Complexity of indica-japonica varietal differentiation in Bangladesh rice landraces revealed by microsatellite markers     

Mumu Wang  Zuofeng Zhu  Lubin Tan  Fengxia Liu  Yongcai Fu  Chuanqing Sun  Hongwei Cai 《Breeding Science》2013,63(2):227-232

To understand the genetic diversity and indica-japonica differentiation in Bangladesh rice varieties, a total of 151 accessions of rice varieties mostly Bangladesh traditional varieties including Aus, Boro, broadcast Aman, transplant Aman and Rayada varietal groups were genotyped using 47 rice nuclear SSRs. As a result, three distinct groups were detected by cluster analysis, corresponding to indica, Aus and japonica rice. Among deepwater rice varieties analyzed some having particular morphological features that mainly corresponded to the japonica varietal group. Some small seeded and aromatic varieties from Bangladesh also corresponded to the japonica varietal group. This research for the first time establishes that the japonica varietal group is a prominent component of traditional varieties in Bangladesh, particularly in deepwater areas.  相似文献   

Comparison of physiological and yield traits between purple- and white-pericarp rice using SLs     

Zhi-Juan Ji  Xiao-Guang Wang  Yu-Xiang Zeng  Liang-Yong Ma  Xi-Ming Li  Bing-Xin Liu  Chang-Deng Yang 《Breeding Science》2012,62(1):71-77

Five physiological and eleven yield traits of two pairs of sister lines generated from a high generation with similar genetic background (SLs) for purple pericarp were investigated to explore the reasons behind low-yield production of colored rice. Of the five physiological traits examined, except grain anthocyanin content, there were generally similar trends between the P (purple-pericarp) lines and the corresponding W (white-pericarp) lines over two seasons (in the year 2009 and 2010 separately). The results demonstrated that the chlorophyll content of flag leaves, the net photosynthetic rate of flag leaves, and the grain anthocyanin content could be easily influenced by the environment. The physiological functions of the traits for the P lines were more active than those of the corresponding W lines in the year 2010. The grain anthocyanin content of the P lines was much greater in the year 2010 than in the year 2009 during the growth period. The investigation of yield traits revealed that the P lines had reduced 1000-grain weight, yield per plot and grain/brown rice thickness compared to the W lines. A difference comparison of these traits and a source-sink and transportation relationship analysis for these SLs suggested that small sink size was a key reason behind yield reduction of purple pericarp rice.  相似文献   

基于SRAP的西瓜种质资源遗传多样性评价     

李朋飞  霍秀爱  程永强  代亮  杨炳艳  段会军 《中国农业科技导报》2013,15(2):89-96

运用SRAP分子标记技术分析了80份西瓜种质资源的遗传多样性，28对不同的引物组合共扩增出308条谱带，其中多态性谱带68条。引物组合的多态性频率最低为9.1%，最高为45.5%，平均22.1%。基于全部材料的SRAP基因型数据评价品种间的遗传关系，80份西瓜种质的遗传相似系数变化范围在0.94～1.00之间，表明供试西瓜种质资源的遗传背景比较狭窄。利用NTSYS软件聚类分析，80份西瓜材料被划分为3个类群，类群间相似度较高。来源地不同的西瓜材料也被划分到一个类群，进一步说明供试材料遗传背景狭窄。西瓜育种要取得突破性进展，必须加强种质资源的引进与创新。  相似文献   

Plant monocultures produce more antagonistic soil Streptomyces communities than high-diversity plant communities     

《Soil biology & biochemistry》2013

Plant–soil feedbacks are important to productivity and plant community dynamics in both natural and managed ecosystems. Among soil bacteria, the Streptomyces possess particularly strong antagonistic activities and inhibit diverse plant pathogens, offering a clear pathway to involvement in plant–soil feedbacks. We hypothesized that feedback effects and the ability of individual host plant species to foster antagonistic Streptomyces populations may be modified by the richness of the surrounding plant community. To test this, we collected soil associated with four different plant species (two C4 grasses: Andropogon gerardii, Schizachyrium scoparium; and two legumes: Lespedeza capitata, Lupinus perennis), grown in communities that spanned a gradient of plant species richness (1, 4, 8, 16, or 32 species). For each of these soils, we characterized the potential of soil Streptomyces to antagonize plant pathogens, using an in vitro plate assay with indicator strains to reveal inhibition. We cultivated each plant species in each conditioned soil to assess feedback effects on subsequent plant growth performance. Surrounding plant richness modified the impacts of particular plant species on Streptomyces antagonistic activity; A. gerardii supported a higher proportion of antagonistic Streptomyces when grown in monoculture than when grown in 32-spp plant communities, and L. capitata supported more strongly antagonistic Streptomyces when grown in 4- or 32-spp plant communities than in 8-spp plant communities. Similarly, the feedback effects of particular plant species sometimes varied with surrounding plant richness; aboveground biomass production varied with plant species richness for A. gerardii in L. perennis-trained soil, for L. capitata in A. gerardii-trained soil, and for L. perennis in L. capitata-trained soil. Streptomyces antagonist density increased with overall Streptomyces density under low but not under high plant richness, suggesting that plant diversity modifies selection for antagonistic phenotypes among soil Streptomyces. This work highlights the complexity of feedback dynamics among plant species, and of plant–microbiome interactions in soil.  相似文献   

Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-la and Genetic Variation of ORF5 Gene     

