冻干粉针剂之注射用卵黄抗体在鸡传染方面的研究与应用     

要瑞丽 《兽药市场指南》2021,(3):7-10

鸡传染性法氏囊病是由传染性法氏囊病毒引起的一种急性、接触传染性疾病,也可叫做鸡传染性腔上囊病。该病毒属于双RNA病毒科,包括2个血清型,其中以法氏囊内淋巴细胞严重受损、法氏囊发炎、坏死、萎缩为主要特征,进而使鸡免疫机能受到障碍,从而干扰所有疫苗种类的免疫效果。  相似文献   

Study on the Change Rule of Egg Yolk Antibody in Laying Hens after First Immunization by Japanese Encephalitis Virus     

WANG Chao-qun  FANG Xu  SONG Zi-yu  HUANG Yan  PANG Jie  ZHAO Jian-guo 《中国畜牧兽医》2016,43(10):2786-2791

The purpose of this experiment was to study the immunization rule of the egg yolk antibody affected by different vaccines,immunization dose and injection ways and further to discuss the optimal immunization procedures of the laying hens for the preparation of egg yolk antibody against swine Japanese encephalitis virus.180 brown laying hens without any vaccines were selected and divided into 18 groups randomly,each group of 10 hens.Groups 1,2 were the control groups,injected with the sterile saline;Groups 3 to 10 were injected with subcutaneous or intramuscular injection,and the vaccine was injected with 0.2,0.5,1.0 and 1.5 mL successively.Groups 11 to 18 were also adopted two kinds of injection,followed by the same dose of vaccine immunization.Six eggs of each experimental group were gathered before immune day and after 3,7,10,14,18,21 and 28 days,the egg yolk antibody was extracted and the titer was determined.As a result,the egg yolk antibody titers of groups 1 to 6,11 and 12 were all 0,and no significant immune response produced;The hens from 7 to 10 groups were injected with the inactivated vaccine.After 7 days,the average antibody titer reached the peak,and the duration of the antibody was 14 days.The hens from 13 to 18 groups were injected with the attenuated virus vaccine.After 14 days,the average antibody titer reached the highest value,and the duration of the antibody was 21 days.The egg yolk antibody titers were not significantly different in the two compared experiment groups with the same injection dose but with different injection ways (P>0.05).With the same injection way of each experiment group,and the difference was significant (P>0.05).Compared with some groups with the same injection and vaccine,the titer of yolk antibody was gradually increased with the increase of the immune dose,and the difference was significant (P<0.05).The results showed that,no matter intramuscular or subcutaneous injection,in order to produce a significant immune response to hens,the immune antigen dose was 1.0 mL inactivated vaccine or 0.5 mL attenuated vaccine at least.Compared with the attenuated and inactivated vaccine,inactivated vaccine stimulated the body to produce the antibody faster,but the maintenance time was shorter;The lower dose of attenuated vaccine could stimulate the body to produce antibodies,but the speed was slower,the maintenance time was longer.  相似文献   

猪瘟病毒四种不同抗原卵黄抗体中和效力评价     

江馗语 《兽医导刊》2016,(16):210-211

本研究通过基因工程技术,在大肠杆菌中高效表达了含猪瘟病毒E2基因中抗原指数较高且含有中和性抗原域的A1/A2区以及B、C区中抗原性较高、命名为tE2的重组蛋白和猪瘟病毒重复中和表位4P1重组蛋白、4P2重组蛋白,将上述3种重组蛋白和猪瘟活疫苗与白油佐剂混合乳化作为免疫原分别免疫产蛋鸡制备抗猪瘟病毒卵黄抗体,并对抗体效价和对猪瘟病毒的中和作用进行了检测和初步研究。  相似文献   

牛轮状病毒疫苗及被动免疫抗体研究进展     

高敏  付山  赵绮欢  杨霖  贺志雄  包福祥 《中国兽医学报》2023,(11):2383-2388

牛轮状病毒(bovine rotavirus, BRV)是集约化养殖中犊牛腹泻的重要致病原之一。BRV是一种二十面体对称的双链RNA病毒,其基因组含有多种结构蛋白和非结构蛋白,并分为众多血清型,流行型多为G6和G10型,具有较强的致病性。目前,国内BRV的商品化疫苗和针对性治疗产品仍处于市场空白阶段。随着养牛业的蓬勃发展,对于BRV的防治已经迫在眉睫。对BRV的结构特点、传播途径、以及养牛业发达国家市场上的BRV疫苗上市情况和BRV卵黄抗体的研究进程进行综述,旨在为下一步疫苗和抗体的研发应用提供一定的参考。  相似文献   

