高效液相色谱法测定水产品中ATP关联化合物          下载免费PDF全文

汤水粉  钱卓真  罗方方  吴成业 《渔业科学进展》2014,35(2):110-116

建立了水产品中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、腺苷酸(AMP)、肌苷酸(IMP)、次黄嘌呤核苷(HxR)和次黄嘌呤(Hx)6种ATP关联化合物的高效液相色谱检测方法。样品中的ATP关联物经高氯酸提取并用氢氧化钠调节pH值至6.0-6.4后,以AQ-C18(250 mm×4.6 mm,5μm)柱为分析柱,0.02 mol/L KH2PO41-0.02 mol/L K2HPO4(V/V=1/1)的缓冲液为流动相进行洗脱,在254 nm波长下用高效液相色谱进行检测,外标法定量。研究表明,ATP、ADP、AMP、IMP和HxR线性范围为0.2-40.0μg/ml,Hx线性范围为0.1-20.0μg/ml,相关系数均大于0.999 0;ATP、ADP、AMP、IMP和HxR检出限为5.00 mg/kg,Hx检出限为2.5 mg/kg(S/N=3);对真鲷、鱼师鱼、鲑鱼、凡纳滨对虾和梭子蟹5种水产品进行3个浓度水平的加标实验,回收率在85.5%-105%之间,相对标准偏差(RSD)小于12.9%。实验结果表明,该方法简便、准确,适合于水产品中ATP关联物的测定。  相似文献   

水产品中食品添加剂限量标准分析及建议     

孟娣  宋怿  房金岑 《渔业现代化》2011,(5)

介绍CAC、美国、日本、欧盟等我国几个主要水产品出口对象的食品添加剂限量标准,并与我国相关标准要求进行了对比分析,探讨了我国现行水产品中食品添加剂限量标准存在的问题,提出了对策和建议。  相似文献   

Oxidative Stability of Common Kilka (Clupeonella cultriventris caspia) Oil Supplemented with Microwave Extracted Ghure (Unripe Grape) Marc Extract     

Mohammad-Taghi Golmakani  Malihe Keramat  Marzieh Moosavi-Nasab  Bahareh Moosavian 《Journal Of Aquatic Food Product Technology》2017,26(9):1022-1031

The objective of this study was to evaluate the effect of microwave extracted Ghure (unripe grape) marc extract on common kilka (Clupeonella cultriventris caspia) oil oxidation during accelerated storage. The antioxidant activity of Ghure marc extract was compared with those of α-tocopherol and BHT. The Ghure marc extract significantly reduced the peroxide and p-anisidine value of kilka oil. Even though the effect of Ghure marc extract on reducing the oxidation of kilka oil was similar to the effect of BHT, it was functionally more effective than α-tocopherol. Generally, Ghure marc extract could be considered as a potential source of natural antioxidants, with inexpensive costs, for improving the oxidative stability of kilka oil.

Abbreviations: AA, Antioxidant activity; AOP, Antioxidant power; AV, p-Anisidine value; CUPRAC, Cupric ion reducing antioxidant capacity; DHA, Docosahexaenoic acid; EPA, Eicosapentaenoic acid; FIC, Ferrous ion chelating; GME, Ghure marc extract; FRAP, Ferrous ion reducing antioxidant power; KO, Kilka oil; IP, Induction period; MAE, Microwave-assisted extraction; MUFA, Monounsaturated fatty acid; PUFA, Polyunsaturated fatty acid; PV, peroxide value; PF, Protection factor; RSA, Radical scavenging activity; SFA, Saturated fatty acids; TFC, Total flavonoid content; TPC, total phenolic content TV, totox value.  相似文献   

Natural bixin as a potential carotenoid for enhancing pigmentation and colour in goldfish (Carassius auratus)          下载免费PDF全文

S.H.S. Dananjaya  D.M.S. Munasinghe  H.B.S. Ariyaratne  J. Lee  M. De Zoysa 《Aquaculture Nutrition》2017,23(2):255-263

Discovering natural carotenoids for colour enhancement and health benefits of fish is important to develop new feed formulations. We have purified natural bixin from achiote seeds and evaluated the effect of colour enhancing and pigmentation in goldfish. Varying levels of bixin‐based diets were prepared with 420 g kg^?1 of crude protein and 120 g kg^?1 of lipid content. Our results clearly showed that bixin (0.05, 0.10, 0.20 and 0.60 g kg^?1) based diets significantly (P < 0.05) enhanced the skin and fin colour at 30 and 60 days compared to diet without bixin. Interestingly, diet which contains 0.20 g kg^?1 bixin and commercial feed (with astaxanthin) had similar effect on carotenoid deposition in skin. Moreover, total carotenoid deposition in fin was higher than in skin of all bixin‐containing diets. However, 0.60 g kg^?1 bixin‐containing diet had lower specific growth rate (1.01 ± 0.01) and higher feed conversion ratio (2.05 ± 0.19) compared to the control group. The present results demonstrate that achiote bixin can be successfully used as an alternative natural carotenoid source against synthetic astaxanthin in fish feed. Our data indicate that 0.20 g kg^?1 is a suitable dietary level of bixin to ensure strong pigmentation, acceptable growth and feed utilization in goldfish.  相似文献   

A compendium of raw material digestibilities for barramundi,Lates calcarifer          下载免费PDF全文

