烤烟废弃鲜叶作为沼气发酵原料的适用性研究     

李亚纯  朱红根  彭桃军  段史江  胡蓉花  史文强  彭以昌 《湖南农业科学》2014,(17)

烤烟废弃鲜烟叶降解较为困难,田间处理不当易造成环境污染和病害传播,为探索烤烟废弃鲜叶的无害化处理、资源化利用方式,试验对比了烤烟废弃鲜叶、烤烟废弃鲜叶加牛粪、烤烟废弃鲜叶加碳铵(NH4HCO3)、稻草秸秆分别作为发酵原料的产气周期和产气率等参数。结果表明,在不进行原料预处理时,烤烟废弃鲜叶可以作为沼气发酵原料正常生产沼气,自然条件下,产气周期在170 d以上、原料产气率在0.3 m3/kg以上;在发酵时添加适量的牛粪或碳铵,能进一步提高烤烟废弃鲜叶的发酵速度和产气率,并以加入鲜牛粪的效果更好。  相似文献   

JA_1对体外培养肝癌细胞线粒体膜电位及wt-p53基因表达的影响     

贾宁  方梅 《甘肃农业大学学报》2008,43(2):13-17

为探索梯度浓度的JA1对体外培养人肝癌细胞株(HCC-9724)的细胞周期、细胞线粒体膜电位和wt-p53蛋白表达的影响.采用流式细胞技术和体外细胞培养技术,发现经JA1处理后的细胞培养样本中有明显的DNA低含量颗粒("亚G1期"峰),细胞周期各时相分布发生改变,细胞在G1被阻滞.细胞线粒体膜电位(ΔΨm)明显下降,且表现出剂量和时间效应关系.而wt-p53蛋白阳性细胞百分率则随时间延长而逐渐增加.JA1诱导了HCC-9724细胞线粒体膜电位的下降和细胞凋亡,而wt-p53蛋白表达的增强则是其诱导HCC-9724细胞凋亡的重要分子机制之一.  相似文献   

西周金文考释二则     

吴红松 《安徽农业大学学报》2007,(2):108-111

本文在吸取前人说法的基础上, 结合古文献的记载, 从文字学角度对西周铜器铭文中的“ ”( ) 、“ ”二字重新进行考释; 认为“ ”( ) 仍释为“雍”, 但应指枲麻, 是明确古代服饰“巿”的质地之词;“ ”即古文献中的“裼”字, 指裼衣。后者在金文记载的服饰中为首见。此外, 本文还对这些服饰所涉及的西周社会制度略作论述  相似文献   

三峡库区蓄水172m水位线前后江面江岸鸳鸯越冬种群的分布调查   总被引：1，自引：0，他引：1  

苏化龙  肖文发  胥执清  刘富国  刘小云  张小蓉  王英 《西南大学学报(自然科学版)》2011,33(7)

2006-2009年,对三峡库区长江干流及12条主要支流的冬季水禽分布状况进行了较为系统的调查,其中鸳鸯的总体数量大约为100~120只.水位变动对鸳鸯的分布格局造成一定程度的影响,随着三峡大坝不同工程阶段的分期蓄水,三峡库区长江主河道的越冬鸳鸯种群数量呈明显减少趋势.2009年1月和2010年1月的调查结果表明,蓄水达172 m和168 m水位线后越冬鸳鸯集中分布于3条支流河道中,长江主河道中未见有越冬鸳鸯分布.  相似文献   

亚表层土壤修饰改性对镉离子的吸附影响     

孟昭福  张一平  张增强  王朝峰 《农业环境科学学报》2004,23(4):705-709

采用室内试验方法,研究了不同比例CTMAB单一修饰和CTMAB+SDS混合修饰的亚表层对金属镉离子的吸附影响。结果表明,吸附量顺序为CK>50%CTMAB>100%CTMAB≥120%CS,最佳吸附等温线模型可以用BET模型描述。热力学参数的研究表明,吸附自发性与吸附量具有相同的变化规律,反应的熵变对于反应的自发性具有决定作用。土样修饰改性后镉离子吸附的自发性减小,活性增加,淋溶性及对地下水的危害性增大。  相似文献   

