Effects of gelatin prepared from calf bones rich in phosphorus on broiler performance,bone characteristics and digestive enzymes activity     

F. Beyranvand  A. Zamani  M. Manafi 《British poultry science》2019,60(1):31-38

1. Gelatin prepared from calf bones (GCB) is a novel source of high-quality protein and phosphorus. Its inclusion in broiler chicken diets may improve bone strength, plasma and digestive alkaline phosphatase activity (ALP), phosphorus digestibility and performance of broilers. Therefore, di-calcium phosphate in a corn-soy control diet was replaced with 12, 24, and 36 g/kg of GCB in a completely randomised design with four treatments of six replicates and 10 chicks in each pen. The trial lasted from 1 to 42 d of age.

2. Body weight and feed intake were measured weekly. Plasma calcium and phosphorus concentration along with plasma and digestive ALP were assayed throughout the trial. Trypsin, α-amylase, lipase and total protease activity were assayed at 14 and 28 d of age. Tibia ash, calcium and phosphorus content and breaking strength were measured at 14, 28 and 42 d of age. Phosphorus digestibility was measured at 36 d of age.

3. Body weight and feed intake showed no significant differences between controls and diets containing 12 and 36 g/kg GCB. Tibia ash and tibia length were increased by supplementation of GCB (P ≤ 0.001). Tibia calcium and phosphorus content were increased by GCB inclusion at 14 d of age (P ≤ 0.001). Digestive alkaline phosphatase activity was increased and trypsin activity was reduced by inclusion of GCB (P ≤ 0.001; P ≤ 0.004). α-amylase activity decreased by inclusion of 12 and 24 g/kg GCB, whereas an increase in α-amylase activity was observed by inclusion of 36 g/kg GCB (P ≤ 0.001). Supplementation of diets with GCB increased phosphorus digestibility (P ≤ 0.01) and suppressed ileum growth during the experimental period.

4. Results of the current study showed that phosphorus from gelatin can greatly improve broiler bone characteristics and phosphorus digestibility and complete elimination of inorganic phosphate sources from broiler diets is feasible with inclusion of 36 g/kg high phosphorus gelatin.  相似文献   

The in vitro antioxidant properties of alcalase hydrolysate prepared from silkie fowl (Gallus gallus) blood protein          下载免费PDF全文

Fu‐Yuan Cheng  I‐Chun Lai  Liang‐Chuan Lin  Ryoichi Sakata 《Animal Science Journal》2016,87(7):921-928

Two types of proteins including blood plasma protein and blood cell protein were isolated from silkie fowl (Gallus gallus) blood and hydrolyzed using alcalase for 0, 2, 4 and 6 h. The blood plasma protein hydrolysate (BPH) and blood cell protein hydrolysate (BCH) were analyzed for pH value, peptide content and antioxidative properties. The significantly higher peptide contents were observed in BPH than that of BCH, which showed that blood plasma protein was more suitable to hydrolysis by alcalase than blood cell protein. Both BPH and BCH showed strong 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical‐scavenging activity and Fe²⁺ chelating ability. BPH at 4 h of hydrolysis (BPH4) demonstrated significantly higher antioxidant capacity than those treated by alcalase in most of the assays. The BPH4 was separated using ultra‐filtration and assessment of the fractions and indicated that low molecular weight of peptides (< 3 kDa) possessed greater DPPH scavenging activity, Fe²⁺ chelating ability and inhibitory activity of lipid peroxidation. These results show that BPH has the potential to be ingredients in the food industry as a replacement of synthetic antioxidants.  相似文献   

载药骨基质明胶治疗骨髓炎的实验研究   总被引：3，自引：0，他引：3  

游洪波  黄瑛 《湛江医学院学报》2000,18(1):1-3

目的：探讨载药骨基质明胶（ＢＭＧ）治疗骨髓炎的可能性。方法：将ＢＭＧ溶于头孢唑林钠溶液，通过真空吸附、冻干等处理，制得载药ＢＭＧ。测定其体内、体外释药浓度及持续时间以及载药ＢＭＧ诱导成骨的能力。结果：载药ＢＭＧ对金葡菌的抑制作用体外为２２ｄ，体内为１４ｄ。载药ＢＭＧ体内释药时，局部组织浓度高，血浆浓度低；局部组织早期浓度高，以后为稳定的低浓度释放。载药ＢＭＧ中掺入抗生素后不影响ＢＭＧ的诱导成骨能力  相似文献   

酶解血粉饲料的发酵工艺及饲喂畜禽效果     

彭洪光  胡辅华 《华北农学报》1988,(4)

应用生物自显影术复壮和优选酶解血粉饲料生产用菌种,改良发酵载体配方和发酵工序,经化验分析和饲喂畜禽试验证实,产品质量提高,经济效益良好.  相似文献   

Treatment of Gelatin Production Wastewater with Photosynthetic Bacteria     

《保鲜与加工》2002,(6):44-47

  相似文献   

Muscle Protein Hydrolysates and Amino Acid Composition in Fish     

Bomi Ryu  Kyung-Hoon Shin  Se-Kwon Kim 《Marine drugs》2021,19(7)

Fish muscle, which accounts for 15%–25% of the total protein in fish, is a desirable protein source. Their hydrolysate is in high demand nutritionally as a functional food and thus has high potential added value. The hydrolysate contains physiologically active amino acids and various essential nutrients, the contents of which depend on the source of protein, protease, hydrolysis method, hydrolysis conditions, and degree of hydrolysis. Therefore, it can be utilized for various industrial applications including use in nutraceuticals and pharmaceuticals to help improve the health of humans. This review discusses muscle protein hydrolysates generated from the muscles of various fish species, as well as their amino acid composition, and highlights their functional properties and bioactivity. In addition, the role of the amino acid profile in regulating the biological and physiological activities, nutrition, and bitter taste of hydrolysates is discussed.  相似文献   

