A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen     

M. A. Dita  C. Waalwijk  I. W. Buddenhagen  M. T. Souza Jr  G. H. J. Kema 《Plant pathology》2010,59(2):348-357

This study analysed genomic variation of the translation elongation factor 1α (TEF‐1α) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR‐based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies.  相似文献   

藜草花叶病毒外壳蛋白基因的克隆与RT-PCR检测     

陈青  李桂芬  董菁  陈红运  廖富荣  陈洪俊  朱水芳 《植物病理学报》2010,40(4):438-441

 The nucleotide sequence of coat protein (cp)gene of Sowbane mosaic virus (SoMV)was determined. The cp gene of SoMV consists of 726 nucleotides and encodes a putative protein of 241 amino acid re-sidues. Sequence comparison showed that SoMV was most closely related to Rubus chlorotic mottle virus compared to other sobemoviruses. A primer pair was designed for the detection of SoMV based upon the determined cp nucleotide sequence. An expected fragment with 510 bp could be obtained from SoMV, while no specific band was observed from the healthy control. The result showed that the RT-PCR assay with the primer pair was suitable for the specific detection of SoMV.  相似文献   

进境澳大利亚油菜籽中茎基溃疡病菌的检测   总被引：1，自引：0，他引：1  

易建平  周国梁  印丽萍  陈仲兵  郑建中 《植物病理学报》2010,40(6):628-631

 41 fungal isolates with similar morphological characteristics to Leptosphaeria maculans were obtained by the deep-freezing filter paper method from 2100 seeds of Brassica napus imported from Australia.The isolate 8129-5 showed a slower growth on PDA at 20℃with growth rate of 2.8 mm/day.The colonies on PDA at 20℃ had an irregular or regular margin with white or grayish white compact aerial mycelium.No diffusible pigment was produced on PDA at 31℃ or in liquid Czapek-Dox media at 20℃.PCR detection showed that the isolate 8129-5 could be amplified by L.maculans-specific primers LmacF/LmacR and got expected product of 331 bp.The sequence analysis revealed that the ITS sequence of isolate 8129-5 had 99.8% identity with L.maculans.Pathogenicity of the isolate 8129-5 was confirmed on cotyledons of rape seed by artificial inoculation compared with typical symptom of L.maculans.Based on the morphological characteristics, PCR detection and the result of pathogenicity test, the isolate 8129-5 was identified as L.maculans.  相似文献   

河北省番茄黄化曲叶病毒病的分子鉴定初报   总被引：5，自引：1，他引：4  

周莹  李兴红  刘建华  姜京宇  王曙峰  燕继晔 《植物保护》2010,36(1):60-64

双生病毒是一类具有孪生颗粒形态的植物单链病毒,目前双生病毒病害已在多个国家和地区的作物上造成严重危害。近年来,我国多省报道作物上有这类病毒的发生,且有逐年加重和扩散的趋势。根据危害番茄的双生病毒DNA A序列保守区设计简并引物,对2009年4月采自河北省魏县表现叶片黄化、曲叶症状的4个番茄样品进行PCR检测,均为双生病毒阳性,对样品的扩增片断进行了克隆测序,经序列比对分析,与番茄黄化曲叶病毒（Tomato leaf curl virus,TYLCV）山东分离物（FJ646611.1）序列相似性为99.25%~99.55%,说明河北番茄黄化曲叶病由TYLCV引起。  相似文献   

水稻白叶枯病菌在离体培养条件下生物膜形成的检测   总被引：3，自引：1，他引：2  

傅本重  吴茂森  陈华民  何晨阳 《植物保护》2010,36(1):47-50

分析了不同培养和测试条件(塑料和玻璃表面、培养时间、培养温度和培养基)对水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae,Xoo)生物膜形成的影响,并测定了不同地区来源的Xoo野生型菌株、基因缺失突变体的生物膜形成。结果表明,在聚苯乙烯表面生长、用M210培养基在23 ℃下静止培养24 h是Xoo生物膜形成比较适宜的条件;不同地区来源的菌株生物膜形成能力不同;鞭毛相关基因fleQxoo、fliExoo和rbfCxoo以及H2O2降解调控基因oxyRxoo的缺失突变均影响生物膜形成。  相似文献   