B. Glencross  D. Blyth  S. Cheers  N. Bourne  N. Wade  S. Irvin 《Aquaculture Nutrition》2017,23(5):1055-1064

A series of experiments were conducted to examine the nutrient and energy digestibility of a suite of diets and specific test raw materials when fed to juvenile (179 to 439 g) barramundi, Lates calcarifer. Each of the diets was prepared using a twin‐screw extruder to mimic modern aquafeed manufacturing processes. Each of the diets was fed to juvenile barramundi for a minimum of a week to allow acclimation to the diet before the faeces were collected using stripping methods. A broad range of digestible nutrient and energy values among the different raw materials were observed, with protein digestibilities ranging from 36% to 106% and energy digestibilities ranging from 36% to 93%. This range in nutritional values of the different raw materials provides substantial utility in allowing the formulation of diets on a digestible nutrient and energy basis across the Asia Pacific region. These results also provide critical data to help underpin the replacement of both fishmeal and fish oil in barramundi diets.  相似文献   

Anaesthetic and antioxidant effects of Myrcia sylvatica (G. Mey.) DC. and Curcuma longa L. essential oils on tambaqui (Colossoma macropomum)          下载免费PDF全文

Etiane M H Saccol  Cândida Toni  Tanise S Pês  Giovana M Ourique  Luciane T Gressler  Lenise V F Silva  Rosa H V Mourão  Ricardo B Oliveira  Bernardo Baldisserotto  Maria A Pavanato 《Aquaculture Research》2017,48(5):2012-2031

Anaesthetic substances are necessary to reduce fish stress during aquaculture activities. The objectives of this study were: (i) to determine the efficacy of essential oils (EOs) of Myrcia sylvatica (EOMS) and Curcuma longa (EOCL) as anaesthetics for Colossoma macropomum and (ii) to evaluate the effects of rapid anaesthesia and long‐term sedation (6 h) with these oils. Therefore, the main primary stress indicator (cortisol) and secondary factors (biochemical indices, hepatic metabolism, oxidative biomarkers) were measured. Sedation with the EOCL resulted in lower cortisol levels compared to control group. Total cholesterol levels were lower in fish sedated with EOMS than in control. Lactate levels were higher in fish anaesthetized with both EOs and sedated with EOCL compared to control. Both EOs increased hepatic glycogen levels after anaesthesia and EOMS increased this parameter after sedation compared to control. Anaesthesia and sedation with EOs resulted in lower levels of lipid peroxidation (LPO) compared to control. In turn, the activity of some antioxidant enzymes evaluated (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione‐S‐transferase), the content of non‐protein thiols and total reactive antioxidant potential were higher in tissues of fish anaesthetized and sedated with EOs compared to control. This induction of antioxidant capacity in the tissues could be due to the antioxidant property exerted by these EOs. Thus, EOMS and EOCL are recommended for anaesthesia and sedation of fish because in spite of inducing anaerobic metabolism, these EOs did not alter most biochemical parameters, reduced the LPO and increased the antioxidant capacity in vital tissues.  相似文献   

低碳经济引领水产品加工业发展新方向   总被引：4，自引：1，他引：3  

李来好  邓建朝  杨贤庆  岑剑伟  吴燕燕  戚勃 《水产学报》2011,35(4):636-640

阐述了目前水产品加工业的现状和存在问题,分别从加工科技含量,生产设备;高附加值产品开发,精深加工能力;产业结构、产品质量标准体系和法律法规等方面展开论述,探讨了制约我国水产品加工业低碳经济发展的因素,提出了水产品加工业向低碳经济发展的思路和相关措施。水产品加工业的低碳经济发展道路需要依靠技术创新,提高水产品副产物的综合应用,发展水产品精深加工。另外,国家政策引导和产业结构调整等措施对水产品加工业低碳经济可持续发展也至关重要。  相似文献   

西藏天然林资源保护工程现状及发展对策研究   总被引：2，自引：0，他引：2  

张敏 《林业科学》2005,41(2):186-190

1　天然林保护工程实施以来取得了明显的阶段性成果西藏是我国重要国有天然林区之一 ,现有有林地面积 75 1万hm2 ,约占国有林区天然林面积的 2 7 8% ,活立木蓄积量为 2 0 84亿m3 ,约占国有林区天然林蓄积量的 72 %。昌都地区的江达、贡觉、芒康 3县是我国长江流域的重要区域 ,  相似文献   

天敌昆虫对马尾松毛虫控制作用及其群落的研究   总被引：2，自引：0，他引：2       下载免费PDF全文

夏育陆  周健生  吴正坤 《林业科学研究》1992,5(3):356-360

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水产品中拟除虫菊酯类农药残留检测方法的研究   总被引：1，自引：0，他引：1  

姜琳琳 《福建水产》2009,(2):66-71

建立了水产品中氯氰菊酯、甲氰菊酯、氰戊菊酯和溴氰菊酯等拟除虫菊酯类农药残留的提取、净化以及毛细管气相色谱的测定方法。选择40mL正己烷：丙酮（V／V,2：1）混合溶液作为提取液,经冷冻去脂,柱层析净化过程中选用3g中性氧化铝作为净化填料,30mL正己烷：丙酮（V／V,4：1）混合溶液进行洗脱,然后用气相色谱法测定,外标法定量。实验结果表明,在10～200ng／mL线性范围内线性良好,相关系数在0．9993～0．9998。标准品添加量在10～66μg／kg范围内,罗非鱼样品加标实验的回收率在76．9～90．3％,相对标准偏差在1．25—4．66％,样品中氯氰菊酯、甲氰菊酯、氰戊菊酯、溴氰菊酯残留量检测方法的检出限为10μg／kg。另外采用鳗鱼和对虾样品的标准添加回收实验进行方法的验证,实验结果表明,平均回收率达到70％以上,相对标准偏差小于10％,符合药残检测要求。本方法可用于进行水产品中多种菊酯类农药残留的检测分析。  相似文献