赤芍水提物及芍药苷对大肠杆菌抗菌增敏活性的影响     

贾泽  王春光  张石磊  张鹏  张铁 《中国兽医学报》2019,(10):2026-2032

旨在考察中药赤芍水提物及其主要成分芍药苷对磷霉素钠的抗菌增敏活性,验证芍药苷对外排泵转运子AcrB的抑制作用,推测其抗菌增敏机制。首先,通过微量棋盘稀释法检测赤芍水提物及其主要成分芍药苷与磷霉素钠联合应用对临床分离的禽多重耐药大肠杆菌E320和质控菌株大肠杆菌ATCC35218的抑菌作用;其次,利用YASARA软件,以大肠杆菌多重耐药AcrAB-TolC系统中的外排泵转运子AcrB为靶标,与芍药苷进行分子对接模拟,探讨两者的识别机制;通过尼罗红外排试验验证芍药苷对外排泵的抑制作用;最后,通过实时荧光定量PCR验证芍药苷降低AcrB mRNA的表达量进而发挥抗菌增敏作用。试验结果证实赤芍水提物和芍药苷与磷霉素钠联合用药均具有协同抑制多重耐药大肠杆菌E320的作用(FICI≤0.5);分子对接结果显示芍药苷与AcrB蛋白受体的结合能达到8.333 kJ/mol,作用主要位点是AcrB蛋白受体上Phe628、Phe615、Val612、Ile277、Asn274、Gly179、Phe178等氨基酸残基,分子间主要作用力为疏水作用力;外排抑制试验结果显示芍药苷可以抑制耐药大肠杆菌外排泵活性,明显阻滞尼罗红的外排;实时荧光定量PCR结果显示芍药苷联合磷霉素钠比单独使用磷霉素钠显著降低大肠杆菌AcrB mRNA的表达量。本试验证明芍药苷通过作用外排泵转运子AcrB,使抗生素在菌体内积聚;并能显著降低AcrB mRNA的表达量进而起到抗菌增敏作用,为临床提供一种有效的抗菌增敏剂,对治疗耐药菌引起的细菌感染具有重要意义。  相似文献   

两色金鸡菊提取物对运动小鼠抗疲劳和耐缺氧作用的研究     

郝海燕  沙爱龙 《动物医学进展》2019,40(2):65-68

为研究两色金鸡菊提取物对小鼠的抗疲劳和耐缺氧作用,选择48只小鼠随机分为4组,即对照组,低、中、高剂量两色金鸡菊提取物组(每日5、10、20g/kg体重)。各组小鼠分别灌胃(每日0.20mL/10g)给予不同剂量的两色金鸡菊提取物,连续灌胃21d后,观察不同浓度的两色金鸡菊提取物对小鼠负重游泳时间及运动后小鼠血清尿素氮、血乳酸、肝糖原含量和小鼠常压耐缺氧存活时间、断头后呼吸维持时间的影响。结果表明,两色金鸡菊提取物能延长小鼠负重游泳时间,降低运动小鼠血清尿素氮、血乳酸含量,增加运动小鼠肝糖原含量,明显延长小鼠常压耐缺氧存活时间及断头后呼吸维持时间。结果提示,一定剂量的两色金鸡菊提取物具有抗疲劳作用和提高耐缺氧能力作用。  相似文献   

Effects of different levels of dietary protein with or without plant extract YGF251 on growth performance,nutrient digestibility,blood profiles,fecal microbial shedding,and fecal gas emission in growing pigs     

Xin Jian Lei  Sang In Lee  In Ho Kim 《Animal Science Journal》2019,90(4):547-553

This experiment was conducted to evaluate the effects of plant extract YGF251 supplementation in different protein level diets on growth performance, nutrient digestibility, blood profiles, fecal microbial shedding, and fecal gas emission in growing pigs. A total of 144 pigs (24.72 ± 1.54 kg) were randomly assigned to the treatments in a 2 × 3 factorial arrangement of dietary protein levels (15.50%, 14.00% or 12.50%) and plant extract YGF251 levels (0 or 0.05%) with 6 replications per treatment and 4 pigs per pen. Pigs fed low protein diets had reduced average daily gain (p < 0.05) and increased feed conversion ratio (p < 0.01) compared with pigs fed high protein diets. The apparent total tract digestibility of nitrogen was decreased (p < 0.05) when reducing dietary protein level. Fecal ammonia and hydrogen sulfide emissions were reduced (p < 0.05) when reducing dietary protein level. In conclusion, the results of the current study indicated that reducing dietary protein level impaired growth performance and nitrogen digestibility but reduced ammonia and hydrogen sulfide emissions in growing pigs. Dietary supplementation with 0.05% herbal extract YGF251 was not effective in improving growth performance, nutrient digestibility, or in decreasing gas emission in different protein diets.  相似文献   