水牛静脉注射葡萄糖后血糖水平的调节机制研究     

刘家国  刘艳娟  孙卫东  赵洪进  王小龙 《南京农业大学学报》2006,29(2):75-79

12头健康禁食公水牛分为3组。组Ⅰ(n=6)与组Ⅱ(n=3)分别经颈静脉快速注射50%葡萄糖和0.9%生理盐水各2 mL.kg-1;组Ⅲ(n=3)为对照,不作处理。分别于注射前60、10 m in及注射后1、5、10、30、60、120、180、240、300、360和420 m in采血,立即测定血浆葡萄糖及胰岛素和胰高血糖素,分析各指标间的相关性及血浆胰岛素/血糖值(IGR1)和胰岛素/胰高血糖素值(IGR2)。结果显示:注射前,各组水牛血浆葡萄糖、胰岛素和胰高血糖素的浓度接近。组Ⅱ和组Ⅲ水牛的上述指标在所有采样时间点上组内和组间均未表现出显著性差异(P>0.05),IGR1和IGR2也没有明显变化。组Ⅰ水牛静注葡萄糖1 m in后血糖浓度迅速升至峰值,而血浆胰岛素30 m in后才升高(约20倍)至峰值,然后两者均缓慢下降,直到420 m in仍明显高于注射前的水平(P<0.05)。而胰高血糖素仅在注射葡萄糖后1和5 m in时显示出差异(P<0.05),IGR1在注射葡萄糖(组Ⅰ)后立即下降,至注射后180 m in,组Ⅰ的IGR1和IGR2与组Ⅱ和组Ⅲ的基本持平。相关性分析表明,血糖浓度与血浆胰岛素水平呈极显著正相关(r=0.73,P<0.01),与胰高血糖素浓度呈显著负相关(r=-0.58,P<0.05),但胰岛素水平与胰高血糖素浓度相关性不显著(r=0.06,P>0.05)。提示:水牛胰岛素代谢迟缓,对静注的葡萄糖清除缓慢,表现出持续性高血糖症和高胰岛素血症;水牛对于静注葡萄糖的反应不同于奶牛、犬、马等其他动物。  相似文献   

Expression of late maturity α-amylase in wheat containing gibberellic acid insensitivity genes     

Kolumbina Mrva  Daryl J. Mares 《Euphytica》1996,88(1):69-76

Summary Two wheat cultivars, Spica and Lerma 52, which consistently produce high levels of mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase during the later stages of grain development (late maturity mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase), were crossed with a set of four near-isogenic lines carrying the tall (rht) allele or one of the dwarfing genes Rht1, Rht2 or Rht3 (GA-insensitive alleles). The F₁ and F₂ populations were developed and analysed for grain mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase and plant height. The Rht3 gene exhibited the strongest influence on plant height and strongly inhibited new mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase synthesis during the later part of grain ripening. By comparison, Rht1 and Rht2 had a less pronounced effect but still significantly reduced the expression of late maturity mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase. These observations suggest that gibberellic acid is involved either directly or indirectly in this phenomenon. The implications of the effect of dwarfing genes on expression of late maturity mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase are discussed in relation to cultivar improvement and to the identification and control of high mg src="/content/l381637p20m44327/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase germplasm.  相似文献   

Polymorphisms of α-amylase activity in barley landraces and cultivars from China     

Guanping Yang  Qifa Zhang 《Euphytica》1990,48(3):245-251

Summary mg src="/content/h14411r73128m01x/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-Amylase activity was assayed by measuring reducing power equivalent for 80 accessions of cultivated barley, Hordeum vulgare L., representing major barley growing areas of China. Replications were applied at two different levels of the experiment and enzyme activity was assayed on four consecutive days starting on the 6th day after germination. The area under the curve formed by connecting the four data points was integrated as the measurement of mg src="/content/h14411r73128m01x/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase activity. The results established that there was extensive variation in mg src="/content/h14411r73128m01x/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase activity in cultivated barley; about three-fold difference existed among accessions assayed. Comparisons were also made between six- and two-rowed, and between covered and naked barleys. The results showed that high mg src="/content/h14411r73128m01x/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase activity was not necessarily associated with six-rowed type, and that covered barleys were slightly higher in mg src="/content/h14411r73128m01x/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-amylase activity than naked ones.  相似文献   

Diagnosis and treatment of confirmed and suspected primary hyperparathyroidism in equids: 17 cases (1999–2016)     

E. B. Gorenberg  A. L. Johnson  K. G. Magdesian  F.-R. Bertin  L. R. R. Costa  M. J. P. Theelen  S. A. Durward-Akhurst  C. Cruz Villagrán  H. Carslake  N. Frank  J. E. Tomlinson 《Equine veterinary journal》2020,52(1):83-90

  相似文献   

猪m6A去甲基化酶FTO、ALKBH5基因表达水平与大肠杆菌F18感染抗性的关系分析     

齐晓艺  杨荔  吴正常  包文斌  吴圣龙 《中国畜牧兽医》2020,47(9):2960-2967

为了探讨猪6-甲基腺嘌呤（m⁶A）去甲基化酶mRNA表达水平与仔猪大肠杆菌F18抗性的关系,本研究运用实时荧光定量PCR方法检测m⁶A去甲基化酶FTO和ALKBH5基因在35日龄苏太猪断奶仔猪大肠杆菌F18抗性型和敏感型个体十二指肠和空肠组织中的mRNA表达差异,同时分别利用F18ab、F18ac大肠杆菌菌体刺激和内毒素LPS诱导猪小肠上皮细胞（IPEC-J2）,检测FTO、ALKBH5基因表达变化。结果表明,FTO、ALKBH5基因在抗性型个体十二指肠和空肠中的表达量均极显著或显著高于敏感型个体（P<0.01,P<0.05）;不同F18大肠杆菌菌体刺激IPEC-J2细胞后,FTO基因表达水平均无显著变化（P>0.05）,而ALKBH5基因在F18ac刺激后表达量显著上调（P<0.05）;LPS诱导4 h时,FTO和ALKBH5基因表达量均显著上调（P<0.05）。本研究在细胞和个体水平上初步验证并发现了m⁶A去甲基化酶FTO和ALKBH5基因表达水平与大肠杆菌感染仔猪密切相关,为今后深入研究RNA去甲基化修饰在仔猪细菌性腹泻调控中的作用机制提供了理论基础。  相似文献