Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents     

Kanae SHIOKAWA  Chandika D. GAMAGE  Nobuo KOIZUMI  Yoshihiro SAKODA  Kenta SHIMIZU  Yoshimi TSUDA  Kumiko YOSHIMATSU  Jiro ARIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):221-230

The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then, truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared between two recombinant expression hosts Escherichia coli and Pichia pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris (tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E. coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results from flaB gene PCR of kidney samples and WB with whole Leptospira cell. Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32 expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of background reaction.  相似文献   

Role of social structure in establishment of an invasive large mammal after translocation     

Lindsay M. Clontz  Anni Yang  Sarah M. Chinn  Kim M. Pepin  Kurt C. VerCauteren  George Wittemyer  Ryan S. Miller  James C. Beasley 《Pest management science》2023,79(10):3819-3829

Background

Data on the movement behavior of translocated wild pigs is needed to develop appropriate response strategies for containing and eliminating new source populations following translocation events. We conducted experimental trials to compare the home range establishment and space-use metrics, including the number of days and distance traveled before becoming range residents, for wild pigs translocated with their social group and individually.

Results

We found wild pigs translocated with their social group made less extensive movements away from the release location and established a stable home range ~5 days faster than those translocated individually. We also examined how habitat quality impacted the home range sizes of translocated wild pigs and found wild pigs maintained larger ranges in areas with higher proportion of low-quality habitat.

Conclusion

Collectively, our findings suggest translocations of invasive wild pigs have a greater probability of establishing a viable population near the release site when habitat quality is high and when released with members of their social unit compared to individuals moved independent of their social group or to low-quality habitat. However, all wild pigs translocated in our study made extensive movements from their release location, highlighting the potential for single translocation events of either individuals or groups to have far-reaching consequences within a much broader landscape beyond the location where they are released. These results highlight the challenges associated with containing populations in areas where illegal introduction of wild pigs occurs, and the need for rapid response once releases are identified. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Using morphometrics to identify sex in the eastern blue-tongued skink (Tiliqua scincoides)     

A Bird  D Monks  IP Hughes 《Australian veterinary journal》2023,101(7):282-290

  相似文献   

摆动式花生收获机整体载荷的试验与分析   总被引：1，自引：0，他引：1  

尚书旗  刘曙光  梁洁  王方艳  姜元志  杨然兵 《农机化研究》2007,(11):173-176

从理论上建立了摆动式花生收获机的空间力系模型.在此基础上,围绕摆动式花生收获机的主要试验参数-传动速比、偏心距、栅条长度与前进速度的改变进行了摆动式花生收获机整体载荷的试验.试验证明:花生收获机在工作时所受载荷平衡,通过二次回归旋转组合试验测定整体载荷随4因素的变化规律是显著的,结合花生收获机的实际工作条件分析了回归方程的适用范围.本试验为摆动式花生收获机在功率消耗、力的平衡等方面的进一步改进提供了依据.  相似文献   

花生旋挖机概念设计     

张居敏  黄永俊 《农机化研究》2007,(9):131-133,136

分析了我国目前花生收获机械的优缺点,对其存在的原理性缺陷提出了改进措施.挖掘前进行割蔓处理,用旋挖铲代替传统的牵引式挖掘铲,辅之以红外线花生稞识别技术,可降低挖掘功耗,提高挖掘后的花生果筛分效果;同时,结合花生的物性特征对旋挖部件的运动学参数进行了详细地理论分析,并给出了理想旋挖机的作业方式及功能模块图,为改善目前花生挖掘机械的挖掘效果提供了全新的设计思路.  相似文献   

新型花生免耕施肥播种机研制     

王东升  肖伟中  王江涛  李伟  刘怀玉 《中国农机化学报》2020,(3):40-45

针对国内花生机械播种机普遍存在缠绕壅堵、出苗不均匀等问题,设计一种新型花生免耕施肥播种机。该机设计新型的破茬清茬防缠绕刀具,并采用同位施肥技术,可一次性完成秸秆粉碎、开沟播种、施肥、覆土镇压等作业。田间试验结果表明:该免耕施肥播种机通过性能良好,有效地解决了秸秆对缠绕壅堵问题。播种、施肥深度合格率和变异系数分别为96%、92%和19.5%、15.6%,作业质量符合农艺要求。  相似文献   

花生摘果机具的研究现状与性能分析   总被引：3，自引：2，他引：3  

王晓燕  梁洁  尚书旗  赵忠海  周亚龙 《农机化研究》2006,(11):39-42

通过对花生摘果装置与机具的调研和资料检索,简述了各种花生摘果装置与机具的类型及特点。在系统地论述和分析了花生摘果的主要方式及基本原理的基础上,从工作原理、结构特点及适应条件等方面介绍了现有4种类型花生摘果装置所具有的优势与存在的不足,阐述了花生摘果装置与机具在我国的研究应用现状,并客观地分析了花生摘果机具的发展动态。  相似文献   

花生根瘤愈伤组织的诱导和分化     

曹燕珍  李阜棣 《华中农业大学学报》1990,9(1):47-50,T001

生长健壮的花生根瘤在CLS培养基上诱导出愈伤组织，形成愈伤组织的频率达到26．5－46．6％。瘤愈伤组织能多次继代，生长良好，在分化培养基D－57和D－59上已分化形成了根，其生根频率分别为56％和78％。显微观察表明，愈伤组织分化的具有正常根的解剖结构。  相似文献   

播期对花生光合性能与产量影响的研究   总被引：5，自引：0，他引：5  

于旸  王铭伦  张俊  王月福  赵长星 《青岛农业大学学报(自然科学版)》2011,(1):16-19

以青花5号花生品种为材料,在田间地膜覆盖栽培条件下,系统研究了不同播期对花生光合性能和产量的影响。结果表明:不同播期对花生功能叶片光合性能和产量均有显著影响,叶面积系数、叶绿素SPAD值、净光合速率、产量均以4月30日和5月10日播种的较高,过早和过晚播种均不利于功能叶片光合性能和产量的提高。研究认为,在本试验条件下,5月上旬是花生获得高产的最佳播期。  相似文献   

花生壳残渣制备活性炭及吸附性能测定   总被引：1，自引：0，他引：1  

杨莉  谢宇  邱贤华  邓磊 《花生学报》2010,39(2):16-20

用提取黄酮后废弃的花生壳做原料,选择不同的活化剂在一定温度下制备活性炭,并且测定其吸附性能。结果表明：磷酸作为活化剂时活性炭产率最高,达39.5%;当炭化温度为500℃、活化剂为氢氧化钾或磷酸、活化剂浓度为10%时,碘吸附值最高,为966.7mg/g;当炭化温度为500℃、活化剂浓度为10%时,几种活化剂制备出来的活性炭亚甲基蓝脱色力均达到40mL/g左右;与几种市售活性炭比较,花生壳活性炭碘吸附值能够满足市场需要,但是亚甲基蓝脱色力偏低;相同条件下,盐作为活化剂所制备出的活性炭对镍离子的吸附能力比较稳定。  相似文献