再生珍珠培育新技术     

骆年华  杨燕 《科学养鱼》2006,(8):25-25

珍珠采收通常采用杀死珠蚌．从珍珠囊袋里取出珍珠的方式,该方式一只河蚌只能培育一次珍珠,其资源利用率只有一次,如想再得到珍珠,必须重新开始小蚌繁育、插种、养殖等一系列生产活动。而再生珍珠（图见彩中插2）生产是采用活蚌取珠的方式,即用开壳器将珠蚌撑开。  相似文献   

清远地区种鹅卵黄性腹膜炎的诊治与体会     

李功勇  张冰  何丽贤 《广东畜牧兽医科技》2007,32(4):21-21,23

鹅卵黄性腹膜炎,又称"蛋子瘟",是由致病性大肠埃希氏菌所引起的.本病在清远地区多个种鹅场流行.通常在产蛋初期或中期开始发生,至产蛋期结束而停止.发病率的高低因发病时所处的产蛋阶段而异.在产蛋初期发病率较高,可达25%～28%.  相似文献   

鸡新城疫病毒分子生物学特性及检测方法研究进展     

王青  张改平  赵森林  王选年  胡建和  王自良  杨苏珍  鲁晓翠  肖治军 《河南农业科学》2007,(5):114-116

鸡新城疫是由新城疫病毒(NDV)引起的一种急性败血性传染病,是危害养鸡业发展最严重的疫病之一.NDV属于副黏病毒科、副黏病毒亚科、腮腺炎病毒属,是一种有囊膜的负链RNA病毒[1].  相似文献   

嗜鞣管囊酵母木糖发酵乙醇高产突变株选育及其发酵特性     

潘丽军  储开庆  杨培周 《核农学报》2011,25(3):477-481,509

目前能够利用木糖发酵产乙醇的菌株很少且产量较低,对菌株进行诱变选育,可以提高其对木质纤维集的利用率.本试验选择嗜鞣管囊酵母As2.1585为原始菌株,采用15keV的低能氮离子进行注入诱变.随着注量的增加,菌体存活率曲线呈"马鞍型",综合考虑其存活率和正突变率,确定最佳诱变注量为12.5×1014ions/cm2,对诱...  相似文献   

多氯联苯(PCBl254)对栉孔扇贝消化盲囊和鳃丝EROD、GST酶活力的影响   总被引：4，自引：1，他引：3  

任加云  李树峰 《中国水产科学》2008,15(2):342-346

研究了4种质量浓度（0．5μg／L、1μg／L、10μg／L、50μg／L）PCB1254暴露下,栉孔扇贝（Chlamys ferrari）消化盲囊和鳃丝7-乙氧基-3-异吩唑酮-脱乙基酶（EROD）、谷胱甘肽转移酶（GST）活力的变化。于实验开始后0、0．5d、1d、3d、6d、9d、15d、21d、30d取样测定,结果显示,除鳃丝EROD活力在低质量浓度（0．5μg／L和1．0μg/L）随时间变化不明显（P〉0．05）外,其他各处理组PCB1254对栉孔扇贝消化盲囊和鳃丝的EROD都具有明显的激活作用,而且具有明显的剂量-效应关系。低浓度（0．5μg/L、1．0μg/L）处理下,消化盲囊和鳃丝的GST活力都呈现激活的趋势,而高浓度（10μg/L、50μg/L）下,GST活力都呈现先上升后下降的趋势,且鳃丝GST活力变化幅度较为明显。结果表明,EROD和GST活力的变化在一定程度上能够反应PCBs对栉孔扇贝影响的规律性,是合适的毒理学指标,栉孔扇贝是比较好的评价多氯联苯污染的指示生物,本研究也对栉孔扇贝对多氯联苯的生物转化机制作了初步的探讨。[中国水产科学,2008,15（2）：342-346]  相似文献   

犬子宫蓄脓的诊断及手术治疗报告     

潘宗海  张启迪  于占松  刘焕奇  王连珠 《畜禽业》2008,(2):76-77

<正>犬的子宫蓄脓指犬的子宫腔内贮留大量脓汁并伴有子宫内膜囊泡性增生的疾病称犬的子宫蓄脓,是发情后期的一种疾病,一般分为开放型和闭锁型子宫蓄脓2种。自2006年8月至2007年9月期间,在安丘市畜牧局宠物门诊共收治5例患有子宫蓄脓的小型犬,分别为京巴3例,博美和西施  相似文献