UPLC-PDA法测定蒲公英提取物中咖啡酸的含量     

魏秀丽  徐恩民  刘霄飞  李有志  张秀英  王海军- 《中国兽药杂志》2019,53(3):26-30

建立超高效液相色谱-二极管阵列检测法(UPLC-PDA法)测定蒲公英提取物中咖啡酸的含量。采用反相高效液相色谱法,用带有二极管阵列检测器的高效液相色谱仪(UPLC-PAD)对咖啡酸进行色谱分离和快速筛查。以ACQUITY UPLCTMHSS T3色谱柱(2.1 mm×100 mm,1.8μm)为分离柱,柱温:35℃;流动相体系:A项为乙腈,B项为0.4%磷酸水溶液,进行梯度洗脱;流速:0.35 m L/min;进样量:5μL;检测波长为323 nm。通过光谱图、保留时间及峰面积参数对咖啡酸进行定性定量检测。咖啡酸在1～100μg/m L的范围内线性良好(R2=0.999); 0.25μg/m L咖啡酸对照品溶液与空白溶液的信噪比3,为检出限,1μg/m L咖啡酸对照品溶液与空白溶液的信噪比10,为定量限,完全满足检测需求。该方法色谱分离较好,分析速度较快,前处理简单,适用于蒲公英提取物中咖啡酸的定性定量检测。  相似文献   

Ethyl acetate extract of Pleione bulbocodioides (Franch.) Rolfe induces apoptosis of human leukemia K562 and HL-60 cells through intrinsic mitochondrial apoptosis pathway     

HAO Gang-ping  HAO Jia-li  LI Yue  ZHAO Fa-xing  ZHANG Yuan-ying  JIANG Han-ming  YANG Zhong-fa 《园艺学报》2018,34(5):769-777

AIM:To investigate the effects of ethyl acetate (EtOAc) extract of Pleione bulbocodioides (Franch.) Rolfe on proliferation and apoptosis of human leukemia K562 and HL-60 cells and the possible apoptosis pathway. METHODS:Human leukemia cell lines were treated with EtOAc extract of Pleione bulbocodioides at different concentrations. XTT method was used to evaluate the viability of K562 cells and HL-60 cells. The cell growth inhibition was calculated by Trypan blue exclusion test. The percentage of apoptotic cells was determined by flow cytometry, and 4',6-diamidino-2-phenylindole (DAPI) was used to observe morphological changes of the cells. The cell cycle was observed by propidium iodide (PI) staining. The protein expression of Bcl-2, Bax, cleaved poly(ADP-ribose) polymerase (PARP), cleaved caspase-3, cytochrome C and apoptosis-inducing factor (AIF) wase determined by Western blot. RESULTS:The cell viability and proliferation were inhibited by EtOAc extract of Pleione bulbocodioides with IC₅₀ of (42.14±2.54) mg/L for HL-60 cells and (51.28±3.12) mg/L for K562 cells at 24 h. The results of Annexin V-FITC/PI and DAPI staining showed that EtOAc extract of Pleione bulbocodioides induced cell apoptosis in a dose-dependent manner. The apoptotic rate was increased compared with control group (P<0.05). The G₂ phase increased with typical cell apoptosis-induced morphological changes. The levels of pro-apoptotic proteins Bax, cleaved PARP and cleaved caspase-3 were increased, while Bcl-2 was down-regulated (P<0.05). Cytochrome C and AIF in cytosol, characteristic proteins of intrinsic mitochondrial apoptosis pathway, also increased with the concentration of EtOAc extract of Pleione bulbocodioides increasing (P<0.05). CONCLUSION:EtOAc extract of Pleione bulbocodioides significantly inhibits cell proliferation and induces cell apoptosis in human leukemia cell lines HL-60 and K562 through intrinsic mitochondrial apoptosis pathway.  相似文献   

