induction rates have significant difference when different concentrations of auxin and different combinations of auxin with cytokinin are used on the MS medium.In order to inhibit callus browning during culturing,various concentrations of vitamin C or active carbon are added into four combinations of auxin and cytokinin respectively,which can induce callus most effectively.The result showsd that vitamin C can alleviate callus browning more effectively than the active carbon.The most efficient medium for induction and growth of callus of Aloe vera L.var.chinensis(Haw.) Berg was MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D+10 mg/ L vitamin C,with which the callus induction rate can reach to 91.4% and callus grow vigorously.  相似文献   

细胞分裂素促进白杨派树种插穗生根作用的研究   总被引：3，自引：0，他引：3  

陈雪梅  刘玉军 《北京林业大学学报》1992,14(1):66-69

用细胞分裂素(Cyt)配合生长素(Aux)处理银白杨、毛白杨和山杨插穗的试验表明,增加Cyt有促进愈伤组织发育和不定根形成的作用。与Aux处理相比,Aux+Cxt处理可使愈伤组织生根时间从50d缩短至30d左右。在人工气候室条件下,山杨扦插生根率达85％。  相似文献   

植物生理调节剂对四种蔬菜硝酸盐积累和产量的影响   总被引：1，自引：0，他引：1       下载免费PDF全文

曾晓蓉  肖和艾  吴金水  李宝珍  李霞 《农业现代化研究》2011,32(3):371-375

通过盆栽试验研究了植物生理调节剂对4种叶菜类蔬菜硝酸盐含量和产量的影响。结果表明,对于第一季蔬菜,2种土壤中减量施肥喷施3类植物生理调节剂与NPK处理比较,小白菜硝酸盐含量显著降低,而其产量略有下降,油麦菜硝酸盐含量略有下降,但其产量基本相同。对于第二季蔬菜(仅喷施调节剂),2种土壤中各处理萝卜菜硝酸盐含量和产量基本相同;长沙土壤中各处理空心菜硝酸盐含量和产量变化较小,而宁乡土壤中第一季施NPK处理的空心菜硝酸盐显著高于其他处理。除宁乡土壤油麦菜收获后NPK处理土壤硝酸盐含量显著高于减量施肥外,其他3种蔬菜收获后各施肥处理土壤硝酸盐含量差异较小。说明施用植物生理调节剂可减少化肥施用,降低叶菜类蔬菜硝酸盐含量,同时可保持蔬菜产量。此外,蔬菜硝酸盐含量与蔬菜种类和土壤类型有关。  相似文献   

金丝小枣插条中外源激素与内源激素的关系   总被引：3，自引：0，他引：3  

郑先武  田砚亭 《北京林业大学学报》1995,17(4):44-49

该文从生理生化的角度对外源生长素（ＮＡＡ）在金丝小枣嫩枝插穗中的作用进行了研究。实验结果表明，外源生长素（ＮＡＡ）影响插条内的生理生化反应，大大提高了插条内源生长素（ＩＡＡ）的含量，同时能够维持内源脱落酸（ＡＢＡ）含量保持在一定的水平，调节了内源激素ＩＡＡ和ＡＢＡ两者之间的动态平衡，使得ＩＡＡ／ＡＢＡ值保持基本稳定；说明两者之间有着一定的协调作用，内源ＩＡＡ很大程序上影响形成层处细胞，使其分化出根原始细胞。  相似文献   

植物激素对高山红景天试管苗愈伤组织诱导的影响   总被引：2，自引：1，他引：2  

安桂花  许明子  李美善  南桂仙  刘宪虎 《延边大学农学学报》2007,29(3):153-156

以高山红景天试管苗的叶片和茎为外植体研究不同植物激素配比对愈伤组织诱导的影响，结果表明：在4种生长素（2，4-D，NAA，IAA，IBA）和3种细胞分裂素（6～BA，ZT，KT）的配合中，NAA和6-BA组合的培养基对高山红景天愈伤组织诱导的效果最佳；试管苗茎的愈伤组织诱导率高于叶片．  相似文献   

OVER- EXPRESSION OF AN AUX1 HOMOLOG TRIGGERS OVARY INITIATION IN VITRO OF POPULUS TOMENTOSA CARR.     

FAN Jin-hui CHEN Jing LI Jing GUO Wei ZHANG Xian-sheng 《山东农业大学学报(自然科学版)》2007,38(4):501-508

INTRODUCTIONPolar transport is one key feature of auxin action was believed as a positional signal plays a wide arrange ofroles in plantgrowth and development,such as the gravity response[1],the initiation of lateral roots[2],positiondetermination of leaf primordial[3,4],differentiation of flower primordial[5],the axis formation in developingembryos[6~8],the patterned differentiation of vascular tissues[9~11].Polar auxin transport(PAT)system contributes indole-3-acetic acid(IAA)thorough …  相似文献   

甜椒组培育苗技术     

李进  张雪峰  刘燕 《辣椒杂志》2011,(3):42-43,49

研究了甜椒组培育苗技术,结果表明MS＋BA0．6mg／L＋IAA0．1mg／L配方接种,甜椒不定芽增殖系数达6．0,并且芽生长健壮;1／2MS＋NAA0．1mg／L配方的生根效果最好,15d生根率达71．9％,20d后生根率达100％。根系生长发达。  相似文献   

生长素对迷迭香插条生根影响研究初报   总被引：1，自引：0，他引：1  

李先芳  程茂高  李利红 《郑州牧业工程高等专科学校学报》2012,32(2):12-13,22

用IBA、2,4-D、ABT等3种生长素的不同质量水平对迷迭香当年生枝条分别进行绿枝和顶芽扦插,于沙床上全光照喷雾管理。用SPSS统计软件10．0对试验数据进行分析。结果表明3种生长素对迷迭香生根和成活有促进和提高作用。不同生长素的有效质量范围和作用效果存在差异。其中2,4-D表现最优,有效质量水平为1．0～2．0mg／L,质量水平越低,生根效果越好;其次为IBA,有效质量水平0．5～1．0mg／L,质量水平越高,生根能力越强;ABT最弱。  相似文献