RT-LAMP技术检测菜豆荚斑驳病毒的研究   总被引：3，自引：0，他引：3  

闻伟刚  杨翠云  崔俊霞  张颖 《植物保护》2010,36(6):139-141

菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)是我国对外公布的检疫性有害生物,本研究采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP),建立了一种快速、灵敏和特异的BPMV检测方法。根据BPMV外壳蛋白编码基因上的8个位点,共设计了6条引物,通过RT-LAMP扩增得到特征性的瀑布状条带。特异性试验表明,引物对BPMV的检测具有良好的特异性;灵敏度试验显示RT-LAMP比RT-PCR灵敏度高1 000倍。该方法无需特殊的试剂和设备,只需在水浴锅中60℃等温扩增,整个检测周期约2~2.5 h,适合BPMV的快速、准确检测。  相似文献   

杀菌剂抗性监测研究进展   总被引：1，自引：0，他引：1  

马琳  占绣萍  平新亮 《农药科学与管理》2010,31(8):24-28

使用现代选择性杀菌剂防治植物病害是保证农作物高产、稳产的重要措施之一,但是现代杀菌剂很容易出现抗性而导致化学防治失败．使农业生产蒙受巨大损失。本文从杀菌剂的抗性检测和治理技术等方面概述杀菌剂的抗性研究进展。  相似文献   

猪肉储藏时间的电子鼻区分方法   总被引：4，自引：0，他引：4  

洪雪珍  王俊  周博  王永维 《浙江大学学报(农业与生命科学版)》2010,36(5):568-572

用电子鼻对不同储藏时间(0~6 d)的猪肉样品进行区分,并确定电子鼻区分不同储藏时间肉品的较佳实验参数.对传感器信号进行多因素方差分析(analysis of variance,ANOVA)表明:对于固定容器的猪肉样品,不同的样品质量对电子鼻响应信号的影响极为显著;其次是样品在烧杯内的密封时间.通过单因素方差分析和区分度指标检验,得出猪肉在500 mL烧杯内的最佳参数为样品质量10 g、密封时间5 min.采用以上参数,对储藏0~6 d的猪肉样品进行辨别,结果发现不管是运用主成分分析(principle components analysis,PCA)还是线性判别分析(linear discri minant analysis,LDA),电子鼻都能很好地区分不同储藏天数的猪肉样品.  相似文献   

跟踪虫算法在烤烟烟叶边界检测中的研究   总被引：2，自引：0，他引：2  

李彰  毛鹏军  王俊  张伏  邱兆美  杜东亮 《农业科学与技术》2010,11(4):10-11,120

烟叶的形状包括烟叶的长度、宽度、面积、周长和圆度等叶形参数,只有准确获得烟叶的边界轮廓信息,才能计算众多的叶形参数。文章介绍了经典的边缘检测算子,并利用跟踪虫算法准确提取烟叶的边界轮廓特征。结果表明,该算法具有很好的边界提取能力。  相似文献   

Development and Validation of a Recombinant Envelop Glycoprotein Based Indirect Enzyme-Linked Immunosorbent Assay to Detect the Antibody to REV     

ZHANG Wen-juan  PEI Zong-fei  SHI Feng  NIU Xing  NIU Zhong-xiang 《中国农业科学(英文版)》2010,9(3):432-439

Based on the antigenic analysis of Reticuloendotheliosis virus （REV） envelop glycoprotein （env） protein, an alternative indirect enzyme-linked immunosorbent assay （ELISA） for detection of REV antibody was developed using a truncated env protein of REV produced in Escherichia coli. This assay was validated by comparison with an indirect immunofluorescence assay （IFA） and a REV-based ELISA. The diagnostic sensitivity （DSN）, specificity （DSP） and accuracy of the env indirect-ELISA （ienv-ELISA） were 93.7, 93.3 and 93.6% compared with IFA on 1 380 field serum samples, and 84.8, 95.2 and 91.7% compared with the REV-based ELISA on 96 field sera, respectively. Cross-reactivity assay showed that this assay was REV-specific. Repeatability test revealed that the coefficients of variation of positive sera within and between runs were less than 15%. This method is simpler to produce and perform, time-saving and suitable for large scale survey of REV antibody at low cost.  相似文献