长期添加藤茶提取物对肥育猪血清游离氨基酸、胴体性状和肉品质的影响     

熊云霞  王丽  易宏波  吴绮雯  温晓鹿  胡友军  蒋宗勇 《中国畜牧兽医》2019,46(11):3252-3261

本试验旨在研究日粮中长期添加藤茶提取物对肥育猪血清游离氨基酸、胴体性状和肉品质的影响。选用平均初始体重为30 kg的三元杂交（杜×长×大）去势公猪90头,随机分成3个处理组,分别为对照组（CON组,饲喂基础日粮）、植物精油复合物组（A组,基础日粮中添加0.03%植物精油复合物）、藤茶提取物组（B组,基础日粮中添加0.03%藤茶提取物）,每组6个栏,每栏5头猪。试验猪体重达到130 kg左右出栏称重,每栏挑选最接近该栏平均体重的猪进行采血、屠宰,以A组为阳性对照。结果表明：①与CON组比较,B组的血清游离氨基酸中必需氨基酸赖氨酸（Lys）、蛋氨酸（Met）、异亮氨酸（Ile）等的含量显著升高（P<0.05）,组氨酸（His）的含量显著降低（P<0.05）,非必需氨基酸谷氨酸（Glu）、丙氨酸（Ala）、脯氨酸（Pro）等的含量显著降低（P<0.05）;与A组比较,B组的血清游离氨基酸中必需氨基酸Met、苏氨酸（Thr）含量显著升高（P<0.05）,His含量显著降低（P<0.05）,非必需氨基酸均不存在显著差异（P>0.05）。②与CON组比较,A、B组猪的腹脂重均显著提高（P<0.05）,但A、B组之间腹脂重不存在显著差异（P>0.05）;宰前重、胴体重、屠宰率、背膘厚、眼肌面积等3组间均无显著差异（P>0.05）。③宰后背最长肌肉色、pH、滴水损失、大理石评分、肌内脂肪和剪切力3组间均无显著差异（P>0.05）;与CON组比较,B组肌肉嫩度评分显著提高（P<0.05）,A、B组的肉汤清浊度评分均显著降低（P<0.05）,而肌肉外形、气味、异味、多汁性、肉汤鲜度评分在3组间无显著差异（P>0.05）;3组的肌纤维直径和密度均无显著差异（P>0.05）。综上,日粮中长期添加0.03%藤茶提取物可以显著改变肥育猪血清游离氨基酸组成,并一定程度改善猪肉嫩度;长期添加藤茶提取物或植物精油复合物对肥育猪胴体和肌肉性状均无显著改善效果,且均易造成腹部脂肪沉积。  相似文献   

华南双季稻化肥减施增效综合技术模式及技术规程     

韦善清  谢慧敏  吴可  梁和  江立庚 《中国农学通报》2021,37(36):1-5

水稻生产过程中化肥的不合理施用,带来了农产品生产成本增高、农田环境污染等问题,影响水稻生产的可持续发展。于2018—2020年,在单项技术研究基础上,开展本研究,旨在形成适合华南双季稻区的化肥减施增效综合技术模式。研究选用高产、高效水稻品种（双高）,同时优化氮、磷、钾肥的比例结构以及基蘖肥的比例结构（双优）,在化肥中补充施入海藻精和微生物菌剂（双补）,合理平衡移栽密度与肥料施用量、水气平衡方法水分管理（双平衡）的方法。2019—2020年在象州和宾阳县进行技术示范,化肥减量综合技术示范区的化肥施用量比对照减少化肥用量17%（包括氮、磷、钾肥）基础上,平均稻谷产量分别为6479.76 kg/hm²、6755.62 kg/hm²,其稻谷产量分别比对照增产2.30%和6.55%。本研究总结了华南双季稻化肥减施增效技术规程。  相似文献   

Effects of Chinese wolfberry and astragalus extracts on growth performance,pork quality,and unsaturated fatty acid metabolism regulation in Tibetan fragrant pigs     

Zhuang Hao  Zhen Li  Jinjin Huo  Yaocheng Chu  Jiandong Li  Xiaohong Yu  Fenghua Liu  Peng Yin 《Animal Science Journal》2021,92(1):e13581

  相似文献   

微泡技术在有机物分离和提取中的应用     

周春雨  孟令伟  杨林 《绿色科技》2014,(8):202-204

综述了微泡技术在分离、提取和降解有机物甲基叔丁基醚、多环芳烃、多氯联苯、活性淤泥、石油光泽几个方面的应用,与传统方法相比,微泡技术使甲基叔丁醚的利用率提高15倍;使多环芳烃和多氯联苯的去除时间缩短;使活性淤泥中较大固体减少缩短消除时间,并且增加能源生产;使含有有机酸的水溶液浊度从200NTU降低到2NTU,并且和表面光泽完全隔离。展望了微泡技术在色素或某类有机物的分离和提取中的应用,为色素或某类有机物的分离和提取提供更进一步的研究参考。  相似文献   

Integrated management of postharvest banana anthracnose (Colletotrichum musae) through plant extracts and hot water treatment     

《Crop Protection》2014

The aim of the present study was to determine the integrated effect of plant extracts and hot water treatment on postharvest anthracnose and banana fruit quality. Artificially inoculated banana fruit were dipped in 10% and 20% (w/v) extracts of four plant species, viz. Acacia albida, Argemone mexicana, Dovalis abyssinica and Prosopis juliflora at room temperature (20 °C), 45 °C and 50 °C. Fruit treated separately with 20% extracts of A. albida and P. juliflora at 50 °C had reduced anthracnose incidence and severity. These treatment combinations also had the highest percentage of marketable ripe banana fruits. In conclusion, applying aqueous plant extracts at 50 °C enhanced the antifungal activity of extracts without affecting the physico-chemical properties of banana.  相似文献