CAI Xue-hui  WU Guo-jun  LIU Yong-gang  LIU Guang-qing  SHI Wen-da  WANG Shu-jie  MAPing  LI Cheng-jun  HAN Wen-yu 《中国农业科学(英文版)》2012,(12):2035-2042

  相似文献   

橡胶树抗寒种质遗传多样性分析   总被引：2，自引：1，他引：1  

王其同  安泽伟  胡彦师  方家林  黄华孙 《热带作物学报》2012,32(2):11-14

应用RAPD和ISSR技术对20份橡胶树抗寒种质进行遗传多样性分析。结果表明，16个RAPD引物和12个ISSR引物分别扩增出条带113和101条，多态性条带指数(PPB)分别为61.1%和63.4%。据Nei-Li相似系数，ISSR标记在相似系数约0.74处，可将20份材料分为野生种质和栽培种质两大类；RAPD标记在相似系数约0.70处，也可将供试材料分为野生种质和栽培种质两类，但只有XJ002420为野生种质。对2种标记的分析结果进行相关分析，结果表明，RAPD和ISSR所检测的遗传相似系数呈极显著正相关，相关系数为0.672。以上结论表明，RAPD和ISSR标记可用于橡胶树种质资源的遗传多样性研究，但鉴于ISSR较RAPD有更强的多态性检出能力，ISSR技术应作为遗传多样性研究的首选单引物标记。  相似文献   

参数可变自适应遗传算法在军事地形图矢量化中的应用     

刘思力  潘伟 《湖南农业大学学报(自然科学版)》2010,(3):124-127

针对纸制军事地图矢量化问题进行研究，提出一种基于自适应遗传算法的军事地形图矢量化方法，并在生成新的个体后，增加个体修正操作，提高个体的适应度。在该算法中，提出一种即考虑进化代数对算法的影响，又考虑到每代不同个体适应度作用的自适应交叉概率和变异概率。通过与传统方法的实验比较，证明该方法的可用性和有效性。  相似文献   

贵州地方猪BF基因的群体遗传特性分析   总被引：1，自引：0，他引：1  

陈玉珍  刘若余  杨远青  王禄超  李敬瑞  惠嫣婷 《畜牧与兽医》2011,43(1)

以BF基因为候选基因,利用PCR和直接测序技术对贵州白香猪、可乐猪、大约克猪3个品种共95个个体BF基因外显子15、16、17的733 bp进行多态性分析,并对不同品种进行群体遗传学分析。结果显示:在第16外显子45 bp处存在A→C的碱基突变,命名为BF-exon16-A45C,导致编码的氨基酸由谷氨酸(Glu)突变为天冬氨酸(Asp);在贵州白香猪和可乐猪群体内均检测到AA、AC和CC三种基因型,在大约克猪群体内只检测到AC和CC两种基因型,贵州白香猪处于低度多态,可乐猪和大约克猪处于中度多态。经χ2适合性检验表明:贵州白香猪在该位点偏离了Hardy-Weinberg遗传平衡状态,可乐猪和大约克猪均处于Hardy-Weinberg遗传平衡状态。  相似文献   

小麦醇溶蛋白组分的遗传研究     

蔡岳 《北京农业》2011,(36):20-21

选取杂交组合(晋麦13×晋农190)通过A-PAGE技术并结合统计分析,分析了单个醇溶蛋白谱带在杂交后代中的遗传规律。醇溶蛋白谱带遗传在F2代表现为:6条谱带的分离(出现/缺失的比率)符合一对基因的分离规律,它们分别是13.9、23.2、26.0、40.2、50.0和71.5;5条谱带的分离符合两对有互补作用的基因的分离规律,它们分别是12.9、15.7、17.8、32.2、76.8,另外有2条谱带的分离符合两对独立基因的分离规律,它们分别是16.5、19.1。  相似文献   

鸡Lpin1基因3′-UTR遗传变异及其对miRNA结合位点的潜在效应     

李素雅  张建宏  陈文  黄艳群  孙桂荣  韩瑞丽  康相涛 《中国农业科学》2012,45(8):1613-1620

【目的】研究鸡Lpin1基因3′-UTR遗传变异/单倍型及其在品种间的分布，并预测这些变异对miRNA结合位点的潜在效应。【方法】根据鸡Lpin1基因组序列（GenBank登录号：NC_006090）设计一对特异性引物，采用PCR直接测序结合克隆测序的方法，进行鸡Lpin1基因3′-UTR及其邻近外显子在品种间的遗传变异/单倍型分析。【结果】①从6个品种中发现了8个变异位点，包含两个插入/缺失变异。这些变异均位于鸡Lpin1基因的3′-UTR，3′-UTR的变异频率为17SNPs/kb；②在鸡Lpin1基因3′-UTR区域，从固始鸡、卢氏绿壳蛋鸡中分别检测到7个变异位点，未检测到河南斗鸡品种内的变异；③用次要等位基因频率≥5%的6个变异位点（g.11A＞T，g.77C＞G，g.108_109delinsG，g.110_111delinsG，g.270A＞G和g.348G＞T）进行单倍型的构建和分析，从6个品种鸡中共检测到6种单倍型，其中P1和P4单倍型是群体的优势单倍型，频率均大于30%，河南斗鸡仅包含一种单倍型；④g.77C＞G变异可引起多个miRNA结合位点的增加/丢失。【结论】鸡Lpin1基因3′-UTR具有丰富的变异，3′-UTR变异位点/单倍型在品种间的分布存在明显的差异。  相似文献