星点设计-效应面法优化硫氰酸红霉素肺靶向明胶微球的配方     

钱泽升  刘焕奇*  姜萍  高存帅  姜彩峰 《动物医学进展》2012,(9):84-88

用星点设计-效应面法优化硫氰酸红霉素明胶微球的处方,以期得到分散性好、粒径符合要求的明胶微球。本研究采用乳化-化学交联法制备,以明胶浓度、油水比例、乳化剂浓度为自变量,微球的平均粒径、载药量、包封率、跨距为因变量对自变量的各水平进行多元线性回归和二项式拟合。根据因变量效应面法选取较佳工艺,并在此基础上制备了硫氰酸红霉素微球,且进行了优化处方的验证。结果显示,二项式模型拟合效果较多元线性回归要好,最佳优化处方为明胶浓度0.156g/mL、油水比例12∶2、乳化剂浓度0.03g/mL,根据优化工艺制备的微球分散性好,平均粒径、载药量、跨距、包封率分别为12.51μm、21.28%、1.51和84.39%。体外释药特性研究表明,该微球符合一级方程规律,具有明显的缓释效果。通过星点设计-效应面法成功建立了处方优化模型,且预测性良好。  相似文献   

大豆蛋白在山葡萄酒澄清处理中的应用研究     

张艳  谢春阳 《当代生态农业》2010,(Z2)

大豆蛋白即大豆产品所含蛋白质,是植物性的完全蛋白质,绿色安全。本文研究了大豆蛋白对山葡萄酒的澄清作用。确定了大豆蛋白作为山葡萄酒下胶剂的最佳作用条件,即在作用温度:20℃;PH值:3.00条件下,添加0.06%大豆蛋白,以边加边搅拌,每天在振荡器中振荡1h,持续3d,静置,自然澄清的作用方式,使山葡萄酒的澄清度达到89%。并与常规下胶剂明胶进行比较。大豆蛋白比明胶的澄清作用、稳定性更强,口感也更加醇和。  相似文献   

Herring Milt and Herring Milt Protein Hydrolysate Are Equally Effective in Improving Insulin Sensitivity and Pancreatic Beta-Cell Function in Diet-Induced Obese- and Insulin-Resistant Mice     

Yanwen Wang  Sandhya Nair  Jacques Gagnon 《Marine drugs》2020,18(12)

Although genetic predisposition influences the onset and progression of insulin resistance and diabetes, dietary nutrients are critical. In general, protein is beneficial relative to carbohydrate and fat but dependent on protein source. Our recent study demonstrated that 70% replacement of dietary casein protein with the equivalent quantity of protein derived from herring milt protein hydrolysate (HMPH; herring milt with proteins being enzymatically hydrolyzed) significantly improved insulin resistance and glucose homeostasis in high-fat diet-induced obese mice. As production of protein hydrolysate increases the cost of the product, it is important to determine whether a simply dried and ground herring milt product possesses similar benefits. Therefore, the current study was conducted to investigate the effect of herring milt dry powder (HMDP) on glucose control and the associated metabolic phenotypes and further to compare its efficacy with HMPH. Male C57BL/6J mice on a high-fat diet for 7 weeks were randomized based on body weight and blood glucose into three groups. One group continued on the high-fat diet and was used as the insulin-resistant/diabetic control and the other two groups were given the high-fat diet modified to have 70% of casein protein being replaced with the same amount of protein from HMDP or HMPH. A group of mice on a low-fat diet all the time was used as the normal control. The results demonstrated that mice on the high-fat diet increased weight gain and showed higher blood concentrations of glucose, insulin, and leptin, as well as impaired glucose tolerance and pancreatic β-cell function relative to those on the normal control diet. In comparison with the high-fat diet, the replacement of 70% dietary casein protein with the same amount of HMDP or HMPH protein decreased weight gain and significantly improved the aforementioned biomarkers, insulin sensitivity or resistance, and β-cell function. The HMDP and HMPH showed similar effects on every parameter except blood lipids where HMDP decreased total cholesterol and non-HDL-cholesterol levels while the effect of HMPH was not significant. The results demonstrate that substituting 70% of dietary casein protein with the equivalent amount of HMDP or HMPH protein protects against obesity and diabetes, and HMDP is also beneficial to cholesterol homeostasis.  相似文献   

Properties and Composition of Concentrates and Syrup Obtained by Microfiltration of Saccharified Corn Starch Hydrolysate     

N. Singh  M. Cheryan 《Journal of Cereal Science》1998,27(3):315-320

Flow properties, clarity and composition of concentrates (retentates) and clarified syrup (permeates) obtained by membrane microfiltration of saccharified corn starch hydrolysate were determined. Clarified syrup (permeate) had low color and no suspended solids at all volume concentration ratios (VCRs) up to 100 X. The color and suspended solids in the concentrate increased linearly with increase in volume concentration ratio. Viscosity of the concentrates increased at higher VCR, but the viscosity of permeates remained essentially unchanged. The densities of all retentates and permeates were not significantly different from each other. Dextrose content of retentates and permeates at all VCRs did not change significantly during the microfiltration, averaging 28·90±1·01% w/w. Total solids, nitrogen, fat and ash content of the retentates increased with increase in VCR. In contrast, total solids, nitrogen, fat and ash of the permeates remained the same for all VCRs, averaging 29·44, 0·011, 0·07 and 0·042% respectively.  相似文献